Performing Crush Axotomy on the Facial Nerve in a Mouse Model

Samenvatting

Source: Olmstead, D. N., et al., Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury. J. Vis. Exp. (2015).

This video demonstrates the facial nerve crush procedure in a mouse, where blunt dissection through key landmarks exposes the facial nerve trunk at the stylomastoid foramen. Forceps are then used to compress the nerve trunk, inducing axonal damage and disrupting neuronal signal transmission.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Surgical Approach

1. Align and focus the stereoscope with the surgical field. Adjust the nose cone and tape it down so that it is positioned along the edge of the visual field.
2. With the mouse lying on its left side, tape the edge of the right ear to the ....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Stereo Microscope	Leica	M60
Labeling tape	Fisher Scientific	15-952
Vannas-Tübingen Spring Scissors - Straight/Sharp/8.5cm/5mm cutting edge	Fine Science Tools	15003-08	Sterilize before use
Dumont #5/45 Forceps - Standard tips/Angled 45°/Dumoxel/11cm	Fine Science Tools	11251-35	Sterilize before use
0.9% Sodium Chloride Injection, USP	Hospira	0409-4888-10
Betadine, 16 oz, with dispenser	Fisher Scientific	19-027132
70% Ethanol
Glass Bead Sterilizer