Inducing Hypoxic-Ischemic and Inflammatory Conditions to Model Encephalopathy in Prenatal Rats

Samenvatting

Source: Jantzie, L. L., et. al. Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats. J. Vis. Exp. (2015)

This video demonstrates a protocol to induce hypoxic-ischemic and inflammatory conditions in fetal rats by restricting uterine blood flow and injecting LPS, modeling neuroinflammation and disrupted brain development relevant to preterm birth studies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Placement of Aneurysm Clips

1. Place a rat 30 G aneurysm clip on each uterine artery. Ensure cessation of blood flow, including proximal and distal pulses, and darkening of the uterine vessels, including individual placentas. Cover the exposed horns and entire surgical fiel....

Materialen

Lijst van materialen gebruikt in dit artikel

Naam	Bedrijf	Catalogusnummer	Opmerkingen
Saline Solution, 0.9%	Sigma	S8776
LPS 011B4	Sigma	L2630
Evan's Blue Dye	Sigma	E2129
Surgical gloves	Biogel	40870
OR Towels	Cardinal Health	287000-008	Sterile
PDI Alcohol Prep Pads	Fisherbrand	06-669-62
Mini Arco Rechargeable Clippers	Kent Scientific Corp.	CL8787
Blunt Forceps	Roboz	RS-8100
Scissors	Roboz	RS-6808
Surgical Scissors	Roboz	RS-5880
Surgical Scissors	F.S.T.	14002-16
Syringe	BD	309628	1 ml
Needle	BD	305122	25G 5/8
Needle	BD	305128	30G 1
Cotton-tipped Applicators	Fisherbrand	23-400-114	Small, 6 inch sterile
Cotton Gauze Sponge	Fisherbrand	22-362-178
Needle Holders	Kent Scientific Corp.	INS600109	12.5 CM STR
Vessel Clips	Kent Scientific Corp.	INS600120	30G Pressure
3-0 Perma Hand Silk Sutures	Ethicon	1684G	Black braided, 3-0 (2 metric), 18", non-absorbable, PS-1 24mm needle, 3/8 circle
Insulin Syringes	BD	328438	0.3cc 3mm 31G