Titel Cell Encapsulation door Droplets

Method Article

DOI:

⸱

October 1st, 2007

Protocol

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NIH3T3 cellen voorbereiding:

A. Cellen voor Ejection

Trypsinize cellen, dan verdunnen 1:1 met cel-media, en de transfer van een T75 kolf met een 15 ml Falcon buis
Spin down cellen in een pellet door centrifugeren, aspireren supernatant en wassen cellen met DPBS
Spin down cellen in een pellet weer, en zuig supernatans
Resuspendeer cellen in de media
Bepaal celdichtheid met hemocytometer (~ 200 X 10 ⁴ cellen / ml per fles T75)
Centrifuge cel oplossing, aspireren supernatant, en resuspendeer in de juiste hoeveelheid media voor uiteenlopende celconcentraties

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The authors have nothing to disclose.

List of materials used in this article
Name	Company	Catalog Number	Comments
Fluorescent Microscope		Nikon Instruments	Eclipse TE-2000 U
Solenoid valve cell ejector				Operation frequency: 1 KHz, 30psi, 50nl~0.5ul. 12V Valve Driver: 2.5 Amp drive current
5-Gallon Portable air tank		Coleman	Powermate CT5	Pressurized air: 30 PSI
Pressure regulators		Marsh Bellofram
Pulse Generator			HP8112A	Actuation frequency generation: 50 MHz
XYZ-stage		Newmark Systems		With Stepping motor: 6inch travel xy-stage(NLS4-6-25), 2.5inch travel z-stage(NLS4-2.5-25), 3axis controller(NSC-G3), 0.1um resolution
NIH 3T3	Cell-line			fibroblasts
Trypsin				0.05% solution
NIH 3T3 cell medium
DPBS	Buffer
T75				Tissue culture flasks
Plastic conical tubes				15 ml, for tissue culture

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