Method Article

Cardiale druk-volume curve analyse met behulp Conductance katheters in Muizen

DOI:

10.3791/52942

⸱

September 17th, 2015

 , 
1Department of Medicine, Duke University Medical Center

In This Article

Summary

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Cardiac pressure-volume loop analysis is the most comprehensive way to measure cardiac function in the intact heart. We describe a technique to perform and analyze cardiac pressure volume loops, using conductance catheters.

Abstract

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Cardiac pressure-volume loop analysis is the “gold-standard” in the assessment of load-dependent and load-independent measures of ventricular systolic and diastolic function. Measures of ventricular contractility and compliance are obtained through examination of cardiac response to changes in afterload and preload. These techniques were originally developed nearly three decades ago to measure cardiac function in large mammals and humans. The application of these analyses to small mammals, such as mice, has been accomplished through the optimization of microsurgical techniques and creation of conductance catheters. Conductance catheters allow for estimation of the blood pool by exploiting the relationship between electrical conductance and volume. When properly performed, these techniques allow for testing of cardiac function in genetic mutant mouse models or in drug treatment studies. The accuracy and precision of these studies are dependent on careful attention to the calibration of instruments, systematic conduct of hemodynamic measurements and data analyses. We will review the methods of conducting pressure-volume loop experiments using a conductance catheter in mice.

Introduction

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Cardiale druk volume curve analyse geeft gedetailleerde informatie van de hartfunctie en de gouden standaard voor functionele beoordeling 1. Terwijl beeldvormende technieken zoals echocardiografie of cardiale MRI bieden functionele maatregelen, deze maatregelen zijn sterk afhankelijk van het laden van omstandigheden. Lastonafhankelijke maatregelen van cardiale contractiliteit en relaxatie vereisen dynamische metingen van de ventriculaire druk en volume verhouding over een reeks preload en afterload. Dit begrip van de druk-volume relatie komt voort uit het baanbrekende werk van Sagawa en collega's 2,3. Ze aangetoond in ex vivo doorbl....

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Protocol

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1. conductantiecatheter Preparaten en druk kalibratie

  1. Sluit conductantiecatheter naar de hemodynamische katheter module. Elektronisch kalibreren druk- en volumemetingen door het opnemen van vooraf ingestelde druk en volume van de catheter module. Het opnemen van een tracing van 0 mm Hg en 25 mm Hg (Figuur 1A) en wijs spanningen zowel druk tracings (Figuur 1B en 1C). Ook een volume tracering van 5 RVU en 25 RVU (figuur 1D) opnemen en deze toewijzen spanningen zowel volume tracings (1E en 1F).
  2. Bevestig de elektronische druk kalibratie met een manuele druk kalibratie, met behulp van een kwikkolom bl....

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Results

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De druk-volume curve analyse kan worden gebruikt om de hartfunctie bij genetisch gemodificeerde muizen of muizen ondergaan 14,15 drug studies 16 meten. Representatieve druk volume lussen zijn voorzien van het eerder gepubliceerde werk 16 onderzoeken van het effect van ß-arrestine bevooroordeeld AT1R ligand, TRV120023. Om te testen of TRV120023 invloed op de hartfunctie in vivo, werd de druk-volume lus analyse uitgevoerd op wild-type muizen die conventionele en nieuwe angiotensin.......

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Discussion

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We beschrijven een werkwijze voor de druk-volume curve analyse perfoming met een conductantiecatheter bij muizen, uitgebreide analyses van zowel cardiale contractiliteit en relaxatie ontlenen. Suga, Sagawa en collega benut druk-volume lussen maatregelen van cardiale contractiliteit bijzonder de helling van de ESPVR of eind-systolische elastantie (E sen) en Emax definiëren. Elasticiteit, bepaald door de verhouding van druk op volume (P / V) varieert over de duur van systole. Tijdens elke systole, de.......

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Disclosures

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De auteurs hebben niets te onthullen.

Acknowledgements

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Dit werk wordt ondersteund door de American Heart Association 14FTF20370058 (DMA) en NIH T32 HL007101-35 (DMA).

....

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Materials

List of materials used in this article
NameCompanyCatalog NumberComments
AnaSed (xylazine) Lloyd LaboratoriesNADA no. 139-236Anesthetic
Ketaset (ketamine)Pfizer440842Anesthetic
VIP3000Matrx Medical Inc.Anesthesia machine
VentilatorHarvard ApparatusModel 683Surgical Equipment
Tubing kitHarvard Apparatus72-1049Surgical Equipment
Homeothermic Blanket Kaz Inc.5628Surgical Equipment
Stereo microscopeCarl Zeiss Optical Inc.Stemi 2000Surgical Equipment
IlluminatorCole–Parmer41720Surgical Equipment
Dumont no. 55 Dumostar Forceps Fine Science Tools Inc11295-51Surgical Instruments
Graefe forceps, curved Fine Science Tools Inc11052-10Surgical Instruments
Moria MC31 forceps Fine Science Tools Inc11370-31Surgical Instruments
Mayo scissors Fine Science Tools Inc14512-15Surgical Instruments
Iris scissors Fine Science Tools Inc14041-10Surgical Instruments
Halsey needle holder Fine Science Tools Inc12501-13Surgical Instruments
Olsen–Hegar needle holder Fine Science Tools Inc12002-12Surgical Instruments
spring scissorsFine Science Tools Inc15610-08Surgical Instruments
disposable underpadsKendall/Tyco Healthcare1038Surgical Supplies
Sterile gauze sponges, sterile Dukal62208Surgical Supplies
Cotton-tipped applicators, sterile Solon368Surgical Supplies
Surgical suture,  silk, 6-0 DemeTECHFT-639-1Surgical Supplies
1 cc Insulin syringes Becton Dickenson329412Surgical Supplies
Access 9 Hemostasis ValveMerit Medical MAP111Hemodynamic equipment
SphygmomanometerBaumanometer320Hemodynamic equipment
Millar PV system MPVS-300/400 or MPVS Ultra (includes calibration cuvette)ADInstruments IncHemodynamic equipment
1.4F conductance catheter ADInstruments IncSPR-839Hemodynamic equipment
PowerLab 4/30 with Chart ProADInstruments Inc.ML866/PHemodynamic software
animal clipperWahl8787-450AMiscellaneous
Intradermic tubing PE-10Becton Dickenson427401Miscellaneous
Intradermic tubing PE-50Becton Dickenson427411Miscellaneous
Needle assortment (18, 25 and 30 gauge; Thomas Scientific)Miscellaneous
0.9% (wt/vol) sodium chloride injection, USP)HospiraNDC no. 0409-4888-50Miscellaneous
Surgical tapeMiscellaneous
Alconox (Alconox Inc.) for catheter cleaningADInstruments Inc.Miscellaneous

References

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  1. Clark, J. E., Marber, M. S. Advancements in pressure-volume catheter technology - stress remodelling after infarction. Exp Physiol. 98 (3), 614-621 (2013).
  2. Sunagawa, K., Maughan, W. L., Burkhoff, D., Sagawa, K. Left ventricular interaction with ....

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Tags

Pressure Volume Loop AnalysisConductance Catheter MiceCardiac Function AssessmentVentricular Systolic DiastolicAortic Occlusion TechniqueInferior Vena CavaHemodynamic Measurements MiceCalibration Conductance CatheterLoad Dependent IndependentGenetic Mutant Models

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