Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Samenvatting

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Samenvatting

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Protocol

Er is een erratum gepubliceerd voor Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

De menselijke RCTEC/RCTE-celstammen die hier en in Caralt et al., 2015, Uzarski et al., 2015 zijn gebruikt, werden oorspronkelijk in 2001 door Dr. Loghman-Adham (destijds aan St. Louis University) via een MTA verstrekt aan co-auteur Dr. Wandinger-Ness (University of New Mexico). De SV40-geïmmortaliseerde menselijke RCTEC/RCTE-cellen werden gekarakteriseerd als een distale tubulus-cellijn, zoals gedetailleerd in Loghman-Adham et al., 2003. Op basis van recente short tandem repeat (STR) DNA-sequencing (uitgevoerd door IDEXX BioResearch) van de vroegste passages van de RCTEC/RCTE-celstammen werd duidelijk dat de cellen van een gemengde afkomst waren. Verdere analyses van PCR-producten met behulp van QIAxcel capillaire elektroforese toonden de aanwezigheid van een canien product aan. Het product werd gesequenced en bleek van caniene oorsprong te zijn. Een set caniene specifieke STR-markers werd vergeleken met het monster en toonde aan dat het monster een genetisch profiel had met 92% identiteit met de MDCK-cellijn.  Latere celstammen die in de huidige publicatie zijn gebruikt, werden STR-geprofileerd en vertoonden een drift naar een 100% MDCK-afkomst. Ondanks de caniene oorsprong, in plaats van menselijk zoals voorheen werd gedacht, is MDCK eveneens een epitheliale cellijn van de distale tubulus. Om deze reden blijven eerdere interpretaties en conclusies getrokken met deze cellen hier en in Caralt et al., 2015, Uzarski et al., 2015 valide, maar om redenen van striktheid, reproduceerbaarheid en experimentele validatie door anderen rapporteren wij deze misidentificatie. Deze informatie zal ook worden gerapporteerd voor opname in de database van het International Cell Line Authentication Committee (ICLAC) (http://iclac.org).