Bacterial Enrichment
Bacterial Enrichment - Related Videos
04:16
Enrichment of Bacterial Lipoproteins Using Non-Ionic Detergent Phase Separation
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2025
Source: Armbruster, K. M., Meredith, T. C. Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry. J. Vis. Exp. (2018)This video demonstrates the enrichment of bacterial lipoproteins using a non-ionic detergent that undergoes phase separation upon warming. Through sequential temperature shifts and centrifugation steps, lipoproteins are selectively partitioned into the detergent phase, while non-lipoprotein contaminants...
10:44
Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
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Cited by 1 •
2025
Here, we present a protocol to enable whole-genome sequencing of bacterial sexually transmitted infections from clinical samples using target enrichment. This novel, syndromic panel-based method overcomes challenges of abundant human DNA and low bacterial loads, facilitating genomic surveillance for Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, and Mycoplasma genitalium.
01:34
Environmental Enrichment for Rodents
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Environmental enrichment involves objects and practices that encourage natural behaviors, improve psychological well-being, and promote better welfare in laboratory rodents. Environmental enrichment should be tailored to the...
02:03
Enrichment of Anode-Respiring Bacteria Using an On-Site Electrochemical System
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2025
Source: Okamoto, A., et al. Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site. J. Vis. Exp. (2018)This video demonstrates the use of an on-site electrochemical system to enrich anode-respiring bacteria (ARB) from natural reservoirs. A redox gradient is established by placing the anode in oxygen-free deep water and the cathode in oxygen-rich surface water. ARB form biofilms and transfer electrons to the anode, which flows to the cathode, where oxygen is reduced,...
03:57
Bacterial Infection Quantitation Assay: A Method to Quantify Intraocular Bacterial Load in Mouse Model of Bacterial Endophthalmitis
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2025
This video describes the technique of quantifying bacterial infection from eye homogenates obtained from a murine model of bacterial endophthalmitis caused by Bacillus cereus. This technique helps study the host immune response and its dependency on the host and ocular bacterial gene expression in murine models.
FAQs
The chosen environment changes the balance between the target bacteria and competing microbes. Nutrient availability can favor organisms able to use particular resources, while temperature, pH, and oxygen availability further influence which populations persist and increase in relative abundance. Selective inhibitors add another level of control by limiting organisms that would otherwise compete with the bacteria under investigation.
Each condition targets a different aspect of microbial growth and survival. Specific nutrients support bacteria with matching physiological capabilities, oxygen availability favors organisms suited to the selected oxygen environment, and pH or temperature can shift community composition. Selective inhibitors help reduce competing microbes, improving the likelihood that low-abundance target organisms become detectable.
Working directly with a mixed sample may make low-abundance bacteria difficult to detect or separate from more numerous organisms. Enrichment first shifts the sample toward the selected population, creating a culture in which the target bacteria are relatively more abundant. This supports subsequent detection, isolation, identification, physiological analysis, and molecular testing.
A typical workflow begins by placing the mixed sample into an enrichment medium or environment designed around the target organisms. The culture is then maintained under selected nutrient, temperature, pH, oxygen, or inhibitor conditions. After enrichment, the resulting culture can be examined for detection or used for isolation, identification, physiological analysis, and molecular testing.
Planning requires identifying conditions likely to favor the bacteria of interest while limiting competing microbes. Researchers consider the available nutrients, incubation temperature, pH, oxygen availability, and use of selective inhibitors. These variables determine the biological pressures applied to the mixed sample, so the resulting culture reflects the chosen enrichment strategy rather than the original community alone.
The approach is valuable when a target organism occurs at low abundance in a complex sample. It can support recovery from environmental, clinical, food, and industrial materials, then provide cultures for identification or physiological analysis. Researchers can also use enriched cultures for molecular testing and for investigating microbial functions or interactions within the selected community.