Tear Volume Quantification
Tear Volume Quantification - Related Videos
01:46
Quantification of Tear Volume in an Autoimmune Dry Eye Rat Model using the Phenol Red Thread
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2025
This video demonstrates the phenol red thread test to determine tear volume in an autoimmune dry eye rat model. The pH-sensitive acidic phenol red thread changes color when it comes in contact with the alkaline tears. The shorter length of color change in the phenol red thread corresponds to reduced tear volume.
07:45
Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy
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Cited by 1 •
2025
This article describes a protocol for the collection and detection of miR-15a from tears as a new diagnostic tool for diabetic retinopathy.
05:57
Sex Hormone Detection In Human Tear Film Using Enzyme-Linked Immunosorbent Assays
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2026
This protocol describes an optimized enzyme-linked immunosorbent assay (ELISA) method to detect and quantify 17β-estradiol and testosterone in human tear fluid collected via microcapillary tubes. This approach enables minimally invasive measurement of tear hormone levels.
12:45
Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
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Cited by 10 •
2022
Tunneling nanotubes (TNTs) are primarily open-ended F-actin membrane nanotubes that connect neighboring cells, facilitating intercellular communication. The notable characteristic that distinguishes TNTs from other cell protrusions is the hovering nature of the nanotubes between cells. Here, we characterize TNTs by constructing a 3D volume view of confocal z-stack images.
04:38
A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome
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Cited by 4 •
2011
Capsulorhexis is an important step in phacoemulsification surgery. A surgeon creates a continous curvilinear tear on the anterior lens capsule by controlling the tearing vector forces. A peripherally extended tear is a serious complication. This video demonstrates a novel technique of rescuing capsular radial tear out using a cystotome.
FAQs
A defined collection interval establishes the time basis for comparison, while the device provides the readout. With an absorbent strip, the wetted length estimates how much lacrimal fluid entered the material. A microcapillary device instead provides a calibrated reading. These measurements link tear production with ocular surface hydration and its role in defense.
Reflex tearing can increase the collected volume beyond the fluid present under the intended conditions, whereas sample loss can reduce the measured amount. Both effects weaken comparisons between experiments or samples. Minimizing these disturbances helps the recorded value more accurately reflect lacrimal fluid production and clearance rather than collection-related changes.
Inflammation, allergy, microbial exposure, and disease can change tear production or clearance, producing differences in measured volume. The result therefore serves as a functional indicator alongside other observations. In immunology and infection research, comparing these changes can help relate ocular-surface responses to altered hydration and defense.
First, establish a defined collection period and use the selected collection device while limiting reflex tearing. Next, retain the collected fluid and avoid sample loss. For an absorbent strip, estimate volume from the wetted length; for a microcapillary device, record the calibrated reading. Consistent handling supports reliable comparison across samples.
Both approaches collect lacrimal fluid over a defined period, but their measurement outputs differ. An absorbent strip translates the wetted length into an estimated volume, whereas a microcapillary device supplies a calibrated reading. Keeping the collection conditions consistent remains important because the device-specific readout must still represent comparable tear production or clearance.
Collected tear fluid can support analysis of antibodies, cytokines, antimicrobial proteins, and pathogen-associated signals. Pairing these measurements with tear volume provides functional context for interpreting ocular-surface inflammation, allergy, microbial exposure, or disease. The combined information can help distinguish changes in fluid handling from changes in immune or infection-related components.