Saline Irrigation

Saline irrigation is the controlled washing of a body surface, wound, or surgical field with sterile salt solution, a routine practice that helps maintain a clean, moist environment. In neuroscience and neurosurgery, isotonic saline is applied to remove blood and tissue debris, prevent desiccation, and support visualization while limiting disruption of cells through osmotic balance. It is used during cranial and spinal procedures, including microsurgery and experimental tissue preparation, where careful flow and temperature management can help protect exposed neural structures and preserve the quality of the operative field. Proper technique therefore contributes to safer manipulation, clearer anatomy, and reliable research or clinical outcomes.

Saline Irrigation - Related Videos

Research

JoVE EoE - Neuropathology

A Hypertonic Saline Injection Technique for Inducing Glaucoma in Rats

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2025

This video demonstrates glaucoma induction in an anesthetized rat by injecting hypertonic saline into the episcleral vein. The saline enters the trabecular meshwork via Schlemm's canal, causing tissue scarring, which blocks aqueous humor outflow and raises intraocular pressure. The elevated pressure damages retinal ganglion cells, leading to glaucoma.

Salinity Tolerance of Alishewanella Species Isolated from Seawall Muddy Water

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2025

Begin with a culture of Alishewanella, a Gram-negative, salt-tolerant bacterium.Next, streak the culture onto agar plates containing increasing concentrations of sodium chloride to simulate varying levels of saline stress.Overlay each plate with a layer of unsolidified medium to prevent oxygen diffusion and maintain the culture in an oxygen-limited environment.Seal and incubate the plates at an optimum temperature to support bacterial growth.During incubation, the bacterium exhibits varying...

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

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Cited by 2 •

2016

Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.

Education

JoVE Core - Civil Engineering

Design Example: Design of an Irrigation Channel

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2025

Trapezoidal channels are widely used in irrigation systems due to their cost-effectiveness and efficiency in conveying water. Trapezoidal channels feature a flat bottom and sloping sides, making them stable and easier to construct compared to other shapes. The bottom width and side slope ratio are determined based on the required flow capacity and site conditions. The side slope is kept gentle for unlined channels to prevent soil erosion.Hydraulic parameters in channel design include the flow...

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

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2024

Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.

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