Imaging Bacterial Localization and Host Interaction in Human Lung Tissue

Take a fluorescently labeled Staphylococcus aureus suspension and human alveolar lung tissue infected with the same fluorescently labeled bacteria.

Insert the distal tip of the fibered confocal fluorescence microscopy probe into the bacterial suspension and scan.

Bright fluorescent signals confirm bacterial membrane labeling and successful probe activation

Record the fluorescence as the probe moves through the suspension.

Using a hydrogen peroxide-soaked lens clearing tissue, wipe the probe to remove residual bacteria and prevent background fluorescence

Bring the cleaned probe into direct contact with the tissue and scan the surface.

In the tissue, collagen and elastin fibers appear as an autofluorescent matrix, while fluorescently labeled bacteria are visible as distinct bright spots.

The alveolar spaces, lacking fluorescence, appear as dark regions.

Record a video to capture both tissue-adhered and loosely associated bacteria, enabling real-time visualization of bacterial infection in host alveolar lung tissue.

To begin data collection, click start on screen or press the left foot pedal to turn on the laser. Next, image each of the bacterial suspension samples. Insert the distal end of th