Question 1

What is the difference between mechanical and electronic analytical balances?

Accepted Answer

Mechanical balances like triple beam balances use counterweights on parallel beams to restore equilibrium when a sample is placed on the pan. Modern electronic analytical balances use electromagnetic compensation, where a spring displacement triggers a load cell to send electrical current to a signal processor that displays mass. Electronic balances are more precise, measuring down to ten thousandths or even hundred thousandths of a gram.

Question 2

Why is a draft shield important when using an analytical balance?

Accepted Answer

The draft shield is a glass enclosure with movable doors that prevents air currents and dust from contacting the weighing pan. Air currents can disrupt the balance and cause inaccurate measurements. Closing the draft shield during weighing ensures stable readings and protects the sensitive instrument from environmental interference.

Question 3

How should you prepare a substance before weighing it on an analytical balance?

Accepted Answer

For hygroscopic substances that absorb moisture, weigh them quickly. Always allow frozen or refrigerated substances to reach room temperature first, since cold chemicals absorb moisture and weigh more than warmed samples, causing incorrect measurements. Consult regulating temperature in the lab preserving samples using cold guidance for proper storage techniques.

Question 4

What is the purpose of tarring an analytical balance before weighing?

Accepted Answer

Tarring resets the scale to read zero after placing a weigh boat or weigh paper on the weighing pan. This subtracts the mass of the container so only the substance's mass is measured. Press the tare bar to re-zero the scale, ensuring accurate measurement of the sample alone without container weight.

Question 5

When should you use weigh paper versus a weigh boat?

Accepted Answer

Use weigh paper for smaller amounts or when the substance will be transferred into a small container like a vial or microfuge tube. Weigh paper can be folded for easy handling. Use weigh boats when larger quantities need to be weighed. Both protect the weighing pan from dirt and chemical damage.

Question 6

How does calibration help maintain analytical balance accuracy?

Accepted Answer

Calibration is the process of adjusting a balance's output against an object of known weight. Analytical balances often come with calibration weights—standard weights with precise, specific mass. Periodically checking the balance against these weights verifies the instrument is giving accurate readings and identifies any drift in measurement performance.

Question 7

What are common laboratory applications for analytical balances beyond weighing chemicals?

Accepted Answer

Analytical balances weigh liquids for centrifugation experiments, where ultracentrifuge tubes must have identical mass to keep the rotor balanced. Scientists also weigh animals and insects like drosophila and mosquitoes to track body weight and food consumption throughout studies. Understanding centrifuge principle instrumentation and applications helps contextualize these measurements.

Het meten van massa in het laboratorium - Beoordelingsquiz