Modelleren van osteosarcoom met behulp van Li-Fraumeni syndroom geïnduceerde pluripotente stamcellen patiënt-afgeleide cellen

8.4K weergaven

Geciteerd door 14

08:52 min.

13 juni 2018

10.3791/57664-v

13 juni 2018

8.4K weergaven

Ruoji Zhou¹^,² , An Xu¹ , Jian Tu¹^,³ , Mo Liu¹ , Julian A Gingold⁴ , Ruiying Zhao¹ , Dung-Fang Lee¹^,²^,⁵^,⁶

¹Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, ²Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, ³Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, ⁴Women's Health Institute, Cleveland Clinic Foundation, ⁵Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, ⁶Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

Hier presenteren we een protocol voor het genereren van geïnduceerde pluripotente stamcellen (iPSCs) van Li-Fraumeni syndroom (LFS) patiënt afgeleide fibroblasten, differentiatie van iPSCs via mesenchymale stamcellen (MSCs) botcellen te modelleren in vivo tumorigenesis met behulp van LFS patiënt afkomstige botcellen.

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iPSC-differentiatie

mesenchymale stamcellen

osteoblastdifferentiatie

in vivo tumorigenese

alkalische fosfatasekleuring

alizarinerood S-kleuring

immunofluorescentiekleuring

subcutane injectie

naaktmuismodel

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