Monstervoorbereiding tot bio-informatica analyse van DNA-methylatie: associatiestrategie voor obesitas en gerelateerde eigenschappenstudies

6.3K weergaven

Geciteerd door 9

14:56 min

6 mei 2022

10.3791/62598-v

6 mei 2022

6.3K weergaven

Natália Yumi Noronha^*¹ , Guilherme da Silva Rodrigues^*¹ , Marcela Augusta de Souza Pinhel¹ , Jean-Baptiste Cazier²^,³ , Lígia Moriguchi Watanabe¹ , Albert Nobre Menezes⁴ , Carlos Roberto Bueno⁵ , Carolina Ferreira Nicoletti¹ , Bruno Affonso Parenti de Oliveira¹ , Isabelle Mello Schineider⁶ , Isabella Harumi Yonehara Noma⁷ , Igor Caetano Dias Alcarás⁸ , Fernando Barbosa⁹ , Carla Barbosa Nonino⁶

¹Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, ²Centre for Computational Biology, University of Birmingham, ³Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, ⁴Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, ⁵Ribeirão Preto School of Physical Education and Sport, University of São Paulo, ⁶Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, ⁷Faculty of Pharmaceutical Sciences, University of São Paulo, ⁸Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, ⁹Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo

^* These authors contributed equally

De huidige studie beschrijft de workflow om DNA-methylatiegegevens te beheren die zijn verkregen door microarray-technologieën. Het protocol demonstreert stappen van monstervoorbereiding tot data-analyse. Alle procedures worden in detail beschreven en de video toont de belangrijke stappen.