Name: Genereren van weefselsferoïden via een 3D-geprint stempelachtig apparaat (Video) | JoVE Journal
Uploaded: 2022-10-06T12:40:00.000+00:00
Duration: 6 min 39 s
Description: Leer hoe u weefselsferoïden produceert met een 3D-geprint stempelapparaat voor tissue-engineering en geneesmiddelontwikkeling. Deze methode garandeert hoge celviabiliteit en uniforme kwaliteit.

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<table><tbody><tr><td>6 well plate</td><td>Merck</td><td>CLS3516</td><td></td></tr><tr><td>Agarose</td><td>Promega</td><td>V3121</td><td></td></tr><tr><td>Biodevice&nbsp;</td><td>Bioedtech</td><td></td><td></td></tr><tr><td>Biological Safety Cabinet</td><td>ThermoFisher&nbsp;</td><td>51029701</td><td></td></tr><tr><td>Centrifugue</td><td>ThermoFisher&nbsp;</td><td>75004031</td><td></td></tr><tr><td>Corning 50 mL centrifuge tubes</td><td>Merck</td><td>CLS430829-500EA</td><td></td></tr><tr><td>Corning cell culture flasks surface area 75 cm2</td><td>Merck</td><td>CLS430641</td><td></td></tr><tr><td>Draft Resin&nbsp;</td><td>FormLabs</td><td>FLDRBL01</td><td></td></tr><tr><td>Dulbecco&prime;s Modified Eagle&prime;s Medium - low glucose</td><td>Merck</td><td>D6046</td><td></td></tr><tr><td>Fetal Bovine Serum (FBS)</td><td>ThermoFisher&nbsp;</td><td>16000044</td><td></td></tr><tr><td>Form 2</td><td>FormLabs</td><td></td><td></td></tr><tr><td>Incubator</td><td>ThermoFisher&nbsp;</td><td>51033782</td><td></td></tr><tr><td>L929 cell lines</td><td>Stablished in the lab&nbsp;</td><td></td><td></td></tr><tr><td>Penicillin and Streptomycin (PS)</td><td>ThermoFisher&nbsp;</td><td>15140122</td><td></td></tr><tr><td>Phosphate-Buffered Saline (PBS)</td><td>Merck</td><td>806552</td><td></td></tr><tr><td>Trypsin with EDTA</td><td>Merck</td><td>T3924</td><td></td></tr></tbody></table>

genereren van weefselsferoïden via een 3d-geprint stempelachtig apparaat

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Genereren van weefselsferoïden via een 3D-geprint stempelachtig apparaat

Advances in 3D cell culture have developed more physiologically relevant in vitro models, such as tissue spheroids. Cells cultivated as spheroids have ...

generation-of-tissue-spheroids-via-a-3d-printed-stamp-like-device

Research

JoVE Journal

Biochemistry

Biochemie

Beschrijven we een eenvoudige, snelle methode van het genereren van 3D weefsel-achtige sferoïden en hun potentiële toepassing op verschillen in de cel-cel interacties te kwantificeren.

A Simple Opknoping Drop Cultuur van de Cel Protocol voor het genereren van 3D-sferoïden

Spheroids are a type of 3D cell culture model used to more accurately represent a cell’s environment compared to 2D cell culture monolayer models. In the example protocol, we will see scientists generate spheroids from a colorectal cancer cell line and apply live-cell imaging to track the spheroids’ growth.

Spheroid Generation from Cell Lines: A Three-Dimensional (3D) Cell Culture Method

This video demonstrates the transformation of human pluripotent stem cells (hPSCs) into spherical brain organoids. By using specific inhibitors, the hPSCs are guided to differentiate towards a neuronal lineage, forming neuroectodermal colonies. Special growth factors are then introduced, which encourage the self-organization and proliferation of the colonies, ultimately leading to the formation of spherical brain organoids.

Generation of Spheroids from Human Pluripotent Stem Cells

Dit protocol beschrijft 3D bioprinting van hartweefsel zonder het gebruik van biomaterialen. 3D bioprinted cardiac patches vertonen mechanische integratie van component sferoïden en zijn zeer veelbelovend in hartweefselregeneratie en als 3D-modellen van hart-en vaatziekten.

Het creëren van cardiale weefsels die mechanische integratie van sferoïden tonen met behulp van 3D bioprinting

We presenteren een centrifugale microfluïdisch apparaat met motoraandrijving dat celsferioïden kan cultiveren. Met behulp van dit apparaat kunnen sferoïden van enkelvoudige of meervoudige celtypen gemakkelijk onder hoge zwaartekracht worden gecocultureerd.

Lab-op-een-CD-platform voor het genereren van multicellulaire driedimensionale Spheroids

Het protocol beschrijft high-throughput sferoïde generatie voor bioprinting met behulp van de multi-parametrische analyse van hun oxygenatie en celdood op een standaard fluorescentiemicroscoop. Deze aanpak kan worden toegepast om de levensvatbaarheid van de sferoïden te controleren en standaardisatie uit te voeren, wat belangrijk is bij het modelleren van 3D-weefsel, tumormicro-omgeving en succesvolle (micro)weefselbiofabricage.

Betaalbare zuurstofmicroscopie-geassisteerde biofabricage van meercellige sferoïden

Hier beschrijven we de grootschalige productie van van vetweefsel afgeleide stromale / stamcel (ASC) sferoïden met behulp van een geautomatiseerd pipetteersysteem om de celsuspensie te zaaien, waardoor homogeniteit van sferoïde grootte en vorm wordt gegarandeerd. Deze ASC-sferoïden kunnen worden gebruikt als bouwstenen voor 3D-bioprintingbenaderingen.

Grootschalige, geautomatiseerde productie van van vetweefsel afgeleide stamcelsferoïden voor 3D-bioprinting

Dit artikel demonstreert een gestandaardiseerde methode voor het construeren van driedimensionale tumorsferoïden. Een strategie voor sferoïde observatie en beeldgebaseerde deep-learning analyse met behulp van een geautomatiseerd beeldvormingssysteem wordt ook beschreven.

Generatie van 3D-tumorsferoïden voor geneesmiddelenevaluatiestudies

Deze studie presenteert een kosteneffectieve en efficiënte methodologie voor het genereren van 3D-celstructuren met behulp van een op stempels gebaseerd systeem om microwells in agaroseschimmels te maken. Het systeem bevordert de vorming van uniforme sferoïden/organoïden, waardoor de celinteracties worden verbeterd. Deze aanpak vermindert de experimentele variabiliteit en ondersteunt toepassingen in het testen van geneesmiddelen en weefselmanipulatie.

Generatie van driedimensionale sferoïden/organoïden uit tweedimensionale celculturen met behulp van een nieuw stempelapparaat

Proline-proline endopeptidase-1 (PPEP-1) is een uitgescheiden metalloprotease en veelbelovend geneesmiddel-doelwit van de humane ziekteverwekker Clostridium difficile. Hier beschrijven we alle methoden die nodig zijn voor de productie en structuurbepaling van dit eiwit.

Productie, kristallisatie en structuur bepalen van<em&gt; C. difficile</em&gt; PPEP-1 via Microseeding en zink-SAD

Leer hoe u weefselsferoïden produceert met een 3D-geprint stempelapparaat voor tissue-engineering en geneesmiddelontwikkeling. Deze methode garandeert hoge celviabiliteit en uniforme kwaliteit.

Genereren van weefselsferoïden via een 3D-geprint stempelachtig apparaat

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