Name: Ultrastructurele expansiemicroscopie in drie in vitro levenscyclusstadia van Trypanosoma cruzi (Video) | JoVE Journal
Uploaded: 2023-05-12T14:50:00.000+00:00
Duration: 9 min 45 s
Description: Leer ultrastructuur-expansiemicroscopie toe te passen op drie in vitro levenscyclusstadia van Trypanosoma cruzi. Deze methode maakt nanoschaalbeeldvorming mogelijk met standaard microscopen voor gedetailleerde 3D-reconstructies van cellulaire structuren.

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<meta charset="utf8"></head><body>Ultrastructurele expansiemicroscopie in de in vitro levenscyclus van Trypanosoma cruzi

<ol><li>Chen, F., Tillberg, P. W., Boyden, E. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Expansion+microscopy%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Expansion microscopy.</a> Science. 347 (6221), 543-548 (2015).</li>
<li>Chozinski, T. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Expansion+microscopy+with+conventional+antibodies+and+fluorescent+proteins%5BTitle%5D)+AND+%22Nature+Methods%22%5BJournal%5D)">Expansion microscopy with conventional antibodies and fluorescent proteins.</a> Nature Methods. 13 (6), 485-488 (2016).</li>
<li>Ku, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multiplexed+and+scalable+super-resolution+imaging+of+three-dimensional+protein+localization+in+size-adjustable+tissues%5BTitle%5D)+AND+%22Nature+Biotechnology%22%5BJournal%5D)">Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues.</a> Nature Biotechnology. 34 (9), 973-981 (2016).</li>
<li>Gambarotto, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Imaging+cellular+ultrastructures+using+expansion+microscopy+%28U-ExM%29%5BTitle%5D)+AND+%22Nature+Methods%22%5BJournal%5D)">Imaging cellular ultrastructures using expansion microscopy (U-ExM).</a> Nature Methods. 16 (1), 71-74 (2018).</li>
<li>Tillberg, P. W., Chen, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Expansion+microscopy%3A+Scalable+and+convenient+super-resolution+microscopy%5BTitle%5D)+AND+%22Annual+Review+of+Cell+and+Developmental+Biology%22%5BJournal%5D)">Expansion microscopy: Scalable and convenient super-resolution microscopy.</a> Annual Review of Cell and Developmental Biology. 35, 683-701 (2019).</li>
<li>Jurriens, D., van Batenburg, V., Katrukha, E. A., Kapitein, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mapping+the+neuronal+cytoskeleton+using+expansion+microscopy%5BTitle%5D)+AND+%22Methods+in+Cell+Biology%22%5BJournal%5D)">Mapping the neuronal cytoskeleton using expansion microscopy.</a> Methods in Cell Biology. 161, 105-124 (2021).</li>
<li>Alonso, V. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ultrastructure+expansion+microscopy+%28U-ExM%29+in+Trypanosoma+cruzi%3A+localization+of+tubulin+isoforms+and+isotypes%5BTitle%5D)+AND+%22Parasitology+Research%22%5BJournal%5D)">Ultrastructure expansion microscopy (U-ExM) in Trypanosoma cruzi: localization of tubulin isoforms and isotypes.</a> Parasitology Research. 121 (10), 3019-3024 (2022).</li>
<li>Kalichava, A., Ochsenreiter, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ultrastructure+expansion+microscopy+in+Trypanosoma+brucei%5BTitle%5D)+AND+%22Open+Biology%22%5BJournal%5D)">Ultrastructure expansion microscopy in Trypanosoma brucei.</a> Open Biology. 11 (10), 210132(2021).</li>
<li>Amodeo, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+the+novel+mitochondrial+genome+segregation+factor+TAP110+in+Trypanosoma+brucei%5BTitle%5D)+AND+%22Journal+of+Cell+Science%22%5BJournal%5D)">Characterization of the novel mitochondrial genome segregation factor TAP110 in Trypanosoma brucei.</a> Journal of Cell Science. 134 (5), (2021).</li>
<li>Liffner, B., Absalon, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Expansion+microscopy+reveals+plasmodium+falciparum+blood-stage+parasites+undergo+anaphase+with+a+chromatin+bridge+in+the+absence+of+mini-chromosome+maintenance+complex+binding+protein%5BTitle%5D)+AND+%22Microorganisms%22%5BJournal%5D)">Expansion microscopy reveals plasmodium falciparum blood-stage parasites undergo anaphase with a chromatin bridge in the absence of mini-chromosome maintenance complex binding protein.</a> Microorganisms. 9 (11), 2306(2021).</li>
<li>Dos Santos Pacheco, N., Soldati-Favre, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coupling+auxin-inducible+degron+system+with+ultrastructure+expansion+microscopy+to+accelerate+the+discovery+of+gene+function+in+Toxoplasma+gondii%5BTitle%5D)+AND+%22Methods+in+Molecular+Biology%22%5BJournal%5D)">Coupling auxin-inducible degron system with ultrastructure expansion microscopy to accelerate the discovery of gene function in Toxoplasma gondii.</a> Methods in Molecular Biology. 2369, 121-137 (2021).</li>
<li>Lidani, K. C. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chagas+disease%3A+From+discovery+to+a+worldwide+health+problem%5BTitle%5D)+AND+%22Frontiers+in+Public+Health%22%5BJournal%5D)">Chagas disease: From discovery to a worldwide health problem.</a> Frontiers in Public Health. 7, 166(2019).</li>
<li>Carrea, A., Diambra, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Systems+biology+approach+to+model+the+life+cycle+of+Trypanosoma+cruzi%5BTitle%5D)+AND+%22PloS+One%22%5BJournal%5D)">Systems biology approach to model the life cycle of Trypanosoma cruzi.</a> PloS One. 11 (1), e0146947(2016).</li>
<li>Vidal, J. C., De Souza, W. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aVidal%2C+JC+%22Morphological+and+Functional+Aspects+of+Cytoskeleton+of+Trypanosomatids.+Cytoskeleton+-+Structure%2C+Dynamics%2C+Function+and+Disease%22">Morphological and Functional Aspects of Cytoskeleton of Trypanosomatids. Cytoskeleton - Structure, Dynamics, Function and Disease</a>. , InTech. (2017).</li>
<li>Kunz, T. C., Götz, R., Gao, S., Sauer, M., Kozjak-Pavlovic, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+expansion+microscopy+to+visualize+and+characterize+the+morphology+of+mitochondrial+cristae%5BTitle%5D)+AND+%22Frontiers+in+Cell+and+Developmental+Biology%22%5BJournal%5D)">Using expansion microscopy to visualize and characterize the morphology of mitochondrial cristae.</a> Frontiers in Cell and Developmental Biology. 8, 617(2020).</li>
<li>Laporte, M. H., Klena, N., Hamel, V., Guichard, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Visualizing+the+native+cellular+organization+by+coupling+cryofixation+with+expansion+microscopy+%28Cryo-ExM%29%5BTitle%5D)+AND+%22Nature+Methods%22%5BJournal%5D)">Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).</a> Nature Methods. 19 (2), 216-222 (2022).</li>
<li>Sahabandu, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Expansion+microscopy+for+the+analysis+of+centrioles+and+cilia%5BTitle%5D)+AND+%22Journal+of+Microscopy%22%5BJournal%5D)">Expansion microscopy for the analysis of centrioles and cilia.</a> Journal of Microscopy. 276 (3), 145-159 (2019).</li>
<li>Wen, G., Leen, V., Rohand, T., Sauer, M., Hofkens, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Current+progress+in+expansion+microscopy%3A+chemical+strategies+and+applications%5BTitle%5D)+AND+%22Chemical+Reviews%22%5BJournal%5D)">Current progress in expansion microscopy: chemical strategies and applications.</a> Chemical Reviews. 123 (6), 3299-3323 (2023).</li>
<li>Trinks, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Subdiffraction-resolution+fluorescence+imaging+of+immunological+synapse+formation+between+NK+cells+and+A.+fumigatus+by+expansion+microscopy%5BTitle%5D)+AND+%22Communications+Biology%22%5BJournal%5D)">Subdiffraction-resolution fluorescence imaging of immunological synapse formation between NK cells and A. fumigatus by expansion microscopy.</a> Communications Biology. 4 (1), 1151(2021).</li>
<li>White, B. M., Kumar, P., Conwell, A. N., Wu, K., Baskin, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lipid+expansion+microscopy%5BTitle%5D)+AND+%22Journal+of+the+American+Chemical+Society%22%5BJournal%5D)">Lipid expansion microscopy.</a> Journal of the American Chemical Society. 144 (40), 18212-18217 (2022).</li>
<li>Steib, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((TissUExM+enables+quantitative+ultrastructural+analysis+in+whole+vertebrate+embryos+by+expansion+microscopy%5BTitle%5D)+AND+%22Cell+Reports+Methods%22%5BJournal%5D)">TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopy.</a> Cell Reports Methods. 2 (10), 100311(2022).</li>
<li>Shaib, A. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Visualizing+proteins+by+expansion+microscopy%5BTitle%5D)+AND+%22bioRxiv%22%5BJournal%5D)">Visualizing proteins by expansion microscopy.</a> bioRxiv. , (2023).</li>
<li>M'Saad, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((All-optical+visualization+of+specific+molecules+in+the+ultrastructural+context+of+brain+tissue%5BTitle%5D)+AND+%22bioRxiv%22%5BJournal%5D)">All-optical visualization of specific molecules in the ultrastructural context of brain tissue.</a> bioRxiv. , (2022).</li>
</ol>

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ultrastructurele expansiemicroscopie in drie in vitro levenscyclusstadia van trypanosoma cruzi

Ultrastructural Expansion Microscopy in Three In Vitro Life Cycle Stages of Trypanosoma cruzi

Ultrastructurele expansiemicroscopie in drie in vitro levenscyclusstadia van Trypanosoma cruzi

We describe here the application of ultrastructure expansion microscopy (U-ExM) in Trypanosoma cruzi, a technique that allows increasing the spatial ...

ultrastructural-expansion-microscopy-three-vitro-life-cycle-stages

Research

JoVE Journal

Biology

Biologie

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Leer ultrastructuur-expansiemicroscopie toe te passen op drie in vitro levenscyclusstadia van Trypanosoma cruzi. Deze methode maakt nanoschaalbeeldvorming mogelijk met standaard microscopen voor gedetailleerde 3D-reconstructies van cellulaire structuren.

Ultrastructurele expansiemicroscopie in drie in vitro levenscyclusstadia van Trypanosoma cruzi

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