Name: Multi-tracerstudies van het zuurstof- en glucosemetabolisme in de hersenen met behulp van een time-of-flight positronemissietomografie-computertomografiescanner (Video) | JoVE Journal
Uploaded: 2024-06-07T12:30:00.000+00:00
Duration: 8 min 36 s
Description: Leer hoe je hersenmetabolisme kwantificeert met PET-CT voor onderzoek naar veroudering en neurodegeneratieve ziekten. Dit protocol meet glucose- en zuurstofverbruik in vivo bij menselijke proefpersonen.

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<meta charset="utf8"></head><body>PET-CT-scanning voor multi-traceronderzoek van het cerebrale zuurstof- en glucosemetabolisme

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</ol>

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multi-tracerstudies van het zuurstof- en glucosemetabolisme in de hersenen met behulp van een time-of-flight positronemissietomografie-computertomografiescanner

Multi-Tracer Studies of Brain Oxygen and Glucose Metabolism Using a Time-of-Flight Positron Emission Tomography-Computed Tomography Scanner

Multi-tracerstudies van het zuurstof- en glucosemetabolisme in de hersenen met behulp van een time-of-flight positronemissietomografie-computertomografiescanner

The authors have developed a paradigm using positron emission tomography (PET) with multiple radiopharmaceutical tracers that combines measurements of ...

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Source: Jamadar, S. et. al. Radiotracer Administration for High Temporal Resolution Positron Emission Tomography of the Human Brain: Application to FDG-fPET. J. Vis. Exp. (2019)This video demonstrates the quantification of brain glucose metabolism using positron emission tomography (PET). The participant is infused with the tracer F-18 fluorodeoxyglucose (FDG) during scanning. FDG accumulates in active neurons and emits positrons upon F-18 decay. These positrons interact with electrons,...

Positron Emission Tomography Imaging of the Human Brain Using a Radiotracer

Positron-emissietomografie voor kleine dieren maakt de beoordeling mogelijk van de twee belangrijkste energiesubstraten van de hersenen: glucose en ketonen. In de huidige methode worden 11C-acetoacetaat en 18F-fluorodeoxyglucose sequentieel in elk dier geïnjecteerd, en hun opname wordt kwantitatief gemeten in specifieke hersengebieden die zijn bepaald op basis van de magnetische resonantiebeelden.

Een dubbel Tracer PET-MRI Protocol voor de kwantitatieve meting van regionale opname van hersenenergiesubstraten bij de rat

[18F]-fluorodeoxyglucose (FDG) positron emissie tomografie-berekend tomografie is handig voor het glucose metabolisme aan hersenfunctie gerelateerde studie. Hier presenteren we een protocol voor een [18F] FDG tracer set-up en semikwantitatieve beoordeling van de regio-of-interest-analyse voor gerichte hersengebieden klinische verschijnselen bij patiënten met ernstig traumatisch hersenletsel is gekoppeld.

Semi-kwantitatieve beoordeling [18F] FDG Tracer gebruiken bij patiënten met ernstige hersenletsel

Dit manuscript beschrijft twee radiotracer beheerprotocollen voor FDG-PET (constante infusie en bolus plus infusie) en vergelijkt deze met bolustoediening. Tijdelijke resoluties van 16 s zijn haalbaar met behulp van deze protocollen.

Radiotracer toediening voor hoge temporele resolutie positron emissie tomografie van het menselijk brein: toepassing op FDG-fPET

De verwerving van dynamische positronemissietomografie (PET)-gegevens en reconstructie in tijdsbestekken maakt analyse van metabole hersenconnectiviteit op het niveau van één persoon mogelijk. We beschrijven een methode om [18F]FDG dynamische PET-gegevens van de hersenen van de rat te verkrijgen en een connectiviteitsmatrix te verkrijgen door de extractie van tijd-activiteitscurven van interessante volumes.

Studie van metabole hersenconnectiviteit met behulp van 2-Deoxy-2-[18F]Fluoro-D-Glucose dynamische positronemissietomografie op het niveau van één proefpersoon

We presenteren een experimentele procedure voor het meten van de partiële druk van zuurstof (pO2) in cerebrale vaatstelsel op basis van zuurstof-afhankelijke quenching van fosforescentie. Dier voorbereiding en beeldvormende procedures werden uiteengezet voor zowel grote beeldveld van CCD-gebaseerde beeldvorming van pO2 in ratten en twee-foton excitatie gebaseerde beeldvorming van de pO2 in muizen.

Cerebral Blood Oxygenatie meting op basis van zuurstof-afhankelijke Afschrikken van Fosforescentie

Deze methode beschrijft de generatie van organotypische cerebellaire culturen en het effect van bepaalde apoptotische stimuli op de levensvatbaarheid van de verschillende cerebellaire celtypen.

Organotypische Cerebellaire culturen: apoptotische uitdagingen en Detectie

Hier wordt een procedure gepresenteerd om selectief een neuronaal eiwit te activeren met een korte lichtpuls door genetisch een foto-reactieve onnatuurlijke aminozuur te coderen in een doelneuronaal eiwit dat tot expressie wordt gebracht in neuronen in cultuur of in vivo.

Optische controle van een neuronaal proteïne met behulp van een genetisch gecodeerde Onnatuurlijke aminozuren in neuronen

Hier presenteren we een protocol om een genetisch gelabelde subpopulatie van neuronen door een twee-foton laser vanaf Zebrafish larven lasertherapie.

Ablatie van een neuronale bevolking met behulp van een twee-foton Laser en haar beoordeling met Calcium Imaging en gedrags opname in Zebrafish larven

Dit document beschrijft een nieuwe model van traumatische hersenenverwonding primaire blast. Een drukluchtremmen gedreven schok buis wordt gebruikt voor het blootstellen van in vitro hippocampal segment culturen van de muis om een enkele schokgolf. Dit is een eenvoudige en snelle protocol voor het genereren van een reproduceerbare hersenletsel met weefsel met een hoog debiet.

Een roman In Vitro Model van traumatische hersenenverwonding Blast

Leer hoe je hersenmetabolisme kwantificeert met PET-CT voor onderzoek naar veroudering en neurodegeneratieve ziekten. Dit protocol meet glucose- en zuurstofverbruik in vivo bij menselijke proefpersonen.

Multi-tracerstudies van het zuurstof- en glucosemetabolisme in de hersenen met behulp van een time-of-flight positronemissietomografie-computertomografiescanner (Video) | JoVE Journal

Multi-tracerstudies van het zuurstof- en glucosemetabolisme in de hersenen met behulp van een time-of-flight positronemissietomografie-computertomografiescanner

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