Name: Fentanyl analoge screening met behulp van LC-TIMS-TOF MS/MS (Video) | JoVE Journal
Uploaded: 2024-11-08T12:00:00.000+00:00
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Description: Leer een LC-TIMS-TOF MS/MS-methode om isomere fentanyl-analogen te onderscheiden met vier orthogonale karakteriseringstechnieken. Deze aanpak verbetert de identificatie van structurele isomeren voor drugshandhaving.

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<meta charset="utf8"></head><body>Het oogsten van de orthogonaliteit van LC en TIMS-TOF MS/MS om fentanyl-analogen te screenen

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</ol>

<table><tbody><tr><td>Ammonium formate for HPLC</td><td>Fluka</td><td>17843-50G</td><td></td></tr><tr><td>Eppendorf&nbsp;Snap-Cap Microcentrifuge Safe-Lock Tubes</td><td>Fisher</td><td>05-402-25</td><td></td></tr><tr><td>ESI-L Low Concentration Tuning mix</td><td>Agilent</td><td>G1969-85000</td><td></td></tr><tr><td>Fentanyl Analog Screening (FAS) Kit&nbsp;</td><td>Cayman Chemical</td><td>9003237, 9003286, 9003380, 9003381</td><td>kit van 250 synthetische opioïden, 210 fentanyl analogen, verdeeld in een kit en emergente panelversies 1-4</td></tr><tr><td>Formic acid Optima LC/MS</td><td>Fisher</td><td>A117-50</td><td></td></tr><tr><td>Onyx guard column (5 x 4.6 mm)&nbsp;</td><td>Phenomenex</td><td>CHO-7649</td><td>guard column voor C18 kolommen</td></tr><tr><td>Onyx monolithic C18 HPLC column (100 x 4.6 mm)</td><td>Phenomenex</td><td>CHO-7643</td><td>reverse phase C18 LC kolom</td></tr><tr><td>Optima grade acetonitrile&nbsp;</td><td>Fisher</td><td>A996-4</td><td></td></tr><tr><td>Optima grade methanol</td><td>Fisher</td><td>A454-4</td><td></td></tr><tr><td>Optima grade water</td><td>Fisher</td><td>W7-4</td><td></td></tr><tr><td>Pipette</td><td>Fisher</td><td>05-719-510</td><td>kit van 1-10 &micro;L, 10-100 &micro;L, en 100-1000 &micro;L pipette</td></tr><tr><td>Pipette tips 10&micro;L</td><td>Fisher</td><td>94060100</td><td></td></tr><tr><td>Pipette tips 1000&micro;L</td><td>Fisher</td><td>94056710</td><td></td></tr><tr><td>Pipette tips 200&micro;L</td><td>Fisher</td><td>94060310</td><td></td></tr><tr><td>Plate mixer</td><td>IKA&nbsp;</td><td>MS 3 D S1</td><td>IKA MS 3 digitaal</td></tr><tr><td>Prominence LC-20 CE ultrafast liquid chromatograph</td><td>Shimadzu, Japan</td><td></td><td>uitgerust met DGU-20A5, LC-20AD, SIL-20AC, CTO-20A, SPD-M20A, CBM-20A, SPD-20A</td></tr><tr><td>timsTOF Pro</td><td>Bruker Daltonics Inc., Billerica, MA</td><td></td><td>timsTOF instrument met PASEF&nbsp;</td></tr></tbody></table>

fentanyl analoge screening met behulp van lc-tims-tof ms/ms

Fentanyl Analog Screening using LC-TIMS-TOF MS/MS

Fentanyl analoge screening met behulp van LC-TIMS-TOF MS/MS

The use of fentanyl and the emergence of fentanyl analogs over recent decades has become an increasing concern to the community at large. Fentanyl and its ...

fentanyl-analog-screening-using-lc-tims-tof-msms

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Een analytische workflow op basis van vloeistofchromatografie, mobiliteitsspectrometrie met ingesloten ionen en time-of-flight-massaspectrometrie (LC-TIMS-ToF MS/MS) voor een zeer betrouwbare en zeer reproduceerbare "bottom-up"-analyse van histonmodificaties en identificatie op basis van de belangrijkste parameters (retentietijd [RT], botsingsdoorsnede [CCS] en nauwkeurige massa-tot-lading [m/z]-verhouding).

Screening op histonmodificatie met behulp van vloeistofchromatografie, Trapped Ion Mobility Spectrometry en Time-Of-Flight Mass Spectrometry

We presenteren een methode voor snelle massaspectrometrie (MS)/massaspectrometrie (MS)-gebaseerde annotatie en classificatie van tropaanalkaloïden, nuttig voor zowel voorlopige dereplicatie van tropane-bevattende monsters als de ontdekking van nieuwe alkaloïden voor isolatie.

Toepassingen van vloeistofchromatografie tandem massaspectrometrie in onderzoek naar natuurlijke producten: tropaanalkaloïden als casestudy

Dit protocol presenteert een gevalideerde massaspectrometriemethode voor vloeistofchromatografie-ionenmobiliteit en hoge resolutie om de aanwezigheid van moederkorenalkaloïden in voedsel te bepalen in overeenstemming met de onlangs uitgebrachte Verordening (EU) 2023/915 van de Commissie.

Detectie van gereguleerde moederkorenalkaloïden in voedselmatrices door middel van vloeistofchromatografie-ingesloten ionenmobiliteitsspectrometrie-time-of-flight massaspectrometrie

Een begeleide materiaal screening benadering van sol-gel afkomstig eiwit gedoteerde microarrays met behulp van een opkomende methode pin-drukken van fabricage ontwikkeling wordt beschreven. Deze methode wordt aangetoond door de ontwikkeling van acetylcholinesterase en multikinaseremmer microarrays, die voor rendabele kleine moleculen screening.

A Guided Materialen Screening aanpak voor de ontwikkeling van kwantitatieve Sol-gel Afgeleid Eiwit Microarrays

Leer een LC-TIMS-TOF MS/MS-methode om isomere fentanyl-analogen te onderscheiden met vier orthogonale karakteriseringstechnieken. Deze aanpak verbetert de identificatie van structurele isomeren voor drugshandhaving.

Fentanyl analoge screening met behulp van LC-TIMS-TOF MS/MS

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