Name: Dioscine gemedieerde verlichting van IgA-nefropathie door B-celactivering in vivo te remmen en de galactose-deficiënte IgA1-productie in vitro te verminderen (Video) | JoVE Journal
Uploaded: 2023-10-13T14:40:00.000+00:00
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Description: Ontdek hoe dioscine IgA-nefropathie behandelt door B-celactivatie in muismodellen te remmen en galactose-deficiënte IgA1-productie in DAKIKI-cellen te verminderen.

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<meta charset="utf8"></head><body>Immunoglobuline A-nefropathie (IgAN) behandelen met dioscine in een muizenmodel

<ol><li>Knoppova, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+origin+and+activities+of+IgA1-containing+immune+complexes+in+IgA+nephropathy%5BTitle%5D)+AND+%22Frontiers+in+Immunology%22%5BJournal%5D)">The origin and activities of IgA1-containing immune complexes in IgA nephropathy.</a> Frontiers in Immunology. 7, 117(2016).</li>
<li>Suzuki, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+pathophysiology+of+IgA+nephropathy%5BTitle%5D)+AND+%22Journal+of+The+American+Society+of+Nephrology%22%5BJournal%5D)">The pathophysiology of IgA nephropathy.</a> Journal of The American Society of Nephrology. 22 (10), 1795-1803 (2011).</li>
<li>He, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Synthetic+double-stranded+RNA+poly%28I%3AC%29+aggravates+IgA+nephropathy+by+triggering+IgA+class+switching+recombination+through+the+TLR3-BAFF+axis%5BTitle%5D)+AND+%22American+Journal+of+Nephrology%22%5BJournal%5D)">Synthetic double-stranded RNA poly(I:C) aggravates IgA nephropathy by triggering IgA class switching recombination through the TLR3-BAFF axis.</a> American Journal of Nephrology. 42 (3), 185-197 (2015).</li>
<li>Zhao, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+level+of+galactose-deficient+IgA1+in+the+sera+of+patients+with+IgA+nephropathy+is+associated+with+disease+progression%5BTitle%5D)+AND+%22Kidney+International%22%5BJournal%5D)">The level of galactose-deficient IgA1 in the sera of patients with IgA nephropathy is associated with disease progression.</a> Kidney International. 82 (7), 790-796 (2012).</li>
<li>Xing, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((C1GALT1+expression+is+associated+with+galactosylation+of+IgA1+in+peripheral+B+lymphocyte+in+immunoglobulin+a+nephropathy%5BTitle%5D)+AND+%22BMC+Nephrology%22%5BJournal%5D)">C1GALT1 expression is associated with galactosylation of IgA1 in peripheral B lymphocyte in immunoglobulin a nephropathy.</a> BMC Nephrology. 21 (1), 18(2020).</li>
<li>Qin, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((External+suppression+causes+the+low+expression+of+the+Cosmc+gene+in+IgA+nephropathy%5BTitle%5D)+AND+%22Nephrology+Dialysis+Transplantation%22%5BJournal%5D)">External suppression causes the low expression of the Cosmc gene in IgA nephropathy.</a> Nephrology Dialysis Transplantation. 23 (5), 1608-1614 (2008).</li>
<li>Sakai, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lactobacillus+gasseri+SBT2055+induces+TGF-%CE%B2+expression+in+dendritic+cells+and+activates+TLR2+signal+to+produce+IgA+in+the+small+intestine%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Lactobacillus gasseri SBT2055 induces TGF-β expression in dendritic cells and activates TLR2 signal to produce IgA in the small intestine.</a> PLoS One. 9 (8), 105370(2014).</li>
<li>Gutzeit, C., Magri, G., Cerutti, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intestinal+IgA+production+and+its+role+in+host-microbe+interaction%5BTitle%5D)+AND+%22Immunological+Reviews%22%5BJournal%5D)">Intestinal IgA production and its role in host-microbe interaction.</a> Immunological Reviews. 260 (1), 76-85 (2014).</li>
<li>Serino, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+a+retrospective+international+study%2C+circulating+miR-148b+and+let-7b+were+found+to+be+serum+markers+for+detecting+primary+IgA+nephropathy%5BTitle%5D)+AND+%22Kidney+International%22%5BJournal%5D)">In a retrospective international study, circulating miR-148b and let-7b were found to be serum markers for detecting primary IgA nephropathy.</a> Kidney International. 89 (3), 683-692 (2016).</li>
<li>Lu, F., et al. Therapeutic effect of Rhizoma Dioscoreae Nipponicae on gouty arthritis based on the SDF-1/CXCR 4 and p38 MAPK pathway: an in vivo and in vitro study. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aLu%2C+F+%22Phytotherapy+research%3A+PTR%22">Phytotherapy research: PTR</a>. 28 (2), 280-288 (2014).</li>
<li>Wang, W., Xu, L., Zhou, L., Wan, S., Jiang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+Network+pharmacology+approach+to+reveal+the+underlying+mechanisms+of+Rhizoma+Dioscoreae+Nipponicae+in+the+treatment+of+asthma%5BTitle%5D)+AND+%22Evidence-Based+Complementary+and+Alternative+Medicine%3A+eCAM%22%5BJournal%5D)">A Network pharmacology approach to reveal the underlying mechanisms of Rhizoma Dioscoreae Nipponicae in the treatment of asthma.</a> Evidence-Based Complementary and Alternative Medicine: eCAM. 2022, 4749613(2022).</li>
<li>Tian, W. W., Wei, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Professor+TONG+Xiaolin+used+the+experience+of+Dioscoreae+Nipponicae%5BTitle%5D)+AND+%22Jilin+Journal+of+Chinese+Medicine%22%5BJournal%5D)">Professor TONG Xiaolin used the experience of Dioscoreae Nipponicae.</a> Jilin Journal of Chinese Medicine. 40 (05), 589-592 (2020).</li>
<li>Rao, X. R., Bai, Y. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Das+Xiwen%27s+experience+in+treating+IgA+nephropathy%5BTitle%5D)+AND+%22Beijing+Journal+of+Traditional+Chinese+Medicine%22%5BJournal%5D)">Das Xiwen's experience in treating IgA nephropathy.</a> Beijing Journal of Traditional Chinese Medicine. 9, 691-693 (2008).</li>
<li>Si, Y., Zhang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+data+mining+study+on+the+pattern+of+medication+use+in+the+treatment+of+IgA+nephropathy+by+Professor+Zhang+Yu%5BTitle%5D)+AND+%22Journal+of+Chinese+Physician%22%5BJournal%5D)">A data mining study on the pattern of medication use in the treatment of IgA nephropathy by Professor Zhang Yu.</a> Journal of Chinese Physician. 20 (01), 109-111 (2018).</li>
<li>Jiang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+the+enzymatic+extraction+technology+of+Diosgenin+from+Dioscorea+nipponica%5BTitle%5D)+AND+%22Chinese+Traditional+Patent+Medicine%22%5BJournal%5D)">Optimization of the enzymatic extraction technology of Diosgenin from Dioscorea nipponica.</a> Chinese Traditional Patent Medicine. 39 (03), 621-624 (2017).</li>
<li>Qi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dioscin+alleviates+lipopolysaccharide-induced+inflammatory+kidney+injury+via+the+microRNA+let-7i%2FTLR4%2FMyD88+signaling+pathway%5BTitle%5D)+AND+%22Pharmacological+Research%22%5BJournal%5D)">Dioscin alleviates lipopolysaccharide-induced inflammatory kidney injury via the microRNA let-7i/TLR4/MyD88 signaling pathway.</a> Pharmacological Research. 111, 509-522 (2016).</li>
<li>Yang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+advances+in+the+pharmacological+activities+of+Dioscin%5BTitle%5D)+AND+%22BioMed+Research+International%22%5BJournal%5D)">Recent advances in the pharmacological activities of Dioscin.</a> BioMed Research International. 2019, 5763602(2019).</li>
<li>Nal Zou, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Toll-like+receptor+4+signaling+pathway+in+the+protective+effect+of+Pioglitazone+on+experimental+immunoglobulin+A+nephropathy%5BTitle%5D)+AND+%22Chinese+Medical+Journal%22%5BJournal%5D)">Toll-like receptor 4 signaling pathway in the protective effect of Pioglitazone on experimental immunoglobulin A nephropathy.</a> Chinese Medical Journal. 130 (8), 906-913 (2017).</li>
<li>Xu, S. Y., Bian, R. L., Chen, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pharmacological+experiments+methodology%5BTitle%5D)+AND+%22Chinese+Pharmacological+Bulletin%22%5BJournal%5D)">Pharmacological experiments methodology.</a> Chinese Pharmacological Bulletin. 1, 19(1992).</li>
<li>Shen, J. C., Ren, Y., Rao, X. R., You, Y., Li, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Network+pharmacology%2C+molecular+docking%2C+and+in+vitro+experiments+to+explore+the+molecular+mechanism+of+Dioscorea+Nipponica+Makion+in+the+treatment+of+IgA+nephropathy%5BTitle%5D)+AND+%22World+Journal+of+Integrated+Traditional+and+Western+Medicine%22%5BJournal%5D)">Network pharmacology, molecular docking, and in vitro experiments to explore the molecular mechanism of Dioscorea Nipponica Makion in the treatment of IgA nephropathy.</a> World Journal of Integrated Traditional and Western Medicine. 16 (12), 2246-2254 (2021).</li>
<li>Mestecky, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((IgA+nephropathy%3A+molecular+mechanisms+of+the+disease%5BTitle%5D)+AND+%22Annual+Review+of+Pathology%22%5BJournal%5D)">IgA nephropathy: molecular mechanisms of the disease.</a> Annual Review of Pathology. 8, 217-240 (2013).</li>
<li>Novak, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((IgA1-containing+immune+complexes+in+IgA+nephropathy+differentially+affect+proliferation+of+mesangial+cells%5BTitle%5D)+AND+%22Kidney+International%22%5BJournal%5D)">IgA1-containing immune complexes in IgA nephropathy differentially affect proliferation of mesangial cells.</a> Kidney International. 67 (2), 504-513 (2005).</li>
<li>Nihei, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Identification+of+IgA+autoantibodies+targeting+mesangial+cells+redefines+the+pathogenesis+of+IgA+nephropathy%5BTitle%5D)+AND+%22Science+Advances%22%5BJournal%5D)">Identification of IgA autoantibodies targeting mesangial cells redefines the pathogenesis of IgA nephropathy.</a> Science Advances. 9 (12), (2023).</li>
<li>Raska, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Identification+and+characterization+of+CMP-NeuAc%3A+GalNAc-IgA1+alpha2%2C6-sialyltransferase+in+IgA1-producing+cells%5BTitle%5D)+AND+%22Journal+of+Molecular+Biology%22%5BJournal%5D)">Identification and characterization of CMP-NeuAc: GalNAc-IgA1 alpha2,6-sialyltransferase in IgA1-producing cells.</a> Journal of Molecular Biology. 369 (1), 69-78 (2007).</li>
</ol>

<table><tbody><tr><td>Anti-CD20/MS4A1 Antibody</td><td>Boster Biotechnology Company</td><td>A03780-3</td><td></td></tr><tr><td>Antifade mounting medium</td><td>Beyotime, Shanghai, China</td><td>P0128S</td><td></td></tr><tr><td>Balb/c mice</td><td>Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd.</td><td>110322220101424000</td><td></td></tr><tr><td>blocking serum</td><td>Solarbio, Beijing, China</td><td>SL038</td><td></td></tr><tr><td>Bovine gamma globulin</td><td>ShangHai YuanYe Biotechnology Company</td><td>S12031</td><td></td></tr><tr><td>C1GALT1 polyclonal antibody</td><td>Proteintech Group, Inc,USA</td><td>27569-1-AP</td><td></td></tr><tr><td>Citrate antigen retrieval solution(50&times;)</td><td>Phygene Biotechnology Company</td><td>PH0422</td><td></td></tr><tr><td>COSMC polyclonal antibody</td><td>Proteintech Group, Inc,USA</td><td>19254-1-AP</td><td></td></tr><tr><td>Cytotoxiciy detection kit</td><td>Roche Company</td><td>4744926001</td><td></td></tr><tr><td>Dako REAL EnVision detection system, Peroxidase/DAB+</td><td>Dako</td><td>K5007</td><td></td></tr><tr><td>DAPI</td><td>Invitrogen</td><td>D1306</td><td></td></tr><tr><td>Dioscin</td><td>National Institute For Food and Drug Control</td><td>111707-201703</td><td></td></tr><tr><td>DIO tablets</td><td>Chengdu No 1 Pharmaceutical Co. Ltd.</td><td>H51023866</td><td></td></tr><tr><td>ECL working solution</td><td>Merck Biotechnology, Inc</td><td>WBKLS0100&nbsp;</td><td></td></tr><tr><td>Enhanced cell counting kit-8</td><td>Beyotime, Shanghai, China</td><td>C0043</td><td></td></tr><tr><td>Fasking one-step removal of gene cDNA first-strand synthesis premix</td><td>TIANGEN,Beijing, China</td><td>KR118-02</td><td></td></tr><tr><td>Glycogen Periodic acid Schiff (PAS) stain kit&nbsp;</td><td>BaSO Biotechnology Company</td><td>BA4080A</td><td></td></tr><tr><td>Goat anti-mouse IgA-AF488</td><td>SouthernBiotech</td><td>1040-30</td><td></td></tr><tr><td>Goat anti-rabbit IgG antibody (H+L), HRP conjugated</td><td>BeiJing Bioss Biotechnology Company</td><td>BS-0295G-HRP</td><td></td></tr><tr><td>Human Gd-IgA1 ELISA kit</td><td>IBL</td><td>27600</td><td></td></tr><tr><td>Human IgA ELISA kit</td><td>MultiSciences (LiankeBio)</td><td>70-EK174-96</td><td></td></tr><tr><td>Pierce BCA protein assay kit</td><td>Thermo Scientific</td><td>23227</td><td></td></tr><tr><td>PMSF solution</td><td>Beyotime, Shanghai, China</td><td>ST507</td><td></td></tr><tr><td>Proteinase K&nbsp;</td><td>Phygene Biotechnology Company</td><td>PH1521</td><td></td></tr><tr><td>Rabbit anti-CXCR5 polyclonal antibody&nbsp;</td><td>BeiJing Bioss Biotechnology Company</td><td>bs-23570R</td><td></td></tr><tr><td>RIPA lysis buffer</td><td>Beyotime, Shanghai, China</td><td>P0013B</td><td></td></tr><tr><td>RNAsimple total RNA extraction kit</td><td>TIANGEN,Beijing, China</td><td>DP419</td><td></td></tr><tr><td>RPMI Medium 1640</td><td>Solarbio, Beijing, China</td><td>31800</td><td></td></tr><tr><td>Super-Bradford protein assay kit</td><td>CWBIO, Beijing, China</td><td>CW0013</td><td></td></tr><tr><td>Triton&nbsp;X-100</td><td>Beyotime, Shanghai, China</td><td>ST795</td><td></td></tr><tr><td>&beta;-Actin Rabbit mAb</td><td>Abclonal, Wuhan, China</td><td>AC026</td><td></td></tr></tbody></table>

dioscine gemedieerde verlichting van iga-nefropathie door b-celactivering in vivo te remmen en de galactose-deficiënte iga1-productie in vitro te verminderen

Dioscin Mediated IgA Nephropathy Alleviation by Inhibiting B Cell Activation In Vivo and Decreasing Galactose-Deficient IgA1 Production In Vitro

Dioscine gemedieerde verlichting van IgA-nefropathie door B-celactivering in vivo te remmen en de galactose-deficiënte IgA1-productie in vitro te verminderen

The increase of circulating galactose-deficient IgA1 (Gd-IgA1) is caused by excessive activation of IgA-positive secretory cells in the process of mucosal ...

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Ontdek hoe dioscine IgA-nefropathie behandelt door B-celactivatie in muismodellen te remmen en galactose-deficiënte IgA1-productie in DAKIKI-cellen te verminderen.

Dioscine gemedieerde verlichting van IgA-nefropathie door B-celactivering in vivo te remmen en de galactose-deficiënte IgA1-productie in vitro te verminderen (Video) | JoVE Journal

Dioscine gemedieerde verlichting van IgA-nefropathie door B-celactivering in vivo te remmen en de galactose-deficiënte IgA1-productie in vitro te verminderen

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