Name: Een hulpmiddel om automatisch stabiele en reproduceerbare celvrije openingen te creëren voor het verbeteren van de betrouwbaarheid van de celwondgenezingstest (Video) | JoVE Journal
Uploaded: 2024-10-04T12:30:00.000+00:00
Duration: 6 min 13 s
Description: Leer een innovatieve celscraper voor celwondgenezingstests die reproduceerbaarheid en betrouwbaarheid verbetert. Deze methode behoudt celintegriteit en verhoogt overlevings- en proliferatiepercentages.

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<meta charset="utf8"></head><body>Innoveren van een verbeterde celschraper voor celbeschadiging door analyse van gebruikersvereisten

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</ol>

<table><tbody><tr><td>CCK-8 Kit</td><td>Beyotime Company, China</td><td>C0037</td><td></td></tr><tr><td>digitaal microscoopsysteem</td><td>Olympus</td><td>IX81</td><td></td></tr><tr><td>foetaal bovien serum</td><td>Gibco, USA</td><td>16000044</td><td></td></tr><tr><td>HOS&nbsp;</td><td>Procell Life Science &amp; Technology Co., Ltd</td><td>CL-0360</td><td></td></tr><tr><td>Image-Pro Plus</td><td>Media Cybernetics</td><td>versie 6.0</td><td></td></tr><tr><td>KeyShot</td><td>Luxion</td><td>versie 11.0</td><td>3D rendersoftware</td></tr><tr><td>microplatelezer</td><td>BioTek, Duitsland</td><td>ELX808</td><td></td></tr><tr><td>Minimum Essential Medium</td><td>Gibco, USA</td><td>11095080</td><td></td></tr><tr><td>Pantone-matchsysteem</td><td>Pantone</td><td></td><td>commercieel kleurmatchsysteem</td></tr><tr><td>penicilline-streptomycine</td><td>Beyotime Company, China</td><td>ST488</td><td></td></tr><tr><td>Photoshop</td><td>Adobe</td><td></td><td>foto- en ontwerpsoftware</td></tr><tr><td>Rhinoceros 3D</td><td>Robert McNeel &amp; Associates</td><td>versie 7.0</td><td>3D ontwerpsoftware</td></tr><tr><td>TC20 geautomatiseerde celteller</td><td>Bio-Rad</td><td>TC20</td><td></td></tr><tr><td>Trypsine</td><td>Cytiva&nbsp;HyClone, Verenigde Staten</td><td>SH30042.01</td><td></td></tr></tbody></table>

een hulpmiddel om automatisch stabiele en reproduceerbare celvrije openingen te creëren voor het verbeteren van de betrouwbaarheid van de celwondgenezingstest

A Tool to Automatically Create Stable and Reproducible Cell-free Gaps for Improving the Reliability of Cell Wound Healing Assay

Een hulpmiddel om automatisch stabiele en reproduceerbare celvrije openingen te creëren voor het verbeteren van de betrouwbaarheid van de celwondgenezingstest

The reliability of cellular migration measurement in wound healing assays is frequently undermined by the prevalent methodological instability, i.e., ...

a-tool-to-automatically-create-stable-reproducible-cell-free-gaps-for

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In vitro cel-migratie en invasie assays

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Hier presenteren we een protocol bij een semi-high throughput celmigratie analyse uit te voeren op een 96-well celcultuur plaat. Dit protocol is een snelle, eenvoudige en economische methode om consistente kras wonden op een cel monolaag te creëren.

Een inrichting voor het uitvoeren van celmigratie / wondgenezing in een 96-putjesplaat

Dit manuscript beschrijft hoe incisie-achtige letsels gemaakt op gekweekte epitheliale cel monolayers gunstig model wond genezing in vitro, waardoor voor beeldvorming door confocal of laser scanning microscopie, en die kan voorzien van kwalitatief hoogwaardige kwantitatieve en kwalitatieve gegevens voor het bestuderen van zowel cel gedrag en de mechanismen die betrokken zijn bij migratie.

Methoden voor de beoordeling van epitheliale cel migratie tijdens het In Vitro wondgenezing op basis van microscopie

Hier presenteren we een protocol om te evalueren van het effect van peptiden op de migratie van bronchiale epitheliale cellen. Deze methode zorgt voor de snelle en zeer reproduceerbaar accumulatie van kwantitatieve gegevens over de snelheid van cel migratie en wond sluiting.

Een roman In Vitro wond genezing Assay om te evalueren van de cel migratie

Hier beschrijven we een procedure om te testen de cel migratie in vitro met een geautomatiseerde optische camera Microscoop. De kras assay is wijd verbeid gebruikt wanneer de sluiting van de kras gedurende een ingestelde periode wordt gevolgd. De optische Microscoop kunt betrouwbare en goedkope detectie van cel migratie.

Kras Assay voor In Vitro testen van cel migratie met een geautomatiseerde optische Camera geoptimaliseerd

Het huidige protocol beschrijft een geïntegreerde methode onderzoek naar kanker cel migratie en invasie op één platform in real-time, het verstrekken van een gemakkelijk reproduceerbaar zijn en tijd-efficiënte optie te bestuderen van de cel mobiliteit en morfologie.

Een eenvoudige migratie/invasie Workflow met behulp van een geautomatiseerde Live-cel Imager

Hier presenteren we een protocol voor een in vitro Scratch assay met primaire fibroblasten en voor een in vivo huid wond genezings test bij muizen. Beide assays zijn eenvoudige methoden om te beoordelen in vitro en in vivo wondgenezing.

Scratch Migration assay en dorsale skinfold Chamber voor in vitro en in vivo analyse van wondgenezing

Deze studie presenteert twee cruciale experimentele technieken: de constructie van een muiswondmodel voor het beoordelen van wondsluiting en de voorbereiding van suspensies van enkele cellen uit muishuid om de celviabiliteit te behouden.

Muizenwondmodellen en het bereiden van single-cell suspensies

Leer een innovatieve celscraper voor celwondgenezingstests die reproduceerbaarheid en betrouwbaarheid verbetert. Deze methode behoudt celintegriteit en verhoogt overlevings- en proliferatiepercentages.

Een hulpmiddel om automatisch stabiele en reproduceerbare celvrije openingen te creëren voor het verbeteren van de betrouwbaarheid van de celwondgenezingstest (Video) | JoVE Journal

Een hulpmiddel om automatisch stabiele en reproduceerbare celvrije openingen te creëren voor het verbeteren van de betrouwbaarheid van de celwondgenezingstest

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