Name: Beoordeling van de connectiviteitsuitkomstmaten van de motoreenheid van het rattendiafragma als kwantitatieve biomarkers van degeneratie en compensatie van phrenische motorneuronen (Video) | JoVE Journal
Uploaded: 2024-04-19T14:10:00.000+00:00
Duration: 6 min 8 s
Description: Leer de MuNI-techniek voor het kwantificeren van motorische eenheden in het rattenmiddenrif. Deze niet-invasieve methode maakt longitudinale beoordeling van neuromusculaire functie mogelijk.

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<meta charset="utf8"></head><body>Kwantificering van de functie van de phrenicum motoreenheid in knaagdiermodellen van neurodegeneratie

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<table><tbody><tr><td>2 mL Glass Syringe</td><td>Kent Scientific Corporation</td><td>SOMNO-2ML</td><td></td></tr><tr><td>50 mL, Model 705 RN-slangpomp</td><td>Hamilton Company</td><td>7637-01</td><td>Gebruikt voor intrapleurale injectie</td></tr><tr><td>5008 - Formulab Dieet</td><td>LabDiet</td><td>0001325</td><td></td></tr><tr><td>Autoclaveerbare 26 G naalden (26S RN 9,52 mm 40&deg;)</td><td>Hamilton Company</td><td>7804-04</td><td>Gebruikt voor intrapleurale injectie</td></tr><tr><td>Cholera toxine B-subunit (CTB)</td><td>MilliporeSigma</td><td>C9903</td><td>Gebruikt voor intrapleurale injectie om overlevende motorneuronen te labelen</td></tr><tr><td>Cholera toxine B-subunit geconjugeerd aan saporine (CTB-SAP)</td><td>Advanced Targeting Systems</td><td>IT-14</td><td>Gebruikt voor intrapleurale injectie om motorneurondood te veroorzaken</td></tr><tr><td>Afneembare kabel</td><td>Technomed</td><td>202845-0000</td><td>om de recorderelektrode op de elektrodiagnostische machine aan te sluiten</td></tr><tr><td>Wegneembare 2" x 2" schijfelektrode met aansluitdraden</td><td>Cadwell</td><td>302290-000</td><td>aardelektrode</td></tr><tr><td>Wegneembare monopolare naalden 28 G</td><td>Technomed</td><td>202270-000</td><td>kathodische en anodisch stimulerende elektroden - registratie-elektroden</td></tr><tr><td>EMG-naaldenkabel (Amp/stim-schakelbox)</td><td>Cadwell</td><td>190266-200</td><td>om monopolaire elektroden op elektrodiagnostische stimulator aan te sluiten</td></tr><tr><td>Helping Hands alligatorklem met ijzeren voet</td><td>Radio Shack</td><td>64-079</td><td>Onderhoud van de plaatsing van de registratie-elektrode</td></tr><tr><td>Isofluraan (250 mL fles)</td><td>Piramal Healthcare</td><td></td><td></td></tr><tr><td>Monoject gebogen tip irrigatie-slangpomp</td><td>Covidien</td><td>81412012</td><td>Gebruikt voor het aanbrengen van elektrodengel</td></tr><tr><td>PhysioSuite fysiologisch bewakingssysteem met RightTemp homeothermische opwarming</td><td>Kent Scientific Corporation</td><td>PS-RT</td><td>Inclusief infrarood opwarmingspad, rectale sonde en padtemperatuursonde</td></tr><tr><td>Pro trimmer Pet Grooming Kit</td><td>Oster</td><td>078577-010-003</td><td>Schaar voor haarverwijdering</td></tr><tr><td>Saporin (SAP)</td><td>Advanced Targeting Systems</td><td>PR-01</td><td>Gebruikt voor intrapleurale injectie (controlemiddel wanneer het alleen wordt geïnjecteerd)</td></tr><tr><td>Sierra Summit EMG-systeem</td><td>Cadwell Industries, Inc., Kennewick, WA</td><td></td><td>draagbaar elektrodiagnostisch systeem</td></tr><tr><td>SomnoSuite Low-Flow digitaal anesthesiesysteem</td><td>Kent Scientific Corporation</td><td>SOMNO</td><td>Inclusief anti-lek, anti-damplanteradapter; Y-adapterslang; koolstoffilter</td></tr><tr><td>Sprague-Dawley rat</td><td>Envigo kolonie 208a, Indianapolis, IN</td><td></td><td></td></tr><tr><td>Dierenarts ooftsalm op petroleumbasis</td><td>Puralube</td><td>26870</td><td>Toegepast tijdens anesthesie om hoornvliesletsel te voorkomen</td></tr></tbody></table>

beoordeling van de connectiviteitsuitkomstmaten van de motoreenheid van het rattendiafragma als kwantitatieve biomarkers van degeneratie en compensatie van phrenische motorneuronen

Assessing Rat Diaphragm Motor Unit Connectivity Outcome Measures as Quantitative Biomarkers of Phrenic Motor Neuron Degeneration and Compensation

Beoordeling van de connectiviteitsuitkomstmaten van de motoreenheid van het rattendiafragma als kwantitatieve biomarkers van degeneratie en compensatie van phrenische motorneuronen

Loss of ventilatory muscle function is a consequence of motor neuron injury and neurodegeneration (e.g., cervical spinal cord injury and amyotrophic ...

assessing-rat-diaphragm-motor-unit-connectivity-outcome-measures-as

Research

JoVE Journal

Neuroscience

Neurowetenschap

This video describes recording compound muscle action potentials (CMAPs) in diaphragm muscle fibers in a rat through phrenic nerve stimulation. A high-intensity electric pulse stimulation of the phrenic nerve generates action potentials in the diaphragm muscle fibers, which are observed by obtaining the response curve.

Measuring Compound Muscle Action Potentials by Stimulating the Phrenic Nerve in a Rat

De opname van samengestelde spieractiepotentialen beoordeelt kwantitatief de functionele diafragma-innervatie door frenicus motorneuronen. Immunohistochemie van het hele diafragma beoordeelt de morfologische innervatie op individuele neuromusculaire kruispunten. Het doel van dit protocol is om aan te tonen hoe deze twee krachtige methodologieën kunnen worden gebruikt in verschillende knaagdiermodellen van ruggenmergziekte.

Functionele en morfologische beoordeling van Diafragma Innervatie door diafragma Motor Neuronen

Respiratoire complicaties zijn de belangrijkste doodsoorzaak bij personen met cervicale dwarslaesie (cSCI). Diermodellen van cSCI zijn essentieel voor mechanistische evaluaties en preklinische studies. Hier introduceren we een reproduceerbare methode om functioneel herstel van de activiteit van de middenrifspier (DIAm) te beoordelen na unilaterale C2 spinale hemisectie (C2SH) bij ratten.

Beoordeling van functioneel herstel van eupneuïsche middenrifactiviteit na unilaterale cervicale ruggenmerghemisectie bij ratten

De laterale ventrikel muren bevatten de grootste germinal regio in de volwassen hersenen van zoogdieren. Traditioneel, hebben studies over neurogenese in deze regio zich op de klassieke opdeling technieken voor histologische analyse. Hier presenteren wij een alternatieve aanpak, de wholemount techniek, die een uitgebreide, en-face het oog op deze germinal regio biedt.

De subventriculaire zone En-face: Wholemount Beitsen en ependymale Flow

Larvale zebravis vormen de eerste gewervelde modelsysteem om gelijktijdig patch clamp opnamen toestaan ​​van een spinale motor-neuron en target skeletspieren. Deze video toont de microscopische methoden die worden gebruikt om een ​​segmentale CaP motor-neuron en doel spiercellen, alsook de methodologieën voor het opnemen van elk celtype te identificeren.

Gepaarde Patch Clamp Recordings van Motor-neuron en Target Skeletal Muscle in de zebravis

Gekweekte spiercellen een inadequate model om geïnnerveerde spier...

Een Functionele Motor-eenheid in de cultuur Dish: Co-cultuur van het ruggenmerg Explantaten-en spiercellen

De neuromusculaire junctie (NMJ) wordt gewijzigd in een verscheidenheid aan omstandigheden die soms kunnen resulteren in synaptische falen. Dit rapport beschrijft op fluorescentiemicroscoop gebaseerde methoden om dergelijke structurele veranderingen te kwantificeren.

De neuromusculaire junctie: Meten Synapse Maat, Fragmentatie en Veranderingen in Synaptic Protein dichtheid met behulp van confocale fluorescentie microscopie

Morfologie, grootte en locatie van intracellulaire organellen zijn evolutionair geconserveerd en lijken hun functie direct te beïnvloeden. Het begrijpen van de moleculaire mechanismen die ten grondslag liggen aan deze processen is een belangrijk doel geworden van de moderne biologie. Hier laten we zien hoe deze studies kunnen worden vergemakkelijkt door de toepassing van kwantitatieve technieken.

Waarom kwantificering Matters: karakterisering van fenotypes bij de<em&gt; Drosophila</em&gt; Larvale Neuromusculaire Junction

Hier beschrijven we een eenvoudige en breed toegankelijke methode om segmentale zenuwen in Drosophila larven te beschadigen om neurodegeneratie van motorische neuronen bij de neuromusculaire verbinding (NMJ) van derde instar larven te visualiseren en te kwantificeren.

Een eenvoudige neurologische mechanische letselmethode om te bestuderen<em&gt; Drosophila</em&gt; Motor Neuron Degeneration

Hier beschrijven we een protocol voor het identificeren van phrenic motorische neuronen in muizen nadat pleurale levering van fluorophore geconjugeerd cholera-toxine subeenheid bèta. Twee technieken worden vergeleken om te injecteren de pleurale Holte: transdiaphragmatic versus transthoracic benaderingen.

Retrograde neuroanatomische tracering van Phrenic motorische neuronen in muizen

Leer de MuNI-techniek voor het kwantificeren van motorische eenheden in het rattenmiddenrif. Deze niet-invasieve methode maakt longitudinale beoordeling van neuromusculaire functie mogelijk.

Beoordeling van de connectiviteitsuitkomstmaten van de motoreenheid van het rattendiafragma als kwantitatieve biomarkers van degeneratie en compensatie van phrenische motorneuronen (Video) | JoVE Journal

Beoordeling van de connectiviteitsuitkomstmaten van de motoreenheid van het rattendiafragma als kwantitatieve biomarkers van degeneratie en compensatie van phrenische motorneuronen

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