Name: Meting van het koolstofgehalte in luchtwegmacrofagen die zijn blootgesteld aan koolstofhoudende deeltjes (Video) | JoVE Journal
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Description: Leer een niet-invasieve methode om koolstofgehalte in luchtwegmacrofagen te meten als biomarker voor blootstelling aan koolstofdeeltjes. Dit protocol verbetert nauwkeurigheid en betrouwbaarheid bij individuele blootstellingsbeoordelingen.

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5 minutes

<meta charset="utf8"></head><body>Kwantificering van koolstof in pulmonale macrofagen via geïnduceerde sputumanalyse

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<table><tbody><tr><td>3 mL steriele rietjes</td><td>Shanghai YEASEN Biotechnology Co., LTD</td><td>84202ES03</td><td></td></tr><tr><td>50 mL centrifugebuisje</td><td>Thermo Fisher Scientific, USA</td><td>339652</td><td></td></tr><tr><td>Absolute ethyl alcohol</td><td>Sinopharm Group Chemical Reagent Co. LTD</td><td>64-17-5</td><td></td></tr><tr><td>Ceder olie</td><td>Shanghai McLean Biochemical Technology Co., LTD</td><td>C805296</td><td></td></tr><tr><td>Diff-quick kleuringsoplossing</td><td>Shanghai YEASEN Biotechnology Co., LTD</td><td>40748ES76</td><td></td></tr><tr><td>Dithiothreitol</td><td>Solebo Bio Co., LTD</td><td>D8220</td><td></td></tr><tr><td>Duchenne fosfaat buffer (DPBS)</td><td>Thermo Fisher Scientific, USA</td><td>14190144</td><td></td></tr><tr><td>Microscoop camera</td><td>Olympus Corporation of Japan</td><td>DP72</td><td></td></tr><tr><td>Neutraal boomgom</td><td>Solebo Bio Co., LTD</td><td>G8590</td><td></td></tr><tr><td>Nylon filtermembraan 70um</td><td>BD Falcon Bioscience, USA</td><td>211755</td><td></td></tr><tr><td>Optische microscoop</td><td>Olympus Corporation of Japan</td><td>BX60</td><td></td></tr><tr><td>Polyethyleenglycol</td><td>Sinopharm Group Chemical Reagent Co. LTD</td><td>25322-68-3</td><td></td></tr><tr><td>Ultracentrifuge</td><td>Thermo Fisher Scientific, USA</td><td>SL40R</td><td></td></tr><tr><td>Viscose diapositiva</td><td>Jiangsu SHitAI EXPERIMENTAL Equipment Co. LTD</td><td>188105</td><td></td></tr></tbody></table>

meting van het koolstofgehalte in luchtwegmacrofagen die zijn blootgesteld aan koolstofhoudende deeltjes

Measuring Carbon Content in Airway Macrophages Exposed to Carbon-Containing Particulate Matters

Meting van het koolstofgehalte in luchtwegmacrofagen die zijn blootgesteld aan koolstofhoudende deeltjes

Pulmonary macrophages exhibit a dose-dependent pattern in phagocytizing particles. Following engulfment, these macrophages are subsequently excreted with ...

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Meting van het koolstofgehalte in luchtwegmacrofagen die zijn blootgesteld aan koolstofhoudende deeltjes

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