Name: Systeem voor focaal, gesloten systeem letsel aan het centrale zenuwstelsel (Video) | JoVE Journal
Uploaded: 2024-11-29T13:20:00.000+00:00
Duration: 4 min 2 s
Description: Ontdek een kosteneffectief systeem voor gecontroleerde CNS-letsels bij muizen met een overpressie-luchtapparaat. Deze methode maakt precieze bestudering van axonale degeneratie en microgliale reacties mogelijk voor traumamechanismen.

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5 minutes

<meta charset="utf8"></head><body>Aangepast overdrukluchtsysteem voor het induceren van CZS-verwondingen in muizenmodellen

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<li>Gavett, B. E., Stern, R. A., Cantu, R. C., Nowinski, C. J., Mckee, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mild+traumatic+brain+injury%3A+A+risk+factor+for+neurodegeneration%5BTitle%5D)+AND+%22Alzheimers+Res+Ther%22%5BJournal%5D)">Mild traumatic brain injury: A risk factor for neurodegeneration.</a> Alzheimers Res Ther. 2, 1-3 (2010).</li>
<li>Smith, D. H., Johnson, V. E., Stewart, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+neuropathologies+of+single+and+repetitive+tbi%3A+Substrates+of+dementia%5BTitle%5D)+AND+%22Nat+Rev+Neurol%22%5BJournal%5D)">Chronic neuropathologies of single and repetitive tbi: Substrates of dementia.</a> Nat Rev Neurol. 9 (4), 211-221 (2013).</li>
<li>Juan, S. M., Daglas, M., Adlard, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tau+pathology%2C+metal+dyshomeostasis+and+repetitive+mild+traumatic+brain+injury%3A+An+unexplored+link+paving+the+way+for+neurodegeneration%5BTitle%5D)+AND+%22J+Neurotrauma%22%5BJournal%5D)">Tau pathology, metal dyshomeostasis and repetitive mild traumatic brain injury: An unexplored link paving the way for neurodegeneration.</a> J Neurotrauma. 39 (13-14), 902-922 (2022).</li>
<li>Kundi, S., Bicknell, R., Ahmed, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spinal+cord+injury%3A+Current+mammalian+models%5BTitle%5D)+AND+%22Am+J+Neurosci%22%5BJournal%5D)">Spinal cord injury: Current mammalian models.</a> Am J Neurosci. 4 (1), 1-12 (2013).</li>
<li>Flierl, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mouse+closed+head+injury+model+induced+by+a+weight-drop+device%5BTitle%5D)+AND+%22Nat+Protoc%22%5BJournal%5D)">Mouse closed head injury model induced by a weight-drop device.</a> Nat Protoc. 4 (9), 1328-1337 (2009).</li>
<li>Khalin, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+mouse+model+of+weight-drop+closed+head+injury%3A+Emphasis+on+cognitive+and+neurological+deficiency%5BTitle%5D)+AND+%22Neural+Regen+Res%22%5BJournal%5D)">A mouse model of weight-drop closed head injury: Emphasis on cognitive and neurological deficiency.</a> Neural Regen Res. 11 (4), 630-635 (2016).</li>
<li>Jamnia, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+clinically+relevant+closed-head+model+of+single+and+repeat+concussive+injury+in+the+adult+rat+using+a+controlled+cortical+impact+device%5BTitle%5D)+AND+%22J+Neurotrauma%22%5BJournal%5D)">A clinically relevant closed-head model of single and repeat concussive injury in the adult rat using a controlled cortical impact device.</a> J Neurotrauma. 34 (7), 1351-1363 (2017).</li>
<li>Mishra, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Primary+blast+causes+mild%2C+moderate%2C+severe+and+lethal+tbi+with+increasing+blast+overpressures%3A+Experimental+rat+injury+model%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Primary blast causes mild, moderate, severe and lethal tbi with increasing blast overpressures: Experimental rat injury model.</a> Sci Rep. 6 (1), 1-14 (2016).</li>
<li>Hu, C. -K., Chen, M. -H., Wang, Y. -H., Sun, J. -S., Wu, C. -Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Integration+of+multiple+prognostic+predictors+in+a+porcine+spinal+cord+injury+model%3A+A+further+step+closer+to+reality%5BTitle%5D)+AND+%22Front+Neurol%22%5BJournal%5D)">Integration of multiple prognostic predictors in a porcine spinal cord injury model: A further step closer to reality.</a> Front Neurol. 14, 1136267(2023).</li>
<li>Lim, J. -H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Establishment+of+a+canine+spinal+cord+injury+model+induced+by+epidural+balloon+compression%5BTitle%5D)+AND+%22J+Vet+Sci%22%5BJournal%5D)">Establishment of a canine spinal cord injury model induced by epidural balloon compression.</a> J Vet Sci. 8 (1), 89(2007).</li>
<li>Fukuda, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((New+canine+spinal+cord+injury+model+free+from+laminectomy%5BTitle%5D)+AND+%22Brain+Res+Protoc%22%5BJournal%5D)">New canine spinal cord injury model free from laminectomy.</a> Brain Res Protoc. 14 (3), 171-180 (2005).</li>
<li>Norris, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+closed-body+preclinical+model+to+investigate+blast-induced+spinal+cord+injury%5BTitle%5D)+AND+%22Front+Mol+Neurosci%22%5BJournal%5D)">A closed-body preclinical model to investigate blast-induced spinal cord injury.</a> Front Mol Neurosci. 16, 1199732(2023).</li>
<li>Zhao, Q., Zhang, J., Li, H., Li, H., Xie, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Models+of+traumatic+brain+injury-highlights+and+drawbacks%5BTitle%5D)+AND+%22Front+Neurol%22%5BJournal%5D)">Models of traumatic brain injury-highlights and drawbacks.</a> Front Neurol. 14, 1151660(2023).</li>
<li>Osier, N. D., Dixon, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+controlled+cortical+impact+model%3A+Applications%2C+considerations+for+researchers%2C+and+future+directions%5BTitle%5D)+AND+%22Front+Neurol%22%5BJournal%5D)">The controlled cortical impact model: Applications, considerations for researchers, and future directions.</a> Front Neurol. 7, 214364(2016).</li>
<li>Chen, Y., Constantini, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Caveats+for+using+shock+tube+in+blast-induced+traumatic+brain+injury+research%5BTitle%5D)+AND+%22Front+Neurol%22%5BJournal%5D)">Caveats for using shock tube in blast-induced traumatic brain injury research.</a> Front Neurol. 4, 117(2013).</li>
<li>Bernardo-Colón, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Antioxidants+prevent+inflammation+and+preserve+the+optic+projection+and+visual+function+in+experimental+neurotrauma%5BTitle%5D)+AND+%22Cell+Death+Dis%22%5BJournal%5D)">Antioxidants prevent inflammation and preserve the optic projection and visual function in experimental neurotrauma.</a> Cell Death Dis. 9 (11), 1097(2018).</li>
<li>Bernardo-Colón, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Progression+and+pathology+of+traumatic+optic+neuropathy+from+repeated+primary+blast+exposure%5BTitle%5D)+AND+%22Front+Neurosci%22%5BJournal%5D)">Progression and pathology of traumatic optic neuropathy from repeated primary blast exposure.</a> Front Neurosci. 13, 719(2019).</li>
<li>Thomas, C. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+necroptosis+in+the+retina+in+a+repeated+primary+ocular+blast+injury+mouse+model%5BTitle%5D)+AND+%22Exp+Eye+Res%22%5BJournal%5D)">Assessment of necroptosis in the retina in a repeated primary ocular blast injury mouse model.</a> Exp Eye Res. 197, 108102(2020).</li>
<li>Naguib, S., Bernardo-Colón, A., Rex, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intravitreal+injection+worsens+outcomes+in+a+mouse+model+of+indirect+traumatic+optic+neuropathy+from+closed+globe+injury%5BTitle%5D)+AND+%22Exp+Eye+Res%22%5BJournal%5D)">Intravitreal injection worsens outcomes in a mouse model of indirect traumatic optic neuropathy from closed globe injury.</a> Exp Eye Res. 202, 108369(2021).</li>
<li>Vest, V., Bernardo-Colón, A., Watkins, D., Kim, B., Rex, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+repeat+exposure+to+subthreshold+trauma+causes+synergistic+axonal+damage+and+functional+deficits+in+the+visual+pathway+in+a+mouse+model%5BTitle%5D)+AND+%22J+Neurotrauma%22%5BJournal%5D)">Rapid repeat exposure to subthreshold trauma causes synergistic axonal damage and functional deficits in the visual pathway in a mouse model.</a> J Neurotrauma. 36 (10), 1646-1654 (2019).</li>
<li>Hines-Beard, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+mouse+model+of+ocular+blast+injury+that+induces+closed+globe+anterior+and+posterior+pole+damage%5BTitle%5D)+AND+%22Exp+Eye+Res%22%5BJournal%5D)">A mouse model of ocular blast injury that induces closed globe anterior and posterior pole damage.</a> Exp Eye Res. 99, 63-70 (2012).</li>
<li>Naguib, S., Bernardo-Colon, A., Cencer, C., Rex, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Galantamine+confers+neuroprotection+in+a+model+of+indirect+traumatic+optic+neuropathy%5BTitle%5D)+AND+%22Invest+Ophthalmol+Vis+Sci%22%5BJournal%5D)">Galantamine confers neuroprotection in a model of indirect traumatic optic neuropathy.</a> Invest Ophthalmol Vis Sci. 60 (9), 4407-4407 (2019).</li>
<li>Bricker-Anthony, C., Hines-Beard, J., Rex, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Molecular+changes+and+vision+loss+in+a+mouse+model+of+closed-globe+blast+trauma%5BTitle%5D)+AND+%22Invest+Ophthalmol+Vis+Sci%22%5BJournal%5D)">Molecular changes and vision loss in a mouse model of closed-globe blast trauma.</a> Invest Ophthalmol Vis Sci. 55 (8), 4853-4862 (2014).</li>
<li>Bricker-Anthony, C., Hines-Beard, J., D'surney, L., Rex, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exacerbation+of+blast-induced+ocular+trauma+by+an+immune+response%5BTitle%5D)+AND+%22J+Neuroinflammation%22%5BJournal%5D)">Exacerbation of blast-induced ocular trauma by an immune response.</a> J Neuroinflammation. 11 (1), 1-15 (2014).</li>
<li>Bricker-Anthony, C., Hines-Beard, J., Rex, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Eye-directed+overpressure+airwave-induced+trauma+causes+lasting+damage+to+the+anterior+and+posterior+globe%3A+A+model+for+testing+cell-based+therapies%5BTitle%5D)+AND+%22J+Ocul+Pharmacol+Ther%22%5BJournal%5D)">Eye-directed overpressure airwave-induced trauma causes lasting damage to the anterior and posterior globe: A model for testing cell-based therapies.</a> J Ocul Pharmacol Ther. 32 (5), 286-295 (2016).</li>
<li>Bricker-Anthony, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Erythropoietin+either+prevents+or+exacerbates+retinal+damage+from+eye+trauma+depending+on+treatment+timing%5BTitle%5D)+AND+%22Optom+Vis+Sci%22%5BJournal%5D)">Erythropoietin either prevents or exacerbates retinal damage from eye trauma depending on treatment timing.</a> Optom Vis Sci. 94 (1), 20-32 (2017).</li>
<li>Heldt, S. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+closed-head+model+of+mild+traumatic+brain+injury+caused+by+primary+overpressure+blast+to+the+cranium+produces+sustained+emotional+deficits+in+mice%5BTitle%5D)+AND+%22Front+Neurol%22%5BJournal%5D)">A novel closed-head model of mild traumatic brain injury caused by primary overpressure blast to the cranium produces sustained emotional deficits in mice.</a> Front Neurol. 5, 2(2014).</li>
<li>Guley, N. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+closed-head+model+of+mild+traumatic+brain+injury+using+focal+primary+overpressure+blast+to+the+cranium+in+mice%5BTitle%5D)+AND+%22J+Neurotrauma%22%5BJournal%5D)">A novel closed-head model of mild traumatic brain injury using focal primary overpressure blast to the cranium in mice.</a> J Neurotrauma. 33 (4), 403-422 (2016).</li>
<li>Del Mar, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+closed-body+model+of+spinal+cord+injury+caused+by+high-pressure+air+blasts+produces+extensive+axonal+injury+and+motor+impairments%5BTitle%5D)+AND+%22Exp+Neurol%22%5BJournal%5D)">A novel closed-body model of spinal cord injury caused by high-pressure air blasts produces extensive axonal injury and motor impairments.</a> Exp Neurol. 271, 53-71 (2015).</li>
<li>Wolf, S. J., Bebarta, V. S., Bonnett, C. J., Pons, P. T., Cantrill, S. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Blast+injuries%5BTitle%5D)+AND+%22Lancet%22%5BJournal%5D)">Blast injuries.</a> Lancet. 374 (9687), 405-415 (2009).</li>
<li>Bricker-Anthony, C., Rex, T. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neurodegeneration+and+vision+loss+after+mild+blunt+trauma+in+the+c57bl%2F6+and+dba%2F2j+mouse%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Neurodegeneration and vision loss after mild blunt trauma in the c57bl/6 and dba/2j mouse.</a> PLoS One. 10 (7), e0131921(2015).</li>
<li>Heldt, S. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+closed-head+model+of+mild+traumatic+brain+injury+caused+by+primary+overpressure+blast+to+the+cranium+produces+sustained+emotional+deficits+in+mice%5BTitle%5D)+AND+%22Front+Neurol%22%5BJournal%5D)">A novel closed-head model of mild traumatic brain injury caused by primary overpressure blast to the cranium produces sustained emotional deficits in mice.</a> Front Neurol. 5, 2(2014).</li>
</ol>

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<table>
<tbody>
<tr>
<td>1-Pentanol</td>
<td>Fisher Scientific</td>
<td>AC160600250</td>
<td>Gebruikt om Avertin-oplossing te maken </td>
</tr>
<tr>
<td>2,2,2-tribomethylethanol</td>
<td>Sigma Aldrich</td>
<td>T48402</td>
<td>Gebruikt om Avertin-oplossing te maken </td>
</tr>
<tr>
<td>24-well platen met deksel</td>
<td>VWR</td>
<td>76520-634</td>
<td>24-well plaat</td>
</tr>
<tr>
<td>2-Propanol </td>
<td>Fisher Scientific</td>
<td>A451-1 </td>
<td></td>
</tr>
<tr>
<td>50 kS/s/kanaal Bridge Analog Input Module </td>
<td>National Instruments</td>
<td>NI-9237</td>
<td>DAQ module</td>
</tr>
<tr>
<td>Albumine bovien Fractie V (BSA) </td>
<td>Research Products International </td>
<td>A30075</td>
<td>BSA</td>
</tr>
<tr>
<td>Anti-Iba1 primaire antistof (Geiten polyklonaal) </td>
<td>Abcam </td>
<td>ab5076</td>
<td>Marker voor microglia, Gebruikt op 1:500 concentratie </td>
</tr>
<tr>
<td>Anti-Synaptophysin primaire antistof (Muis monoklonaal) </td>
<td>Abcam </td>
<td>ab8049</td>
<td>Marker voor fotoreceptor ribbon synapsen, Gebruikt op 1:20 concentratie</td>
</tr>
<tr>
<td>Araldite GY 502 </td>
<td>Electron Microscopy Sciences</td>
<td>10900</td>
<td></td>
</tr>
<tr>
<td>Cacodylate buffer</td>
<td>Electron Microscopy Sciences</td>
<td>11652</td>
<td></td>
</tr>
<tr>
<td>Houtskoolfilterbus</td>
<td>E-Z Systems</td>
<td>EZ-258</td>
<td>Verzameling van anesthesieafval</td>
</tr>
<tr>
<td>Clear H20 DietGel 76A</td>
<td>Clear H2O </td>
<td>72-07-5022</td>
<td>Gebruikt na de ontploffing om dierlijk herstel te bevorderen</td>
</tr>
<tr>
<td>CompactDAQ Chassis</td>
<td>National Instruments</td>
<td>USB-9162</td>
<td>DAQ chassis</td>
</tr>
<tr>
<td>Perslucht</td>
<td>A-L Gas</td>
<td>GSMCA300 </td>
<td>Gebruikt om persluchttank bij te vullen</td>
</tr>
<tr>
<td>DAPI Fluoromount-G </td>
<td>Southern Biotech</td>
<td></td>
<td>Montage media met DAPI</td>
</tr>
<tr>
<td>Diamantzaagknijptang</td>
<td>Micro Star Technologies, Group of Bruker Nano, Inc. </td>
<td></td>
<td>Voor het doorsnijden van oogzenuwen, 3 mm/45 graden/Stijl H </td>
</tr>
<tr>
<td>Ezel Anti-Geiten IgG (H+L) Hoog Geadsorbeerd Secundair Antilichaam, Alex Fluor 594</td>
<td>Invitrogen (Leverancier: Fisher Scientific)</td>
<td>A-11058</td>
<td>Secundair antilichaam voor microglia, Gebruikt op 1:200 concentratie</td>
</tr>
<tr>
<td>Ezel Anti-Muis IgG (H+L) Hoog Geadsorbeerd Secundair Antilichaam, Alex Fluor 594</td>
<td>Invitrogen (Leverancier: Fisher Scientific)</td>
<td>A-21203</td>
<td>Secundair antilichaam voor fotoreceptor ribbon synapsen, Gebruikt op 1:200 concentratie</td>
</tr>
<tr>
<td>Ezel Anti-Konijn IgG (H+L) Hoog Geadsorbeerd Secundair Antilichaam, Alex Fluor 488</td>
<td>Invitrogen (Leverancier: Fisher Scientific)</td>
<td>A-21206</td>
<td>Secundair antilichaam voor staafjesbipolare cellen, Gebruikt op 1:200 concentratie </td>
</tr>
<tr>
<td>Ezelserum </td>
<td>Sigma Aldrich </td>
<td>D9662</td>
<td>NDS</td>
</tr>
<tr>
<td>Dumont #3 Tang</td>
<td>Fine Science Tools</td>
<td>11231-30</td>
<td>Fijne pincet voor gehele ogenukleatie </td>
</tr>
<tr>
<td>Ethanol (200 proof) </td>
<td>KOPTEC (Leverancier: VWR) </td>
<td>89125-188</td>
<td>Ethanol </td>
</tr>
<tr>
<td>Fluoromount-G </td>
<td>Invitrogen (Leverancier: Fisher Scientific)</td>
<td>00-4958-02</td>
<td>Montage media</td>
</tr>
<tr>
<td>Genteal Tears Ophthalmisch Gel</td>
<td>Covetrus</td>
<td>72359</td>
<td>Oogsmering om de ogen te voorkomen dat ze uitdrogen tijdens/na anesthesie </td>
</tr>
<tr>
<td>Glutaraldehyde</td>
<td>Electron Microscopy Sciences</td>
<td>16200</td>
<td></td>
</tr>
<tr>
<td>Meetcilinder 1000 mL</td>
<td>Fisher Scientific</td>
<td>08-572G</td>
<td></td>
</tr>
<tr>
<td>Meetcilinder 250 mL</td>
<td>Fisher Scientific</td>
<td>08-572E</td>
<td></td>
</tr>
<tr>
<td>Meetcilinder 500 mL</td>
<td>Fisher Scientific</td>
<td>08-572F</td>
<td></td>
</tr>
<tr>
<td>Verwarmingskussen </td>
<td>Braintree Scientific</td>
<td>AP-R 26E</td>
<td>Gecontroleerde verwarmingsondersteuning</td>
</tr>
<tr>
<td>Hogedrukvulstation</td>
<td>Ninja Paintball</td>
<td>HPFSV2</td>
<td>Gebruikt om persluchttank bij te vullen</td>
</tr>
<tr>
<td>ImageJ</td>
<td>National Institutes of Health </td>
<td></td>
<td>Beeldanalyse software</td>
</tr>
<tr>
<td>Invert Mini</td>
<td>Empire Paintball</td>
<td></td>
<td>Paintballpistool</td>
</tr>
<tr>
<td>Isofluraan</td>
<td>Covetrus</td>
<td>29405</td>
<td>Inhalatieanesthesie</td>
</tr>
<tr>
<td>Isofluraanverdamper</td>
<td>VetEquip</td>
<td>901806</td>
<td>Dierenanesthesie</td>
</tr>
<tr>
<td>Masterflex Pomp</td>
<td>Cole-Parmer</td>
<td></td>
<td>Gebruikt voor dierlijke perfusie</td>
</tr>
<tr>
<td>Methanol </td>
<td>Sigma Aldrich</td>
<td>322415-2L </td>
<td></td>
</tr>
<tr>
<td>Microscoopglazen</td>
<td>Globe Scientific</td>
<td>1358W</td>
<td>Witte glazen microscoopglazen</td>
</tr>
<tr>
<td>NI Measurement and Automation Explorer (NI MAX) </td>
<td>National Instruments</td>
<td></td>
<td>Software om DAQ-systeem instellingen te configureren</td>
</tr>
<tr>
<td>NI-DAQmx drivers </td>
<td>National Instruments</td>
<td></td>
<td>Driver voor interactie met DAQ-systeem </td>
</tr>
<tr>
<td>Nikon Eclipse Ni-E microscoop</td>
<td>Nikon Instruments</td>
<td></td>
<td></td>
</tr>
<tr>
<td>Osmiumtetraoxide 2%</td>
<td>Electron Microscopy Sciences</td>
<td>19152</td>
<td></td>
</tr>
<tr>
<td>Paraformaldehyde 32%</td>
<td>Electron Microscopy Sciences</td>
<td>15714-S</td>
<td>PFA verdund tot 4%</td>
</tr>
<tr>
<td>Parafenyleendiamine </td>
<td>Sigma Aldrich</td>
<td>P6001</td>
<td></td>
</tr>
<tr>
<td>PBS (10x), pH 7.4</td>
<td>Thermo Fisher Scientific</td>
<td>70011044</td>
<td>PBS verdund tot 1x</td>
</tr>
<tr>
<td>Propyleenoxide </td>
<td>Electro

systeem voor focaal, gesloten systeem letsel aan het centrale zenuwstelsel

System for Focal, Closed-System Central Nervous System Injury

Systeem voor focaal, gesloten systeem letsel aan het centrale zenuwstelsel

The prevalence of closed-system central nervous system (CNS) injuries underscores the need for an enhanced understanding of these traumas to improve ...

system-for-focal-closed-system-central-nervous-system-injury

Research

JoVE Journal

Neuroscience

Neurowetenschap

Laterale vloeistof percussie (LFP), een uitgewerkt model van traumatisch hersenletsel bij muizen, is aangetoond. LFP vervult drie belangrijke criteria voor dierlijke modellen: validiteit, betrouwbaarheid en klinische relevantie. De procedure, bestaande uit chirurgische craniotomie, fixatie van de hub, gevolgd door inductie van letsels die zouden ontstaan ​​bij focale en diffuse blessures, is beschreven.

Laterale Fluid Percussie: Model van traumatisch hersenletsel bij muizen

Source: Shahror, R. A. et. al., Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model. J. Vis. Exp. (2019)This video demonstrates intranasal mesenchymal stem cell (MSC) delivery in a mouse model. MSCs absorb into nasal tissue, migrate to the brain via cytokine signals, and secrete neurotrophic factors at the injury site, offering neurotherapeutic potential for neuronal repair.

Intranasal Mesenchymal Stem Cell Delivery in a Mouse Model of Traumatic Brain Injury

Vertebrale stabilisatie is noodzakelijk voor het minimaliseren van variabiliteit en voor het produceren van consistente experimentele ruggenmergletsels. Met een aangepaste stabiliserende inrichting in combinatie met de NYU / Mascis slaginrichting, hebben we hier aangetoond dat de juiste apparatuur en procedure voor het genereren reproduceerbare hemi-contusive cervicale (C5) ruggenmergletsel bij volwassen ratten.

A Novel Vertebral Stabilization werkwijze voor het produceren Contusive Spinal Cord Injury

Fibrine matrices bevattende groeifactoren zijn gebruikt om geënte neurale stamcellen behouden in locaties volledig ruggenmerg doorsnijding. Geënte cellen volledig gevuld de laesie holte en gedifferentieerd in meerdere neurale celtypes, waaronder neuronen die axons verlengd gastheer ruggenmerg over lange afstanden.

Bevordering van Survival en differentiatie van neurale stamcellen met fibrine en groeifactor Cocktails na Ernstige Spinal Cord Injury

Traumatische hersenverwondingen (THV) blijven een ernstig gezondheidsprobleem. Met behulp van het gecontroleerde corticale impactchirurgie-model kan onderzoek worden gedaan naar de effecten van THV en mogelijke behandelmethoden.

Gecontroleerde Corticale Impact Model voor traumatisch hersenletsel

De behandeling van cervicale spinale-koordletsel met zowel zelfassemblerende peptiden (SAP) en neurale voorlopercellen (NPC), samen met groeifactoren, is een veelbelovende aanpak om regeneratie en herstel te bevorderen. Een contusie/compressie aneurysma clip ratmodel van cervicale SCI en gecombineerde behandeling waarbij SAP injectie en NPC transplantatie is vastgesteld.

Synergetische gebruik van neurale precursoren en Zelfgeassembleerde Peptiden in Experimentele Baarmoederhalskanker Spinal Cord Injury

Hier tonen we de prestaties van een minimale ruggenmerg letsel model in een volwassen muis die de centrale kanaal niche huisvesting endogene neurale stamcellen (NSCs spaart). Laten we zien hoe de bepaling van de neurosphere kan worden gebruikt voor het kwantificeren van activering en migratie van definitieve en primitieve NSCs na verwonding.

Een test van de Neurosphere voor de evaluatie van de activering van de endogene neurale stamcellen in een muismodel van minimale dwarslaesie

Een methode voor het opnemen van multimodaliteit monitoring signalen bij patiënten met ernstige hersenletsel met behulp van een nachtkastje, één burr gat techniek is beschreven.

Een bed, een Burr gat benadering van multimodaliteit toezicht op ernstige hersenletsel

Hier presenteren we een protocol om ernstige TBI te induceren met de laterale vloeistof percussie blessure (FPI) model bij volwassen, mannelijke Wistar ratten. We tonen ook het gebruik van een draadloze telemetrie systeem om continu video-EEG-opnames en monitor voor epileptiform lozingen in overeenstemming met post-traumatische epileptogenese te verzamelen.

Gebruik van een draadloze video-EEG-systeem te controleren epileptiform lozingen na laterale vloeistof-percussie geïnduceerde traumatisch hersenletsel

Hier beschrijven we een protocol voor de inductie van muriene traumatisch hersenletsel via een open-Head gecontroleerd corticale impact.

Murine model van gecontroleerde corticale impact voor de inductie van traumatisch hersenletsel

Dit protocol presenteert methoden om de neuro-inflammatoire en hemodynamische respons op mild traumatisch hersenletsel te karakteriseren en om deze gegevens te integreren als onderdeel van een multivariate systeemanalyse met behulp van partiële kleinste kwadratenregressie.

Systeemanalyse van de neuro-inflammatoire en hemodynamische respons op traumatisch hersenletsel

Ontdek een kosteneffectief systeem voor gecontroleerde CNS-letsels bij muizen met een overpressie-luchtapparaat. Deze methode maakt precieze bestudering van axonale degeneratie en microgliale reacties mogelijk voor traumamechanismen.

Systeem voor focaal, gesloten systeem letsel aan het centrale zenuwstelsel

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