Name: In vivo visualisatie van calciumtransiënten tijdens bevruchting en vroege ontwikkeling in C. elegans (Video) | JoVE Journal
Uploaded: 2024-07-12T15:00:00.000+00:00
Duration: 5 min 11 s
Description: Ontdek de CaFE-reporter voor visualisatie van calciumdynamiek tijdens bevruchting en vroege embryogenese in C. elegans. Deze methode onthult vertraagde calciumgolven en enkelceltransiënten.

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<meta charset="utf8"></head><body>Calcium volgen in de vroege ontwikkelingsfasen van C. elegans

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<table><tbody><tr><td>Agar</td><td>Fisher Scientific</td><td>DF0140-07-4</td><td>2 kg; Poeder lost gemakkelijker op dan vlokken</td></tr><tr><td>Agarose</td><td>MidSci</td><td>BE-A125</td><td>500 g</td></tr><tr><td>Alcohol lamp</td><td>Fisher Scientific</td><td>S13475</td><td>Gebruik met 95% ethanol</td></tr><tr><td>Ammonium chloride (NH4Cl)</td><td>Fisher Scientific</td><td>AAA1500030</td><td>250 g</td></tr><tr><td>Ampicillin</td><td>Fisher Scientific</td><td>BP1760-5</td><td>5 g</td></tr><tr><td>AMSCO 400 Series Small Steam Sterilizer</td><td>Steris Healthcare</td><td>N/A</td><td></td></tr><tr><td>Bacto-peptone</td><td>Fisher Scientific</td><td>BP1420-500</td><td>500 g</td></tr><tr><td>Bacto-tryptone</td><td>Fisher Scientific</td><td>DF0123-17-3</td><td>500 g</td></tr><tr><td>Calcium chloride (CaCl2)</td><td>Fisher Scientific</td><td>C69-500</td><td>500 g</td></tr><tr><td>Cholesterol</td><td>Thermo Scientific</td><td>A11470.18</td><td>50 g</td></tr><tr><td>Dragonfly 200 spinning disk confocal</td><td>Oxford Instruments Andor</td><td>N/A</td><td>Gebruikt met Leica microscoop</td></tr><tr><td>Fisherbrand Superfrost Cytogenics Microscope Slides</td><td>Fisher Scientific</td><td>22-035-900</td><td>144 dia's per pak</td></tr><tr><td>Flat Nose Pliers, Smooth Jaw</td><td>Home Depot</td><td>305530604</td><td>Zorg ervoor dat de pincetjes een gladde kaak hebben</td></tr><tr><td>Isopropyl &beta;- d-1-thiogalactopyranoside&nbsp;(IPTG)</td><td>Fisher Scientific</td><td>BP1755-10</td><td>10 g; vrij van dioxaan</td></tr><tr><td>Laboratory tape</td><td>Fisher Scientific</td><td>15-901-10R</td><td>0,5 inch tape wordt gebruikt om microscoopglazen te lijmen</td></tr><tr><td>Levamisole</td><td>Fisher Scientific</td><td>AC187870100</td><td>10 g</td></tr><tr><td>Magnesium sulfate (MgSO4)</td><td>Fisher Scientific</td><td>M63-500</td><td>500 g</td></tr><tr><td>Microscope cover glass</td><td>Fisher Scientific</td><td>12541016</td><td>1 oz pak</td></tr><tr><td>Nikon ECLIPSE Ti2 laser scanning confocal</td><td>Nikon</td><td>N/A</td><td></td></tr><tr><td>Nikon NIS Elements software</td><td>Nikon</td><td>N/A</td><td>Confocal</td></tr><tr><td>OP50 Escherichia coli</td><td>Caenorhabditis Genetics Center (CGC)</td><td>OP50</td><td></td></tr><tr><td>Platinum Wire</td><td>TriTech</td><td>PT-9010</td><td></td></tr><tr><td>Potassium phosphate dibasic (K2HPO4)</td><td>Fisher Scientific</td><td>P288-500</td><td>500 g</td></tr><tr><td>Potassium phosphate monobasic (KH2PO4)</td><td>Fisher Scientific</td><td>AA1159436</td><td>500 g</td></tr><tr><td>Sodium chloride (NaCl)</td><td>Fisher Scientific</td><td>S271-500</td><td>500 g</td></tr><tr><td>Sodium phosphate dibasic heptahydrate (Na2HPO4)</td><td>Fisher Scientific</td><td>S471-3</td><td>3 kg</td></tr><tr><td>Stereo microscope</td><td>Leica</td><td>KL300 LED</td><td></td></tr><tr><td>Sterile Petri dish (35 mm x 10 mm)</td><td>CellTreat</td><td>229638</td><td>960 petrischalen per doos</td></tr><tr><td>Sterile Petri dish (60 mm x 15 mm)</td><td>CellTreat</td><td>229665</td><td>500 petrischalen per doos</td></tr><tr><td>Strain EAG16 spn-4p::jGCaMP7s::pie-1u</td><td>Caenorhabditis Genetics Center (CGC)</td><td>EAG16</td><td>Gemaakt door Kim Guisbert Lab</td></tr><tr><td>Strain EAG25 spn-4p::jGCaMP7s::pie-1u; ujIs113 II.</td><td>Caenorhabditis Genetics Center (CGC)</td><td>EAG25</td><td>Gemaakt door Kim Guisbert Lab</td></tr><tr><td>Strain EAG28 spn-4p::jGCaMP7s::pie-1u; unc-119(ed3) III; ltIs44 V.</td><td>Caenorhabditis Genetics Center (CGC)</td><td>EAG28</td><td>Gemaakt door Kim Guisbert Lab</td></tr><tr><td>Strain JIM113 ujIs113 II [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)]</td><td>Caenorhabditis Genetics Center (CGC)</td><td>JIM113</td><td>Gemaakt door E. Preston - Murray Lab</td></tr><tr><td>Strain OD70 unc-119(ed3) III; ltIs44 V [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)]</td><td>Caenorhabditis Genetics Center (CGC)</td><td>OD70</td><td>Gemaakt door Audhya/Oegema - Greenstein Lab</td></tr><tr><td>Tritech Worm Pick Handle</td><td>TriTech</td><td>TWPH1</td><td></td></tr><tr><td>Yeast extract</td><td>IBI Scientific</td><td>IB49160</td><td>500 g</td></tr></tbody></table>

in vivo visualisatie van calciumtransiënten tijdens bevruchting en vroege ontwikkeling in c. elegans

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In vivo visualisatie van calciumtransiënten tijdens bevruchting en vroege ontwikkeling in C. elegans

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Ontdek de CaFE-reporter voor visualisatie van calciumdynamiek tijdens bevruchting en vroege embryogenese in C. elegans. Deze methode onthult vertraagde calciumgolven en enkelceltransiënten.

In vivo visualisatie van calciumtransiënten tijdens bevruchting en vroege ontwikkeling in C. elegans

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