Name: Ontwikkeling en toepassing van rapamycine-gereguleerde tyrosinefosfatasen (Video) | JoVE Journal
Uploaded: 2024-09-06T14:10:00.000+00:00
Duration: 6 min 56 s
Description: Ontdek hoe rapamycine-gereguleerde tyrosinefosfatasen de activiteit van SHIP-2 in HEK-293T-cellen tijdelijk en specifiek reguleren. Deze methode onthult mechanismen van celspreiding en migratie.

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<meta charset="utf8"></head><body>Temporele regulatie van fosfatase-activiteit met het rapamycine-gereguleerde systeem

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<li>Mercan, F., Bennett, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analysis+of+protein+tyrosine+phosphatases+and+substrates%5BTitle%5D)+AND+%22Curr+Protoc+Mol+Biol%22%5BJournal%5D)">Analysis of protein tyrosine phosphatases and substrates.</a> Curr Protoc Mol Biol. 18, Unit 18.16 (2010).</li>
<li>Karginov, A. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Light+regulation+of+protein+dimerization+and+kinase+activity+in+living+cells+using+photocaged+rapamycin+and+engineered+FKBP%5BTitle%5D)+AND+%22J+Am+Chem+Soc%22%5BJournal%5D)">Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP.</a> J Am Chem Soc. 133 (3), 420-423 (2011).</li>
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ontwikkeling en toepassing van rapamycine-gereguleerde tyrosinefosfatasen

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Ontwikkeling en toepassing van rapamycine-gereguleerde tyrosinefosfatasen

Tyrosine phosphatases are an important family of enzymes that regulate critical physiological functions. They are often dysregulated in human diseases, ...

development-application-rapamycin-regulated-tyrosine

Research

JoVE Journal

Biology

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Jeffrey D. Rothstein spreekt over de pathologie en de mechanismen die ten grondslag liggen aan amyotrofische laterale sclerose of ALS, ALS vooruitgang in onderzoek en de huidige strategieën voor de ontwikkeling van therapieën.

ALS - Motor Neuron Disease: Mechanisme en ontwikkeling van nieuwe therapieën

Dit artikel beschrijft een nieuw protocol en reagensset ontworpen voor de gevoelige meting van de neurotoxische effecten van verbindingen en behandelingen op co-culturen van neuronen en astrocyten met behulp van hoge inhoudsanalyse. Resultaten tonen aan dat de hoge inhoudelijke analyse een spannende nieuwe technologie voor neurotoxiciteit assessment vertegenwoordigt.

Een Neuronale en astrocyte co-cultuur assay voor High Content Analysis van neurotoxiciteit

Mammatumor cellen die luciferase worden subcutaan geïmplanteerd in muizen en gevisualiseerd met behulp van optische beeldvorming om tumoren groei en ontwikkeling niet-invasief in een longitudinale studie monitor.

In vivo Bioluminescent Imaging van borsttumoren gebruik van IVIS Spectrum

Dit protocol laat zien hoe het behoud van gezonde, ongedifferentieerde menselijke embryonale stamcellen (ES) cellen.

Cultuur en onderhoud van menselijke embryonale stamcellen

Ontdek hoe rapamycine-gereguleerde tyrosinefosfatasen de activiteit van SHIP-2 in HEK-293T-cellen tijdelijk en specifiek reguleren. Deze methode onthult mechanismen van celspreiding en migratie.

Ontwikkeling en toepassing van rapamycine-gereguleerde tyrosinefosfatasen

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