Name: Karakterisering van gemedieerde extracellulaire elektronenoverdracht in melkzuurbacteriën met een bio-elektrochemisch systeem met drie elektroden en twee kamers (Video) | JoVE Journal
Uploaded: 2024-08-23T14:50:00.000+00:00
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Description: Ontdek hoe gemedieerde extracellulaire elektronenoverdracht in Lactiplantibacillus plantarum wordt gekarakteriseerd met een bio-elektrochemisch systeem met drie elektroden en twee kamers.

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<meta charset="utf8"></head><body>Chronoamperometrie Analyse van EET in Lactiplantibacillus plantarum met DHNA

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</ol>

<table><tbody><tr><td>1,4-Dihydroxy-2-naphthoic acid (DHNA)</td><td>Sigma-Aldrich</td><td>281255-25G</td><td></td></tr><tr><td>1.0 mm diameter titanium wire</td><td>Thermo Fisher Scientific</td><td>045485.BY</td><td>Op maat gesneden voor werk- en tegenrelektoren</td></tr><tr><td>120-C Aluminum Oxide Sheets 9" x 11"</td><td>Johnson Abrasives</td><td>10108-15</td><td></td></tr><tr><td>3 mL plastic syringes</td><td>Thermo Fisher Scientific</td><td>14955457</td><td></td></tr><tr><td>3M KCl solution saturated with silver chloride</td><td>Millipore Sigma</td><td>60137-250ML</td><td></td></tr><tr><td>6.35-mm-thick carbon felt</td><td>Thermo Fisher Scientific</td><td>043200.RF</td><td>Snijden in 16 cm2 rondjes</td></tr><tr><td>Ag/AgCl reference electrode</td><td>CH Instruments</td><td>CH111</td><td></td></tr><tr><td>Air-Tite Premium Hypodermic Needles</td><td>Thermo Fisher Scientific</td><td>14-817-102</td><td></td></tr><tr><td>AlK(SO)4 * 12H2O</td><td>Sigma-Aldrich</td><td>237086-100G</td><td></td></tr><tr><td>Ammonium citrate tribasic</td><td>Millipore Sigma</td><td>A1332</td><td></td></tr><tr><td>Avanti J-15R Centrifuge</td><td>Beckman Coulter</td><td>B99517</td><td></td></tr><tr><td>BD Precision Glide Needle, 18 G x 1 inch</td><td>Thermo Fisher Scientific</td><td>14-826-5G</td><td></td></tr><tr><td>BD Precision Glide Needle, 21 G x 2 inch</td><td>Thermo Fisher Scientific</td><td>14-821-13N</td><td></td></tr><tr><td>Bel-Art SP Scienceware Cleanware Aqua-Clear Water Condtioner</td><td>Thermo Fisher Scientific</td><td>23-278339</td><td></td></tr><tr><td>Biotin</td><td>Millipore Sigma</td><td>B4639</td><td></td></tr><tr><td>CaCl2</td><td>Millipore Sigma</td><td>C4901</td><td></td></tr><tr><td>Calcium D-(+)-pantothenate</td><td>Millipore Sigma</td><td>1087009</td><td></td></tr><tr><td>Casamino acids</td><td>Millipore Sigma</td><td>2240-OP</td><td></td></tr><tr><td>cation exchange membrane</td><td>Membranes International</td><td>CMI-7000</td><td>Snijden in rondjes passend bij de BES O-ring</td></tr><tr><td>CoCl2 * 6H2O</td><td>Millipore Sigma</td><td>C8661</td><td></td></tr><tr><td>CuSO4 * 5H2O</td><td>Millipore Sigma</td><td>C8027</td><td></td></tr><tr><td>Cysteine-HCl * H2O</td><td>Millipore Sigma</td><td>30129</td><td></td></tr><tr><td>DMSO</td><td>Millipore Sigma</td><td>5439001000</td><td></td></tr><tr><td>DS-11+ Spectrophotometer</td><td>Denovix</td><td>N/A</td><td></td></tr><tr><td>EC-Lab Software</td><td>BioLogic</td><td>N/A</td><td></td></tr><tr><td>ECO E 4 S heating circulator</td><td>Lauda-Brinkmann</td><td>Cat. No. 115 V; 60 Hz : L001191</td><td></td></tr><tr><td>FeSO4 * 7H2O</td><td>Millipore Sigma</td><td>215422</td><td></td></tr><tr><td>Folic acid</td><td>Millipore Sigma</td><td>F8758</td><td></td></tr><tr><td>H3BO3</td><td>Millipore Sigma</td><td>B6768</td><td></td></tr><tr><td>Insulin syringes with BD Micro-Fine IV Needle</td><td>Thermo Fisher Scientific</td><td>14-829-1A</td><td></td></tr><tr><td>Lactiplantibacillus plantarum NCIMB8826</td><td>N/A</td><td>N/A</td><td>Referentie: Tejedor-Sanz et al., 2022</td></tr><tr><td>Lactobacillus MRS Broth</td><td>HiMedia</td><td>M369</td><td></td></tr><tr><td>M9 Broth</td><td>Milliport Sigma</td><td>63011</td><td></td></tr><tr><td>Magnesium sulfate anhydrous</td><td>Millipore Sigma</td><td>208094</td><td></td></tr><tr><td>Manganese sulfate monohydrate</td><td>Millipore Sigma</td><td>221287</td><td></td></tr><tr><td>mannitol</td><td>Millipore Sigma</td><td>M1902-1KG</td><td></td></tr><tr><td>Mettler Toledo FiveEasy Benchtop pH Meter</td><td>Hogentogler</td><td>F20-KIT</td><td></td></tr><tr><td>MgCl2 * 6H2O</td><td>Millipore Sigma</td><td>M9272</td><td></td></tr><tr><td>MgSO4 * 7H2O</td><td>Millipore Sigma</td><td>M2773</td><td></td></tr><tr><td>Millex - GV 0.22 &micro;m PVDF Membrane Filter Unit</td><td>Millipore Sigma</td><td>SLGV004SL</td><td></td></tr><tr><td>MnCl2 * 4H2O</td><td>Millipore Sigma</td><td>203734</td><td></td></tr><tr><td>MnSO4 * H2O</td><td>Millipore Sigma</td><td>221287</td><td></td></tr><tr><td>MOPS</td><td>Millipore Sigma</td><td>M1442</td><td></td></tr><tr><td>N2 gas</td><td>Airgas</td><td>NI UHP300</td><td>Filter voor gebruik</td></tr><tr><td>Na2MoO4 * 2H2O</td><td>Millipore Sigma</td><td>331058</td><td></td></tr><tr><td>Na2SO4</td><td>Millipore Sigma</td><td>238597</td><td></td></tr><tr><td>NaCl</td><td>Millipore Sigma</td><td>S9888</td><td></td></tr><tr><td>NH4Cl</td><td>Millipore Sigma</td><td>A9434</td><td></td></tr><tr><td>Nicotinic acid</td><td>Millipore Sigma</td><td>N-0761</td><td></td></tr><tr><td>Nitrilotriacetic acid (NTA)</td><td>Millipore Sigma</td><td>72560</td><td></td></tr><tr><td>p-Aminobenzoic acid</td><td>Millipore Sigma</td><td>P9879</td><td></td></tr><tr><td>Phosphate buffered saline, 10x solution</td><td>Thermo Fisher Scientific</td><td>BP399-1</td><td></td></tr><tr><td>Potassium phosphate dibasic</td><td>Millipore Sigma</td><td>P8281</td><td></td></tr><tr><td>potentiostat</td><td>BioLogic</td><td>VMP-300</td><td></td></tr><tr><td>Protease peptone #3</td><td>Bacto</td><td>211693</td><td></td></tr><tr><td>Pyridoxine HCl</td><td>Millipore Sigma</td><td>P6280</td><td></td></tr><tr><td>Riboflavin</td><td>Millipore Sigma</td><td>555682</td><td></td></tr><tr><td>RO10 magnetic stir bar platform</td><td>IKA</td><td>3691000</td><td></td></tr><tr><td>Sodium acetate trihydrate</td><td>Millipore Sigma</td><td>935700</td><td></td></tr><tr><td>Stir bar, egg-shaped</td><td>Thermo Fisher Scientific</td><td>14-512-121</td><td>Plaatsen in anodekamer van BES</td></tr><tr><td>Thiamine HCl</td><td>Millipore Sigma</td><td>V-014</td><td></td></tr><tr><td>Thioctic acid (&alpha;-Lipoic acid)</td><td>Millipore Sigma</td><td>T-1395</td><td>

karakterisering van gemedieerde extracellulaire elektronenoverdracht in melkzuurbacteriën met een bio-elektrochemisch systeem met drie elektroden en twee kamers

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Karakterisering van gemedieerde extracellulaire elektronenoverdracht in melkzuurbacteriën met een bio-elektrochemisch systeem met drie elektroden en twee kamers

Many bacteria perform extracellular electron transfer (EET), whereby electrons are transferred from the cell to an extracellular terminal electron ...

characterizing-mediated-extracellular-electron-transfer-lactic-acid

Research

JoVE Journal

Biochemistry

Biochemie

Source: Okamoto, A., et al. Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site. J. Vis. Exp. (2018)This video demonstrates the use of an on-site electrochemical system to enrich anode-respiring bacteria (ARB) from natural reservoirs. A redox gradient is established by placing the anode in oxygen-free deep water and the cathode in oxygen-rich surface water. ARB form biofilms and transfer electrons to the anode, which flows to the cathode, where oxygen is reduced,...

Enrichment of Anode-Respiring Bacteria Using an On-Site Electrochemical System

Source: Tokunou, Y. et. al., Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (2018)This video demonstrates the assembly and operation of an electrochemical reactor model designed to simulate biofilm formation on electrode surfaces. Controlled electrochemical stimulation promotes microbial adhesion, leading to the gradual development of a bacterial monolayer biofilm.

Developing a Bacterial Monolayer Biofilm Using an Electrochemical Reactor

Chronoamperometrische groei van anodisch elektroactieve microbiële biofilms in een fed-batch reactor met behulp van een drie-elektrode opstelling, gecontroleerd door een potentiostaat, wordt gedemonstreerd. De extracellulaire elektronenoverdracht wordt gekenmerkt door middel van cyclische voltammetrie in de aanwezigheid van de elektronendonor en in de afwezigheid van de elektronendonor. Fundamentele data-analyse wordt gedemonstreerd.

Afvalwater Afgeleide Elektroactieve Microbiële Biofilms: Groei, Onderhoud en Basis Karakterisering

We demonstreren de extractie van ammonium uit een ammonium-rijke stroom met behulp van een elektrochemisch en een bio-elektrochemisch systeem. De reactoropstelling, werking en gegevensanalyse worden besproken.

Elektrochemisch en Bioelectrochemically Induced Ammonium Recovery

Een protocol voor het meten van de elektrische geleiding van levende micro-organismen biofilms fysiologisch relevante voorwaarden wordt gepresenteerd.

Karakterisering van elektronentransport via levende Biofilms

Hier presenteren we een protocol van geheel-cel elektrochemische experimenten te bestuderen van de bijdrage van proton vervoer aan het tarief van extracellulaire elektronentransport via de buitenmembraan cytochromes complex in Shewanella oneidensis heer-1.

Elektrochemische detectie van Deuterium kinetisch-isotoopeffect op extracellulaire elektronentransport in Shewanella oneidensis heer-1

On-site microbiële verrijking of in situ -teelttechnieken kunnen het vergemakkelijken van het isolement van de microbiële taxa moeilijk-aan-cultuur, vooral uit laag-biomassa of geochemically extreme omgevingen. Hier beschrijven we een elektrochemische opstelling zonder gebruik te maken van een externe krachtbron te verrijken van microbiële stammen die kunnen extracellulaire elektronentransport (EET).

Zichzelf staande elektrochemische Set-up te verrijken Anode-respiring bacteriën ter plaatse

Hier presenteren we een protocol voor het gebruik van organische elektrochemische transistors (OECT's) om extracellulaire elektronenoverdracht (EET) activiteit in Shewanella oneidensis te vertalen in elektrische signalen. Het hybride OECT-systeem biedt verbeterde robuustheid, gevoeligheid en potentieel voor snelle tests met hoge doorvoer, waardoor het een effectief hulpmiddel is voor EET-metingen.

Vertalen van extracellulaire elektronenoverdrachtsactiviteiten met organische elektrochemische transistors

De hier beschreven protocollen vergemakkelijken de gemakkelijke onderzoeking van bacteriële ethyleenreacties door gebruik te maken van 2-chloorethylfosfonzuur (CEPA).  Ethyleen wordt in situ geproduceerd door de ontbinding van CEPA in een aqueus bacteriegroeimedium, waardoor de vereiste voor zuiver ethyleengas wordt omzeild.

Gebruik makend van de Ethyleen-vrijmakende verbinding, 2-Chloroethylphosphonic zuur, als een instrument om ethyleenreactie in Bacteria Studie

Biopanning bacteriële display bibliotheken is een bewezen techniek voor ontdekking van peptide affiniteit reagentia, een robuust alternatief voor antilichamen. De semi-automatische sorteermethode hierin heeft gestroomlijnd biopanning als u wilt verkleinen van het optreden van valse positieven. We illustreren hier het denkproces en de technieken toegepast in de beoordeling van kandidaten en het minimaliseren van de downstream-analyse.

Semi-automatische Biopanning van bacteriële Display bibliotheken voor Peptide affiniteit reagens detectie en analyse van de resulterende isolaten

Ontdek hoe gemedieerde extracellulaire elektronenoverdracht in Lactiplantibacillus plantarum wordt gekarakteriseerd met een bio-elektrochemisch systeem met drie elektroden en twee kamers.

Karakterisering van gemedieerde extracellulaire elektronenoverdracht in melkzuurbacteriën met een bio-elektrochemisch systeem met drie elektroden en twee kamers (Video) | JoVE Journal

Karakterisering van gemedieerde extracellulaire elektronenoverdracht in melkzuurbacteriën met een bio-elektrochemisch systeem met drie elektroden en twee kamers

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