Name: Celtransplantatie met hoge resolutie bij embryonale en larvale zebravissen (Video) | JoVE Journal
Uploaded: 2024-07-05T15:00:00.000+00:00
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Description: Leer celtransplantatie met hoge resolutie bij embryonale en larvale zebravissen. Deze methode maakt het mogelijk om gedifferentieerde neuronen te transplanteren en ruimtelijke en temporele signaaldynamiek te bestuderen.

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<meta charset="utf8"></head><body>Robuuste zebravisceltransplantatie voor regeneratie- en ontwikkelingsonderzoek

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celtransplantatie met hoge resolutie bij embryonale en larvale zebravissen

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Celtransplantatie met hoge resolutie bij embryonale en larvale zebravissen

Development and regeneration occur by a process of genetically encoded spatiotemporally dynamic cellular interactions. The use of cell transplantation ...

high-resolution-cell-transplantation-in-embryonic-and-larval-zebrafish

Research

JoVE Journal

Developmental Biology

Ontwikkelingsbiologie

Zebravis celtransplantatie maakt de combinatie van genetica en embryologie aan het weefsel specifieke hersenschimmen te genereren. Deze video toont gastrula geënsceneerde cel transplantaties die hebben onze lab naar de rol van astroglial bevolking en de specifieke begeleiding signalen tijdens commissuur vorming in de voorhersenen te onderzoeken.

Tissue Gerichte Embryonale Chimera: Zebravis gastrula Cell Transplantation

Demonstreren we een protocol om chimere zebravis embryo's te genereren voor live-imaging cellulaire gedrag tijdens de embryogenese.

Transplantatie van GFP-expressie blastomeren voor Live Beeldvorming van het netvlies en de ontwikkeling van de hersenen in chimère zebravis embryo&#39;s

De zebravis, een kleine tropische vissen, is uitgegroeid tot een populair model voor het bestuderen van gen-functie tijdens gewervelde ontwikkeling en ziekte. De ruimtelijke en temporele expressie van de doelwitgenen worden...

Hoge resolutie Hele Mount<em&gt; In Situ</em&gt; Hybridisatie in zebravis embryo&#39;s om genexpressie en functie te bestuderen

Hier presenteren we een protocol voor celtransplantatie van zebravis skeletspier en embryonaal rabdomyosarcoom (ERMS) in volwassen immuungecompromitteerde rag2E450fs homozygoot mutante zebravissen. Dit protocol maakt een efficiënte analyse van regeneratie en maligne transformatie van spiercellen mogelijk.

Normale en maligne Muscle Cell Transplantation in immuungecompromiteerde Adult zebravis

Dit protocol beschrijft een methode voor het isoleren van enkele cellen uit zebravis-embryo's, het verrijken van cellen van belang, het vastleggen van zebraviscellen in microfluïdische gebaseerde enkelcellige multiplexsystemen en het beoordelen van genexpressie uit enkele cellen.

Isolatie en karakterisering van enkele cellen van zebravis embryo&#39;s

Live volgen van individuele WT retinale voorlopers in verschillende genetische achtergronden maakt voor de beoordeling van de bijdrage van de cel niet-zelfstandige signalering tijdens de neurogenese. Hier, een combinatie van gen knockdown, chimeer generatie via embryo transplantatie en in vivo time-lapse confocale beeldvorming werd gebruikt voor dit doel.

In vivo beeldvorming van transgene Gene Expression Individuele retinale voorouders in chimère zebravis embryo&#39;s to Cell Nonautonomous Invloeden Studie

Presenteren we een efficiënt en gemakkelijk te gebruiken protocol voor het voorbereiden van primaire celculturen van zebravis embryo's voor transfectie en levende cel imaging, evenals een protocol bij het bereiden van primaire cellen van volwassen zebrafish hersenen.

Cultuur en de transfectie van zebravis primaire cellen

Embryologische manipulaties zoals uitroeiing en transplantatie van cellen zijn belangrijke hulpmiddelen om vroege ontwikkeling te bestuderen. Dit protocol beschrijft een eenvoudig en effectief transplantatieapparaat om deze manipulaties uit te voeren in zebravisembryo's.

Een eenvoudig en effectief transplantatieapparaat voor zebravisembryo's

Dit werk beschrijft een protocol voor het modulaire Tol2-transgenesesysteem, een gateway-gebaseerde kloonmethode om transgene constructies in zebravisembryo's te creëren en te injecteren.

Het Zebrafish Tol2-systeem: een modulaire en flexibele gateway-gebaseerde transgenesebenadering

We beschrijven een protocol voor xenotransplantatie in de dooier van transparante zebravisembryo's dat wordt geoptimaliseerd door een eenvoudige, snelle stadiëringsmethode. Analyses na injectie omvatten overleving en het beoordelen van de ziektelast van xenogetransplanteerde cellen door middel van flowcytometrie.

Een eenvoudige, snelle en effectieve methode voor tumor xenotransplantatie analyse in transparante zebravis embryo's

Leer celtransplantatie met hoge resolutie bij embryonale en larvale zebravissen. Deze methode maakt het mogelijk om gedifferentieerde neuronen te transplanteren en ruimtelijke en temporele signaaldynamiek te bestuderen.

Celtransplantatie met hoge resolutie bij embryonale en larvale zebravissen

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