Name: Uitgebreide karakterisering van weefselmineralisatie in een ex vivo model (Video) | JoVE Journal
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Description: Leer weefselmineralisatie karakteriseren in een ex vivo model voor botregeneratie met multimodale technieken. Deze methode helpt onderzoekers bij het vroegtijdig identificeren van veelbelovende kandidaten voor therapeutische toepassingen.

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<meta charset="utf8"></head><body>Multimodale analyse van botvorming met behulp van explantatiemodellen

<ol><li>Feroz, S., Cathro, P., Ivanovski, S., Muhammad, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biomimetic+bone+grafts+and+substitutes%3A+A+review+of+recent+advancements+and+applications%5BTitle%5D)+AND+%22Biomed+Eng+Adv%22%5BJournal%5D)">Biomimetic bone grafts and substitutes: A review of recent advancements and applications.</a> Biomed Eng Adv. 6, 100107(2023).</li>
<li>Tsuji, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((BMP2+activity%2C+although+dispensable+for+bone+formation%2C+is+required+for+the+initiation+of+fracture+healing%5BTitle%5D)+AND+%22Nat+Genet%22%5BJournal%5D)">BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing.</a> Nat Genet. 38 (12), 1424-1429 (2006).</li>
<li>Hadjidakis, D. J., Androulakis, I. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Boneremodeling%5BTitle%5D)+AND+%22Ann+N+Y+Acad+Sci%22%5BJournal%5D)">Boneremodeling.</a> Ann N Y Acad Sci. 1092, 385-396 (2006).</li>
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<li>Boivin, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+remodeling+on+the+mineralization+of+bone+tissue%5BTitle%5D)+AND+%22Osteoporo+Int%22%5BJournal%5D)">Influence of remodeling on the mineralization of bone tissue.</a> Osteoporo Int. 20 (6), 1023-1026 (2009).</li>
<li>Zanghellini, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multimodal+analysis+and+comparison+of+stoichiometric+and+structural+characteristics+of+parosteal+and+conventional+osteosarcoma+with+massive+sclerosis+in+human+bone%5BTitle%5D)+AND+%22J+Str+Biol%22%5BJournal%5D)">Multimodal analysis and comparison of stoichiometric and structural characteristics of parosteal and conventional osteosarcoma with massive sclerosis in human bone.</a> J Str Biol. 216 (3), 108106(2024).</li>
<li>Trento, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((formation+around+two+titanium+implant+surfaces+placed+in+bone+defects+with+and+without+a+bone+substitute+material%3A+A+histological%2C+histomorphometric%2C+and+micro-computed+tomography+evaluation%5BTitle%5D)+AND+%22Clin+Implant+Dent+Relat+Res%22%5BJournal%5D)">formation around two titanium implant surfaces placed in bone defects with and without a bone substitute material: A histological, histomorphometric, and micro-computed tomography evaluation.</a> Clin Implant Dent Relat Res. 22 (2), 177-185 (2020).</li>
<li>Palmquist, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+multiscale+analytical+approach+to+evaluate+osseointegration%5BTitle%5D)+AND+%22J+Mater+Sci+Mater+Med%22%5BJournal%5D)">A multiscale analytical approach to evaluate osseointegration.</a> J Mater Sci Mater Med. 29 (5), 60(2018).</li>
<li>Budán, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Novel+radiomics+evaluation+of+bone+formation+utilizing+multimodal+%28SPECT%2FX-ray+CT%29+in+vivo+imaging%5BTitle%5D)+AND+%22PLoS+ONE%22%5BJournal%5D)">Novel radiomics evaluation of bone formation utilizing multimodal (SPECT/X-ray CT) in vivo imaging.</a> PLoS ONE. 13 (9), e0204423(2018).</li>
<li>Hulsart-Billström, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+surprisingly+poor+correlation+between+in+vitro+and+in+vivo+testing+of+biomaterials+for+bone+regeneration%3A+results+of+a+multicentre+analysis%5BTitle%5D)+AND+%22Eur+Cells+Mater%22%5BJournal%5D)">A surprisingly poor correlation between in vitro and in vivo testing of biomaterials for bone regeneration: results of a multicentre analysis.</a> Eur Cells Mater. 31, 312-322 (2016).</li>
<li>Cramer, E. E. A., Ito, K., Hofmann, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ex+vivo+bone+models+and+their+potential+in+preclinical+evaluation%5BTitle%5D)+AND+%22Curr+Osteoporo+Rep%22%5BJournal%5D)">Ex vivo bone models and their potential in preclinical evaluation.</a> Curr Osteoporo Rep. 19 (1), 75-87 (2021).</li>
<li>Chan, M. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+trabecular+bone+explant+model+of+osteocyte-osteoblast+co-culture+for+bone+mechanobiology%5BTitle%5D)+AND+%22Cell+Mol+Bioeng%22%5BJournal%5D)">A trabecular bone explant model of osteocyte-osteoblast co-culture for bone mechanobiology.</a> Cell Mol Bioeng. 2 (3), 405-415 (2009).</li>
<li>Doke, S. K., Dhawale, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Alternatives+to+animal+testing%3A+A+review%5BTitle%5D)+AND+%22Saudi+Pharma+J%22%5BJournal%5D)">Alternatives to animal testing: A review.</a> Saudi Pharma J. 23 (3), 223-229 (2015).</li>
<li>Moussi, H., et al. New formulation of injectable and degradable calcium phosphate/silanized hyaluronic acid composite foam: Investigation in a rabbit model of long bone defect. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aMoussi%2C+H+%22SSRN%22">SSRN</a>. , (2024).</li>
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<li>Roschger, P., Fratzl, P., Eschberger, J., Klaushofer, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+quantitative+backscattered+electron+imaging+for+the+measurement+of+mineral+density+distribution+in+human+bone+biopsies%5BTitle%5D)+AND+%22Bone%22%5BJournal%5D)">Validation of quantitative backscattered electron imaging for the measurement of mineral density distribution in human bone biopsies.</a> Bone. 23 (4), 319-326 (1998).</li>
<li>Roschger, P., Plenk, H., Klaushofer, K., Eschberger, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+new+scanning+electron+microscopy+approach+to+the+quantification+of+bone+mineral+distribution%3A+backscattered+electron+image+grey-levels+correlated+to+calcium+K+alpha-line+intensities%5BTitle%5D)+AND+%22Scanning+Micro%22%5BJournal%5D)">A new scanning electron microscopy approach to the quantification of bone mineral distribution: backscattered electron image grey-levels correlated to calcium K alpha-line intensities.</a> Scanning Micro. 9 (1), 75-86 (1995).</li>
<li>Porter, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quick+and+inexpensive+paraffin-embedding+method+for+dynamic+bone+formation+analyses%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Quick and inexpensive paraffin-embedding method for dynamic bone formation analyses.</a> Sci Rep. 7, 42505(2017).</li>
<li>Goldschlager, T., Abdelkader, A., Kerr, J., Boundy, I., Jenkin, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Undecalcified+bone+preparation+for+histology%2C+histomorphometry+and+fluorochrome+analysis%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Undecalcified bone preparation for histology, histomorphometry and fluorochrome analysis.</a> J Vis Exp. (35), e1707(2010).</li>
<li>Kazanci, M., Roschger, P., Paschalis, E. P., Klaushofer, K., Fratzl, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bone+osteonal+tissues+by+Raman+spectral+mapping%3A+Orientation-composition%5BTitle%5D)+AND+%22J+Str+Biol%22%5BJournal%5D)">Bone osteonal tissues by Raman spectral mapping: Orientation-composition.</a> J Str Biol. 156 (3), 489-496 (2006).</li>
<li>Unal, M., Ahmed, R., Mahadevan-Jansen, A., Nyman, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Compositional+assessment+of+bone+by+Raman+spectroscopy%5BTitle%5D)+AND+%22Analyst%22%5BJournal%5D)">Compositional assessment of bone by Raman spectroscopy.</a> Analyst. 146 (24), 7464-7490 (2021).</li>
<li>Oliver, W. C., Pharr, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+improved+technique+for+determining+hardness+and+elastic+modulus+using+load+and+displacement+sensing+indentation+experiments%5BTitle%5D)+AND+%22J+Mater+Res%22%5BJournal%5D)">An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments.</a> J Mater Res. 7 (6), 1564-1583 (1992).</li>
<li>Proffen, B. L., McElfresh, M., Fleming, B. C., Murray, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+comparative+anatomical+study+of+the+human+knee+and+six+animal+species%5BTitle%5D)+AND+%22Knee%22%5BJournal%5D)">A comparative anatomical study of the human knee and six animal species.</a> Knee. 19 (4), 493-499 (2012).</li>
<li>Allen, M. J., Houlton, J. E. F., Adams, S. B., Rushton, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+surgical+anatomy+of+the+stifle+joint+in+Sheep%5BTitle%5D)+AND+%22Vet+Surg%22%5BJournal%5D)">The surgical anatomy of the stifle joint in Sheep.</a> Vet Surg. 27 (6), 596-605 (1998).</li>
<li>Kleuskens, M. W. A., van Donkelaar, C. C., Kock, L. M., Janssen, R. P. A., Ito, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+ex+vivo+human+osteochondral+culture+model%5BTitle%5D)+AND+%22J+Ortho+Res%22%5BJournal%5D)">An ex vivo human osteochondral culture model.</a> J Ortho Res. 39 (4), 871-879 (2021).</li>
</ol>

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uitgebreide karakterisering van weefselmineralisatie in een ex vivo model

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Uitgebreide karakterisering van weefselmineralisatie in een ex vivo model

The extensive characterization of tissue mineralization in the context of bone regeneration represents a significant challenge, given the numerous ...

comprehensive-characterization-tissue-mineralization-an-ex-vivo

Research

JoVE Journal

Bioengineering

Bio-engineering

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Diermodellen worden vaak gebruikt om uitzaaiing studeren bot. In dit protocol beschrijven we twee gebruikelijke methoden van tumorinoculatie voor bot metastase studies en beschrijf kort enkele van de analyses gebruikt om toezicht te houden en te kwantificeren deze modellen.

Modellen van bot metastase

In this video, we describe a method for the detection of osteoblast-secreted calcium deposits using an anionic dye, Alizarin Red S. The cultured osteoblasts are first stained and then the dye is recovered for colorimetric quantification of calcium deposits or the degree of osteoblast mineralization.

In Vitro Mineralization Assay: A Colorimetric Method to Quantify Osteoblast Calcium Deposits on Bone Graft Substitute by Alizarin Red S Staining and Extraction

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High-throughput, Multi-Beeld Cryohistology gemineraliseerde weefsels

We presenteren een protocol om te vergelijken de status van mineralen in blaasjes uitgebracht door twee menselijk bot cellijnen: hFOB 1.19 en Saos-2. Hun mineralisatie profielen werden geanalyseerd door Alizarine Red-S (S-AR) kleuring, ultraviolet (UV) licht visualisatie, Transmissie Electronenmicroscopie (TEM) beeldvorming en energie dispersieve x-stralen Microanalyse (EDX).

Analyse van mineralen geproduceerd door hFOB 1.19 en Saos-2 cellen met behulp van Transmissie Electronenmicroscopie met energie dispersieve x-stralen Microanalyse

Hart- en vaatziekten zijn wereldwijd de belangrijkste doodsoorzaak. Vasculaire verkalking draagt substantieel bij aan de last van cardiovasculaire morbiditeit en mortaliteit. Dit protocol beschrijft een eenvoudige methode om vasculaire gladde spiercel-gemedieerde calciumprecipitatie in vitro te kwantificeren door fluorescentie beeldvorming.

Een semi-geautomatiseerde en reproduceerbare biologische methode om calciumdepositie in vitro te kwantificeren

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Een menselijk beenmerg 3D-model om de dynamiek en interacties tussen residente cellen in fysiologische of tumorale contexten te onderzoeken

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Evaluatie van de microarchitectuur van het trabeculaire bot in een muismodel met osteoporose

We stellen een protocol voor voor het beoordelen van botregeneratie op verschillende hiërarchische niveaus. Deze multimodale aanpak maakt structurele analyse mogelijk over verschillende lengteschalen, waardoor uitdagingen bij de monstervoorbereiding effectief worden aangepakt. Het biedt ook een betrouwbare workflow voor het evalueren van de effectiviteit van biomaterialen bij het bevorderen van botregeneratie.

Multimodale benadering om botregeneratie en steigerprestaties te beoordelen

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Een 3D-botkweekplatform met menselijke osteocyten en gedecellulariseerde extracellulaire matrix voor het modelleren van musculoskeletale aandoeningen

Leer weefselmineralisatie karakteriseren in een ex vivo model voor botregeneratie met multimodale technieken. Deze methode helpt onderzoekers bij het vroegtijdig identificeren van veelbelovende kandidaten voor therapeutische toepassingen.

Uitgebreide karakterisering van weefselmineralisatie in een ex vivo model

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