Name: Multimodale studie van cardiovasculaire remodellering bij muizen: vierdimensionale echografie en massaspectrometriebeeldvorming (Video) | JoVE Journal
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Description: Ontdek vierdimensionale echografie en massaspectrometriebeeldvorming voor cardiovasculaire remodellering bij muizen. Deze technieken combineren functionele en moleculaire analyse voor multiomisch onderzoek naar hartinfarct en veroudering.

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<meta charset="utf8"></head><body>4D-echografie en MALDI-beeldvorming voor hart- en vaatanalyse bij muizen

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<li>Salehi, H. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coregistered+photoacoustic+and+ultrasound+imaging+and+classification+of+ovarian+cancer%3A+Ex+vivo+and+in+vivo+studies%5BTitle%5D)+AND+%22J+Biomed+Opt%22%5BJournal%5D)">Coregistered photoacoustic and ultrasound imaging and classification of ovarian cancer: Ex vivo and in vivo studies.</a> J Biomed Opt. 21 (4), 046006(2016).</li>
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<li>Yang, E. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Real-time+co-registration+using+novel+ultrasound+technology%3A+Ex+vivo+validation+and+in+vivo+applications%5BTitle%5D)+AND+%22J+Am+Soc+Echocardiogr%22%5BJournal%5D)">Real-time co-registration using novel ultrasound technology: Ex vivo validation and in vivo applications.</a> J Am Soc Echocardiogr. 24 (7), 720(2011).</li>
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</ol>

<table><tbody><tr><td>2,5-dihydroxybenzoic acid (DHB)</td><td>Supelco,  >99.0% (HPLC)</td><td>85707-10MG-F</td><td>DHB matrixsubstance voor MALDI-MS; https://www.sigmaaldrich.com/US/en/product/sigma/85707?cm_sp=Insite-_-wimsShippingEmailRecs_wims EmailAPI_wimsGruCrossEntropy-_-wimsEmailAPI10-3</td></tr><tr><td>9-aminoacridine (9AA)</td><td>Supelco,  &ge;99.5% (HPLC)</td><td>92817-1G</td><td>9-Aminoacridine matrixsubstance voor MALDI-MS; https://www.sigmaaldrich.com/US/en/product/sial/92817?srsltid=AfmBOooiQjQ4pWv_XxITkU 4Lkm0UnHXKekGS_ dFl7V40V9QLWoPpNLoc</td></tr><tr><td>Aquasonic Ultrasound Gel</td><td>Parker Laboratories</td><td>Parker 01-02</td><td>Ultrasound Gel; https://www.parkerlabs.com/products/aquasonic-100-ultrasound-transmission-gel/</td></tr><tr><td>Benchtop Dewar Flasks</td><td>ThermoScientific&nbsp;</td><td>4150-2000</td><td>Container voor vloeibare stikstof; https://www.thermofisher.com/order/catalog/product/4150-4000?gclid=Cj0KCQjwpvK4BhDUARIsA DHt9sQVc2f-NxN04Nb5Mv F6TZ7GLHWWDEeqDYmEvtKJSQ YHDeVgZ9qylvYaAs27EALw_wcB &amp;source=google_shopping&amp;ISO_ CODE=us&amp;LANG_CODE=en&amp;ef_id =Cj0KCQjwpvK4BhDUARIsADHt9 sQVc2f-NxN04Nb5MvF6TZ7GLHWWDE eqDYmEvtKJSQYHDeVgZ9qylvYa As27EALw_wcB:G:s&amp;s_kwcid=AL!3652 !3!716188292869!!!g!2366243726129 !!21787513085!171591181194&amp;ev_chn =shop&amp;cid=0se_gaw_30092024_ PBYTXL&amp;source=google_shopping &amp;ISO_CODE=us&amp;LANG_CODE= en&amp;gad_source=1</td></tr><tr><td>Cryostat</td><td>Leica Biosystems</td><td>CM Series</td><td>https://www.leicabiosystems.com/us/histology-equipment/cryostats/</td></tr><tr><td>Dessicator</td><td>VWR&nbsp;</td><td>89054-052</td><td>https://us.vwr.com/store/product/9104882/desiccator-plastic-ace-glass-incorporated</td></tr><tr><td>Epredia MX35 Premier Disposable Low-profile&nbsp;Microtome&nbsp;Blades</td><td>Fisher Scientific</td><td>3052835</td><td>Cryostat blade; https://www.fishersci.com/shop/products/mx35-premier-disposable-low-profile-microtome-blades/3052835</td></tr><tr><td>Falcon 15 mL Conical Centrifuge Tubes</td><td>Fisher Scientific</td><td>14-959-53A</td><td>Conical Tubes; https://www.fishersci.com/shop/products/falcon-15ml-conical-centrifuge-tubes-5/1495953A?gclid=Cj0KCQjwpvK4BhDUARIsA DHt9sSBcy5n-lhShligJUOX5KKVGn0bt87 8AB2_muOD2PPTue1phpZgeqwa AqgiEALw_wcB&amp;ef_id=Cj0KCQjw pvK4BhDUARIsADHt9sSBcy5n-lhS hligJUOX5KKVGn0bt878AB2_muO D2PPTue1phpZgeqwaAqgiEALw_ wcB:G:s&amp;ppc_id=PLA_goog_20861 45674_81843405034_1495953A__ 386247001345_165426395473886 37329&amp;ev_chn=shop&amp;s_kwcid=AL!4428!3 !386247001345!!!g!856907751004!&amp; gad_source=1</td></tr><tr><td>Flex-Tubes Microcentrifuge Tubes</td><td>Eppendorf&nbsp;</td><td>EP022364120</td><td>Centrifuge tubes; https://www.sigmaaldrich.com/US/en/product/sigma/ep022364120?utm_source=google%2Cgoogle&amp;utm _medium=organicshopping%2Ccpc&amp;utm _campaign=21043330280&amp;utm_ content=&amp;gclid=Cj0KCQjwpv K4BhDUARIsADHt9sTHRD35k CHPtfI2A41axodnMVr6a1eBKk zM4bSUYQAyfEKo3UgTAEQa Ap7wEALw_wcB</td></tr><tr><td>Gas Nitrogen</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Glass microscope slides</td><td>Electron Microscopy Sciences</td><td>71873-02</td><td>https://www.emsdiasum.com/positive-charge-microscope-slides</td></tr><tr><td>Liquid Nitrogen</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Mass Spectrometer</td><td>Waters</td><td>Synapt G2-Si</td><td>https://www.waters.com/waters/en_US/SYNAPT-G2-Si-Mass-Spectrometry/nav.htm?locale=en_mkcid=1000251Foodety%3C/a%3E&amp;cid=134740653&amp;bcid= 134528734</td></tr><tr><td>Matrix Sprayer</td><td>HTX Technologies</td><td>M3+</td><td>https://www.htximaging.com/htx-m3-sprayer</td></tr><tr><td>Methanol (HPLC), >99.9%</td><td>Fisher Chemical&nbsp;</td><td>A4524</td><td>Methanol; https://www.fishersci.com/shop/products/methanol-hplc-fisher-chemical-9/A4524?crossRef=A4524#?keyword=A4524</td></tr><tr><td>Preclinical Ultrasound System&nbsp;</td><td>FUJIFILM VisualSonics</td><td>Vevo 3100</td><td>https://www.visualsonics.com/product/imaging-systems/vevo-3100; Vevo F2 heeft de Vevo 3100 in productie vervangen. Systeem bevat isofluraan verdamper en inductiebox.&nbsp;</td></tr><tr><td>Reynolds Wrap</td><td>N/A</td>

multimodale studie van cardiovasculaire remodellering bij muizen: vierdimensionale echografie en massaspectrometriebeeldvorming

Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging

Multimodale studie van cardiovasculaire remodellering bij muizen: vierdimensionale echografie en massaspectrometriebeeldvorming

Cardiovascular disease (CVD) is the leading cause of death in the United States. Damage in&#160;the cardiovascular system can be due to environmental ...

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Bioengineering

Bio-engineering

Deze video demonstreert het gebruik van een rail gemonteerd hoogfrequente ultrasone sonde naar echocardiografie te voeren op een verdoofde muis. De methoden beschrijven zowel conventionele twee-dimensionale en M-mode metingen van de hartfunctie in aanvulling op nieuwere, krachtige tools zoals kleur Doppler, rek-analyse, maar ook algemene en gerichte contrast beeldvorming.

Muriene Echocardiografie en Ultrasound Imaging

Echocardiografie wordt vaak gebruikt om veranderingen in de hartstructuur en -functie niet-invasief te karakteriseren en te kwantificeren. We beschrijven een op echografie gebaseerd beeldalgoritme dat een verbeterde surrogaatmeting van myocardmicrostructuur biedt en kan worden uitgevoerd met behulp van open-access beeldanalysesoftware.

Ultrasone beoordeling van myocard microstructuur

Het doel van dit protocol is om niet-invasief beoordelen cardiale structurele en functionele veranderingen in een muismodel van hart-en vaatziekten door dwarse aorta vernauwing, met behulp van B- en M-mode echocardiografie en kleur / pulse wave Doppler imaging.

Beoordeling van Cardiac morfologische en functionele veranderingen in muismodel van Transversale Aorta Vernauwing door Echocardiografie Imaging

Dit protocol geeft een gedetailleerde beschrijving van de echocardiografische benadering voor uitgebreide fenotypering van de hart- en hartklepfunctie bij muizen.

Echocardiografische benaderingen en protocollen voor Comprehensive Fenotypische karakterisering van Valvular hart-en vaatziekten in Muizen

Echografie imaging is geworden een gemeenschappelijke modaliteit te bepalen de luminal afmetingen van borst- en buikholte aorta aneurysma in muizen. Dit protocol beschrijft de procedure om het verkrijgen van betrouwbare en reproduceerbare tweedimensionale ultrasound beelden van de oplopende en abdominale aorta in muizen.

Echografie beeldvorming van de borst- en buikholte Aorta bij muizen om te bepalen van de afmetingen van het aneurysma

Deze studie beschrijft een uitgebreid cardiovasculair magnetisch resonantie beeldvorming (CMR) protocol om de linker ventriculaire functionele parameters van het muishart te kwantificeren. Het protocol beschrijft de verwerving, nabewerking en analyse van de CMR-beelden en de beoordeling van verschillende cardiale functionele parameters.

Kwantificering van de linkerventrikelfunctie van het muishart, myocardiale spanning en hemodynamische krachten door cardiovasculaire magnetische resonantiebeeldvorming

Dit protocol demonstreert robotische echografie (VS) als een praktisch, kosteneffectief en snel alternatief voor traditionele niet-invasieve beeldmodaliteiten.

Gebruik van 3D-robotechografie voor in vivo analyse van muizennieren

We rapporteren een methode voor mesoscopische reconstructie van het hele muizenhart door nieuwe ontwikkelingen in weefseltransformatie en kleuring te combineren met de ontwikkeling van een axiaal gescande lichtplaatmicroscoop.

Mesoscopische optische beeldvorming van het hele muizenhart

Het protocol maakt gebruik van geavanceerde light-sheet microscopie samen met aangepaste methoden voor het opruimen van weefsel om ingewikkelde hartstructuren in knaagdierharten te onderzoeken, met een groot potentieel voor het begrijpen van cardiale morfogenese en remodellering.

Light-sheet beeldvorming om de hartstructuur in knaagdierharten te onthullen

Ultrasnelle Doppler-echografie (UFUS) met een hoge ruimtelijke resolutie (100 μm) en gevoeligheid is een geschikte niet-invasieve beeldvormingsmodaliteit voor het verkrijgen van een uitgebreid kwalitatief overzicht van de levervasculatuur. UFUS faciliteert ook kwantitatieve metingen van de microvasculatuur, met als doel ons begrip van vaatziektemechanismen te vergroten.

Beeldvorming en kwantificering van het levervaatstelsel van muizen met behulp van ultrasnelle Doppler-echografie

Ontdek vierdimensionale echografie en massaspectrometriebeeldvorming voor cardiovasculaire remodellering bij muizen. Deze technieken combineren functionele en moleculaire analyse voor multiomisch onderzoek naar hartinfarct en veroudering.

Multimodale studie van cardiovasculaire remodellering bij muizen: vierdimensionale echografie en massaspectrometriebeeldvorming

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