Name: Efficiënte genknockdown in de lever via intrasplenische injectie van adeno-geassocieerd virus serotype 8 (AAV8)-afgeleverd klein haarspeld-RNA (Video) | JoVE Journal
Uploaded: 2024-11-01T14:50:00.000+00:00
Duration: 4 min 29 s
Description: Leer een eenvoudigere methode voor genknockdown in de lever via intrasplenische injectie van AAV8-afgeleverd klein haarspeld-RNA. Deze techniek biedt gelijke efficiëntie met minder complicaties dan portale veneuze injectie.

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<meta charset="utf8"></head><body>Intrasplenische AAV8-shRNA-injectie voor efficiënte en veilige knockdown van levergen

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<table><tbody><tr><td>Amicon filter centrifugatie-eenheden</td><td>MilliporeSigma</td><td>UFC9100</td><td>Snelle ultrafiltratie</td></tr><tr><td>AV5-siRNA-GFP&nbsp;</td><td>Addgene&nbsp;</td><td>#124972</td><td>Plasmid</td></tr><tr><td>Competente DH5&alpha; bacteriën&nbsp;</td><td>Invitrogen</td><td>#18265-017</td><td>Chemisch competente stam voor klonering&nbsp;</td></tr><tr><td>HpaI&nbsp;</td><td>New England Biolabs</td><td>R0105</td><td>Restrictienezym</td></tr><tr><td>iMFectin transfectie reagens&nbsp;</td><td>GenDepot</td><td>I7100-101</td><td></td></tr><tr><td>M-MLV reverse transcriptase&nbsp;</td><td>Promega</td><td>M1708</td><td>RNA-afhankelijke DNA-polymerase&nbsp;</td></tr><tr><td>MyiQ enkelkleur real-time PCR detectiesysteem&nbsp;</td><td>Bio-Rad</td><td>BUN9740RAD</td><td>qRT-PCR</td></tr><tr><td>Omega endotoxin-vrij kit&nbsp;</td><td>Bioteck</td><td>D6915-03</td><td>Plasmid DNA midi prep&nbsp;</td></tr><tr><td>Willekeurige hexamers&nbsp;</td><td>Invitrogen</td><td>48190-011</td><td></td></tr><tr><td>Supermix SYBR green reagens&nbsp;</td><td>Bio-Rad</td><td>1708882</td><td>Real-time PCR toepassingen</td></tr><tr><td>T4 DNA Ligase</td><td>Promega</td><td>M1801</td><td></td></tr><tr><td>TRIzol Reagent&nbsp;</td><td>Life Technologies</td><td>15596-018</td><td>Isolatie van hoogwaardige totale RNA</td></tr><tr><td>XhoI</td><td>New England Biolabs</td><td>R0146</td><td>Restrictienezym</td></tr></tbody></table>

efficiënte genknockdown in de lever via intrasplenische injectie van adeno-geassocieerd virus serotype 8 (aav8)-afgeleverd klein haarspeld-rna

Efficient Gene Knockdown in the Liver via Intrasplenic Injection of Adeno-Associated Virus Serotype 8 (AAV8)-Delivered Small Hairpin RNA

Efficiënte genknockdown in de lever via intrasplenische injectie van adeno-geassocieerd virus serotype 8 (AAV8)-afgeleverd klein haarspeld-RNA

The liver is a major organ that performs essential metabolic functions. Developing an efficient and safe method to knock down gene expression in the liver ...

efficient-gene-knockdown-liver-via-intrasplenic-injection-adeno

Research

JoVE Journal

Cancer Research

Kankeronderzoek

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RNA-interferentie (RNAi) beschikt over een groot aantal voordelen ten opzichte van gen knock-out en is in brede kring gebruikt als een instrument in gen functionele studies. De uitvinding van DNA-vector-gebaseerde RNAi-technologie heeft op lange termijn en induceerbaar gen knockdown mogelijk, en verhoogde ook de haalbaarheid van gene...

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Effectieve en veilige genafgifte aan de nier van de muis via langzame retrograde injectie van nierbekken van adeno-geassocieerde virusvectoren

Leer een eenvoudigere methode voor genknockdown in de lever via intrasplenische injectie van AAV8-afgeleverd klein haarspeld-RNA. Deze techniek biedt gelijke efficiëntie met minder complicaties dan portale veneuze injectie.

Efficiënte genknockdown in de lever via intrasplenische injectie van adeno-geassocieerd virus serotype 8 (AAV8)-afgeleverd klein haarspeld-RNA (Video) | JoVE Journal

Efficiënte genknockdown in de lever via intrasplenische injectie van adeno-geassocieerd virus serotype 8 (AAV8)-afgeleverd klein haarspeld-RNA

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