Name: Massacytometrie-analyse van systemische en lokale immuunresponsen bij hepatocellulair carcinoom (Video) | JoVE Journal
Uploaded: 2025-04-25T13:00:00.000+00:00
Duration: 8 min 25 s
Description: Leer massacytometrie-analyse van systemische en lokale immuunresponsen bij hepatocellulair carcinoom. Deze methode identificeert 14 celtypen op enkel-celniveau en onthult unieke expressieprofielen voor gedetailleerd immuunonderzoek.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Zhang, C. H., Cheng, Y., Zhang, S., Fan, J., Gao, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Changing+epidemiology+of+hepatocellular+carcinoma+in+Asia%5BTitle%5D)+AND+%22Liver+Int%22%5BJournal%5D)">Changing epidemiology of hepatocellular carcinoma in Asia.</a> Liver Int. 42 (9), 2029-2041 (2022).</li>
<li>Renne, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hepatocellular+carcinoma%3A+A+clinical+and+pathological+overview%5BTitle%5D)+AND+%22Pathologica%22%5BJournal%5D)">Hepatocellular carcinoma: A clinical and pathological overview.</a> Pathologica. 113 (3), 203-217 (2021).</li>
<li>Sayiner, M., Golabi, P., Younossi, Z. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Disease+burden+of+hepatocellular+carcinoma%3A+A+global+perspective%5BTitle%5D)+AND+%22Dig+Dis+Sci%22%5BJournal%5D)">Disease burden of hepatocellular carcinoma: A global perspective.</a> Dig Dis Sci. 64 (4), 910-917 (2019).</li>
<li>Zhang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Risk+factors+and+prevention+of+viral+hepatitis-related+hepatocellular+carcinoma%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Risk factors and prevention of viral hepatitis-related hepatocellular carcinoma.</a> Front Oncol. 11, 686962(2021).</li>
<li>Zou, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Economic+burden+and+quality+of+life+of+hepatocellular+carcinoma+in+greater+China%3A+A+systematic+review%5BTitle%5D)+AND+%22Front+Public+Health%22%5BJournal%5D)">Economic burden and quality of life of hepatocellular carcinoma in greater China: A systematic review.</a> Front Public Health. 10, 801981(2022).</li>
<li>Li, Y., You, Z., Tang, R., Ma, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tissue-resident+memory+t+cells+in+chronic+liver+diseases%3A+Phenotype%2C+development+and+function%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Tissue-resident memory t cells in chronic liver diseases: Phenotype, development and function.</a> Front Immunol. 13, 967055(2022).</li>
<li>Shen, K. Y., Zhu, Y., Xie, S. Z., Qin, L. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunosuppressive+tumor+microenvironment+and+immunotherapy+of+hepatocellular+carcinoma%3A+Current+status+and+prospectives%5BTitle%5D)+AND+%22J+Hematol+Oncol%22%5BJournal%5D)">Immunosuppressive tumor microenvironment and immunotherapy of hepatocellular carcinoma: Current status and prospectives.</a> J Hematol Oncol. 17 (1), 25(2024).</li>
<li>Oura, K., Morishita, A., Tani, J., Masaki, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tumor+immune+microenvironment+and+immunosuppressive+therapy+in+hepatocellular+carcinoma%3A+A+review%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Tumor immune microenvironment and immunosuppressive therapy in hepatocellular carcinoma: A review.</a> Int J Mol Sci. 22 (11), 5801(2021).</li>
<li>Chen, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+infiltrating+immune+cells+in+tumor+microenvironment+on+metastasis+of+hepatocellular+carcinoma%5BTitle%5D)+AND+%22Cell+Oncol%22%5BJournal%5D)">Effect of infiltrating immune cells in tumor microenvironment on metastasis of hepatocellular carcinoma.</a> Cell Oncol. 46 (6), 1595-1604 (2023).</li>
<li>Du, Q., An, Q., Zhang, J., Liu, C., Hu, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Unravelling+immune+microenvironment+features+underlying+tumor+progression+in+the+single-cell+era%5BTitle%5D)+AND+%22Cancer+Cell+Int%22%5BJournal%5D)">Unravelling immune microenvironment features underlying tumor progression in the single-cell era.</a> Cancer Cell Int. 24 (1), 143(2024).</li>
<li>Xu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Reshaping+the+systemic+tumor+immune+environment+%28stie%29+and+tumor+immune+microenvironment+%28time%29+to+enhance+immunotherapy+efficacy+in+solid+tumors%5BTitle%5D)+AND+%22J+Hematol+Oncol%22%5BJournal%5D)">Reshaping the systemic tumor immune environment (stie) and tumor immune microenvironment (time) to enhance immunotherapy efficacy in solid tumors.</a> J Hematol Oncol. 15 (1), 87(2022).</li>
<li>Mao, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Crosstalk+between+cancer-associated+fibroblasts+and+immune+cells+in+the+tumor+microenvironment%3A+New+findings+and+future+perspectives%5BTitle%5D)+AND+%22Mol+Cancer%22%5BJournal%5D)">Crosstalk between cancer-associated fibroblasts and immune cells in the tumor microenvironment: New findings and future perspectives.</a> Mol Cancer. 20 (1), 131(2021).</li>
<li>Roederer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spectral+compensation+for+flow+cytometry%3A+Visualization+artifacts%2C+limitations%2C+and+caveats%5BTitle%5D)+AND+%22Cytometry%22%5BJournal%5D)">Spectral compensation for flow cytometry: Visualization artifacts, limitations, and caveats.</a> Cytometry. 45 (3), 194-205 (2001).</li>
<li>Sahaf, B., Rahman, A., Maecker, H. T., Bendall, S. C. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSahaf%2C+B+%22Biomarkers+for+immunotherapy+of+cancer%3A+Methods+and+protocols%22">Biomarkers for immunotherapy of cancer: Methods and protocols</a>. , Springer. New York, New York, NY. (2020).</li>
<li>Turaç, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Combined+flow+cytometric+analysis+of+surface+and+intracellular+antigens+reveals+surface+molecule+markers+of+human+neuropoiesis%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis.</a> PLoS One. 8 (6), e68519(2013).</li>
<li>Wu, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Redefining+tumor-associated+macrophage+subpopulations+and+functions+in+the+tumor+microenvironment%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Redefining tumor-associated macrophage subpopulations and functions in the tumor microenvironment.</a> Front Immunol. 11, 1731(2020).</li>
<li>Elaldi, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+dimensional+imaging+mass+cytometry+panel+to+visualize+the+tumor+immune+microenvironment+contexture%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">High dimensional imaging mass cytometry panel to visualize the tumor immune microenvironment contexture.</a> Front Immunol. 12, 666233(2021).</li>
<li>Gadalla, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Validation+of+cytof+against+flow+cytometry+for+immunological+studies+and+monitoring+of+human+cancer+clinical+trials%5BTitle%5D)+AND+%22Front+Oncol%22%5BJournal%5D)">Validation of cytof against flow cytometry for immunological studies and monitoring of human cancer clinical trials.</a> Front Oncol. 9, 415(2019).</li>
<li>Ijsselsteijn, M. E., Van Der Breggen, R., Farina Sarasqueta, A., Koning, F., De Miranda, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+40-marker+panel+for+high+dimensional+characterization+of+cancer+immune+microenvironments+by+imaging+mass+cytometry%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">A 40-marker panel for high dimensional characterization of cancer immune microenvironments by imaging mass cytometry.</a> Front Immunol. 10, 2534(2019).</li>
<li>Zhang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry-based+peripheral+blood+analysis+as+a+novel+tool+for+early+detection+of+solid+tumours%3A+A+multicentre+study%5BTitle%5D)+AND+%22Gut%22%5BJournal%5D)">Mass cytometry-based peripheral blood analysis as a novel tool for early detection of solid tumours: A multicentre study.</a> Gut. 72 (5), 996-1006 (2023).</li>
<li>Zheng, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Trajectory+and+functional+analysis+of+pd-1%28high%29+cd4%28%2B%29cd8%28%2B%29+t+cells+in+hepatocellular+carcinoma+by+single-cell+cytometry+and+transcriptome+sequencing%5BTitle%5D)+AND+%22Adv+Sci%22%5BJournal%5D)">Trajectory and functional analysis of pd-1(high) cd4(+)cd8(+) t cells in hepatocellular carcinoma by single-cell cytometry and transcriptome sequencing.</a> Adv Sci. 7 (13), 2000224(2020).</li>
<li>Song, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protocol+for+isolating+single+cells+from+human+pancreatic+cancer+tissues+and+analyzing+major+immune+cell+populations+using+flow+cytometry%5BTitle%5D)+AND+%22STAR+Protoc%22%5BJournal%5D)">Protocol for isolating single cells from human pancreatic cancer tissues and analyzing major immune cell populations using flow cytometry.</a> STAR Protoc. 4 (4), 102679(2023).</li>
<li>Simoni, Y., Chng, M. H. Y., Li, S., Fehlings, M., Newell, E. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry%3A+A+powerful+tool+for+dissecting+the+immune+landscape%5BTitle%5D)+AND+%22Curr+Opin+Immunol%22%5BJournal%5D)">Mass cytometry: A powerful tool for dissecting the immune landscape.</a> Curr Opin Immunol. 51, 187-196 (2018).</li>
<li>Comi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coexpression+of+cd163+and+cd141+identifies+human+circulating+il-10-producing+dendritic+cells+%28dc-10%29%5BTitle%5D)+AND+%22Cell+Mol+Immunol%22%5BJournal%5D)">Coexpression of cd163 and cd141 identifies human circulating il-10-producing dendritic cells (dc-10).</a> Cell Mol Immunol. 17 (1), 95-107 (2020).</li>
<li>Fan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-dimensional+single-cell+analysis+delineates+peripheral+immune+signature+of+coronary+atherosclerosis+in+human+blood%5BTitle%5D)+AND+%22Theranostics%22%5BJournal%5D)">High-dimensional single-cell analysis delineates peripheral immune signature of coronary atherosclerosis in human blood.</a> Theranostics. 12 (15), 6809-6825 (2022).</li>
<li>Sheng, J., et al. Human endogenous retrovirus activation contributes to biliary atresia pathogenesis through re-education of resident macrophages. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSheng%2C+J+%22Biorxiv%22">Biorxiv</a>. , (2022).</li>
<li>Terekhova, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+atlas+of+healthy+human+blood+unveils+age-related+loss+of+nkg2c%28%2B%29gzmb%28-%29cd8%28%2B%29+memory+t+cells+and+accumulation+of+type+2+memory+t+cells%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Single-cell atlas of healthy human blood unveils age-related loss of nkg2c(+)gzmb(-)cd8(+) memory t cells and accumulation of type 2 memory t cells.</a> Immunity. 56 (12), 2836-2854.e9 (2023).</li>
<li>Sathaliyawala, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Distribution+and+compartmentalization+of+human+circulating+and+tissue-resident+memory+t+cell+subsets%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Distribution and compartmentalization of human circulating and tissue-resident memory t cell subsets.</a> Immunity. 38 (1), 187-197 (2013).</li>
<li>Lee, S., Vu, H. M., Lee, J. H., Lim, H., Kim, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advances+in+mass+spectrometry-based+single+cell+analysis%5BTitle%5D)+AND+%22Biology%22%5BJournal%5D)">Advances in mass spectrometry-based single cell analysis.</a> Biology. 12 (3), 395(2023).</li>
<li>Iyer, A., Hamers, A. A. J., Pillai, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cytof+for+the+masses%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Cytof for the masses.</a> Front Immunol. 13, 815828(2022).</li>
<li>Yuan, X., Wang, J., Huang, Y., Shangguan, D., Zhang, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+profiling+to+explore+immunological+heterogeneity+of+tumor+microenvironment+in+breast+cancer%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Single-cell profiling to explore immunological heterogeneity of tumor microenvironment in breast cancer.</a> Front Immunol. 12, 643692(2021).</li>
<li>Serban, G. M., Mănescu, I. B., Manu, D. R., Dobreanu, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimization+of+a+density+gradient+centrifugation+protocol+for+isolation+of+peripheral+blood+mononuclear+cells%5BTitle%5D)+AND+%22Acta+Marisiensis+-+Seria+Medica%22%5BJournal%5D)">Optimization of a density gradient centrifugation protocol for isolation of peripheral blood mononuclear cells.</a> Acta Marisiensis - Seria Medica. 64 (2), 83-90 (2018).</li>
<li>Shcherbakova, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Factors+affecting+cell+viability+during+the+enzymatic+dissociation+of+human+endocrine+tumor+tissues%5BTitle%5D)+AND+%22Biology+%28Basel%29%22%5BJournal%5D)">Factors affecting cell viability during the enzymatic dissociation of human endocrine tumor tissues.</a> Biology (Basel). 13 (9), 665(2024).</li>
<li>Devine, R. D., Alkhalaileh, H. S., Lyberger, J. M., Behbehani, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Alternative+methods+of+viability+determination+in+single+cell+mass+cytometry%5BTitle%5D)+AND+%22Cytometry+A%22%5BJournal%5D)">Alternative methods of viability determination in single cell mass cytometry.</a> Cytometry A. 99 (10), 1042-1053 (2021).</li>
<li>Gonzalez, V. D., Huang, Y. W., Fantl, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mass+cytometry+for+the+characterization+of+individual+cell+types+in+ovarian+solid+tumors%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Mass cytometry for the characterization of individual cell types in ovarian solid tumors.</a> Methods Mol Biol. 2424, 59-94 (2022).</li>
<li>Zabransky, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Profiling+of+syngeneic+mouse+hcc+tumor+models+as+a+framework+to+understand+anti-pd-1+sensitive+tumor+microenvironments%5BTitle%5D)+AND+%22Hepatology%22%5BJournal%5D)">Profiling of syngeneic mouse hcc tumor models as a framework to understand anti-pd-1 sensitive tumor microenvironments.</a> Hepatology. 77 (5), 1566-1579 (2023).</li>
<li>Levine, L. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+analysis+by+mass+cytometry+reveals+metabolic+states+of+early-activated+cd8%28%2B%29+t+cells+during+the+primary+immune+response%5BTitle%5D)+AND+%22Immunity%22%5BJournal%5D)">Single-cell analysis by mass cytometry reveals metabolic states of early-activated cd8(+) t cells during the primary immune response.</a> Immunity. 54 (4), 829-844.e5 (2021).</li>
<li>Lv, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunotherapy%3A+Reshape+the+tumor+immune+microenvironment%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Immunotherapy: Reshape the tumor immune microenvironment.</a> Front Immunol. 13, 844142(2022).</li>
<li>Zhou, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deciphering+the+tumor+immune+microenvironment+from+a+multidimensional+omics+perspective%3A+Insight+into+next-generation+car-t+cell+immunotherapy+and+beyond%5BTitle%5D)+AND+%22Mol+Cancer%22%5BJournal%5D)">Deciphering the tumor immune microenvironment from a multidimensional omics perspective: Insight into next-generation car-t cell immunotherapy and beyond.</a> Mol Cancer. 23 (1), 131(2024).</li>
<li>Hsieh, W. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spatial+multi-omics+analyses+of+the+tumor+immune+microenvironment%5BTitle%5D)+AND+%22J+Biomed+Sci%22%5BJournal%5D)">Spatial multi-omics analyses of the tumor immune microenvironment.</a> J Biomed Sci. 29 (1), 96(2022).</li>
<li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gdf15+induces+immunosuppression+via+cd48+on+regulatory+t+cells+in+hepatocellular+carcinoma%5BTitle%5D)+AND+%22J+Immunother+Cancer%22%5BJournal%5D)">Gdf15 induces immunosuppression via cd48 on regulatory t cells in hepatocellular carcinoma.</a> J Immunother Cancer. 9 (9), e002787(2021).</li>
<li>Krams, S. M., Schaffert, S., Lau, A. H., Martinez, O. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Applying+mass+cytometry+to+the+analysis+of+lymphoid+populations+in+transplantation%5BTitle%5D)+AND+%22Am+J+Transplant%22%5BJournal%5D)">Applying mass cytometry to the analysis of lymphoid populations in transplantation.</a> Am J Transplant. 17 (8), 1992-1999 (2017).</li>
</ol>

<table><tbody><tr><td>1×PBS</td><td>HyClone</td><td>SH30256.01</td><td></td></tr><tr><td>10×PBS</td><td>HyClone</td><td>SH30256.01</td><td></td></tr><tr><td>100 mm×20 mm tissue-culture-treated culture dish</td><td>Corning</td><td>430167</td><td></td></tr><tr><td>1000 mL pipette tips</td><td>Rainin</td><td>30389218</td><td></td></tr><tr><td>15 mL centrifuge tube</td><td>NEST</td><td>601052</td><td></td></tr><tr><td>200 mL pipette tips</td><td>Rainin</td><td>30389241</td><td></td></tr><tr><td>40 mm nylon cell strainer/70-mm nylon cell strainer</td><td>Falcon</td><td>352340</td><td></td></tr><tr><td>50 mL centrifuge tube</td><td>NEST</td><td>602052</td><td></td></tr><tr><td>70 μm syringe fifilter</td><td>Sangon Biotech</td><td>F613462-9001</td><td></td></tr><tr><td>Anti-Human CCR2 Antibody (clone: K036C2)</td><td>BioLegend</td><td>357224</td><td></td></tr><tr><td>Anti-Human CCR3 Antibody (clone: 5E8)</td><td>BioLegend</td><td>310724</td><td></td></tr><tr><td>Anti-Human CCR7 Antibody (clone: G043H7)</td><td>BioLegend</td><td>353240</td><td></td></tr><tr><td>Anti-Human CD103 Antibody (clone: Ber-ACT8)</td><td>BioLegend</td><td>350202</td><td></td></tr><tr><td>Anti-Human CD115 Antibody (clone: 9-4D2-1E4)</td><td>BioLegend</td><td>347314</td><td></td></tr><tr><td>Anti-Human CD117 Antibody (clone: 104D2)</td><td>BioLegend</td><td>313201</td><td></td></tr><tr><td>Anti-human CD11b Antibody (clone: 1CRF44)&nbsp;</td><td>BD</td><td>562721</td><td></td></tr><tr><td>Anti-human CD11c Antibody (clone: B-ly6)&nbsp;</td><td>BD</td><td>563026</td><td></td></tr><tr><td>Anti-Human CD123 Antibody (clone: 6H6)</td><td>BioLegend</td><td>306002</td><td></td></tr><tr><td>Anti-Human CD127 Antibody (clone: A019D5)</td><td>BioLegend</td><td>351337</td><td></td></tr><tr><td>Anti-Human CD13 Antibody (clone: WM19)</td><td>BioLegend</td><td>301701</td><td></td></tr><tr><td>Anti-Human CD138 Antibody (clone: MI15)</td><td>BioLegend</td><td>356535</td><td></td></tr><tr><td>Anti-human CD14 Antibody (clone: HCD14)</td><td>BioLegend</td><td>325604</td><td></td></tr><tr><td>Anti-Human CD141 Antibody (clone: M80)</td><td>BioLegend</td><td>344102</td><td></td></tr><tr><td>Anti-Human CD15 Antibody (clone: QA19A61)</td><td>BioLegend</td><td>376302</td><td></td></tr><tr><td>Anti-human CD16 Antibody (clone: B7311)&nbsp;</td><td>BD</td><td>561313</td><td></td></tr><tr><td>Anti-Human CD161 Antibody (clone: HP-3G10)</td><td>BioLegend</td><td>339902</td><td></td></tr><tr><td>Anti-Human CD163 Antibody (clone: GHI/61)</td><td>BioLegend</td><td>333603</td><td></td></tr><tr><td>Anti-Human CD169 Antibody (clone: 7-239)</td><td>BioLegend</td><td>346002</td><td></td></tr><tr><td>Anti-human CD19 Antibody (clone: HIB19)</td><td>BioLegend</td><td>302226</td><td></td></tr><tr><td>Anti-Human CD1c Antibody (clone: L161)</td><td>BioLegend</td><td>331501</td><td></td></tr><tr><td>Anti-Human CD20 Antibody (clone: 2H7)</td><td>BioLegend</td><td>302301</td><td></td></tr><tr><td>Anti-Human CD206 Antibody (clone: 15-2)</td><td>BioLegend</td><td>321151</td><td></td></tr><tr><td>Anti-Human CD24 Antibody (clone: ML5)</td><td>BioLegend</td><td>311129</td><td></td></tr><tr><td>Anti-Human CD25 Antibody (clone: BC96)</td><td>BioLegend</td><td>302624</td><td></td></tr><tr><td>Anti-human CD3 Antibody (clone: UCHT1)&nbsp;</td><td>BD</td><td>555916</td><td></td></tr><tr><td>Anti-Human CD31 Antibody (clone: W18200D)</td><td>BioLegend</td><td>375902</td><td></td></tr><tr><td>Anti-Human CD32 Antibody (clone: FUN-2)</td><td>BioLegend</td><td>303232</td><td></td></tr><tr><td>Anti-Human CD326 Antibody (clone: CO17-1A)</td><td>BioLegend</td><td>369812</td><td></td></tr><tr><td>Anti-Human CD33 Antibody (clone: WM53)</td><td>BioLegend</td><td>303402</td><td></td></tr><tr><td>Anti-human CD4 Antibody (clone: L200)&nbsp;</td><td>BD</td><td>563094</td><td></td></tr><tr><td>Anti-Human CD45 Antibody (clone: HI30)&nbsp;</td><td>BD</td><td>563716</td><td></td></tr><tr><td>Anti-Human CD45RO Antibody (clone: UCHL1)</td><td>BioLegend</td><td>304220</td><td></td></tr><tr><td>Anti-human CD56 Antibody (clone: 5.1H11)</td><td>BioLegend</td><td>362510</td><td></td></tr><tr><td>Anti-Human CD64 Antibody (clone: S18012C)</td><td>BioLegend</td><td>399502</td><td></td></tr><tr><td>Anti-Human CD66b Antibody (clone: 6/40c)</td><td>BioLegend</td><td>392917</td><td></td></tr><tr><td>Anti-human CD68 Antibody (clone: Y1/82A)</td><td>BioLegend</td><td>333808</td><td></td></tr><tr><td>Anti-Human CD69 Antibody (clone: FN50)</td><td>BioLegend</td><td>310902</td><td></td></tr><tr><td>Anti-Human CD7 Antibody (clone: 4H9/CD7)</td><td>BioLegend</td><td>395602</td><td></td></tr><tr><td>Anti-human CD8 Antibody (clone: RPA-T8)&nbsp;</td><td>BD</td><td>557750</td><td></td></tr><tr><td>Anti-Human CD80 Antibody (clone: W17149D)</td><td>BioLegend</td><td>375402</td><td></td></tr><tr><td>Anti-Human CD86 Antibody (clone: BU63)</td><td>BioLegend</td><td>374202</td><td></td></tr><tr><td>Anti-Human FOXP3 Antibody (clone: 206D)</td><td>BioLegend</td><td>320101</td><td></td></tr><tr><td>Anti-Human HLA_ABC Antibody (clone: W6/32)</td><td>BioLegend</td><td>311426</td><td></td></tr><tr><td>Anti-human HLA-DR Antibody (clone: L243)</td><td>BioLegend</td><td>307650</td><td></td></tr><tr><td>Anti-Human IgD Antibody (clone: IA6-2)</td><td>BioLegend</td><td>348211</td><td></td></tr><tr><td>Anti-Human Ki67 Antibody (clone: Ki-67)</td><td>BioLegend</td><td>350501</td><td></td></tr><tr><td>Anti-Human PD_L2 Antibody (clone: MH22B2)</td><td>BD</td><td>567783</td><td></td></tr><tr><td>Anti-Human PD1 Antibody (clone: EH12.2H7)</td><td>BioLegend</td><td>329951</td><td></td></tr><tr><td>Anti-Human PDL1 Antibody (clone: MIH2)</td><td>BioLegend</td><td>393602</td><td></td></tr><tr><td>Anti-human TCR-γδ Antibody (clone: B1)&nbsp;</td><td>BD</td><td>740415</td><td></td></tr><tr><td>Cell cryopreservation

massacytometrie-analyse van systemische en lokale immuunresponsen bij hepatocellulair carcinoom

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Massacytometrie-analyse van systemische en lokale immuunresponsen bij hepatocellulair carcinoom

Hepatocellular carcinoma (HCC) is one of the most common and deadliest forms of liver cancer worldwide. Despite advances in treatment, the prognosis for ...

mass-cytometry-analysis-systemic-local-immune-responses

Research

JoVE Journal

Biology

Biologie

Hier beschrijven we een methode om te scheiden en verrijken van de componenten van de tumor immuun en niet-immuun communicatie in gevestigde subcutane tumoren. Deze techniek maakt het mogelijk voor de afzonderlijke analyse van tumor immuun infiltreren en niet-immuun tumor breuken die uitgebreide karakterisering van de tumor immuun communicatie kunnen toestaan.

Verrijking en karakterisering van de Tumor immuun en niet-immune Microenvironments in gevestigde subcutane lymfkliertest tumoren

Hier beschrijven we een eencellige proteomic aanpak om te evalueren van immuun fenotypische en functionele (intracellulaire cytokine inductie) veranderingen in het perifere bloedmonsters geanalyseerd via massale cytometry.

Eencellige analyse van Immunophenotype en Cytokine productie in perifere bloed via massale Cytometry

Hier beschrijven we de ontwikkeling van een klinisch relevant muriene model van leverkanker die de typische immuunkenmerken van hepatocellulaire kanker (HCC) recapituleren.

Een oncogene hepatocyte-geïnduceerde Orthotopic muis model van hepatocellulaire kanker ontstaan in de setting van leverontsteking en fibrose

Ontduiking van natuurlijke killer (NK) celgemedieerde uitroeiing door kankercellen is belangrijk voor kanker initiatie en progressie. Hier presenteren we twee niet-radioactiviteit-gebaseerde protocollen om NK cel-gemedieerde cytotoxiciteit te evalueren in de richting van levertumorcellen. Daarnaast wordt een derde protocol gepresenteerd om NK-celmigratie te analyseren.

Meting van Natural Killer Cell-Gemedieerde Cytotoxiciteit en Migratie in de context van levertumorcellen

Hier beschrijven we een eenvoudige en toegankelijke strategie voor het visualiseren, kwantificeren en in kaart brengen van immuuncellen in formaline-vaste paraffine-ingebedde tumorweefselsecties. Deze methodologie combineert bestaande beeldvormings- en digitale analysetechnieken met als doel de multiplexingcapaciteit en multiparameteranalyse van beeldvormingstesten uit te breiden.

Visualisatie, kwantificering en mapping van immuuncelpopulaties in de tumormicro-omgeving

Uitgebreide in vitro modellen die de relevante menselijke ziekte getrouw samenvatten, ontbreken. De huidige studie presenteert driedimensionale (3D) tumor sferoïde creatie en cultuur, een betrouwbaar in vitro hulpmiddel om de tumor-stromale interactie in humaan hepatocellulair carcinoom te bestuderen.

Een driedimensionaal sferoïde model om de tumor-stromale interactie bij hepatocellulair carcinoom te onderzoeken

Dit protocol schetst de generatie van muizen van het menselijk immuunsysteem (HIS) voor immuno-oncologische studies. Instructies en overwegingen bij het gebruik van dit model voor het testen van menselijke immunotherapeutica op menselijke tumoren die in dit model zijn geïmplanteerd, worden gepresenteerd met de nadruk op het karakteriseren van de reactie van het menselijk immuunsysteem op de tumor.

Testen van kankerimmunotherapeutica in een gehumaniseerd muismodel met menselijke tumoren

Hier beschrijven we een eenvoudig, snel en betrouwbaar multiparametrisch flowcytometrie- en tumor-op-een-chip-ondersteund protocol om de stappen die de kankerimmuniteitscyclus vormen, te bewaken en te karakteriseren. Een grondig begrip van de wisselwerking tussen kanker en immuuncellen biedt inderdaad cruciale inzichten om tumoren te slim af te zijn en de klinische zorg te sturen.

Co-cultuur in vitro-systemen om de kanker-immuniteitscyclus te reproduceren

De aanwezigheid van mestcellen in de binnenmarge en peritumorgebieden van hepatocellulair carcinoom na resectie verleent een gunstige prognose. Deze studie onderschrijft QuPath-software voor beeldanalyse als een veelbelovend platform dat zou kunnen voldoen aan de behoefte aan reproduceerbaarheid, consistentie en nauwkeurigheid in digitale pathologie.

Mestcellen in de micro-omgeving van hepatocellulair carcinoom geven een gunstige prognose: een retrospectieve studie met behulp van QuPath-beeldanalysesoftware 

Deze video toont de dissectie en verwijdering van de foetale thymus en de voorbereiding van de ex vivo kweken van 2-dGuo behandeld thymus.

Bereiding van 2-dGuo behandelde Thymus Organ culturen

Leer massacytometrie-analyse van systemische en lokale immuunresponsen bij hepatocellulair carcinoom. Deze methode identificeert 14 celtypen op enkel-celniveau en onthult unieke expressieprofielen voor gedetailleerd immuunonderzoek.

Massacytometrie-analyse van systemische en lokale immuunresponsen bij hepatocellulair carcinoom

Meer video's verkennen