Name: Oprichting van rattenmodellen die genderbevestigende hormoontherapieën nabootsen (Video) | JoVE Journal
Uploaded: 2025-01-10T13:50:00.000+00:00
Duration: 6 min 24 s
Description: Leer hoe u rattenmodellen opzet die genderbevestigende hormoontherapieën nabootsen met testosteron of oestrogeen. Deze methode evalueert langetermijneffecten en interacties met milieuchemicaliën.

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</ol>

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oprichting van rattenmodellen die genderbevestigende hormoontherapieën nabootsen

Establishment of Rat Models Mimicking Gender-affirming Hormone Therapies

Oprichting van rattenmodellen die genderbevestigende hormoontherapieën nabootsen

Transgender (TG) people are individuals whose gender identity and sex assigned at birth do not match. They often undergo gender-affirming hormone therapy ...

establishment-rat-models-mimicking-gender-affirming-hormone

Research

JoVE Journal

Biology

Biologie

Ovariectomie en daaropvolgende vervanging van 17β-oestradiol door silastic capsules en perorale Nutella worden aangetoond bij ratten en muizen.

Ovariëctomie en 17β-estradiol Vervanging bij ratten en muizen: een visuele demonstratie

Gonadenhormonen zoals oestrogeen moduleren geheugenvorming in een aantal experimentele paradigma's, inclusief angstuitdovingsgeheugen. Dit protocol beschrijft een reeks methoden voor het onderzoeken van de invloed van gonadale hormonen specifiek tijdens uitdoving bij natuurlijk cyclische vrouwtjes, inclusief oestruscyclusbewaking en exogeen oestrogeentoediening.

Protocol voor het bestuderen van Uitsterven van geconditioneerde angst in Naturally Fietsen vrouwelijke ratten

Veel behandelingen en genetische mutaties van invloed op de timing van seksuele rijpheid en vruchtbaarheid. Dit protocol beschrijft een niet-invasieve methode om te evalueren van de pubertijd begin in muizen en ratten vóór het opzetten van een vruchtbaarheidsonderzoek in seksueel volwassen dieren.

Bepaling van reproductieve bevoegdheid door te bevestigen van pubertijd begin en uitvoeren van een test van de vruchtbaarheid in muizen en ratten

We beschrijven de ontwikkeling van een mager PCOS-achtige muismodel met dihydrotestosteron pellet te bestuderen van de pathofysiologie van PCOS en de nakomelingen van deze PCOS-achtige dammen.

Een muismodel van Hyperandrogenic te bestuderen polycysteus ovarium syndroom

Dit conflict model wordt gebruikt voor het meten van de bijzondere waardevermindering van remmende controle na blootstelling aan verslavende drugs, of andere factoren die invloed kunnen hebben op de remmende controle. Een seksuele stimulans en aversieve obstakel gelijktijdig worden gepresenteerd, dus mannelijke ratten te veroveren van de belemmering voor het benaderen van de seksuele beloning.

Een Conflict-Model van beloning-seeking gedrag in mannelijke ratten

Klinisch, oestrogeen deficiëntie bij menopauzale vrouwen kunnen verergeren de incidentie van lipide verstoring en atherosclerose. We vestigden een in vivo oestrogeen deficiëntie model door bilaterale ovariëctomie via een dubbele Dorsale-laterale incisie in apoE-/- muizen. Het muismodel is van toepassing voor het screenen van exogene oestrogeen behandelingen van cardiovasculaire disfunctie na de menopauze.

Een in vivo oestrogeen deficiëntie muis model voor het screenen van exogene oestrogeen behandelingen van cardiovasculaire disfunctie na de menopauze

Dit artikel is bedoeld om de methodologie voor lentivirale transgenese in rattenembryo's met behulp van meerdere injecties van een virussuspensie in de zygote perivitelline ruimte. Vrouwelijke ratten die zijn gepaard met een vruchtbare mannelijke stam met een andere dominante vacht kleur wordt gebruikt om pseudozwangere pleegmoeders te genereren.

Generatie transgene ratten met behulp van een Lentivirale Vector Approach

Veel knaagdiermodellen van endometriose worden beperkt door technische complexiteit, reproduceerbaarheid en/of behoefte aan immuungecompromitteerde dieren of speciale reportermuizen. We presenteren een vereenvoudigd systeem van laesie-inductie met behulp van elke experimentele muis met een onafhankelijk verifieerbaar, objectief scoresysteem en zonder vereiste voor ovariëctomie of overlevingsoperaties.

Een syngeneïsch murinemodel van endometriose met natuurlijk fietsende muizen

Deze studie beschrijft de cruciale factoren waarmee rekening moet worden gehouden bij experimentele ontwerpen met vrouwelijke ratten. In bredere zin dienen deze gegevens om stigma te verminderen en te helpen bij de ontwikkeling van meer inclusieve diagnostische en interventietools.

Knaagdier Estrous Cycle Monitoring met behulp van vaginale lavage: niet zoiets als een normale cyclus

Dit manuscript beschrijft een consistente manier om snel overleving uit te voeren knaagdierorchidtomieën en ovariëctomieën.

Muizen orchidectomie en ovariëctomie om de productie van geslachtshormonen te verminderen

Deze studie biedt een reproduceerbare en gestandaardiseerde methode om zwaarlijvige rattenmodellen vast te stellen die eenvoudige obesitas vertonen, vaak gekenmerkt door vette alvleesklier. Het bevat ook gedetailleerde protocollen voor bemonstering van pancreasweefsel en immunohistochemie.

Ontwikkeling en validatie van een methodologie voor het opstellen van zwaarlijvige rattenmodellen met typische vette alvleesklier

Leer hoe u rattenmodellen opzet die genderbevestigende hormoontherapieën nabootsen met testosteron of oestrogeen. Deze methode evalueert langetermijneffecten en interacties met milieuchemicaliën.

Oprichting van rattenmodellen die genderbevestigende hormoontherapieën nabootsen

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