Name: Detectie van gereguleerde moederkorenalkaloïden in voedselmatrices door middel van vloeistofchromatografie-ingesloten ionenmobiliteitsspectrometrie-time-of-flight massaspectrometrie (Video) | JoVE Journal
Uploaded: 2024-11-22T14:50:00.000+00:00
Duration: 8 min 56 s
Description: Leer een gevalideerde LC-IM-HRMS-methode voor detectie van moederkorenalkaloïden in voedselmatrices. Deze techniek verbetert voedselveiligheid door geavanceerde analytische identificatie van contaminanten.

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<meta charset="utf8"></head><body>Verbeterde isomere scheiding van moederkorenalkaloïden met behulp van LC-IMS-MS

<ol><li>Kanu, A. B., Dwivedi, P., Tam, M., Matz, L., Hill, H. H. Jr <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ion+mobility-mass+spectrometry%5BTitle%5D)+AND+%22J+Mass+Spectrom%22%5BJournal%5D)">Ion mobility-mass spectrometry.</a> J Mass Spectrom. 43 (1), 1-22 (2008).</li>
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<li>García-Juan, A., León, N., Armenta, S., Pardo, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+and+validation+of+an+analytical+method+for+the+simultaneous+determination+of+12+ergot%2C+2+tropane%2C+and+28+pyrrolizidine+alkaloids+in+cereal-based+food+by+LC-MS%2FMS%5BTitle%5D)+AND+%22Food+Res+Int%22%5BJournal%5D)">Development and validation of an analytical method for the simultaneous determination of 12 ergot, 2 tropane, and 28 pyrrolizidine alkaloids in cereal-based food by LC-MS/MS.</a> Food Res Int. 174 (Pt 1), 113614(2023).</li>
<li>Carbonell-Rozas, L., Mahdjoubi, C. K., Arroyo-Manzanares, N., García-Campaña, A. M., Gámiz-Gracia, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Occurrence+of+ergot+alkaloids+in+barley+and+wheat+from+Algeria%5BTitle%5D)+AND+%22Toxins%22%5BJournal%5D)">Occurrence of ergot alkaloids in barley and wheat from Algeria.</a> Toxins. 13 (5), 316(2021).</li>
<li>Carbonell-Rozas, L., Gámiz-Gracia, L., Lara, F. J., García-Campaña, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Determination+of+the+main+ergot+alkaloids+and+their+epimers+in+oat-based+functional+foods+by+ultra-high+performance+liquid+chromatography+tandem+mass+spectrometry%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Determination of the main ergot alkaloids and their epimers in oat-based functional foods by ultra-high performance liquid chromatography tandem mass spectrometry.</a> Molecules. 26 (12), 3717(2021).</li>
<li>Carbonell-Rozas, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ion+mobility-mass+spectrometry+to+extend+analytical+performance+in+the+determination+of+ergot+alkaloids+in+cereal+samples%5BTitle%5D)+AND+%22J+Chromatogr+A%22%5BJournal%5D)">Ion mobility-mass spectrometry to extend analytical performance in the determination of ergot alkaloids in cereal samples.</a> J Chromatogr A. 1682, 463502(2022).</li>
<li>European Commission. Commission Implementing Regulation (EU) 2023/2782 of 14 December 2023 laying down the methods of sampling and analysis for the control of the levels of mycotoxins in food and repealing Regulation (EC) No 401/2006. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Off+J+Eur+Union%22">Off J Eur Union</a>. , (2023).</li>
<li>Carbonell-Rozas, L., Vander Cruyssen, L., Dall'Asta, C., Leggieri, M. C., Battilani, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fit-for-purpose+method+development+to+determine+co-occurring+multiclass+mycotoxins+in+apple+and+apple+puree+samples%5BTitle%5D)+AND+%22Food+Anal+Methods%22%5BJournal%5D)">Fit-for-purpose method development to determine co-occurring multiclass mycotoxins in apple and apple puree samples.</a> Food Anal Methods. 16 (8), 1403-1412 (2023).</li>
<li>Laouni, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Emerging+mycotoxin+occurrence+in+chicken+feed+and+eggs+from+Algeria%5BTitle%5D)+AND+%22Mycotoxin+Res%22%5BJournal%5D)">Emerging mycotoxin occurrence in chicken feed and eggs from Algeria.</a> Mycotoxin Res. 40, 447-456 (2024).</li>
<li>Ben Hassouna, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mycotoxin+occurrence+in+milk+and+durum+wheat+samples+from+Tunisia+using+dispersive+liquid-liquid+microextraction+and+liquid+chromatography+with+fluorescence+detection%5BTitle%5D)+AND+%22Toxins%22%5BJournal%5D)">Mycotoxin occurrence in milk and durum wheat samples from Tunisia using dispersive liquid-liquid microextraction and liquid chromatography with fluorescence detection.</a> Toxins. 15 (11), 633(2023).</li>
<li>Narváez, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Occurrence+and+exposure+assessment+of+mycotoxins+in+ready-to-eat+tree+nut+products+through+ultra-high+performance+liquid+chromatography+coupled+with+high+resolution+Q-orbitrap+mass+spectrometry%5BTitle%5D)+AND+%22Metabolites%22%5BJournal%5D)">Occurrence and exposure assessment of mycotoxins in ready-to-eat tree nut products through ultra-high performance liquid chromatography coupled with high resolution Q-orbitrap mass spectrometry.</a> Metabolites. 10 (9), 344(2020).</li>
<li>Arroyo-Manzanares, N., Rodríguez-Estévez, V., García-Campaña, A. M., Castellón-Rendón, E., Gámiz-Gracia, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Determination+of+principal+ergot+alkaloids+in+swine+feeding%5BTitle%5D)+AND+%22J+Sci+Food+Agric%22%5BJournal%5D)">Determination of principal ergot alkaloids in swine feeding.</a> J Sci Food Agric. 101 (12), 5214-5224 (2021).</li>
<li>Pereira, V. L., Fernandes, J. O., Cunha, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparative+assessment+of+three+cleanup+procedures+after+QuEChERS+extraction+for+determination+of+trichothecenes+%28type+A+and+type+B%29+in+processed+cereal-based+baby+foods+by+GC-MS%5BTitle%5D)+AND+%22Food+Chem%22%5BJournal%5D)">Comparative assessment of three cleanup procedures after QuEChERS extraction for determination of trichothecenes (type A and type B) in processed cereal-based baby foods by GC-MS.</a> Food Chem. 182, 143-149 (2015).</li>
<li>Tuzimski, T., Szubartowski, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Method+development+for+selected+bisphenols+analysis+in+sweetened+condensed+milk+from+a+can+and+breast+milk+samples+by+HPLC-DAD+and+HPLC-QqQ-MS%3A+Comparison+of+sorbents+%28Z-SEP%2C+Z-SEP+Plus%2C+PSA%2C+C18%2C+chitin+and+EMR-lipid%29+for+clean-up+of+QuEChERS+extract%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Method development for selected bisphenols analysis in sweetened condensed milk from a can and breast milk samples by HPLC-DAD and HPLC-QqQ-MS: Comparison of sorbents (Z-SEP, Z-SEP Plus, PSA, C18, chitin and EMR-lipid) for clean-up of QuEChERS extract.</a> Molecules. 24 (11), 2093(2019).</li>
<li>Łozowicka, B., Rutkowska, E., Jankowska, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+QuEChERS+modifications+on+recovery+and+matrix+effect+during+the+multi-residue+pesticide+analysis+in+soil+by+GC%2FMS%2FMS+and+GC%2FECD%2FNPD%5BTitle%5D)+AND+%22Environ+Sci+Pollut+Res%22%5BJournal%5D)">Influence of QuEChERS modifications on recovery and matrix effect during the multi-residue pesticide analysis in soil by GC/MS/MS and GC/ECD/NPD.</a> Environ Sci Pollut Res. 24 (8), 7124-7138 (2017).</li>
<li>Schummer, C., Xandonella, I., van Nieuwenhuyse, A., Moris, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Epimerization+of+ergot+alkaloids+in+feed%5BTitle%5D)+AND+%22Heliyon%22%5BJournal%5D)">Epimerization of ergot alkaloids in feed.</a> Heliyon. 6 (6), e04336(2020).</li>
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</ol>

<table><tbody><tr><td>Acetonitrile</td><td>VWR</td><td>83640.32</td><td></td></tr><tr><td>Bernstein glasbuizen 4 mL</td><td>VWR</td><td>548-0052</td><td></td></tr><tr><td>Bernstein glasbuizen 12 mL</td><td>VWR</td><td>548-0903</td><td></td></tr><tr><td>Bernstein vials 1.5 mL</td><td>Agilent</td><td>5190-9063</td><td></td></tr><tr><td>Ammonium carbonaat</td><td>Fluka</td><td>9716</td><td></td></tr><tr><td>Analytische weegschaal BAS 31&nbsp;</td><td>Boeco</td><td>4400519</td><td></td></tr><tr><td>Weegschaal CP 323 S&nbsp;</td><td>Sartorius</td><td>23-84182</td><td></td></tr><tr><td>C18</td><td>Supelco</td><td>52604-U</td><td></td></tr><tr><td>Centrifugebuizen, 15 mL</td><td>VWR</td><td>525-1082</td><td></td></tr><tr><td>Centrifugebuizen, 50 mL</td><td>VWR</td><td>525-0155</td><td></td></tr><tr><td>Centrifuge Universal 320 R</td><td>Hettich</td><td>1406</td><td></td></tr><tr><td>Compass HyStar&nbsp;</td><td>Bruker</td><td></td><td>Acquisitiesoftware</td></tr><tr><td>DataAnalysis</td><td>Bruker</td><td></td><td>Kwalitatieve software</td></tr><tr><td>Elute PLUS&nbsp;UHPLC</td><td>Bruker</td><td></td><td></td></tr><tr><td>EVA EC-S verdamper</td><td>VLM</td><td>V830.012.12</td><td></td></tr><tr><td>Mierenzuur GR voor analyse ACS, Reag. Ph Eur</td><td>Merck</td><td>100264</td><td></td></tr><tr><td>Grinder TitanMill300</td><td>Cecotec</td><td>1559</td><td></td></tr><tr><td>Methanol</td><td>VWR</td><td>83638.32</td><td></td></tr><tr><td>Milli-Q waterzuiveringssysteem (18.2 M&Omega; cm)</td><td>Millipore</td><td>ZD5211584</td><td></td></tr><tr><td>Pipetpunten 1- 5 mL</td><td>Labortecnic</td><td>162005</td><td></td></tr><tr><td>Pipetpunten 100 - 1000 &micro;L</td><td>Labortecnic</td><td>1622222</td><td></td></tr><tr><td>Pipetpunten 5 - 200 &micro;L</td><td>Labortecnic</td><td>162001</td><td></td></tr><tr><td>Pipet Transferpette S variabel, DE-M 10 - 100 &micro;L</td><td>BRAND</td><td>704774</td><td></td></tr><tr><td>Pipet Transferpette S variabel, DE-M 100 - 1000 &micro;L</td><td>BRAND</td><td>704780</td><td></td></tr><tr><td>Pipet Transferpette S variabel, DE-M 500 - 5000 &micro;L</td><td>BRAND</td><td>704782</td><td></td></tr><tr><td>Spuit 2 mL</td><td>VWR</td><td>613-2003</td><td></td></tr><tr><td>Spuitfilter 13 mm, 0.22&micro;m</td><td>Phenomenex</td><td>AF-8-7707-12</td><td></td></tr><tr><td>TASQ</td><td>Bruker</td><td></td><td>Kwantitatieve software</td></tr><tr><td>timsTOFPro2 IM-HRMS</td><td>Bruker</td><td></td><td></td></tr><tr><td>Vortex Genie 2</td><td>Scientific Industries</td><td>15547335</td><td></td></tr><tr><td>Zorbax Eclipse Plus RRHD C18 kolom (50&nbsp;x&nbsp;2.1 mm, 1.8 &micro;m deeltjesgrootte)</td><td>Agilent&nbsp;</td><td>959757-902</td><td></td></tr><tr><td>Z-Sep+</td><td>Supelco</td><td>55299-U</td><td>Zirconium-gebaseerd sorptiemiddel</td></tr><tr><td>Ergot alkaloïden</td><td></td><td></td><td>CAS-register sorptiemiddel</td></tr><tr><td>Ergocornine (Eco)</td><td>Techno Spec</td><td>E178</td><td>564-36-3</td></tr><tr><td>Ergocorninine (Econ)</td><td>Techno Spec</td><td>E130</td><td>564-37-4</td></tr><tr><td>Ergocristine (Ecr)</td><td>Techno Spec</td><td>E180</td><td>511-08-0</td></tr><tr><td>Ergocristinine (Ecrn)</td><td>Techno Spec</td><td>E188</td><td>511-07-9</td></tr><tr><td>Ergokryptine (Ekr)</td><td>Techno Spec</td><td>E198</td><td>511-09-1</td></tr><tr><td>Ergopkryptinine (Ekrn)</td><td>Techno Spec</td><td>E190</td><td>511-10-4</td></tr><tr><td>Ergometrine (Em)</td><td>Romer Labs</td><td>"002067"</td><td>60-79-7</td></tr><tr><td>Ergometrinine (Emn)</td><td>Romer Labs</td><td>LMY-090-5ML</td><td>479-00-5</td></tr><tr><td>Ergosine (Es)</td><td>Techno Spec</td><td>E184</td><td>561-94-4</td></tr><tr><td>Ergosinine (Esn)</td><td>Techno Spec</td><td>E194</td><td>596-88-3</td></tr><tr><td>Ergotamine (Et)</td><td>Romer Labs</td><td>"002069"</td><td>113-15-5</td></tr><tr><td>Ergotaminine (Etn)</td><td>Romer Labs</td><td>"002075"</td><td>639-81-6</td></tr></tbody></table>

detectie van gereguleerde moederkorenalkaloïden in voedselmatrices door middel van vloeistofchromatografie-ingesloten ionenmobiliteitsspectrometrie-time-of-flight massaspectrometrie

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Detectie van gereguleerde moederkorenalkaloïden in voedselmatrices door middel van vloeistofchromatografie-ingesloten ionenmobiliteitsspectrometrie-time-of-flight massaspectrometrie

Ion mobility mass spectrometry (IMS) acts as an additional separation dimension when integrated into liquid chromatography-mass spectrometry (LC-MS) ...

detection-regulated-ergot-alkaloids-food-matrices-liquid

Research

JoVE Journal

Chemistry

Scheikunde

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Deze studie ontwikkelde een hoogpresterende vloeistofchromatografie in combinatie met triple quadrupole mass spectrometry (HPLC-QQQ-MS) methode om tropaanalkaloïden (hyoscyamine, anisomine, scopolamine) in Anisodus tanguticus te identificeren en te kwantificeren. De methode bleek snel, nauwkeurig en betrouwbaar voor high-throughput analyse van medicinale planten.

Een efficiënte en snelle HPLC-QQQ-MS-methode voor de kwantificatie van tropaanalkaloïden in medicinale planten

Non-gerichte metabolietprofilering door ultra-prestatie vloeistofchromatografie gekoppeld aan massaspectrometrie (UPLC-MS) is een krachtige techniek om metabolisme te onderzoeken. Dit artikel schetst een typische werkstroom die wordt gebruikt voor non-gerichte metabolietprofilering van serum, inclusief monsterorganisatie en -voorbereiding, dataverwerving, data-analyse, kwaliteitscontrole en metabolietidentificatie.

Large Scale Niet-gerichte metaboloom profilering van Serum door Ultra Performance Liquid Chromatography-massaspectrometrie (UPLC-MS)

Ongerichte metabolomics biedt een hypothese genererende momentopname van een metabool profiel. Dit protocol zal de extractie en analyse van metabolieten uit cellen, serum of weefsel tonen. Een reeks van metabolieten worden onderzocht met behulp van vloeistof-vloeistof fase-extractie, microflow UltraPerformance vloeistofchromatografie / hoge-resolutie massaspectrometrie (UPLC-HRMS) gekoppeld aan differentiële analyse software.

Ongerichte Metabolomics uit biologische grondstoffen gebruiken UltraPerformance Liquid Chromatography-hoge resolutie massaspectrometrie (UPLC-HRMS)

Een protocol voor het bepalen van de effectiviteit van photocatalysts in de binnenlucht concentratie (ppb) model vluchtige organische koolstofatomen vernederende zoals 2-propanol wordt beschreven.

Analyseren van de foto-oxidatie van 2-propanol bij binnenlucht niveau concentraties met behulp van spectrometrie van de mobiliteit van veld asymmetrische Ion

Hier beschrijven we een chromatografische test in combinatie met de ion mobiliteit scheiding van peptide precursoren gevolgd door de hoge resolutie (~ 30.000) MS-detectie van peptide fragmenten voor de kwantificering van puntige peptide normen in een monoclonal antilichaam Digest.

Een HS-MRM Assay voor de kwantificering van de gastheercel eiwitten in Protein biofarmaceutica door vloeistofchromatografie Ion mobiliteit QTOF massaspectrometrie

Time-of-Flight secundaire ionenmassa spectrometrie wordt toegepast om de chemische mapping en corrosie morfologie aan te tonen op de metaal-verf-interface van een aluminium legering na blootstelling aan een zoutoplossing in vergelijking met een specimen blootgesteld aan lucht.

Imaging corrosie bij de metaal verf-interface met behulp van tijd-of-Flight secundaire Ionenmassa spectrometrie

Leer een gevalideerde LC-IM-HRMS-methode voor detectie van moederkorenalkaloïden in voedselmatrices. Deze techniek verbetert voedselveiligheid door geavanceerde analytische identificatie van contaminanten.

Detectie van gereguleerde moederkorenalkaloïden in voedselmatrices door middel van vloeistofchromatografie-ingesloten ionenmobiliteitsspectrometrie-time-of-flight massaspectrometrie (Video) | JoVE Journal

Detectie van gereguleerde moederkorenalkaloïden in voedselmatrices door middel van vloeistofchromatografie-ingesloten ionenmobiliteitsspectrometrie-time-of-flight massaspectrometrie

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