Name: Het vastleggen van veelvoorkomende fragiele site-onderbrekingen door native γH2A. X Kanaal (Video) | JoVE Journal
Uploaded: 2025-01-24T14:20:00.000+00:00
Duration: 9 min 46 s
Description: Ontdek een snelle methode voor het detecteren van fragiele site-onderbrekingen met native γH2A.X ChIP in kankercellen. Deze aanpak vermindert arbeid en tijd zonder betrouwbaarheid te verliezen.

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</ol>

<table><tbody><tr><td>0.2 &micro;m nitrocellulose membrane</td><td>Amersham</td><td>10600011</td><td></td></tr><tr><td>Actin B</td><td>proteintech</td><td>20536-1-AP</td><td></td></tr><tr><td>Aphidicolin</td><td>MedChemExpress</td><td>HY-N6733</td><td></td></tr><tr><td>ChIP-grade magnetic Protein A/G beads</td><td>ThermoFisher</td><td>26162</td><td></td></tr><tr><td>Clarity Western ECL Substrate</td><td>Bio-Rad</td><td>#1705061</td><td></td></tr><tr><td>Glycogen, molecular biology grade</td><td>ThermoFisher</td><td>Cat. No.&nbsp; R0561</td><td></td></tr><tr><td>HRP-conjugated secondary antibody&nbsp;</td><td>proteintech</td><td>SA00001-2</td><td></td></tr><tr><td>hydroxyurea&nbsp;</td><td>MedChemExpress</td><td>HY-B0313</td><td></td></tr><tr><td>Micrococcal Nuclease&nbsp;</td><td>NEB</td><td>M0247S</td><td></td></tr><tr><td>normal IgG&nbsp;</td><td>Santa Cruz</td><td>sc-2025</td><td></td></tr><tr><td>Taq Universal SYBR Green Supermix&nbsp;</td><td>BioRad</td><td>1725120</td><td></td></tr><tr><td>&gamma;H2A.X antibody&nbsp; (for ChIP)</td><td>Sigma-Aldrich</td><td>05-636</td><td></td></tr><tr><td>&gamma;H2A.X antibody (for WB)</td><td>Cell Signaling</td><td>#25955</td><td></td></tr></tbody></table>

het vastleggen van veelvoorkomende fragiele site-onderbrekingen door native γh2a. x kanaal

Capturing Common Fragile Site Breaks by Native &#947;H2A.X ChIP

Het vastleggen van veelvoorkomende fragiele site-onderbrekingen door native γH2A. X Kanaal

Replication stress induced by exposure to extrinsic agents can lead to DNA breaks at common fragile sites, which are regions in the genome known to be ...

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Hier presenteren we een protocol dat beschrijft de fabricage van rekbare, dual channel, orgel chip microfluidic cel cultuur apparaten voor Recapitulerend orgel-niveau functionaliteit in vitro.

Schaalbare fabricage van rekbare, Dual Channel, Microfluidic orgel Chips

Dit document beschrijft een protocol samen met een vergelijkende studie van twee microfluïde fabricagetechnieken, namelijk fotolithografie/wet-etching/thermisch-bonding en Selective Laser-induced Etching (SLE), die geschikt zijn voor hogedrukomstandigheden. Deze technieken vormen het mogelijk maken van platforms voor directe observatie van de vloeistofstroom in surrogaatdoorlatende media en gebroken systemen onder reservoiromstandigheden.

Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO 2 Foam Transport in Fractured Unconventional Reservoirs Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO 2 Foam Transport in Fractured Unconventional Reservoirs Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs Microflu

Hier presenteren we een protocol voor het uitvoeren van TEM-gasreactie-experimenten met gesloten cellen in situ, terwijl we verschillende veelgebruikte monstervoorbereidingsmethoden beschrijven.

In situ gesloten-cel gasreacties uitvoeren in de transmissie-elektronenmicroscoop

Een protocol voor het kweken en manipuleren van embryonaal weefsel van muizen op een microfluïdische chip wordt verstrekt. Door pulsen van pathway modulators toe te passen, kan dit systeem worden gebruikt om signaaloscillaties extern te regelen voor het functionele onderzoek van muis somitogenese.

Een microfluïdische benadering voor het functioneel onderzoek van signaaloscillaties die somitogenese beheersen

Dit artikel volgt de levenscyclus van een siliciumnitridemembraanresonator, van ontwerp via fabricage tot karakterisering.

Fabricage en karakterisering van high-Q siliciumnitride-membraanresonatoren

Een nieuw afdichtingsprotocol om waterdichte, siliconengebaseerde afdichtingen te produceren voor membranen van polydimethylsiloxaan (PDMS) chipapparaten. Bedoeld voor laboratoria die chipmodellen opzetten en kleinschalige chipplatformontwikkeling. We demonstreren het protocol met behulp van plastic en native weefselmembranen. Deze procedure vereist alleen PDMS, tolueen, schimmel, membraan en een vacuümkamer.

Toegankelijke siliconenchip-tot-membraan-afdichtingsprocedure voor flexibele, betrouwbare hechting

Ontdek een snelle methode voor het detecteren van fragiele site-onderbrekingen met native γH2A.X ChIP in kankercellen. Deze aanpak vermindert arbeid en tijd zonder betrouwbaarheid te verliezen.

Het vastleggen van veelvoorkomende fragiele site-onderbrekingen door native γH2A. X Kanaal

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