Name: Chirurgische implantaatprocedure en bedradingsconfiguratie voor continue langdurige EEG/ECG-monitoring bij konijnen (Video) | JoVE Journal
Uploaded: 2025-01-24T14:45:00.000+00:00
Duration: 8 min 36 s
Description: Leer een chirurgische implantaatprocedure voor continue EEG/ECG-monitoring bij konijnen. Dit systeem maakt gelijktijdige registratie van hersen- en hartactiviteit mogelijk zonder direct toezicht, wat essentieel is voor onderzoek naar hartritmestoornissen en epileptische aanvallen.

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<meta charset="utf8"></head><body>Continue EEG/ECG-opnames bij konijnen met behulp van onderhuidse plaatsing van elektroden

<ol><li>Bosinski, C., Wagner, K., Zhou, X., Liu, L., Auerbach, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multi-system+monitoring+for+identification+of+seizures%2C+arrhythmias+and+apnea+in+conscious+restrained+rabbits%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Multi-system monitoring for identification of seizures, arrhythmias and apnea in conscious restrained rabbits.</a> J Vis Exp. (169), e62256(2021).</li>
<li>Finlay, M. C., Lambiase, P. D., Ben-Simon, R., Taggart, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+mental+stress+on+dynamic+electrophysiological+properties+of+the+endocardium+and+epicardium+in+humans%5BTitle%5D)+AND+%22Heart+Rhythm%22%5BJournal%5D)">Effect of mental stress on dynamic electrophysiological properties of the endocardium and epicardium in humans.</a> Heart Rhythm. 13 (1), 175-182 (2016).</li>
<li>Shen, M. J., Zipes, D. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Role+of+the+autonomic+nervous+system+in+modulating+cardiac+arrhythmias%5BTitle%5D)+AND+%22Circulation+Res%22%5BJournal%5D)">Role of the autonomic nervous system in modulating cardiac arrhythmias.</a> Circulation Res. 114 (6), 1004-1021 (2014).</li>
<li>Xu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Juvenile+myoclonic+epilepsy+and+sleep%5BTitle%5D)+AND+%22Epilepsy+Behav%22%5BJournal%5D)">Juvenile myoclonic epilepsy and sleep.</a> Epilepsy Behav. 80, 326-330 (2018).</li>
<li>Bazil, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Seizure+modulation+by+sleep+and+sleep+state%5BTitle%5D)+AND+%22Brain+Res%22%5BJournal%5D)">Seizure modulation by sleep and sleep state.</a> Brain Res. 1703, 13-17 (2019).</li>
<li>Ryan, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Heart+rate+and+autonomic+biomarkers+distinguish+convulsive+epileptic+vs.+Functional+or+dissociative+seizures%5BTitle%5D)+AND+%22Seizure%22%5BJournal%5D)">Heart rate and autonomic biomarkers distinguish convulsive epileptic vs. Functional or dissociative seizures.</a> Seizure. 111, 178-186 (2023).</li>
<li>Brunner, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanisms+of+cardiac+arrhythmias+and+sudden+death+in+transgenic+rabbits+with+long+QT+syndrome%5BTitle%5D)+AND+%22J+Clin+Invest%22%5BJournal%5D)">Mechanisms of cardiac arrhythmias and sudden death in transgenic rabbits with long QT syndrome.</a> J Clin Invest. 118, 2246-2259 (2008).</li>
<li>Edwards, A. G., Louch, W. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Species-dependent+mechanisms+of+cardiac+arrhythmia%3A+A+cellular+focus%5BTitle%5D)+AND+%22Clin+Med+Insights+Cardiol%22%5BJournal%5D)">Species-dependent mechanisms of cardiac arrhythmia: A cellular focus.</a> Clin Med Insights Cardiol. 11, 1179546816686061(2017).</li>
<li>Kaha Sciences. , Ad instruments. 
 <a target="_blank" href="http://https://www.adinstruments.com/kaha?creative=524197312959&keyword=kaha%20telemetry&matchtype=e&network=g&device=c&gad_source=1&gclid=CjwKCAjw8fu1BhBsEiwAwDrsjJLJeUjMCwMe-kCxFg2QdpP94gaAOd8qS6UBE6iU-IdP2XGxMvbT7hoCDZAQAvD_BwE">http://https://www.adinstruments.com/kaha?creative=524197312959&keyword=kaha%20telemetry&matchtype=e&network=g&device=c&gad_source=1&gclid=CjwKCAjw8fu1BhBsEiwAwDrsjJLJeUjMCwMe-kCxFg2QdpP94gaAOd8qS6UBE6iU-IdP2XGxMvbT7hoCDZAQAvD_BwE</a> (2024).</li>
<li>Stellar telemetry. , TSE Systems. 
 <a target="_blank" href="https://www.tse-systems.com/products/stellar-implantable-telemetry/">https://www.tse-systems.com/products/stellar-implantable-telemetry/</a> (2024).</li>
<li>Dsi implantable telemetry. , DSI. 
 <a target="_blank" href="https://www.datasci.com/products/implantable-telemetry">https://www.datasci.com/products/implantable-telemetry</a> (2024).</li>
<li>Stellar telemetry. , Kruuse televet ii, telemetric ecg & holter. 
 <a target="_blank" href="https://kruuse.com/products/consultation/examination/ekg/kruuse-televet-ii-telemetric-ecg-holter">https://kruuse.com/products/consultation/examination/ekg/kruuse-televet-ii-telemetric-ecg-holter</a> (2024).</li>
<li>Mishra, V., Gautier, N. M., Glasscock, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+Video-EEG-ECG+monitoring+to+identify+neurocardiac+dysfunction+in+mouse+models+of+epilepsy%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Simultaneous Video-EEG-ECG monitoring to identify neurocardiac dysfunction in mouse models of epilepsy.</a> J Vis Exp. (131), e57300(2018).</li>
<li>eeg/emg systems. , Pinnacle technology. 
 <a target="_blank" href="https://www.pinnaclet.com/eeg-emg-systems.html">https://www.pinnaclet.com/eeg-emg-systems.html</a> (2024).</li>
</ol>

<table><tbody><tr><td>3:1 heat shrink wrap with adhesive</td><td>N/A</td><td>Wirefly</td><td>Adhesive lined polyolefin tubing designed to shrink 1/3 diameter when heated. Isolate electrodes when connecting wires and plugs, connecting pieces of the wire harness together</td></tr><tr><td>Absorbent Pads</td><td>-</td><td>any</td><td>Absorbent pads for Fluid collection</td></tr><tr><td>BD E-Z Scrub 116 Surgical Scrub Brush/Sponge</td><td>371163</td><td>BD E-Z Scrub</td><td>3% PCMX Surgical Scrub Brush/Sponge To scrub prior to operating for aseptic technique</td></tr><tr><td>Biopsy Punches</td><td>1315</td><td>ARGENT, McKesson</td><td>6 mm circular cutting blade for circular incision for port</td></tr><tr><td>Buprenorphine</td><td>-</td><td>-</td><td>0.3 mg/mL Buprenorphine For post-operative pain control</td></tr><tr><td>Butt connectors</td><td>X003C7S38P</td><td>SVAAR</td><td>Tinned copper butt splice 26-22 AWG non-insulated butt connectors Connecting carbon fiber wires from the electrodes to the plug</td></tr><tr><td>Cautery Pen</td><td>https://jorvet.com/product/cautery-kit-accessories/</td><td>Jorgensen Laboratories, Inc.</td><td>Hand-held Bovie Cautery Pen For controlling bleeding</td></tr><tr><td>Coaxial cable through-wall bushing</td><td>COAX BUSH WH</td><td>Commercial Electric</td><td>Plastic bushing with 6.9mm inner diameter opening and 1cm+ post Wire port</td></tr><tr><td>Cohesiant Wrap</td><td>https://healthypets.com/vetone-cohesiant-wrap-blue-2x5yd/</td><td>AmerisourceBergen</td><td>4in x 5yd self-adherent wrap For post-operative wound protection</td></tr><tr><td>Disposable Scalpel with Handle</td><td>https://www.securos.com/</td><td>Securos Surgical AmerisourceBergen</td><td>Sterile disposable scalpels For making incisions</td></tr><tr><td>Electrical Slip Ring</td><td></td><td>Taidacent</td><td>High Speed Ball Conductive Slip Ring 12 way 2A Collector ring rotary joint rotary connector, Electrical slip ring to allow free movement of animal without binding up the wire harness</td></tr><tr><td>Eye lube pro</td><td>https://domespharma.us/eye-lube-pro-detailer-final-v6/</td><td>Sentrx</td><td>Lubricating Gel Prevents eye drying/damage during anesthesia</td></tr><tr><td>GLUture</td><td>503763</td><td>World Precision Instrument</td><td>Topical Tissue Adhesive For closure of incision sites&nbsp;</td></tr><tr><td>Isoflurane</td><td>ANADA # 200-129</td><td>Isospire</td><td>Isoflurane For anesthesia</td></tr><tr><td>Isopropyl alcohol</td><td>500205</td><td>VETone</td><td>70% isopropyl alcohol Topical antiseptic</td></tr><tr><td>Jet Liquid</td><td>1403</td><td>Lang Dental Mfg. Co., Inc.</td><td>Fast curing acrylic resin liquid Dental acrylic resin liquid for sealing the bushing and the wires for the wire exit port.&nbsp; Also used to seal the port to the skin.</td></tr><tr><td>Jet Tooth Shade Powder</td><td>1420</td><td>Lang Dental Mfg. Co., Inc.</td><td>Fast curing provisional crown and bridge acrylic resin powder. Dental acrylic powder for sealing the bushing and the wires for the wire exit port.&nbsp; Also used to seal the port to the skin.</td></tr><tr><td>Ketamine</td><td>-</td><td>-</td><td>100 mg/mL Ketamine For sedation</td></tr><tr><td>Metal shower sheathing</td><td>HOS-960M</td><td>Waterpik</td><td>Ultra-Flexible Replacement Metal Shower Hose, Extra Long for Handheld Shower Heads, 96-inch, Chrome Metal sheathing for wire harness</td></tr><tr><td>MR Conditional/CT Electrodes Array and Extension Set</td><td>MRQW32415</td><td>RhythmLink</td><td>Quick connect system with male-female plugs and 8 leads. Plugs used for port-wire harness connection.&nbsp; Wires and DIN connectors used for the wire harness</td></tr><tr><td>Polypropylene Blue Monofilament Sutures</td><td>P8665</td><td>SECUROPRO</td><td>3-0, FS-2, 18&rdquo; reverse cutting suture. Close any surgical incisions</td></tr><tr><td>Retractor cable</td><td>&lrm;0487-013</td><td>West Coast Manufacturing Company</td><td>Industrial Retractable Reel with 48" Stainless Steel Cable, Steel Bracket Back, Chrome Front, 8 oz. Retraction, Plastic Hose Clamp. Pulls excess wire harness up and away from animal</td></tr><tr><td>Rubber gasket</td><td>E-3603 NSF-51</td><td>Saint Gobain</td><td>Clear rubber tubing cut to 1.5 cm width. Gasket for protection/maintaining a seal.&nbsp;</td></tr><tr><td>Small Webb Electrodes</td><td>SWEBB1215</td><td>RhythmLink</td><td>6mm, flat disc [WEBB*] electrode, silver-chloride, wire, Subdermal electrodes</td></tr><tr><td>Sterile Latex Surgical Gloves</td><td>10122</td><td>AmerisourceBergen</td><td>Sterile Latex Gloves, Powder-Free, Donned for sterile surgical technique</td></tr><tr><td>Sterile marker</td><td>DYNJSM01</td><td>Medline</td><td>Sterile Marker, Mark incision/electrode sites&nbsp;</td></tr><tr><td>Sterile surgical drapes with adhesive</td><td>-</td><td>any</td><td>Sterile surgical drapes with adhesive strip on one side, Create a fixed sterile field&nbsp;</td></tr><tr><td>Veterinary anesthesia masks</td><td>-</td><td>any</td><td>Plastic cones with rubber gaskets to seal over rabbit&rsquo;s face. Various sizes. Delivery of inhaled anesthesia</td></tr><tr><td>Veterinary Betadine Surgical Scrub</td><td>NDC 67618-154-16</td><td>Betadine</td><td>Povidone-iodine, 7.5%, Antiseptic Sudsing Skin Cleanser</td></tr><tr><td>Xylazine</td><td>-</td><td>-</td><td>100 mg/mL Xylazine, For sedation</td></tr></tbody></table>

chirurgische implantaatprocedure en bedradingsconfiguratie voor continue langdurige eeg/ecg-monitoring bij konijnen

Surgical Implant Procedure and Wiring Configuration for Continuous Long-Term EEG/ECG Monitoring in Rabbits

Chirurgische implantaatprocedure en bedradingsconfiguratie voor continue langdurige EEG/ECG-monitoring bij konijnen

A multi-system approach that includes the continuous collection of electroencephalogram (EEG) and electrocardiogram (ECG) recordings facilitates a ...

surgical-implant-procedure-wiring-configuration-for-continuous-long

Research

JoVE Journal

Medicine

Geneeskunde

Wij bieden nuttige informatie voor chirurgen die leren het proces van het implanteren van chronische neurale opname elektroden. Technieken voor doordringend oppervlak electrode zijn beschreven in een knaagdier diermodel.

Chirurgische Implantatie van chronische neurale elektroden voor opname Single Unit Activiteit en electrocorticografische signalen

This video demonstrates the procedure to implant EEG-ECG electrodes and attach respiratory probes in a rabbit model for acquiring and analyzing records of its bio signals.

Prepping Conscious Rabbit Model for Simultaneous Video-EEG-ECG-Oximetry-Capnography for Experimental Interventions: A Procedure to Implant EEE-ECG Electrodes and Attach Respiratory Probes in Rabbit Model

In this video, we demonstrate a multisystem monitoring method to evaluate neurological and cardiorespiratory abnormalities during intravenous injection of a seizure-inducing drug. The simultaneous monitoring of video EEG, ECG, oximetry, and capnography parameters helps to understand the real-time physiological changes associated with epileptic seizures.

Multisystem Monitoring of Epileptic Abnormalities in Rabbit: A Method for Simultaneous Video EEG, ECG, Capnography, and Oximetry Recording During Induced Seizures

This video demonstrates a protocol for implanting electrocorticographic electrodes in the mouse cerebral cortex to record electrical signals in response to adeno-associated viruses that target and manipulate cofilin expression.

Implantation of Electrodes into a Mouse Cortex for Electrocorticographic Recordings

Hier demonstreren we het gebruik van een draadloze technologie voor elektro-encefalogram (EEG) in neonatale knaagdiermodellen van menselijke ziekten. Met telemetrie zijn er geen belemmerende verbindingen, waardoor natuurlijk gedrag mogelijk is.

Langdurige Continue EEG-monitoring in kleine knaagdieren modellen van de ziekte bij de mens met behulp van de Epoch draadloze transmitter System

Met behulp van gelijktijdige video-EEG-ECG-oximetrie-capnografie ontwikkelden we een methodologie om de gevoeligheid van konijnenmodellen te evalueren om uitgelokte hartritmestoornissen en aanvallen te ontwikkelen. Dit nieuwe opnamesysteem creëert een platform om de werkzaamheid en veiligheid van therapieën te testen en kan de complexe cascade van multi-systeemgebeurtenissen vastleggen die culmineren in plotselinge dood.

Multi-systeem monitoring voor identificatie van aanvallen, hartritmestoornissen en apneu bij bewuste ingehouden konijnen

Hier presenteren we een methode voor de prestatie- en veiligheidsbeoordeling op lange termijn van zachte subdurale elektrode-arrays in een minivarkensmodel, waarbij chirurgische methoden en hulpmiddelen, postoperatieve magnetische resonantiebeeldvorming, elektrofysiologie van de auditieve cortex, elektrochemische eigenschappen van het implantaat en postmortale immunochemie worden beschreven.

Subdurale zachte elektrocorticografie (ECoG) array-implantatie en langdurige corticale opname bij minivarkens

Mechanismen van plotselinge onverwachte dood bij epilepsie (SUDEP) worden slecht begrepen en zijn moeilijk te vertalen vanuit de huidige modellen. Transgene konijnen kunnen inzicht bieden in deze mechanismen. We beschrijven een methode voor langdurige, continue elektro-encefalografie en elektrocardiografie-opnames in transgene konijnenkits om ernstige gebeurtenissen die tot de dood kunnen leiden te evalueren.

Implantatie van elektro-encefalogram en elektrocardiogram telemetrieapparaten in neonatale konijnenkits

Een zeer reproduceerbaar model voor myocardinfarct bij muizen met minimale invasieve manipulaties wordt beschreven. Het model kan eenvoudig worden uitgevoerd, wat resulteert in een hoge reproduceerbaarheid en overlevingsgraad. Daarom zal het beschreven model het aantal benodigde dieren verminderen zoals gevraagd door het 3R-principe (Vervanging, Verfijning en Vermindering).

Minimaal invasieve chirurgische procedure voor het induceren van Myocardinfarct in Muizen

We presenteren een chirurgische procedure om de intestinale lymfe-lymfeklier bij neonatale varkens te kateteriseren om grote hoeveelheden componenten van het lipidenmetabolisme uit efferente lymfe te verzamelen.

Long-Term catheterisatie van de intestinale Lymfe Stam en verzamelen van lymfe in Neonatale Pigs

Leer een chirurgische implantaatprocedure voor continue EEG/ECG-monitoring bij konijnen. Dit systeem maakt gelijktijdige registratie van hersen- en hartactiviteit mogelijk zonder direct toezicht, wat essentieel is voor onderzoek naar hartritmestoornissen en epileptische aanvallen.

Chirurgische implantaatprocedure en bedradingsconfiguratie voor continue langdurige EEG/ECG-monitoring bij konijnen

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