Name: Structurele biologie en analytische chemie benaderingen voor het karakteriseren van C-glycoside metabole enzymen in de menselijke darmmicrobiota (Video) | JoVE Journal
Uploaded: 2025-05-23T12:50:00.000+00:00
Duration: 13 min 35 s
Description: Ontdek structurele biologie en analytische chemie voor het karakteriseren van C-glycoside metabole enzymen in de menselijke darmmicrobiota. Deze methode integreert eiwitkristallografie, LC-MS/MS en NMR-analyse.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Gomaa, E. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+gut+microbiota%2Fmicrobiome+in+health+and+diseases%3A+a+review%5BTitle%5D)+AND+%22Antonie+Van+Leeuwenhoek%22%5BJournal%5D)">Human gut microbiota/microbiome in health and diseases: a review.</a> Antonie Van Leeuwenhoek. 113 (12), 2019-2040 (2020).</li>
<li>Thursby, E., Juge, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Introduction+to+the+human+gut+microbiota%5BTitle%5D)+AND+%22Biochem+J%22%5BJournal%5D)">Introduction to the human gut microbiota.</a> Biochem J. 474 (11), 1823-1836 (2017).</li>
<li>Nie, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gut+symbionts+alleviate+MASH+through+a+secondary+bile+acid+biosynthetic+pathway%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Gut symbionts alleviate MASH through a secondary bile acid biosynthetic pathway.</a> Cell. 187 (11), 2717-2734.e33 (2024).</li>
<li>Xie, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+flavonoid+O-glycoside%2C+C-glycoside+and+their+aglycones+on+antioxidant+capacity+and+metabolism+during+in+vitro+digestion+and+in+vivo%5BTitle%5D)+AND+%22Foods%22%5BJournal%5D)">Comparison of flavonoid O-glycoside, C-glycoside and their aglycones on antioxidant capacity and metabolism during in vitro digestion and in vivo.</a> Foods. 11 (6), (2022).</li>
<li>Zhang, S. -J., Guo, J. -R., Kang, A., Di, L. -Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advance+in+studies+on+gut+microbiota+in+de-glycosylation+of+traditional+Chinese+medicine+glycosides%5BTitle%5D)+AND+%22Zhongguo+Zhong+yao+za+zhi%3D+Zhongguo+Zhongyao+ZazhiChina+Journal+of+Chinese+Materia+Medica%22%5BJournal%5D)">Advance in studies on gut microbiota in de-glycosylation of traditional Chinese medicine glycosides.</a> Zhongguo Zhong yao za zhi= Zhongguo Zhongyao ZazhiChina Journal of Chinese Materia Medica. 38 (10), 1459-1466 (2013).</li>
<li>Xie, G. -Z., Hui, H. -Y., Peng, M. -J., Tan, Z. -J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biotransformation+of+glycosides+in+herbal+medicine+by+gut+microbiota%5BTitle%5D)+AND+%22World+Chinese+Journal+of+Digestology%22%5BJournal%5D)">Biotransformation of glycosides in herbal medicine by gut microbiota.</a> World Chinese Journal of Digestology. 26 (4), 221(2018).</li>
<li>Vuong, T. V., Wilson, D. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Glycoside+hydrolases%3A+catalytic+base%2Fnucleophile+diversity%5BTitle%5D)+AND+%22Biotechnol+Bioeng%22%5BJournal%5D)">Glycoside hydrolases: catalytic base/nucleophile diversity.</a> Biotechnol Bioeng. 107 (2), 195-205 (2010).</li>
<li>Davies, G., Henrissat, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Structures+and+mechanisms+of+glycosyl+hydrolases%5BTitle%5D)+AND+%22Structure%22%5BJournal%5D)">Structures and mechanisms of glycosyl hydrolases.</a> Structure. 3 (9), 853-859 (1995).</li>
<li>Ito, T., Fujimoto, S., Shimosaka, M., Taguchi, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Production+of+C-glucosides+of+flavonoids+and+related+compounds+by+Escherichia+coli+expressing+buckwheat+C-glucosyltransferase%5BTitle%5D)+AND+%22Plant+Biotechnology%22%5BJournal%5D)">Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase.</a> Plant Biotechnology. 31 (5), 519-524 (2014).</li>
<li>Wei, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Discovery+and+mechanism+of+intestinal+bacteria+in+enzymatic+cleavage+of+C-C+glycosidic+bonds%5BTitle%5D)+AND+%22Appl+Microbiol+Biotechnol%22%5BJournal%5D)">Discovery and mechanism of intestinal bacteria in enzymatic cleavage of C-C glycosidic bonds.</a> Appl Microbiol Biotechnol. 104, 1883-1890 (2020).</li>
<li>Che, Q. M., Akao, T., Hattori, M., Kobashi, K., Namba, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metabolism+of+aloesin+and+related+compounds+by+human+intestinal+bacteria%3A+a+bacterial+cleavage+of+the+C-glucosyl+bond+and+the+subsequent+reduction+of+the+acetonyl+side+chain%5BTitle%5D)+AND+%22Chem+Pharm+Bull+%28Tokyo%29%22%5BJournal%5D)">Metabolism of aloesin and related compounds by human intestinal bacteria: a bacterial cleavage of the C-glucosyl bond and the subsequent reduction of the acetonyl side chain.</a> Chem Pharm Bull (Tokyo). 39 (3), 704-708 (1991).</li>
<li>Wei, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Discovery+and+mechanism+of+intestinal+bacteria+in+enzymatic+cleavage+of+C-C+glycosidic+bonds%5BTitle%5D)+AND+%22Appl+Microbiol+Biotechnol%22%5BJournal%5D)">Discovery and mechanism of intestinal bacteria in enzymatic cleavage of C-C glycosidic bonds.</a> Appl Microbiol Biotechnol. 104 (5), 1883-1890 (2020).</li>
<li>HATTORI, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metabolism+of+barbaloin+by+intestinal+bacteria%5BTitle%5D)+AND+%22Chem+Pharm+Bull+%28Tokyo%29%22%5BJournal%5D)">Metabolism of barbaloin by intestinal bacteria.</a> Chem Pharm Bull (Tokyo). 36 (11), 4462-4466 (1988).</li>
<li>Che, Q. -M., Akao, T., Hattori, M., Kobashi, K., Namba, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+of+a+human+intestinal+bacterium+capable+of+transforming+barbaloin+to+aloe-emodin+anthrone1%5BTitle%5D)+AND+%22Planta+medica%22%5BJournal%5D)">Isolation of a human intestinal bacterium capable of transforming barbaloin to aloe-emodin anthrone1.</a> Planta medica. 57 (01), 15-19 (1991).</li>
<li>Braune, A., Blaut, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deglycosylation+of+puerarin+and+other+aromatic+C-glucosides+by+a+newly+isolated+human+intestinal+bacterium%5BTitle%5D)+AND+%22Environ+Microbiol%22%5BJournal%5D)">Deglycosylation of puerarin and other aromatic C-glucosides by a newly isolated human intestinal bacterium.</a> Environ Microbiol. 13 (2), 482-494 (2011).</li>
<li>Braune, A., Blaut, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intestinal+bacterium+Eubacterium+cellulosolvens+deglycosylates+flavonoid+C-and+O-glucosides%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Intestinal bacterium Eubacterium cellulosolvens deglycosylates flavonoid C-and O-glucosides.</a> Appl Environ Microbiol. 78 (22), 8151-8153 (2012).</li>
<li>Hasanah, U., Miki, K., Nitoda, T., Kanzaki, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aerobic+bioconversion+of+C-glycoside+mangiferin+into+its+aglycone+norathyriol+by+an+isolated+mouse+intestinal+bacterium%5BTitle%5D)+AND+%22Biosci+Biotechnol+Biochem%22%5BJournal%5D)">Aerobic bioconversion of C-glycoside mangiferin into its aglycone norathyriol by an isolated mouse intestinal bacterium.</a> Biosci Biotechnol Biochem. 85 (4), 989-997 (2021).</li>
<li>Kim, D. -H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intestinal+bacterial+metabolism+of+flavonoids+and+its+relation+to+some+biological+activities%5BTitle%5D)+AND+%22Arch+Pharm+Res%22%5BJournal%5D)">Intestinal bacterial metabolism of flavonoids and its relation to some biological activities.</a> Arch Pharm Res. 21, 17-23 (1998).</li>
<li>Kim, M., Lee, J., Han, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deglycosylation+of+isoflavone+C-glycosides+by+newly+isolated+human+intestinal+bacteria%5BTitle%5D)+AND+%22J+Sci+Food+Agric%22%5BJournal%5D)">Deglycosylation of isoflavone C-glycosides by newly isolated human intestinal bacteria.</a> J Sci Food Agric. 95 (9), 1925-1931 (2015).</li>
<li>Tao, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+and+identification+of+Enterococcus+gallinarum+P581a%2C+a+strain+of+intestinal+bacteria+deglycosylating+flavone+C-glycosides%5BTitle%5D)+AND+%22J+Gen+Appl+Microbiol%22%5BJournal%5D)">Isolation and identification of Enterococcus gallinarum P581a, a strain of intestinal bacteria deglycosylating flavone C-glycosides.</a> J Gen Appl Microbiol. 68 (3), 125-133 (2022).</li>
<li>Wang, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+newly+isolated+human+intestinal+strain+deglycosylating+flavonoid+C-glycosides%5BTitle%5D)+AND+%22Arch+Microbiol%22%5BJournal%5D)">A newly isolated human intestinal strain deglycosylating flavonoid C-glycosides.</a> Arch Microbiol. 204 (6), 310(2022).</li>
<li>Xu, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+ultra-performance+liquid+chromatography+coupled+with+quadrupole+time-of-flight+mass+spectrometry+to+determine+the+metabolites+of+orientin+produced+by+human+intestinal+bacteria%5BTitle%5D)+AND+%22J+Chromatogr+B+Analyt+Technol+Biomed+Life+Sci%22%5BJournal%5D)">Application of ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to determine the metabolites of orientin produced by human intestinal bacteria.</a> J Chromatogr B Analyt Technol Biomed Life Sci. 944, 123-127 (2014).</li>
<li>Zheng, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+newly+isolated+human+intestinal+bacterium+strain+capable+of+deglycosylating+flavone+C-glycosides+and+its+functional+properties%5BTitle%5D)+AND+%22Microb+Cell+Fact%22%5BJournal%5D)">A newly isolated human intestinal bacterium strain capable of deglycosylating flavone C-glycosides and its functional properties.</a> Microb Cell Fact. 18, 1-10 (2019).</li>
<li>Braune, A., Engst, W., Blaut, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Identification+and+functional+expression+of+genes+encoding+flavonoid+O-and+C-glycosidases+in+intestinal+bacteria%5BTitle%5D)+AND+%22Environ+Microbiol%22%5BJournal%5D)">Identification and functional expression of genes encoding flavonoid O-and C-glycosidases in intestinal bacteria.</a> Environ Microbiol. 18 (7), 2117-2129 (2016).</li>
<li>Kumano, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((FAD-dependent+C-glycoside-metabolizing+enzymes+in+microorganisms%3A+Screening%2C+characterization%2C+and+crystal+structure+analysis%5BTitle%5D)+AND+%22Proc.+Natl.+Acad.+Sci.+U.S.A%22%5BJournal%5D)">FAD-dependent C-glycoside-metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis.</a> Proc. Natl. Acad. Sci. U.S.A. 118 (40), e2106580118(2021).</li>
<li>Nakamura, K., Zhu, S., Komatsu, K., Hattori, M., Iwashima, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deglycosylation+of+the+isoflavone+C-glucoside+puerarin+by+a+combination+of+two+recombinant+bacterial+enzymes+and+3-oxo-glucose%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Deglycosylation of the isoflavone C-glucoside puerarin by a combination of two recombinant bacterial enzymes and 3-oxo-glucose.</a> Appl Environ Microbiol. 86 (14), e00607-e00620 (2020).</li>
<li>He, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Structural+mechanism+of+a+dual-functional+enzyme+DgpA%2FB%2FC+as+both+a+C-glycoside+cleaving+enzyme+and+an+O-+to+C-glycoside+isomerase%5BTitle%5D)+AND+%22Acta+Pharm+Sin+B%22%5BJournal%5D)">Structural mechanism of a dual-functional enzyme DgpA/B/C as both a C-glycoside cleaving enzyme and an O- to C-glycoside isomerase.</a> Acta Pharm Sin B. 13 (1), 246-255 (2023).</li>
<li>Wang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pro+isomerization+in+MLL1+PHD3-bromo+cassette+connects+H3K4me+readout+to+CyP33+and+HDAC-mediated+repression%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Pro isomerization in MLL1 PHD3-bromo cassette connects H3K4me readout to CyP33 and HDAC-mediated repression.</a> Cell. 141 (7), 1183-1194 (2010).</li>
<li>Mossessova, E., Lima, C. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ulp1-SUMO+crystal+structure+and+genetic+analysis+reveal+conserved+interactions+and+a+regulatory+element+essential+for+cell+growth+in+yeast%5BTitle%5D)+AND+%22Mol+Cell%22%5BJournal%5D)">Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast.</a> Mol Cell. 5 (5), 865-876 (2000).</li>
<li>Delucas, L. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protein+crystallization%3A+virtual+screening+and+optimization%5BTitle%5D)+AND+%22Prog+Biophys+Mol+Biol%22%5BJournal%5D)">Protein crystallization: virtual screening and optimization.</a> Prog Biophys Mol Biol. 88 (3), 285-309 (2005).</li>
<li>Minor, W., Cymborowski, M., Otwinowski, Z., Chruszcz, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((HKL-3000%3A+the+integration+of+data+reduction+and+structure+solution--from+diffraction+images+to+an+initial+model+in+minutes%5BTitle%5D)+AND+%22Acta+Crystallogr+D+Biol+Crystallogr%22%5BJournal%5D)">HKL-3000: the integration of data reduction and structure solution--from diffraction images to an initial model in minutes.</a> Acta Crystallogr D Biol Crystallogr. 62 (Pt 8), 859-866 (2006).</li>
<li>Li, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+efficient+C-glycoside+production+platform+enabled+by+rationally+tuning+the+chemoselectivity+of+glycosyltransferases%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">An efficient C-glycoside production platform enabled by rationally tuning the chemoselectivity of glycosyltransferases.</a> Nat Commun. 15 (1), 8893(2024).</li>
<li>Peters, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+membrane-bound+dehydrogenases+from+Gluconobacter+oxydans+621H+via+whole-cell+activity+assays+using+multideletion+strains%5BTitle%5D)+AND+%22Appl+Microbiol+Biotechnol%22%5BJournal%5D)">Characterization of membrane-bound dehydrogenases from Gluconobacter oxydans 621H via whole-cell activity assays using multideletion strains.</a> Appl Microbiol Biotechnol. 97 (14), 6397-6412 (2013).</li>
<li>Nasseri, S. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+alternative+broad-specificity+pathway+for+glycan+breakdown+in+bacteria%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">An alternative broad-specificity pathway for glycan breakdown in bacteria.</a> Nature. 631 (8019), 199-206 (2024).</li>
<li>Rose, J. P., Wang, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((SAD+phasing%3A+History%2C+current+impact+and+future+opportunities%5BTitle%5D)+AND+%22Arch+Biochem+Biophys%22%5BJournal%5D)">SAD phasing: History, current impact and future opportunities.</a> Arch Biochem Biophys. 602, 80-94 (2016).</li>
<li>Abramson, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Accurate+structure+prediction+of+biomolecular+interactions+with+AlphaFold+3%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Accurate structure prediction of biomolecular interactions with AlphaFold 3.</a> Nature. 630 (8016), 493-500 (2024).</li>
<li>Taberman, H., Parkkinen, T., Rouvinen, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Structural+and+functional+features+of+the+NAD%28P%29+dependent+Gfo%2FIdh%2FMocA+protein+family+oxidoreductases%5BTitle%5D)+AND+%22Protein+Sci%22%5BJournal%5D)">Structural and functional features of the NAD(P) dependent Gfo/Idh/MocA protein family oxidoreductases.</a> Protein Sci. 25 (4), 778-786 (2016).</li>
<li>Jahn, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Understanding+the+chemistry+of+the+artificial+electron+acceptors+PES%2C+PMS%2C+DCPIP+and+Wurster%27s+Blue+in+methanol+dehydrogenase+assays%5BTitle%5D)+AND+%22J.+Biol.+Inorg.+Chem%22%5BJournal%5D)">Understanding the chemistry of the artificial electron acceptors PES, PMS, DCPIP and Wurster's Blue in methanol dehydrogenase assays.</a> J. Biol. Inorg. Chem. 25, 199-212 (2020).</li>
<li>Yu, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biotransformation+of+puerarin+into+puerarin-6+%E2%80%B3-O-phosphate+by+Bacillus+cereus%5BTitle%5D)+AND+%22J+Ind+Microbiol+Biotechnol%22%5BJournal%5D)">Biotransformation of puerarin into puerarin-6 ″-O-phosphate by Bacillus cereus.</a> J Ind Microbiol Biotechnol. 39 (2), 299-305 (2012).</li>
<li>Lin, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lysineless+HiBiT+and+NanoLuc+tagging+systems+as+alternative+tools+for+monitoring+targeted+protein+degradation%5BTitle%5D)+AND+%22ACS+Med+Chem+Lett%22%5BJournal%5D)">Lysineless HiBiT and NanoLuc tagging systems as alternative tools for monitoring targeted protein degradation.</a> ACS Med Chem Lett. 15 (8), 1367-1375 (2024).</li>
<li>Ma, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+Widespread+Radical-Mediated+Glycolysis+Pathway%5BTitle%5D)+AND+%22J+Am+Chem+Soc%22%5BJournal%5D)">A Widespread Radical-Mediated Glycolysis Pathway.</a> J Am Chem Soc. 146 (38), 26187-26197 (2024).</li>
<li>Yang, L., Lei, S., Xu, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rising+above%3A+exploring+the+therapeutic+potential+of+natural+product-based+compounds+in+human+cancer+treatment%5BTitle%5D)+AND+%22Tradit+Med+Res%22%5BJournal%5D)">Rising above: exploring the therapeutic potential of natural product-based compounds in human cancer treatment.</a> Tradit Med Res. 10 (3), 18(2025).</li>
<li>Zhu, C., Cook, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+concise+synthesis+of+%28%2B%29-artemisinin%5BTitle%5D)+AND+%22J+Am+Chem+Soc%22%5BJournal%5D)">A concise synthesis of (+)-artemisinin.</a> J Am Chem Soc. 134 (33), 13577-13579 (2012).</li>
<li>Schade, A. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((AKT+and+EZH2+inhibitors+kill+TNBCs+by+hijacking+mechanisms+of+involution%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">AKT and EZH2 inhibitors kill TNBCs by hijacking mechanisms of involution.</a> Nature. , 1-9 (2024).</li>
<li>Karpinska, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Selective+degradation+of+MLK3+by+a+novel+CEP1347-VHL-02+PROTAC+compound+limits+the+oncogenic+potential+of+TNBC%5BTitle%5D)+AND+%22J+Med+Chem%22%5BJournal%5D)">Selective degradation of MLK3 by a novel CEP1347-VHL-02 PROTAC compound limits the oncogenic potential of TNBC.</a> J Med Chem. 67 (17), 15012-15028 (2024).</li>
</ol>

<table><tbody><tr><td>2,6-dichloorindoofenol (DCPIP)</td><td>Solarbio Science and Technology</td><td>D6350</td><td></td></tr><tr><td>2-Mercaptoethanol(&beta;-ME)</td><td>Sigma-Aldrich</td><td>08168</td><td></td></tr><tr><td>&nbsp; 5 &times; Protein Loading Dye</td><td>Sangon Biotech</td><td>C508320</td><td></td></tr><tr><td>Azijnzuur</td><td>Shanghai MACKLIN</td><td>A111247</td><td></td></tr><tr><td>Acetonitril</td><td>Thermo Fisher Scientific&trade;?Invitrogen</td><td>A12147</td><td></td></tr><tr><td>Bruker Avance III HD 700 MHz NMR Spectrometer</td><td>Bruker</td><td>AVANCE III HD 700MHz</td><td></td></tr><tr><td>Bruker Ultrashield 400 Plus 400MHz</td><td>Bruker</td><td>AVANCE III 400 MHz</td><td></td></tr><tr><td>Centrifuge 5424R</td><td>Eppendorf</td><td>022620601</td><td></td></tr><tr><td>Chirascan&trade; plus CD spectrometer</td><td>Applied Photophysics</td><td>Chirascan&trade;&nbsp;</td><td></td></tr><tr><td>Daidzeïne</td><td>Shanghai Yuanye Bio-Technology</td><td>B20227</td><td></td></tr><tr><td>Daidzin</td><td>Shanghai Yuanye Bio-Technology</td><td>B20226</td><td></td></tr><tr><td>Dithiothreitol(DTT)</td><td>Solarbio Science and Technology</td><td>D8220</td><td></td></tr><tr><td>E. coli BL21 (DE3) competent cellen</td><td>Thermo Fisher Scientific&trade;?Invitrogen</td><td>EC0114</td><td></td></tr><tr><td>Genisteïne</td><td>Shanghai Yuanye Bio-Technology</td><td>S31565</td><td></td></tr><tr><td>Genisteïne-8-C-glucoside</td><td>Shanghai Yuanye Bio-Technology</td><td>BP2095</td><td></td></tr><tr><td>Genistin</td><td>Shanghai Yuanye Bio-Technology</td><td>S31591</td><td></td></tr><tr><td>Glucose</td><td>Sigma-Aldrich</td><td>G8270</td><td></td></tr><tr><td>GraphPad Prism 9</td><td>GraphPad Software</td><td>Version?Prism 9</td><td>Bereken de Km en kcat kinetische parameters</td></tr><tr><td>HiLoad Superdex 200 pg preparatieve SEC kolommen</td><td>Cytiva</td><td>28989335</td><td></td></tr><tr><td>HisTrap HP His tag eiwitzuiveringskolommen</td><td>Cytiva</td><td>17524802</td><td></td></tr><tr><td>HiTrap Q FF anionenuitwisselingschromatografiekolom</td><td>Cytiva</td><td>17515601</td><td></td></tr><tr><td>HKL-3000</td><td>HKL research Inc</td><td>&nbsp;versie 723</td><td></td></tr><tr><td>HPLC kolom TC-C18(2), 170&Aring;, 5 &micro;m, 4.6 x 250 mm</td><td>Agilent Technologies</td><td>518925-902</td><td></td></tr><tr><td>Isopropyl &beta;-D-1-thiogalactopyranosid?IPTG)</td><td>Sigma-Aldrich</td><td>PHG0010</td><td></td></tr><tr><td>Lactose</td><td>Sigma-Aldrich</td><td>17814</td><td></td></tr><tr><td>LC-20AT, hogeprestatievloeistofchromatografie</td><td>Shimadzu</td><td>LC20AT</td><td></td></tr><tr><td>Maltose</td><td>Sigma-Aldrich</td><td>47288</td><td></td></tr><tr><td>Maltotriose</td><td>Sigma-Aldrich</td><td>443713</td><td></td></tr><tr><td>Manganchloride</td><td>Shanghai Yuanye Bio-Technology</td><td>S31112</td><td></td></tr><tr><td>Methanol</td><td>Thermo Fisher Scientific&trade;?Invitrogen</td><td>M23142</td><td></td></tr><tr><td>NAD+</td><td>Solarbio Science and Technology</td><td>N8110</td><td></td></tr><tr><td>Olympus SZX12 Stereo Microscoop</td><td>OLYMPUS&nbsp;</td><td>SZX7</td><td></td></tr><tr><td>fenylmethylsulfonylfluoride (PMSF)</td><td>Sigma-Aldrich</td><td>P7626&nbsp;</td><td></td></tr><tr><td>Phenix</td><td>The Phenix development team</td><td>1.21.2</td><td>Phenix is een uitgebreide softwaresuite voor eiwitkristallenstructuurbepaling, die SAD (Single-wavelength Anomalous Dispersion) en MR (Molecular Replacement) methoden met Phaser-software omvat.</td></tr><tr><td>Preparatief hogeprestatievloeistofchromatograaf</td><td>Waters</td><td>2545/2998</td><td></td></tr><tr><td>Proteïnekristallisatiekit</td><td>Hampton Research</td><td>HR2-110/HR2-112/HR2-144/HR2-126/HR2-098</td><td></td></tr><tr><td>Puerarine</td><td>Shanghai Yuanye Bio-Technology</td><td>B20446</td><td></td></tr><tr><td>Schudincubator</td><td>Shanghai Zhichu Instrument Co.,Ltd</td><td>&nbsp;ZQZY-AF8</td><td></td></tr><tr><td>SnakeSkin&trade; dialysemembraan, 10 kDa MWCO, 35 mm</td><td>Thermo Fisher Scientific&trade;?Invitrogen</td><td>88245</td><td></td></tr><tr><td>Oplosbare zetmeel</td><td>Sigma-Aldrich</td><td>S9765</td><td></td></tr><tr><td>Sorvall LYNX 6000 Superspeed Centrifuge</td><td>Thermo Fisher Scientific&trade;</td><td>75006590</td><td></td></tr><tr><td>SpectraMax i3x Multi-Mode Microplate Reader</td><td>MOLECULAR DEVICES</td><td>SpectraMax i3x&nbsp;</td><td></td></tr><tr><td>SpectraMax QuickDrop Micro-Volume Spectrofotometer Specificaties</td><td>MOLECULAR DEVICES</td><td>QuickDrop</td><td></td></tr><tr><td>Sucrose</td><td>Sigma-Aldrich</td><td>S5016</td><td></td></tr><tr><td>SunFire C18 OBD Prep Column</td><td>Waters</td><td>186002741</td><td></td></tr><tr><td>Thermo Fisher Scientific Q EXACTIVE</td><td>Thermo Fisher Scientific&trade;?Invitrogen</td><td>IQLAAEGAAPFALGMBDK</td><td></td></tr><tr><td>Ubiquitine-achtige protease 1</td><td>Thermo Fisher Scientific&trade;?Invitrogen</td><td>12588018</td><td></td></tr><tr><td>Ultra laag temperatuurvriezer</td><td>Thermo Fisher Scientific</td><td>905GP-ULTS</td><td></td></tr><tr><td>Vakuumcentrifugaal concentrator</td><td>Beijing JM Technology</td><td>CV600</td><td></td></tr><tr><td>Vitexine</td><td>Sigma-Aldrich</td><td>49513</td><td></td></tr></tbody></table>

structurele biologie en analytische chemie benaderingen voor het karakteriseren van c-glycoside metabole enzymen in de menselijke darmmicrobiota

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Structurele biologie en analytische chemie benaderingen voor het karakteriseren van C-glycoside metabole enzymen in de menselijke darmmicrobiota

The gut microbiota has emerged as a key modulator of host health, particularly because of its capacity to metabolize dietary glycosides. While ...

structural-biology-analytical-chemistry-approaches-for-characterizing

Research

JoVE Journal

Biochemistry

Biochemie

Een specifieke en gevoelige methode om inzicht te krijgen in de expressie profiel van glycosfingolipiden antigenen in het immuunsysteem organen en cellen wordt beschreven. De methode maakt gebruik van de ion trap massaspectrometrie waardoor stapsgewijze versnippering van glycosfingolipiden moleculen voor structurele analyse in vergelijking met chemisch gesynthetiseerde standaard.

Massaspectrometrische analyse van glycosfingolipiden antigenen

Source: Hu, Y. et.al., Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems. J. Vis. Exp. (2019)This video demonstrates the analysis of short-chain fatty acid production by human gut microbiota in response to exopolysaccharides. This highlights microbial fermentation products separation and quantification using gas chromatography. The results reveal that exopolysaccharides modulate microbial metabolites that influence host-pathogen...

Investigating the Human Gut Microbial Activity on Exopolysaccharides

De actieve bacteriegemeenschappen geassocieerd met de darm van Spodoptera littoralis, werd bepaald door stabiele-isotoop probing (SIP) gekoppeld pyrosequencing. Met behulp van deze methodiek, de identificatie van de metabolisch actieve bacteriën soorten binnen de gemeenschap werd gedaan met een hoge resolutie en nauwkeurigheid.

Identificatie van metabool actieve bacteriën in de darm van de Generalist<em&gt; Spodoptera littoralis</em&gt; Via DNA Stabiele Isotopen Onderzoek naar gebruik van<sup&gt; 13</sup&gt; C-Glucose

Een high-throughput assay voor enzymscreening wordt beschreven. Deze multiplexed, gebruiksklare assaykit bestaat uit vooraf gekozen Chromogene Polymer Hydrogel (CPH) substraten en complexe Insoluble Chromogene Biomass (ICB) substraten. Doelenzym zijn polysacharide-afbrekende endo-enzymen en proteasen.

High-throughput screening van Carbohydrate-afbrekende enzymen via innovatieve Onoplosbare chromogeen substraat Assay Kits

Hier presenteren we een protocol voor het kweken van de darmflora van de dikke darm in vitro, met behulp van een reeks van bioreactoren die de fysiologische omstandigheden van de gastro-intestinale tractus simuleren.

Toepassing van geavanceerde In Vitro kweken van technologie om te bestuderen van de menselijke darmflora

Hier beschrijven we de high-throughput detectie en kwantificering van fucosylated menselijke melk oligosacchariden (zorgorganisaties) met behulp van een geheel-cel biosensor. We ook laten zien hier, de aanpassing van dit platform naar analyse van HMO productie stammen, gericht op het verbeteren van de signaal / ruisverhouding.

Analyse van de Fucosylated mens melk trisacharide in biotechnologische Context, waarbij het genetisch gecodeerd biosensoren

Hier beschreven is een protocol om de interacties tussen endobiotica en menselijke gut microbiota met behulp van in vitro batch fermentatiesystemen te onderzoeken.

Analyse van interacties tussen Endobiotica en humane gut microbiota met behulp van in-vitro Bad fermentatiesystemen

Hier beschreven is een vereenvoudigde standaard werkings procedure voor microbiome profilering met behulp van 16S rRNA metagenomic sequencing en analyse met behulp van vrij beschikbare instrumenten. Dit protocol zal onderzoekers die nieuw zijn in het microbiome veld en degenen die updates vereisen over methoden om bacteriële profilering bij een hogere resolutie te bereiken helpen.

Microbiota-analyse met behulp van twee-stap PCR en de volgende generatie 16S rRNA Gensequencing

Open zoeken maakt de identificatie van glycopeptiden versierd met voorheen onbekende glycaansamenstellingen mogelijk. In dit artikel wordt een gestroomlijnde aanpak voor het uitvoeren van open zoekopdrachten en daaropvolgende glycaangerichte glycopeptide-zoekopdrachten gepresenteerd voor bacteriële monsters met behulp van Acinetobacter baumannii als model.

De toepassing van open zoekgebaseerde benaderingen voor de identificatie van Acinetobacter baumannii O-gebonden glycopeptiden

Deze studie stelt een reeks standaard operationele procedures (SOP's) vast voor het efficiënt screenen en isoleren van darmbacteriën die in staat zijn om C-glycosiden te splitsen.

Screening en isolatie van C-glycoside-splitsende darmbacteriën

Ontdek structurele biologie en analytische chemie voor het karakteriseren van C-glycoside metabole enzymen in de menselijke darmmicrobiota. Deze methode integreert eiwitkristallografie, LC-MS/MS en NMR-analyse.

Structurele biologie en analytische chemie benaderingen voor het karakteriseren van C-glycoside metabole enzymen in de menselijke darmmicrobiota (Video) | JoVE Journal

Structurele biologie en analytische chemie benaderingen voor het karakteriseren van C-glycoside metabole enzymen in de menselijke darmmicrobiota

Meer video's verkennen