Name: 2.5D-model voor ex vivo mechanische karakterisering van kiemende angiogenese in levend weefsel (Video) | JoVE Journal
Uploaded: 2025-02-28T13:10:00.000+00:00
Duration: 10 min
Description: Verken een 2.5D ex vivo model voor mechanische karakterisering van angiogenese in varkenscarotis. Deze methode combineert tractiekrachtmicroscopie met fysiologisch relevant weefselonderzoek.

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<meta charset="utf8"></head><body>Veelzijdig 2.5D-model voor het analyseren van cellulaire kieming in varkensslagaders

<ol><li>Carmeliet, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Angiogenesis+in+health+and+disease%5BTitle%5D)+AND+%22Nat+Med%22%5BJournal%5D)">Angiogenesis in health and disease.</a> Nat Med. 9 (6), 653-660 (2003).</li>
<li>Folkman, J. Angiogenesis in cancer, vascular, rheumatoid and other disease. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aFolkman%2C+J+%22Nat+Med%22">Nat Med</a>. 1, 27-30 (1995).</li>
<li>Kretschmer, M., Rüdiger, D., Zahler, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanical+aspects+of+angiogenesis%5BTitle%5D)+AND+%22Cancers%22%5BJournal%5D)">Mechanical aspects of angiogenesis.</a> Cancers. 13 (19), 4987(2021).</li>
<li>Bordeleau, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Matrix+stiffening+promotes+a+tumor+vasculature+phenotype%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Matrix stiffening promotes a tumor vasculature phenotype.</a> Proc Natl Acad Sci U S A. 114 (3), 492-497 (2017).</li>
<li>Blanco, R., Gerhardt, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((VEGF+and+Notch+in+tip+and+stalk+cell+selection%5BTitle%5D)+AND+%22Cold+Spring+Harb+Perspect+Med%22%5BJournal%5D)">VEGF and Notch in tip and stalk cell selection.</a> Cold Spring Harb Perspect Med. 3 (1), a006569(2013).</li>
<li>Adams, R. H., Alitalo, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Molecular+regulation+of+angiogenesis+and+lymphangiogenesis%5BTitle%5D)+AND+%22Nat+Rev+Mol+Cell+Biol%22%5BJournal%5D)">Molecular regulation of angiogenesis and lymphangiogenesis.</a> Nat Rev Mol Cell Biol. 8, 464-478 (2007).</li>
<li>Carmeliet, P., De Smet, F., Loges, S., Mazzone, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Branching+morphogenesis+and+antiangiogenesis+candidates%3A+tip+cells+lead+the+way%5BTitle%5D)+AND+%22Nat+Rev+Clin+Oncol%22%5BJournal%5D)">Branching morphogenesis and antiangiogenesis candidates: tip cells lead the way.</a> Nat Rev Clin Oncol. 6, 315-326 (2009).</li>
<li>Salam, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+migration+of+human+mscs+through+fibrin+hydrogels+as+a+tool+for+formulation+optimisation%5BTitle%5D)+AND+%22Materials%22%5BJournal%5D)">Assessment of migration of human mscs through fibrin hydrogels as a tool for formulation optimisation.</a> Materials. 11 (9), 1781(2018).</li>
<li>Solbu, A. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+cell+migration+in+hydrogels%3A+An+overview+of+relevant+materials+and+methods%5BTitle%5D)+AND+%22Materials+Today+Bio%22%5BJournal%5D)">Assessing cell migration in hydrogels: An overview of relevant materials and methods.</a> Materials Today Bio. 18, 100537(2023).</li>
<li>Cao, W., Li, X., Zuo, X., Gao, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Migration+of+endothelial+cells+into+photo-responsive+hydrogels+with+tunable+modulus+under+the+presence+of+pro-inflammatory+macrophages%5BTitle%5D)+AND+%22Regenerat+Biomater%22%5BJournal%5D)">Migration of endothelial cells into photo-responsive hydrogels with tunable modulus under the presence of pro-inflammatory macrophages.</a> Regenerat Biomater. 6 (5), 259-267 (2019).</li>
<li>Staton, C. A., Reed, M. W. R., Brown, N. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+critical+analysis+of+current+in+vitro+and+in+vivo+angiogenesis+assays%5BTitle%5D)+AND+%22Int+J+Exp+Pathol%22%5BJournal%5D)">A critical analysis of current in vitro and in vivo angiogenesis assays.</a> Int J Exp Pathol. 90 (3), 195-221 (2009).</li>
<li>Staton, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Current+methods+for+assaying+angiogenesis+in+vitro+and+in+vivo%5BTitle%5D)+AND+%22Int+J+Exp+Path%22%5BJournal%5D)">Current methods for assaying angiogenesis in vitro and in vivo.</a> Int J Exp Path. 85, 233-248 (2004).</li>
<li>Ngo, T. X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+Vitro+models+for+angiogenesis+research%3A+A+review%5BTitle%5D)+AND+%22Int+J+Tissue+Regenerat%22%5BJournal%5D)">In Vitro models for angiogenesis research: A review.</a> Int J Tissue Regenerat. 5, 37-45 (2014).</li>
<li>Tomita, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+ex+vivo+choroid+sprouting+assay+of+ocular+microvascular+angiogenesis%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">An ex vivo choroid sprouting assay of ocular microvascular angiogenesis.</a> J Vis Exp. (162), e61677(2020).</li>
<li>Kapoor, A., Chen, C. G., Iozzo, R. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+simplified+aortic+ring+assay%3A+A+useful+ex+vivo+method+to+assess+biochemical+and+functional+parameters+of+angiogenesis%5BTitle%5D)+AND+%22Matrix+Biol+Plus%22%5BJournal%5D)">A simplified aortic ring assay: A useful ex vivo method to assess biochemical and functional parameters of angiogenesis.</a> Matrix Biol Plus. 6-7, 100025(2020).</li>
<li>Stiffey-Wilusz, J., Boice, J. A., Ronan, J., Fletcher, A. M., Anderson, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+ex+vivo+angiogenesis+assay+utilizing+commercial+porcine+carotid+artery%3A+Modification+of+the+rat+aortic+ring+assay%5BTitle%5D)+AND+%22Angiogenesis%22%5BJournal%5D)">An ex vivo angiogenesis assay utilizing commercial porcine carotid artery: Modification of the rat aortic ring assay.</a> Angiogenesis. 4 (1), 3-9 (2001).</li>
<li>Kniazeva, E., Putnam, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Endothelial+cell+traction+and+ECM+density+influence+both+capillary+morphogenesis+and+maintenance+in+3-D%5BTitle%5D)+AND+%22Am+J+Physiol+Cell+Physiol%22%5BJournal%5D)">Endothelial cell traction and ECM density influence both capillary morphogenesis and maintenance in 3-D.</a> Am J Physiol Cell Physiol. 297 (1), C179-C187 (2009).</li>
<li>Davidson, C. D., Wang, W. Y., Zaimi, I., Jayco, D. K. P., Baker, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cell+force-mediated+matrix+reorganization+underlies+multicellular+network+assembly%5BTitle%5D)+AND+%22Sc+Rep%22%5BJournal%5D)">Cell force-mediated matrix reorganization underlies multicellular network assembly.</a> Sc Rep. 9 (1), 12(2019).</li>
<li>Lyle, K. S., Corleto, J. A., Wittmann, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microtubule+dynamics+regulation+contributes+to+endothelial+morphogenesis%5BTitle%5D)+AND+%22BioArchitecture%22%5BJournal%5D)">Microtubule dynamics regulation contributes to endothelial morphogenesis.</a> BioArchitecture. 2 (6), 220-227 (2012).</li>
<li>Kniazeva, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantification+of+local+matrix+deformations+and+mechanical+properties+during+capillary+morphogenesis+in+3D%5BTitle%5D)+AND+%22Integrat+Biol%22%5BJournal%5D)">Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D.</a> Integrat Biol. 4 (4), 431-439 (2012).</li>
<li>Quintanilla, M. A., Hammer, J. A., Beach, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-muscle+myosin+2+at+a+glance%5BTitle%5D)+AND+%22J+Cell+Sci%22%5BJournal%5D)">Non-muscle myosin 2 at a glance.</a> J Cell Sci. 136 (5), jcs.260890(2023).</li>
<li>Fischer, R. S., Gardel, M., Ma, X., Adelstein, R. S., Waterman, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Local+cortical+tension+by+myosin+II+guides+3D+endothelial+cell+branching%5BTitle%5D)+AND+%22Curr+Biol%22%5BJournal%5D)">Local cortical tension by myosin II guides 3D endothelial cell branching.</a> Curr Biol. 19 (3), 260-265 (2009).</li>
<li>Yoon, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Myosin+IIA%E2%80%93mediated+forces+regulate+multicellular+integrity+during+vascular+sprouting%5BTitle%5D)+AND+%22Mol+Biol+Cell%22%5BJournal%5D)">Myosin IIA–mediated forces regulate multicellular integrity during vascular sprouting.</a> Mol Biol Cell. 30 (16), 1974-1984 (2019).</li>
<li>Du, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+characterization+of+mechanical+interactions+between+endothelial+cells+and+extracellular+matrix+during+angiogenic+sprouting%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Three-dimensional characterization of mechanical interactions between endothelial cells and extracellular matrix during angiogenic sprouting.</a> Sci Rep. 6, 21362(2016).</li>
<li>Vaeyens, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Matrix+deformations+around+angiogenic+sprouts+correlate+to+sprout+dynamics+and+suggest+pulling+activity%5BTitle%5D)+AND+%22Angiogenesis%22%5BJournal%5D)">Matrix deformations around angiogenic sprouts correlate to sprout dynamics and suggest pulling activity.</a> Angiogenesis. 23 (3), 315-324 (2020).</li>
<li>Style, R. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traction+force+microscopy+in+physics+and+biology%5BTitle%5D)+AND+%22Soft+Matter%22%5BJournal%5D)">Traction force microscopy in physics and biology.</a> Soft Matter. 10 (23), 4047-4055 (2014).</li>
<li>Trepat, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Physical+forces+during+collective+cell+migration%5BTitle%5D)+AND+%22Nat+Phys%22%5BJournal%5D)">Physical forces during collective cell migration.</a> Nat Phys. 5 (6), 426-430 (2009).</li>
<li>Santos-Oliveira, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+force+at+the+tip+-+modelling+tension+and+proliferation+in+sprouting+angiogenesis%5BTitle%5D)+AND+%22PLoS+Comput+Biol%22%5BJournal%5D)">The force at the tip - modelling tension and proliferation in sprouting angiogenesis.</a> PLoS Comput Biol. 11 (8), e1004436(2015).</li>
<li>Boreddy, S. R., Sahu, R. P., Srivastava, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Benzyl+isothiocyanate+suppresses+pancreatic+tumor+angiogenesis+and+invasion+by+inhibiting+HIF-%CE%B1%2FVEGF%2FRho-GTPases%3A+Pivotal+role+of+STAT-3%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Benzyl isothiocyanate suppresses pancreatic tumor angiogenesis and invasion by inhibiting HIF-α/VEGF/Rho-GTPases: Pivotal role of STAT-3.</a> PLoS One. 6 (10), 0025799(2011).</li>
<li>Teng, R. J., Eis, A., Bakhutashvili, I., Arul, N., Konduri, G. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Increased+superoxide+production+contributes+to+the+impaired+angiogenesis+of+fetal+pulmonary+arteries+with+in+utero+pulmonary+hypertension%5BTitle%5D)+AND+%22Am+J+Physiol+Lung+Cell+Mol+Physiol%22%5BJournal%5D)">Increased superoxide production contributes to the impaired angiogenesis of fetal pulmonary arteries with in utero pulmonary hypertension.</a> Am J Physiol Lung Cell Mol Physiol. 297 (1), L184-L195 (2009).</li>
<li>Costa, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Asymmetric+division+coordinates+collective+cell+migration+in+angiogenesis%5BTitle%5D)+AND+%22Nature+Cell+Biol%22%5BJournal%5D)">Asymmetric division coordinates collective cell migration in angiogenesis.</a> Nature Cell Biol. 18 (12), 1292-1301 (2016).</li>
<li>Slater, K., Partridge, J., Nandivada, H. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSlater%2C+K+%22Corning+tuning+the+elastic+moduli+of+Corning+Matrigel+and+collagen+I+3D+matrices+by+varying+the+protein+concentration%22">Corning tuning the elastic moduli of Corning Matrigel and collagen I 3D matrices by varying the protein concentration</a>. , Corning Incorporated, Life Sciences, MA USA. (2019).</li>
<li>Lee, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+chain+flexibility+on+cell+adhesion%3A+Semi-flexible+model-based+analysis+of+cell+adhesion+to+hydrogels%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Effect of chain flexibility on cell adhesion: Semi-flexible model-based analysis of cell adhesion to hydrogels.</a> Sci Rep. 9 (1), 2463(2019).</li>
<li>Motte, S., Kaufman, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Strain+stiffening+in+collagen+i+networks%5BTitle%5D)+AND+%22Biopolymers%22%5BJournal%5D)">Strain stiffening in collagen i networks.</a> Biopolymers. 99 (1), 35-46 (2013).</li>
<li>Butcher, D. T., Alliston, T., Weaver, V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+tense+situation%3A+Forcing+tumour+progression%5BTitle%5D)+AND+%22Nat+Rev+Cancer%22%5BJournal%5D)">A tense situation: Forcing tumour progression.</a> Nat Rev Cancer. 9 (2), 108-122 (2009).</li>
<li>Artym, V. V., Matsumoto, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Imaging+cells+in+three-dimensional+collagen+matrix%5BTitle%5D)+AND+%22Curr+Prot+Cell+Biol%22%5BJournal%5D)">Imaging cells in three-dimensional collagen matrix.</a> Curr Prot Cell Biol. 10, Unit 10.18.1-Unit 10.18.20 (2010).</li>
<li>Labernadie, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+mechanically+active+heterotypic+E-cadherin%2FN-cadherin+adhesion+enables+fibroblasts+to+drive+cancer+cell+invasion%5BTitle%5D)+AND+%22Nat+Cell+Biol%22%5BJournal%5D)">A mechanically active heterotypic E-cadherin/N-cadherin adhesion enables fibroblasts to drive cancer cell invasion.</a> Nat Cell Biol. 19 (3), 224-237 (2017).</li>
<li>Bazellières, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Control+of+cell-cell+forces+and+collective+cell+dynamics+by+the+intercellular+adhesome%5BTitle%5D)+AND+%22Nat+Cell+Biol%22%5BJournal%5D)">Control of cell-cell forces and collective cell dynamics by the intercellular adhesome.</a> Nat Cell Biol. 17 (4), 409-420 (2015).</li>
<li>Uroz, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traction+forces+at+the+cytokinetic+ring+regulate+cell+division+and+polyploidy+in+the+migrating+zebrafish+epicardium%5BTitle%5D)+AND+%22Nat+Mater%22%5BJournal%5D)">Traction forces at the cytokinetic ring regulate cell division and polyploidy in the migrating zebrafish epicardium.</a> Nat Mater. 18 (9), 1015-1023 (2019).</li>
<li>Dong, C., Nastaran, Z., Konstantopoulos, K. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aDong%2C+C+%22Biomechanics+in+oncology%22">Biomechanics in oncology</a>. , Springer Nature. Switzerland, AG. (2018).</li>
<li>Schwarz, U. S., Soiné, J. R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Traction+force+microscopy+on+soft+elastic+substrates%3A+A+guide+to+recent+computational+advances%5BTitle%5D)+AND+%22Biochim+Biophys+Acta%22%5BJournal%5D)">Traction force microscopy on soft elastic substrates: A guide to recent computational advances.</a> Biochim Biophys Acta. 1853 (11), 3095-3104 (2015).</li>
<li>Toyjanova, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+Viscoelastic+traction+force+microscopy%5BTitle%5D)+AND+%22Soft+Matter%22%5BJournal%5D)">3D Viscoelastic traction force microscopy.</a> Soft Matter. 10 (40), 8095-8106 (2014).</li>
<li>Legant, W. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multidimensional+traction+force+microscopy+reveals+out-of-plane+rotational+moments+about+focal+adhesions%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Multidimensional traction force microscopy reveals out-of-plane rotational moments about focal adhesions.</a> Proc Natl Acad Sci U S A. 110 (3), 881-886 (2013).</li>
<li>Steinwachs, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+force+microscopy+of+cells+in+biopolymer+networks%5BTitle%5D)+AND+%22Nat+Meth%22%5BJournal%5D)">Three-dimensional force microscopy of cells in biopolymer networks.</a> Nat Meth. 13 (2), 171-176 (2016).</li>
<li>Franck, C., Maskarinec, S. A., Tirrell, D. A., Ravichandran, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+traction+force+microscopy%3A+A+new+tool+for+quantifying+cell-matrix+interactions%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Three-dimensional traction force microscopy: A new tool for quantifying cell-matrix interactions.</a> PLoS One. 6 (3), e17833(2011).</li>
</ol>

<table><tbody><tr><td>2% bis-acrylamide</td><td></td><td>Bio-Rad</td><td>1610143</td></tr><tr><td>2-mercaptoethanol</td><td></td><td>Merck Life Science</td><td>60-24-2</td></tr><tr><td>3-(Trimethoxysilyl)propyl methacrylate</td><td>Bind-Silane</td><td>Sigma-Aldrich</td><td>440159-100ML</td></tr><tr><td>40% acrylamide</td><td></td><td>Bio-Rad</td><td>1610140</td></tr><tr><td>Aboslute ethanol (for analysis)</td><td></td><td>VWR International</td><td>1.00983.1000</td></tr><tr><td>Absolute ethanol (industrial)</td><td></td><td>VWR International</td><td>83813.41</td></tr><tr><td>Acetic acid, glacial 100%</td><td></td><td>Merck</td><td>1000562500</td></tr><tr><td>Ammonium persulfate</td><td>APS</td><td>Bio-Rad</td><td>7727-54-0</td></tr><tr><td>antibody (primary) - calponin</td><td></td><td>abcam</td><td>ab46794</td></tr><tr><td>antibody (primary) - CD31</td><td></td><td>Serotec</td><td>MCA1746</td></tr><tr><td>antibody (primary) - &alpha;-smooth muscle actin</td><td>&alpha;SMA</td><td>Dako</td><td>M0851</td></tr><tr><td>antibody (secondary) - goat-anti-mouse-IgG1 Alexa 488</td><td></td><td>Molecular Probes</td><td>A21121</td></tr><tr><td>antibody (secondary) - goat-anti-mouse-IgG2a Alexa 555</td><td></td><td>Molecular Probes</td><td>A21137</td></tr><tr><td>antibody (secondary) - goat-anti-rabbit-IgG Alexa 555</td><td></td><td>Molecular Probes</td><td>A21428</td></tr><tr><td>Autoclave</td><td></td><td>Astell</td><td></td></tr><tr><td>Calcium chloride dihydrate</td><td>CaCl2</td><td>Calbiochem</td><td>208291-250GM</td></tr><tr><td>Collagen type I, rat-tail</td><td></td><td>Corning</td><td>354236</td></tr><tr><td>Collagen type IV, human placenta</td><td></td><td>Merck Life Science</td><td>C5533-5MG</td></tr><tr><td>Endothelial Cell Growth Medium</td><td>ECG medium</td><td>Promocell</td><td>C-22111</td></tr><tr><td>Expoxy-coated round tip tweezer</td><td>fine tweezer</td><td>Rubis Pinzette</td><td>E78144-2A</td></tr><tr><td>Fluorescent marker, dark red</td><td></td><td>Invitrogen</td><td>F8807</td></tr><tr><td>Glass coverslips, &Oslash;13 mm, #1</td><td></td><td>Epredia</td><td>CB00130RA120MNZ0</td></tr><tr><td>Glass coverslips, &Oslash;13 mm, #1.5</td><td></td><td>Epredia</td><td>CB00120RAC20MNZ0</td></tr><tr><td>Hydrochloride acid, 25%</td><td>HCl</td><td>Merck</td><td>1.100316.1000</td></tr><tr><td>Krebs-Henseleit buffer</td><td></td><td>Sigma-Aldrich</td><td>K3753</td></tr><tr><td>Microscope, Leica Application Suite X software, version 3.5.7.23225</td><td>Leica Microsystems</td><td></td><td></td></tr><tr><td>Microscope, Leica DMi8 epifluorescent microscope</td><td></td><td>Leica Microsystems</td><td></td></tr><tr><td>Microscope, Nikon Ti2 Eclipse</td><td></td><td>Nikon</td><td></td></tr><tr><td>Microscope, NIS-Elements AR software</td><td></td><td>Nikon</td><td></td></tr><tr><td>N,N,N',N'-tetramethylethane-1,2-diamine</td><td>TEMED</td><td>Merck Life Science</td><td>110-18-9</td></tr><tr><td>Nalgene bottle</td><td></td><td>Thermo Scientific</td><td>2187-0016</td></tr><tr><td>Needle, 21Gx1"</td><td></td><td>Henke Sass Wolf</td><td>HK4710008025</td></tr><tr><td>Normal serum, goat</td><td></td><td>Gibco</td><td>10098792</td></tr><tr><td>Papaverine hydrochloride</td><td></td><td>Sigma</td><td>61-25-6</td></tr><tr><td>Penicillin/Streptomyocin (10 000 U/mL)</td><td>P/S</td><td>Gibco</td><td>15140163</td></tr><tr><td>Petri-dish, large (145x20mm)</td><td></td><td>Greiner Bio-one</td><td>639160</td></tr><tr><td>Petri-dish, small (60x15mm)</td><td></td><td>Greiner Bio-one</td><td>628160</td></tr><tr><td>Phosphate Buffered Saline</td><td>PBS</td><td>Sigma</td><td>P4417</td></tr><tr><td>Pluronic F-127</td><td></td><td>Merck Life Science</td><td>P2443-250G</td></tr><tr><td>Puncture needle, sharp closed tip</td><td></td><td>unknown</td><td></td></tr><tr><td>Scalpel, no. 4</td><td></td><td>Swann-Morton</td><td></td></tr><tr><td>Sodium hydrogen carbonate</td><td>NaHCO3</td><td>VWR International</td><td>144-55-8</td></tr><tr><td>sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate</td><td>Sulfo-SANPAH</td><td>Thermo Scientific</td><td>22589</td></tr><tr><td>Surgical blade, no. 20</td><td></td><td>Swann-Morton</td><td></td></tr><tr><td>Surgical drape sheet</td><td></td><td>Foliodrape</td><td>2775001</td></tr><tr><td>Surgical tweezer</td><td></td><td>Lettix</td><td>400024</td></tr><tr><td>Triton X-100</td><td></td><td>Merck</td><td>9036-19-5</td></tr><tr><td>UV lamp</td><td></td><td>Analytik Jena</td><td>95-0042-13&nbsp;</td></tr><tr><td>well plate, 96-well, F-bottom</td><td></td><td>Greiner Bio-one</td><td>655180</td></tr><tr><td>well plate, glass bottom 12-well</td><td></td><td>MatTek</td><td>P12G-0-14-F</td></tr></tbody></table>

2.5d-model voor ex vivo mechanische karakterisering van kiemende angiogenese in levend weefsel

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

2.5D-model voor ex vivo mechanische karakterisering van kiemende angiogenese in levend weefsel

Sprouting angiogenesis is the formation of new blood vessels from pre-existing vasculature and is of great importance for physiological such as tissue ...

25d-model-for-ex-vivo-mechanical-characterization-sprouting

Research

JoVE Journal

Biology

Biologie

Deze video toont het protocol van een in vitro angiogenese test die recapituleert verschillende stadia van angiogenese. Time-lapse beelden van kiemen, lumen vorming, vertakking en anastomose - belangrijkste kenmerken van angiogenese - worden weergegeven.

Geoptimaliseerde fibrinegel Bead Assay voor de Studie van Angiogenese

De neonatale muizen netvlies biedt een goed gekarakteriseerd fysiologisch model van angiogenese, die onderzoeken van de rol van de verschillende genen of medicijnen die angiogenese moduleren in een...

Hele Mount immunofluorescentiekleuring van de Neonatale Mouse Retina naar Angiogenese Onderzoek<em&gt; In vivo</em

Vascularisatie is de sleutel tot een succesvolle aanpak in de tissue engineering. Daarom zijn betrouwbare technologieën die voor de ontwikkeling van vasculaire netwerken in weefsel-constructen evalueren. Hier presenteren we een eenvoudige en kosteneffectieve manier te visualiseren en kwantificeren vascularisatie in vivo.

Een Full Skin Defect model te evalueren Vascularisatie Biomaterialen<em&gt; In Vivo</em

We beschrijven een matrigel-plugtest om het angiogeen potentieel van een pool van factoren te illustreren die worden afgescheiden door kankercellen, met behulp van twee complementaire beeldvormingsmodaliteiten, echografie en endomicroscopie. De matrigel, een buitencelmatrix (ECM)-nabootsende gel, wordt gebruikt om de gastheer (muis) te introduceren met angiogeen factoren die worden afgescheiden naar het geconditioneerde medium...

Monitoring Functionaliteit en morfologie van vaatstelsel aangeworven door gesekreteerd door Snelgroeiende Tumor genererende cellen

We beschrijven een microchirurgische benadering voor het genereren van een arterioveneuze (AV) -lus als model voor het analyseren van vascularisatie in vivo in een geïsoleerd en goed gekarakteriseerd omgeving. Dit model is niet alleen nuttig voor het onderzoek naar angiogenese, maar ook optimaal geschikt voor engineering axiaal gevasculariseerd en transplantable weefsels.

De arterioveneuze (AV) Loop in een klein proefdiermodel voor Angiogenese en Gevasculariseerd Tissue Engineering Studie

Deze methode beschrijft de cultuur van door IPSC afkomstige endotheliale cellen als 40 geperfundeerd 3D Micro vaten in een gestandaardiseerd microfluïdisch platform. Dit platform maakt de studie mogelijk van gradiënt-gedreven angiogene kiemen in 3D, inclusief anastomose en stabilisatie van de angiogene spruiten op een schaalbare en hoge doorvoer wijze.

Gestandaardiseerde en schaalbare assay voor het bestuderen van Perfused 3D-angiogene kiemen van door iPSC afgeleide endotheliale cellen in vitro

Driedimensionaal co-Culture spheroid-systeem voor angiogenese is ontworpen om de fysiologische angiogenese na te bootsen. Co-Culture sferoïden worden gevormd door twee humane vasculaire celprecursoren, ecfcs en MSCS, en ingebed in collageen gel. Het nieuwe systeem is effectief voor het evalueren van angiogene modulatoren en verschaft relevantere informatie aan de in vivo studie.

Driedimensionaal angiogenese-assay systeem met co-Culture Spheroids gevormd door endotheliale kolonie vormende cellen en mesenchymale stamcellen

Hier bieden we een microfluïdische chip en een automatisch gecontroleerd, zeer efficiënt circulatiemicrofluïdisch systeem dat de initiële micromilieu van neovascularisatie samenvat, waardoor endotheelcellen (EC's) tegelijkertijd kunnen worden gestimuleerd door hoge luminale schuifspanning, fysiologisch niveau van transendotheliale stroom en verschillende vasculaire endotheliale groeifactor (VEGF) distributie tegelijkertijd.

Microfluïdisch model om de eerste gebeurtenis van neovascularisatie na te bootsen

Deze test maakt gebruik van embryonale stamcellen van muizen gedifferentieerd in embryoïde lichamen gekweekt in 3D-collageengel om de biologische processen te analyseren die kiemende angiogenese in vitro beheersen. De techniek kan worden toegepast voor het testen van medicijnen, het modelleren van ziekten en voor het bestuderen van specifieke genen in de context van deleties die embryonaal dodelijk zijn.

In vitro Driedimensionale kiemtest van angiogenese met behulp van embryonale stamcellen van muizen voor vaatziektemodellering en drugstests

Deze studie presenteert de tweedimensionale (2D) kraswondmigratietest en de driedimensionale (3D) sferoïde kiemtest, samen met hun respectievelijke stroomafwaartse analysemethoden, waaronder RNA-extractie en immunocytochemie, als geschikte tests om angiogenese in vitro te bestuderen.

Onderzoek naar angiogenese op functioneel en moleculair niveau door gebruik te maken van de Scratch Wound Migration Assay en de Spheroid Sprouting Assay

Verken een 2.5D ex vivo model voor mechanische karakterisering van angiogenese in varkenscarotis. Deze methode combineert tractiekrachtmicroscopie met fysiologisch relevant weefselonderzoek.

2.5D-model voor ex vivo mechanische karakterisering van kiemende angiogenese in levend weefsel

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