Name: Heteromulticellulaire stromale cellen in steigervrije 3D-culturen van epitheelkankercellen om invasie te stimuleren (Video) | JoVE Journal
Uploaded: 2025-04-04T14:20:00.000+00:00
Duration: 9 min 18 s
Description: Ontdek een kosteneffectieve methode voor 3D-celculturen met en zonder steiger om heterocellulaire interacties in epitheelkanker te bestuderen. Dit protocol helpt onderzoekers bij het begrijpen van metastase en therapieresistentie.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>de Visser, K. E., Joyce, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+evolving+tumor+microenvironment%3A+From+cancer+initiation+to+metastatic+outgrowth%5BTitle%5D)+AND+%22Cancer+Cell%22%5BJournal%5D)">The evolving tumor microenvironment: From cancer initiation to metastatic outgrowth.</a> Cancer Cell. 41 (3), 374-403 (2023).</li>
<li>Hanahan, D., Weinberg, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hallmarks+of+cancer%3A+The+next+generation%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Hallmarks of cancer: The next generation.</a> Cell. 144 (5), 646-674 (2011).</li>
<li>Anderson, N. M., Simon, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+tumor+microenvironment%5BTitle%5D)+AND+%22Curr+Biol%22%5BJournal%5D)">The tumor microenvironment.</a> Curr Biol. 30 (16), R921-R925 (2020).</li>
<li>Baghban, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Tumor+microenvironment+complexity+and+therapeutic+implications+at+a+glance%5BTitle%5D)+AND+%22Cell+Commun+Signal%22%5BJournal%5D)">Tumor microenvironment complexity and therapeutic implications at a glance.</a> Cell Commun Signal. 18 (1), 59(2020).</li>
<li>Sahai, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+framework+for+advancing+our+understanding+of+cancer-associated+fibroblasts%5BTitle%5D)+AND+%22Nat+Rev+Cancer%22%5BJournal%5D)">A framework for advancing our understanding of cancer-associated fibroblasts.</a> Nat Rev Cancer. 20 (3), 174-186 (2020).</li>
<li>Erdogan, B., Webb, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cancer-associated+fibroblasts+modulate+growth+factor+signaling+and+extracellular+matrix+remodeling+to+regulate+tumor+metastasis%5BTitle%5D)+AND+%22Biochem+Soc+Trans%22%5BJournal%5D)">Cancer-associated fibroblasts modulate growth factor signaling and extracellular matrix remodeling to regulate tumor metastasis.</a> Biochem Soc Trans. 45 (1), 229-236 (2017).</li>
<li>Iozzo, R. V., Sanderson, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Proteoglycans+in+cancer+biology%2C+tumour+microenvironment+and+angiogenesis%5BTitle%5D)+AND+%22J+Cell+Mol+Med%22%5BJournal%5D)">Proteoglycans in cancer biology, tumour microenvironment and angiogenesis.</a> J Cell Mol Med. 15 (5), 1013-1031 (2011).</li>
<li>Amersfoort, J., Eelen, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immunomodulation+by+endothelial+cells+-+partnering+up+with+the+immune+system%5BTitle%5D)+AND+%22Nat+Rev+Immunol%22%5BJournal%5D)">Immunomodulation by endothelial cells - partnering up with the immune system.</a> Nat Rev Immunol. 22 (9), 576-588 (2022).</li>
<li>Balani, S., Nguyen, L. V., Eaves, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Modeling+the+process+of+human+tumorigenesis%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">Modeling the process of human tumorigenesis.</a> Nat Commun. 8 (1), 15422(2017).</li>
<li>Drost, J., Clevers, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Organoids+in+cancer+research%5BTitle%5D)+AND+%22Nat+Rev+Cancer%22%5BJournal%5D)">Organoids in cancer research.</a> Nat Rev Cancer. 18 (7), 407-418 (2018).</li>
<li>Wang, E., Xiang, K., Zhang, Y., Wang, X. -F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Patient-derived+organoids+%28PDOs%29+and+PDO-derived+xenografts+%28PDOXs%29%3A+New+opportunities+in+establishing+faithful+pre-clinical+cancer+models%5BTitle%5D)+AND+%22J+Natl+Cancer+Cent%22%5BJournal%5D)">Patient-derived organoids (PDOs) and PDO-derived xenografts (PDOXs): New opportunities in establishing faithful pre-clinical cancer models.</a> J Natl Cancer Cent. 2 (4), 263-276 (2022).</li>
<li>Kamb, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((What%27s+wrong+with+our+cancer+models%5BTitle%5D)+AND+%22Nat+Rev+Drug+Discov%22%5BJournal%5D)">What's wrong with our cancer models.</a> Nat Rev Drug Discov. 4 (2), 161-165 (2005).</li>
<li>Tuveson, D., Clevers, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cancer+modeling+meets+human+organoid+technology%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Cancer modeling meets human organoid technology.</a> Science. 364 (6444), 952-955 (2019).</li>
<li>Zitvogel, L., Pitt, J. M., Daillère, R., Smyth, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mouse+models+in+oncoimmunology%5BTitle%5D)+AND+%22Nat+Rev+Cancer%22%5BJournal%5D)">Mouse models in oncoimmunology.</a> Nat Rev Cancer. 16 (12), 759-773 (2016).</li>
<li>Liu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Patient-derived+xenograft+models+in+cancer+therapy%3A+technologies+and+applications%5BTitle%5D)+AND+%22Signal+Transduct+Target+Ther%22%5BJournal%5D)">Patient-derived xenograft models in cancer therapy: technologies and applications.</a> Signal Transduct Target Ther. 8 (1), 160(2023).</li>
<li>Ben-David, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Patient-derived+xenografts+undergo+mouse-specific+tumor+evolution%5BTitle%5D)+AND+%22Nat+Genet%22%5BJournal%5D)">Patient-derived xenografts undergo mouse-specific tumor evolution.</a> Nat Genet. 49 (11), 1567-1575 (2017).</li>
<li>Byrne, A. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Interrogating+open+issues+in+cancer+precision+medicine+with+patient-derived+xenografts%5BTitle%5D)+AND+%22Nat+Rev+Cancer%22%5BJournal%5D)">Interrogating open issues in cancer precision medicine with patient-derived xenografts.</a> Nat Rev Cancer. 17 (4), 254-268 (2017).</li>
<li>Ivanics, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Patient-derived+xenograft+cryopreservation+and+reanimation+outcomes+are+dependent+on+cryoprotectant+type%5BTitle%5D)+AND+%22Lab+Invest%22%5BJournal%5D)">Patient-derived xenograft cryopreservation and reanimation outcomes are dependent on cryoprotectant type.</a> Lab Invest. 98 (7), 947-956 (2018).</li>
<li>Biju, T. S., Priya, V. V., Francis, A. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Role+of+three-dimensional+cell+culture+in+therapeutics+and+diagnostics%3A+an+updated+review%5BTitle%5D)+AND+%22Drug+Deliv+and+Transl+Res%22%5BJournal%5D)">Role of three-dimensional cell culture in therapeutics and diagnostics: an updated review.</a> Drug Deliv and Transl Res. 13 (9), 2239-2253 (2023).</li>
<li>Urzì, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+cell+cultures%3A+The+bridge+between+in+vitro+and+in+vivo+models%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Three-dimensional cell cultures: The bridge between in vitro and in vivo models.</a> Int J Mol Sci. 24 (15), 12046(2023).</li>
<li>Cacciamali, A., Villa, R., Dotti, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+cell+cultures%3A+Evolution+of+an+ancient+tool+for+new+applications%5BTitle%5D)+AND+%22Front+Physiol%22%5BJournal%5D)">3D cell cultures: Evolution of an ancient tool for new applications.</a> Front Physiol. 13, 836480(2022).</li>
<li>Abuwatfa, W. H., Pitt, W. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Scaffold-based+3D+cell+culture+models+in+cancer+research%5BTitle%5D)+AND+%22J+Biomed+Sci%22%5BJournal%5D)">Scaffold-based 3D cell culture models in cancer research.</a> J Biomed Sci. 31 (1), 7(2024).</li>
<li>Yakavets, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advanced+co-culture+3D+breast+cancer+model+for+investigation+of+fibrosis+induced+by+external+stimuli%3A+optimization+study%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Advanced co-culture 3D breast cancer model for investigation of fibrosis induced by external stimuli: optimization study.</a> Sci Rep. 10 (1), 21273(2020).</li>
<li>Mohammadrezaei, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Predicting+and+elucidating+the+post-printing+behavior+of+3D+printed+cancer+cells+in+hydrogel+structures+by+integrating+in-vitro+and+in-silico+experiments%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Predicting and elucidating the post-printing behavior of 3D printed cancer cells in hydrogel structures by integrating in-vitro and in-silico experiments.</a> Sci Rep. 13 (1), 1211(2023).</li>
<li>Kuen, J., Darowski, D., Kluge, T., Majety, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pancreatic+cancer+cell%2Ffibroblast+co-culture+induces+M2+like+macrophages+that+influence+therapeutic+response+in+a+3D+model%5BTitle%5D)+AND+%22PLOS+ONE%22%5BJournal%5D)">Pancreatic cancer cell/fibroblast co-culture induces M2 like macrophages that influence therapeutic response in a 3D model.</a> PLOS ONE. 12 (7), e0182039(2017).</li>
<li>Bray, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Global+cancer+statistics+2022%3A+GLOBOCAN+estimates+of+incidence+and+mortality+worldwide+for+36+cancers+in+185+countries%5BTitle%5D)+AND+%22CA+Cancer+J+Clin%22%5BJournal%5D)">Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.</a> CA Cancer J Clin. 74 (3), 229-263 (2024).</li>
<li>Kennecke, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metastatic+behavior+of+breast+cancer+subtypes%5BTitle%5D)+AND+%22J+Clin+Oncol%22%5BJournal%5D)">Metastatic behavior of breast cancer subtypes.</a> J Clin Oncol. 28 (20), 3271-3277 (2010).</li>
<li>Neve, R. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+collection+of+breast+cancer+cell+lines+for+the+study+of+functionally+distinct+cancer+subtypes%5BTitle%5D)+AND+%22Cancer+Cell%22%5BJournal%5D)">A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes.</a> Cancer Cell. 10 (6), 515-527 (2006).</li>
<li>Pingitore, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+multilineage+3D+spheroids+as+a+model+of+liver+steatosis+and+fibrosis%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Human multilineage 3D spheroids as a model of liver steatosis and fibrosis.</a> Int J Mol Sci. 20 (7), 1629(2019).</li>
<li>Bar-Ephraim, Y. E., Kretzschmar, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Organoids+in+immunological+research%5BTitle%5D)+AND+%22Nat+Rev+Immunol%22%5BJournal%5D)">Organoids in immunological research.</a> Nat Rev Immunol. 20 (5), 279-293 (2020).</li>
<li>Beerkens, A. P. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterizing+OXPHOS+inhibitor-mediated+alleviation+of+hypoxia+using+high-throughput+live+cell-imaging%5BTitle%5D)+AND+%22Cancer+Metab%22%5BJournal%5D)">Characterizing OXPHOS inhibitor-mediated alleviation of hypoxia using high-throughput live cell-imaging.</a> Cancer Metab. 12 (1), 13(2024).</li>
<li>Feaster, T. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Matrigel+mattress%5BTitle%5D)+AND+%22Cir+Res%22%5BJournal%5D)">Matrigel mattress.</a> Cir Res. 117 (12), 995-1000 (2015).</li>
<li>Huang, Z., Yu, P., Tang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+triple-negative+breast+cancer+MDA-MB-231+cell+spheroid+model%5BTitle%5D)+AND+%22Onco+Targets+Ther%22%5BJournal%5D)">Characterization of triple-negative breast cancer MDA-MB-231 cell spheroid model.</a> Onco Targets Ther. 13, 5395-5405 (2020).</li>
<li>Nam, S., Khawar, I. A., Park, J. K., Chang, S., Kuh, H. -J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cellular+context-dependent+interaction+between+cancer+and+stellate+cells+in+hetero-type+multicellular+spheroids+of+pancreatic+tumor%5BTitle%5D)+AND+%22Biochem+Biophys+Res+Commun%22%5BJournal%5D)">Cellular context-dependent interaction between cancer and stellate cells in hetero-type multicellular spheroids of pancreatic tumor.</a> Biochem Biophys Res Commun. 515 (1), 183-189 (2019).</li>
<li>Monteiro, M. V., Gaspar, V. M., Ferreira, L. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hydrogel+3D+in+vitro+tumor+models+for+screening+cell+aggregation+mediated+drug+response%5BTitle%5D)+AND+%22Biomater+Sci%22%5BJournal%5D)">Hydrogel 3D in vitro tumor models for screening cell aggregation mediated drug response.</a> Biomater Sci. 8 (7), 1855-1864 (2020).</li>
</ol>

<table><tbody><tr><td>15 mL Polypropylene Centrifuge Tubes&nbsp;&nbsp;</td><td>Falcon&nbsp;</td><td>14-959-53A&nbsp;</td><td></td></tr><tr><td>96-Well, Treated, U-Shaped-Bottom Microplate&nbsp;</td><td>Thermo Scientific&nbsp;</td><td>12566432&nbsp;</td><td></td></tr><tr><td>100 mm Dish Cell Culture Treated Surface&nbsp;</td><td>Thermo Scientific&nbsp;</td><td>130182&nbsp;</td><td></td></tr><tr><td>170 L CO2 Incubators&nbsp;</td><td>VWR</td><td>VWR51014991(10810902)</td><td></td></tr><tr><td>200 Self-Sealing Barrier Pipet Tips</td><td>Fisher Scientific</td><td>02-682-255</td><td></td></tr><tr><td>500 mL DMEM/Hams F-12, [+] L-glutamine</td><td>Corning</td><td>10-090-CV</td><td></td></tr><tr><td>1000 Self-Sealing Barrier Pipet Tips</td><td>Molecular Biology Products Inc.</td><td>2179HR</td><td></td></tr><tr><td>Animal-Free Recombinant Human EGF</td><td>PeproTech</td><td>AF-100-15</td><td></td></tr><tr><td>Automated Pipette Repeater E3x</td><td>Eppendorf</td><td>4987000410</td><td></td></tr><tr><td>Basement membrane solution</td><td>Huntsman Cancer Institute&nbsp;</td><td>https://healthcare.utah.edu/huntsmancancerinstitute/</td><td></td></tr><tr><td>Bead Bath</td><td>Lab Armor&nbsp;</td><td>74300706</td><td></td></tr><tr><td>Biorender&nbsp;</td><td>Biorender</td><td>Biorender.com&nbsp;</td><td></td></tr><tr><td>Biosafety Laminar Flow Cabinets&nbsp;</td><td>Thermo Scientific</td><td>300590389</td><td></td></tr><tr><td>BioSpa 8 Automated Incubator&nbsp;</td><td>Agilent&nbsp;&nbsp;</td><td>23082120</td><td></td></tr><tr><td>Cell Counter&nbsp;</td><td>Invitrogen</td><td>AMQAF2000</td><td></td></tr><tr><td>Cell Counting Chamber Slides</td><td>Thermo Scientific</td><td>C10283</td><td></td></tr><tr><td>CellTracker Deep Red&nbsp;</td><td>Thermo Fisher Scientific&nbsp;</td><td>C34565&nbsp;</td><td></td></tr><tr><td>Centrifuge&nbsp;</td><td>Thermo Scientific&nbsp;</td><td>13100675</td><td></td></tr><tr><td>Cholera Toxin B subunit</td><td>Sigma-Aldrich</td><td>C9903-1MG</td><td></td></tr><tr><td>Combitips advanced, Sterile, 5.0 mL</td><td>Fisher Scientific</td><td>13-683-714</td><td></td></tr><tr><td>Cytation 10&nbsp;Reader Imager</td><td>Agilent&nbsp;</td><td>2105245</td><td></td></tr><tr><td>Defined Trypsin Inhibitor&nbsp;</td><td>Gibco&nbsp;</td><td>R007100&nbsp;</td><td>soybean trypsin inhibitor in 1x PBS</td></tr><tr><td>Donor Horse Serum, 500 mL, United States Origin</td><td>Corning</td><td>35-030-CV</td><td></td></tr><tr><td>Dulbecco's Modified Eagle's Medium&nbsp;</td><td>Corning&nbsp;</td><td>10-013-CV&nbsp;</td><td></td></tr><tr><td>Excel Sofware&nbsp;</td><td>Microsoft office 365&nbsp;</td><td>https://www.microsoft.com/en-us/microsoft-365/excel</td><td></td></tr><tr><td>Fetal Bovine Serum&nbsp;</td><td>Thermo Scientific&nbsp;</td><td>26140079&nbsp;</td><td></td></tr><tr><td>Fetal Bovine Serum, qualified, US origin</td><td>Thermo Scientific</td><td>26140079</td><td></td></tr><tr><td>Gentamicin Sulfate, Liquid</td><td>Corning</td><td>30-005-CR</td><td></td></tr><tr><td>GraphPad Prism</td><td></td><td></td><td></td></tr><tr><td>Hydrocortisone solution,50 &micro;M, sterile-filtered, BioXtra</td><td>Sigma-Aldrich</td><td>H6909-10ML</td><td></td></tr><tr><td>Image J Software&nbsp;</td><td>Fiji&nbsp;</td><td>https://imagej.net/ij/</td><td></td></tr><tr><td>Insulin solution human</td><td>Sigma-Aldrich</td><td>I9278-5ML</td><td></td></tr><tr><td>Magnifying Glass&nbsp;</td><td>Fisher Scientific&nbsp;</td><td>01-182-392&nbsp;</td><td></td></tr><tr><td>Microcentrifuge Tube&nbsp;</td><td>Costar&nbsp;</td><td>3621&nbsp;</td><td></td></tr><tr><td>Microscope</td><td>Olympus</td><td>CKX53SF</td><td></td></tr><tr><td>Mini Lightbox&nbsp;</td><td>Fisher Scientific&nbsp;</td><td>361044708&nbsp;</td><td></td></tr><tr><td>Molecular Probes CellTracker Blue CMAC Dye&nbsp;</td><td>Fisher Scientific&nbsp;</td><td>C2110&nbsp;</td><td></td></tr><tr><td>Molecular Probes CellTracker Orange CMRA Dye&nbsp;</td><td>Fisher Scientific&nbsp;</td><td>C34551&nbsp;</td><td></td></tr><tr><td>Penicillin-Streptomycin Solution</td><td>Cytiva</td><td>SV30010</td><td></td></tr><tr><td>Penicillin-Streptomycin Solution&nbsp;</td><td>Cytiva&nbsp;</td><td>SV30010&nbsp;</td><td></td></tr><tr><td>Phase-Contrast Microscope&nbsp;</td><td>Echo&nbsp;</td><td>RVSF1000</td><td></td></tr><tr><td>Phosphate Buffered Saline&nbsp;</td><td>Fisher Scientific&nbsp;</td><td>SH3025601&nbsp;</td><td></td></tr><tr><td>Pipet Controller</td><td>Corning</td><td>4099</td><td></td></tr><tr><td>Pipette 100 &ndash; 1,000 &micro;L</td><td>Eppendorf</td><td>3123000063</td><td></td></tr><tr><td>Pipette 20 &ndash; 200 &micro;L</td><td>Eppendorf</td><td>3123000055</td><td></td></tr><tr><td>Prism&nbsp;</td><td>GraphPad Software</td><td>https://www.graphpad.com/features</td><td></td></tr><tr><td>RPMI 1640 with L-glutamine and 25 mM HEPES</td><td>Corning</td><td>10-041-CV</td><td></td></tr><tr><td>Serological Pipette, 10 mL</td><td>Thermo Scientific</td><td>170356N</td><td></td></tr><tr><td>Serological Pipette, 25 mL</td><td>Thermo Scientific</td><td>170357N</td><td></td></tr><tr><td>Serological Pipette, 5 mL</td><td>Thermo Scientific</td><td>170355N</td><td></td></tr><tr><td>Trypan Blue Solution, 0.4%&nbsp;</td><td>Corning&nbsp;</td><td>25900CI&nbsp;</td><td></td></tr><tr><td>Trypsin-EDTA, 0.25%&nbsp;</td><td>Corning&nbsp;</td><td>25053CI&nbsp;</td><td></td></tr></tbody></table>

heteromulticellulaire stromale cellen in steigervrije 3d-culturen van epitheelkankercellen om invasie te stimuleren

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Heteromulticellulaire stromale cellen in steigervrije 3D-culturen van epitheelkankercellen om invasie te stimuleren

Breast cancer is the second leading cause of cancer-related death among women in the U.S. Organoid models of solid tumors have been shown to faithfully ...

heteromulticellular-stromal-cells-scaffold-free-3d-cultures

Research

JoVE Journal

Cancer Research

Kankeronderzoek

Een eenvoudige methode beschreven voor het analyseren effecten weefsel fibroblasten op geassocieerde epitheelcellen. De combinatie van deze methode en driedimensionale weefselkweek kan worden vergemakkelijkt analyse van cellen na isolatie van 3D. De techniek is voor cellen van verschillende maligne potentieel, waardoor systematisch onderzoek effecten van tumor-geassocieerde stroma op tumorcellen.

Isolatie van Borst epitheelcellen van drie-dimensionale Mixed-cel Spheroid Co-cultuur

Heterotypische Driedimensionale<em&gt; In Vitro</em&gt; Modellering van Stroma-epitheliale interacties tijdens eierstokkanker initiatie en progressie

This video describes the technique of utilizing a native matrix to study tumor cell invasion. The technique used in this assay provides the ability to assess epithelial-mesenchymal cell interactions in a 3D setting without the need for a synthetic or foreign matrix.

Tumor Cell Invasion Assay: A 3D In Vitro Native Matrix-based Method to Assess Epithelial-mesenchymal Cell Interactions

Weefselbewerkt-fibroblast afgeleid inheemse matrix is ​​een opkomende hulpmiddel om een ​​stromale substraat dat epitheelcelproliferatie en differentiatie ondersteunt genereren. Hier een protocol deze methode toegepast op de impact van verschillende stromale celtypen op tumorcel biologie beoordelen wordt gepresenteerd.

Tissue Engineering van Tumor Stromal Microenvironment met Toepassing op Cancer Cell Invasion

Dit artikel geeft gedetailleerde methodologieën voor het gebruik van driedimensionale (3D) assays om borstkanker cel invasie kwantificeren. Concreet bespreken we het nodig om dergelijke assays procedures, kwantificering en gegevensanalyse, alsook werkwijzen voor het verlies van membraan integriteit die ontstaat wanneer cellen binnendringen onderzoeken.

Kwantificering van de Breast Cancer Cell Invasiviteit met een drie-dimensionale (3D) Model

Hier presenteren we een protocol voor co-cultuur in drie dimensies, wat nuttig is voor het onderzoeken van interacties tussen meerdere cellen en het beïnvloeden van het gedrag van kankercellen door de extracellulaire matrix. In dit experimentele model worden kankercellen gekweekt op collageengels ingebed met humane kanker-geassocieerde fibroblasten.

Driedimensionale Co-cultuur Model voor Tumor-stromale Interactie

Deze methode evalueert de invasie van kankercellen uit sferoïden in een omringende 3D-matrix.  Sferoïden worden gegenereerd via de hanging drop-kweekmethode en vervolgens ingebed in een matrix bestaande uit basement membraanmaterialen en type I collageen.  Invasie uit de sferoïden wordt vervolgens gemonitord.

Een Cancer Cell Sferoïde Assay om Invasion beoordelen in een 3D-instelling

Kankercellen worden ingebed in een collageengel en vervolgens tussen een acellulair fibrinegel gedaan om een 3D-kweeksysteem te genereren waarin de invasiviteit en vorming van satelliettumoren kunnen worden gemonitord.

Driedimensionale Culture Assay te ontdekken Cancer Cell Invasiviteit en Satellite tumorvorming

Een nieuwe drie-dimensionale sferoïde model gebaseerd op de heterotypische interactie van tumorcellen en stromal fibroblasten wordt vastgesteld. Hier presenteren we cocultuur van tumorcellen en stromalfibroblasten, time-lapse beeldvorming en confocale microscopie om de vorming van sferoïden te visualiseren. Dit driedimensionale model biedt een relevant platform om tumor-stroma interacties te bestuderen en kankertherapieën te testen.

Een nieuwe Stromal Fibroblast-Gemoduleerde 3D Tumor Sferoïde Model voor het bestuderen van Tumor-Stroma Interactie en Drug Discovery

De gepresenteerde aanpak evalueert tegelijkertijd kankercelinvasie in 3D-sferoïde testen en T-celcytotoxiciteit. Sferoïden worden gegenereerd in een steiger-vrije agarose multi-microwell gegoten. Co-cultuur en inbedding in type I collageen matrix worden uitgevoerd in hetzelfde apparaat dat het mogelijk maakt om kankercel invasie en T-cel gemedited cytotoxiciteit te controleren.

Monitoring Cancer Cell Invasion en T-Cell Cytotoxicity in 3D Cultuur

Invasie is een belangrijk biologisch fenomeen bij de ontwikkeling en progressie van kanker. Dit proces wordt beïnvloed door niet-neoplastische cellen en componenten van de micro-omgeving van de tumor. Het doel van deze studie is het beschrijven van een alternatieve methode voor het analyseren van tumorcelinvasie in vitro met behulp van driedimensionale (3D) culturen.

Alternatieve strategie om in vitro celinvasie van 3D-culturen te analyseren

Ontdek een kosteneffectieve methode voor 3D-celculturen met en zonder steiger om heterocellulaire interacties in epitheelkanker te bestuderen. Dit protocol helpt onderzoekers bij het begrijpen van metastase en therapieresistentie.

Heteromulticellulaire stromale cellen in steigervrije 3D-culturen van epitheelkankercellen om invasie te stimuleren

Meer video's verkennen