Name: Chronische craniale venstertechniek voor herhaalde corticale opnames tijdens anesthesie bij varkens (Video) | JoVE Journal
Uploaded: 2025-06-06T13:45:00.000+00:00
Duration: 7 min 19 s
Description: Leer een chronische craniale venstertechniek voor herhaalde corticale opnames bij varkens. Deze methode verbetert signaalintegriteit en vermindert risico's van implantaatfalen in pijnonderzoek.

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<table><tbody><tr><td>Cranial window implantation</td><td></td><td></td><td></td></tr><tr><td>Scalpel</td><td></td><td></td><td></td></tr><tr><td>Tweezers</td><td></td><td></td><td></td></tr><tr><td>Gauze</td><td></td><td></td><td></td></tr><tr><td>Cauterizer</td><td></td><td></td><td></td></tr><tr><td>Periosteum elevator</td><td></td><td></td><td>plat, 10 mm breedte</td></tr><tr><td>Weitlaner retractor</td><td></td><td></td><td>3x4 punten, stomp, 16,5 cm</td></tr><tr><td>Midas Rex Legend EHS stylus chirurgisch boorsysteem</td><td>Medtronic Powered Surgical Solutions, Fort Worth, VS</td><td></td><td></td></tr><tr><td>Legend Ball Fine boortje&nbsp;</td><td>Medtronic Powered Surgical Solutions, Fort Worth, VS</td><td>7BA40F-MN en 7BA60F-MN</td><td>MedNext type 4 en 6 mm kopdiameter</td></tr><tr><td>Steriele afdekking voor de boor</td><td></td><td></td><td></td></tr><tr><td>Spuit</td><td></td><td></td><td>5 ml</td></tr><tr><td>Zoutoplossing</td><td></td><td></td><td></td></tr><tr><td>Zuigpomp</td><td></td><td></td><td></td></tr><tr><td>Liniaal</td><td></td><td></td><td></td></tr><tr><td>Wattenstaafjes</td><td></td><td></td><td></td></tr><tr><td>Rongeur</td><td></td><td></td><td>rechte kaken, 15 cm, 3 x 20 mm bitgrootte</td></tr><tr><td>2,5 mm handboor en handgreep</td><td></td><td></td><td></td></tr><tr><td>Butterfly infuusset</td><td></td><td></td><td></td></tr><tr><td>Zes M3 schroeven in 6-14 mm lengte</td><td></td><td></td><td></td></tr><tr><td>Schroevendraaier&nbsp;</td><td></td><td></td><td></td></tr><tr><td>Drie maten 3D-geprinte craniale venster (4-, 5- en 6-mm diepte) en dop</td><td></td><td></td><td></td></tr><tr><td>Deisoleerd U-vormige connector</td><td></td><td></td><td></td></tr><tr><td>Krokodillenconnector</td><td></td><td></td><td></td></tr><tr><td>Genta-coll resorbeerbare antibioticumspons</td><td></td><td></td><td></td></tr><tr><td>VYCRIL resorbeerbare hechting&nbsp;</td><td>Ethicon</td><td></td><td>2-0 26 mm ronde lichaam</td></tr><tr><td>Monocryl niet-resorbeerbare hechting</td><td>Ethicon</td><td></td><td>3-0 24 mm omgekeerde snij</td></tr><tr><td>Naaldhaak</td><td></td><td></td><td></td></tr><tr><td>Schaar</td><td></td><td></td><td></td></tr><tr><td>Topisch zelfklevend weefsellijm</td><td>Leukosan</td><td></td><td></td></tr><tr><td>Implantatie van perifere draad</td><td></td><td></td><td></td></tr><tr><td>Twee gedeeltelijk niet-geïsoleerde Cooner draden</td><td></td><td></td><td></td></tr><tr><td>OPMERKING: 1-2cm van de draden is niet geïsoleerd in het midden en aan een van de uiteinden</td><td></td><td></td><td></td></tr><tr><td>Twee 23-gauge naalden</td><td></td><td></td><td></td></tr><tr><td>Gauze</td><td></td><td></td><td></td></tr><tr><td>Programmeerbare stimulator bestuurd door een pc met MC_stimulus</td><td>Multichannel Systems, Reutlingen, Duitsland</td><td>STG4008</td><td></td></tr><tr><td>Twee krokodillenconnectoren</td><td></td><td></td><td></td></tr><tr><td>Corticale opnamen</td><td></td><td></td><td></td></tr><tr><td>Metalen plaat voor de magnetische micromanipulator&nbsp;</td><td></td><td></td><td></td></tr><tr><td>Magnetische micromanipulator stand</td><td></td><td></td><td></td></tr><tr><td>Micromanipulator</td><td></td><td></td><td></td></tr><tr><td>Headstage houder</td><td></td><td></td><td></td></tr><tr><td>32-kanaals ZIF-clip headstage</td><td>TDT, Alachua, FL, VS</td><td></td><td></td></tr><tr><td>32-kanaals micro-elektrocorticografische (&micro;ECoG) array&nbsp;</td><td>Neuronexus, Ann Arbor, VS</td><td>E32-1000-30-200</td><td></td></tr><tr><td>TDT opnameapparatuur inclusief voorversterker SI8, dataprocessor RZ2 en werkstation WS8&nbsp;</td><td>TDT, Alachua, FL, VS</td><td></td><td></td></tr><tr><td>Anesthesie &amp; Pijnbestrijding</td><td></td><td></td><td></td></tr><tr><td>Zoletil 50 Vet</td><td>Virbac, Carros, Frankrijk</td><td></td><td></td></tr><tr><td>Rompun (Zxylacin)</td><td>Elanco Animal Health Monheim; Duitsland</td><td></td><td></td></tr><tr><td>Torbugesic (Butorphanol)</td><td>Intervet International, Boxmeer, Nederland</td><td></td><td>Tegenwoordig wordt een andere formulering (dolorex) gebruikt</td></tr><tr><td>Ketaminol</td><td>Intervet International, Boxmeer, Nederland</td><td></td><td></td></tr><tr><td>Propofol</td><td>Baxter Holding, Utrecht, Nederland</td><td></td><td></td></tr><tr><td>Fentanyl</td><td>B. Braun, Melsungen, Duitsland</td><td></td><td></td></tr><tr><td>Metacam</td><td>Borhringer Ingelheim Vetmedica, Ingelheim, Duitsland</td><td></td><td></td></tr><tr><td>Noromox</td><td>Scanvet Animal Health, Fredensborg, Denemarken</td><td></td><td></td></tr><tr><td>Pentobarb</td><td>LeVet, Oudewater, Nederland</td><td></td><td></td></tr></tbody></table>

chronische craniale venstertechniek voor herhaalde corticale opnames tijdens anesthesie bij varkens

Chronic Cranial Window Technique for Repeated Cortical Recordings During Anesthesia in Pigs

Chronische craniale venstertechniek voor herhaalde corticale opnames tijdens anesthesie bij varkens

Cortical recordings are essential for extracting neuronal signals to inform various applications, including brain-computer interfaces and disease ...

chronic-cranial-window-technique-for-repeated-cortical-recordings

Research

JoVE Journal

Neuroscience

Neurowetenschap

Deze video en protocol laten zien hoe je een met glas overdekt craniale venster in knaagdieren implantaat. Deze preparaten kunnen worden gebruikt voor chronische in vivo twee-foton beeldvorming van de neocortex loop van de tijd schalen van maanden. Het kan ook worden gebruikt voor andere vormen van beeldvorming, met inbegrip van optische intrinsieke signaal beeldvorming.

Een craniotomie chirurgische ingreep voor chronische Brain Imaging

This video demonstrates the measurement of glutamate levels in the brain of an anesthetized neonatal piglet using a microelectrode array (MEA). The procedure involves creating a craniotomy window in the pig skull and inserting the MEA into the brain region of interest. The glutamate-oxidase-coated recording sites of the MEA convert extracellular brain glutamate to hydrogen peroxide, which generates a measurable current. This current is measured and normalized against background noise to...

Measuring Brain Glutamate Levels Under Anesthesia in Neonatal Piglets Using a Microelectrode Array

This video demonstrates the implantation of a soft electrocorticography array and the long-term cortical recordings in a minipig. It outlines the steps involved in surgically implanting the array over the minipig's auditory cortex and recording both baseline and sound stimulus-evoked neuronal action potentials.

Subdural Implantation of a Soft Electrocorticography Array for Cortical Electrophysiology Recording in Minipigs

Ervaring-afhankelijke moleculaire veranderingen in neuronen zijn essentieel voor het vermogen van de hersenen aan te passen als reactie op gedrags-uitdagingen.

In vivo twee-foton beeldvorming van ervaring-afhankelijke moleculaire veranderingen in corticale neuronen

Dit protocol beschrijft een eenvoudige methode voor gelijktijdige opname van co gelokaliseerde elektro-encefalografie (EEG) en multi-laminaire lokale veld potentieel in een narcose rat. Een burr gat geboord in de schedel voor het inbrengen van een micro-elektrode is aangetoond dat het te verwaarlozen vervorming van het EEG signaal veroorzaken.

Gelijktijdige opname van elektro-encefalografie mede gelokaliseerde en lokale veld potentieel in knaagdier

Deze studie verkent het nieuwe gebruik van enzym gebaseerde micro-elektrode matrix (MEA) technologie om te controleren in vivo neurotransmitter activiteit bij biggen. De hypothese was dat glutamaat disregulatie draagt bij aan het mechanisme van verdoving neurotoxiciteit. Hier presenteren we een protocol aan te passen van MEA technologie om te bestuderen van het mechanisme van anesthesie-geïnduceerde neurotoxiciteit.

Aanpassing van micro-elektrode Array technologie voor de studie van anesthesie-geïnduceerde neurotoxiciteit in de hersenen Intact Knorretje

Hier wordt een protocol gepresenteerd voor de implantatie van een chronisch schedelvenster voor de longitudinale beeldvorming van hersencellen in wakkere, hoofdbezorgde muizen.

Implantatie van een schedelvenster voor herhaalde in vivo beeldvorming bij wakkere muizen

Hier presenteren we een methode voor de prestatie- en veiligheidsbeoordeling op lange termijn van zachte subdurale elektrode-arrays in een minivarkensmodel, waarbij chirurgische methoden en hulpmiddelen, postoperatieve magnetische resonantiebeeldvorming, elektrofysiologie van de auditieve cortex, elektrochemische eigenschappen van het implantaat en postmortale immunochemie worden beschreven.

Subdurale zachte elektrocorticografie (ECoG) array-implantatie en langdurige corticale opname bij minivarkens

Dit protocol beschrijft het uitvoeren van een craniectomie met behulp van een pneumatische boor met hoge snelheid op een 3 maanden oud Deens Landras-varken. De toegang wordt gemaakt door het voorhoofdsbeen en onthult de ventrale dura mater en de onderliggende hersenhelften. Deze procedure geeft toegang tot een groot deel van de varkenshersenen.

Toegang tot het varkensbrein via high-speed pneumatische boorcraniectomie

Dit protocol beschrijft een methode om in vivo opnames van één neuron met hoge dichtheid te verkrijgen van de hersenstam van muizen met een hoofdfixatie. Deze benadering wordt gebruikt om het afvuren van het actiepotentiaal van neuronen in het ventrolaterale periaqueductale grijs te meten - een hersenstamgebied dat inactief is tijdens de Rapid Eye Movement (REM)-slaap - voor en tijdens algemene anesthesie.

Acute single-unit multi-elektrode-opnames van de hersenstam van muizen met een hoofdfixatie

Dit protocol beschrijft een tweefasige chirurgische methode om een groot, hersluitbaar, dura-sparend craniaal venster bij muizen te creëren. Deze techniek maakt chronische, multimodale elektrofysiologische opnames mogelijk van verspreide, diepe-hersennetwerken, zoals het Default Mode Network, gedurende meerdere weken.

Chirurgisch protocol voor een groot, herafsluitbaar schedelraam dat longitudinale, multimodale elektrofysiologische opnames van het muis default mode netwerk mogelijk maakt

Leer een chronische craniale venstertechniek voor herhaalde corticale opnames bij varkens. Deze methode verbetert signaalintegriteit en vermindert risico's van implantaatfalen in pijnonderzoek.

Chronische craniale venstertechniek voor herhaalde corticale opnames tijdens anesthesie bij varkens

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