Name: Gedragstesten voor optogenetische manipulatie van neurale circuits in Drosophila melanogaster (Video) | JoVE Journal
Uploaded: 2025-02-07T14:50:00.000+00:00
Duration: 7 min 39 s
Description: Leer optogenetische manipulatie van neurale circuits in Drosophila melanogaster voor gedragsbiologie. Deze methode activeert en remt specifieke neuronen om thermotactisch en gustatief gedrag te bestuderen.

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<li>Dorkenwald, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neuronal+wiring+diagram+of+an+adult+brain%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Neuronal wiring diagram of an adult brain.</a> Nature. 634 (8032), 124-138 (2024).</li>
<li>Schlegel, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Whole-brain+annotation+and+multi-connectome+cell+typing+of+drosophila%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Whole-brain annotation and multi-connectome cell typing of drosophila.</a> Nature. 634 (8032), 139-152 (2024).</li>
<li>Talay, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Transsynaptic+mapping+of+second-order+taste+neurons+in+flies+by+trans-tango%5BTitle%5D)+AND+%22Neuron%22%5BJournal%5D)">Transsynaptic mapping of second-order taste neurons in flies by trans-tango.</a> Neuron. 96 (4), 783-795.e4 (2017).</li>
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<li>Pulver, S. R., Hornstein, N. J., Land, B. L., Johnson, B. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optogenetics+in+the+teaching+laboratory%3A+Using+channelrhodopsin-2+to+study+the+neural+basis+of+behavior+and+synaptic+physiology+in+drosophila%5BTitle%5D)+AND+%22Adv+Physiol+Educ%22%5BJournal%5D)">Optogenetics in the teaching laboratory: Using channelrhodopsin-2 to study the neural basis of behavior and synaptic physiology in drosophila.</a> Adv Physiol Educ. 35 (1), 82-91 (2011).</li>
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gedragstesten voor optogenetische manipulatie van neurale circuits in drosophila melanogaster

Behavioral Assays for Optogenetic Manipulation of Neural Circuits in Drosophila melanogaster

Gedragstesten voor optogenetische manipulatie van neurale circuits in Drosophila melanogaster

Optogenetics has become a fundamental technique in neuroscience, enabling precise control of neuronal activity through light stimulation. This study ...

behavioral-assays-for-optogenetic-manipulation-neural-circuits

Research

JoVE Journal

Neuroscience

Neurowetenschap

Optogenetica maakt het gebruik van licht mogelijk om neuronen te manipuleren die genetisch zijn ontworpen om specifieke opsines-lichtgevoelige eiwitten uit te drukken waarvan de lichtactivatie een verandering in de toestand van het neuron teweegbrengt. Hier beschrijven we een optogenetische benadering in Drosophila met behulp van de opsin Channelrhodopsin2. Het voorbeeldprotocol bevat een optogenetische testset om het neuronale circuit achter het ontsnappingsgedrag van de vlieg te bestuderen.

Drosophila Optogenetica: een methode om neuronale circuits te manipuleren

This video demonstrates a study of the navigational behavior of Drosophila larvae in response to optogenetic stimulation of olfactory sensory neurons (ORNs). Transgenic Drosophila larvae expressing light-sensitive ion channels in the ORNs are placed on agarose within a behavior arena. Optogenetic stimulation with light opens the channels, causing a cation influx that triggers the transmission of the signal to the brain, and the navigation behavior is recorded.

Navigational Behavior of Drosophila Larvae in Response to Optogenetic Stimulation of Olfactory Sensory Neurons

Genetisch gecodeerd optogenetic tools kunnen niet-invasieve manipulatie van specifieke neuronen in...

Optogenetic Stimulatie van Escape Gedrag in<em&gt; Drosophila melanogaster</em

Hier beschrijven we een licht-donker voorkeur test voor Drosophila larven. Deze test geeft informatie over aangeboren en circadiane regulatie van licht sensing en verwerken photobehavior.

Lichte voorkeur Assay om Aangeboren en Circadiaanse Gereglementeerde Photobehavior studeren in<em&gt; Drosophila</em&gt; Larven

Hier een protocol beschrijven we voor optogenetic manipulatie van motoneuronal activiteit, terwijl het volgen van veranderingen in motorvermogen (spiercontractie) in...

Optogenetic Perturbation van neurale activiteit met Laser Verlichting in Semi-intacte<em&gt; Drosophila</em&gt; Larven in Motion

In deze video artikel beschrijven we een geautomatiseerde test om het effect van honger of verzadiging op olfactorische afhankelijke voedsel zoekgedrag meten de volwassen fruitvlieg Drosophila melanogaster.

A Single-fly test voor foerageergedrag in Drosophila

We beschrijven hier een gedragsopstelling en methode voor gegevensanalyse voor het meten van reukresponsen van maximaal 100 azijnvliegen (Drosophila melanogaster). Dit systeem kan worden gebruikt met enkele of meerdere reukstimuli en is aanpasbaar voor optogenetische activering of het tot zwijgen brengen van neuronale subsetten.

Olfactorische gedrag getest door Computer Tracking Of<em&gt; Drosophila</em&gt; In een vier-kwadrant olfactometer

Dit protocol analyseert navigatie gedrag van Drosophila larve in reactie op de stimulatie van de gelijktijdige optogenetic van haar olfactorische neuronen. Licht van 630 nm golflengte wordt gebruikt voor het activeren van individuele olfactorische neuronen uitdrukken van een rood-verschoven kanaal rodopsine. Larvale verkeer gelijktijdig wordt bijgehouden, digitaal opgenomen en geanalyseerd met behulp van aangepaste-geschreven software.

Bijhouden van Drosophila larvale gedrag in reactie op de stimulatie van de Optogenetic van olfactorische neuronen

Dit protocol introduceert een lichtvlek test om Drosophila larvale phototactische gedrag te onderzoeken. Bij deze test wordt een lichtvlek opgewekt als licht stimulatie en wordt het proces van het vermijden van larvale licht door een op infraroodlicht gebaseerd beeldvormings systeem vastgelegd.

Licht spot-gebaseerde assay voor analyse van Drosophila larval phototaxis

Het huidige protocol beschrijft de generatie van Drosophila melanogaster die eNpHR2.0 of ReaChR-opsinen in het hart tot expressie brengt voor OCT-beeldvorming en optogenetische hartpacing. Gedetailleerde instructies voor Drosophila OCT beeldvorming en hartslagmodulatie, inclusief de simulatie van herstelbare hartstilstand, bradycardie en tachycardie bij levende dieren in verschillende ontwikkelingsstadia, worden gerapporteerd.

Ontwikkeling van Drosophila melanogaster modellen voor beeldvorming en optogenetische controle van de hartfunctie

Het protocol presenteert een high-throughput, kosteneffectieve methode voor het beoordelen van neurotoxiciteit in de fruitvlieg door middel van de kwantificering van locomotorische disfunctie als alternatief voor traditionele zoogdiermodellen. Het heeft tot doel de neurotoxische effecten van farmaceutische verbindingen, milieuagentia of genetische modificaties te evalueren met behulp van een gevoelig, reproduceerbaar en ethisch gunstig model.

Gedragsanalyse van locomotorische disfunctie bij Drosophila melanogaster als uitlezing voor neurotoxiciteit

Leer optogenetische manipulatie van neurale circuits in Drosophila melanogaster voor gedragsbiologie. Deze methode activeert en remt specifieke neuronen om thermotactisch en gustatief gedrag te bestuderen.

Gedragstesten voor optogenetische manipulatie van neurale circuits in Drosophila melanogaster

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