Name: Identificatie van microglia en perifere infiltrerende macrofagen in het beschadigde ruggenmerg met behulp van flowcytometrie (Video) | JoVE Journal
Uploaded: 2025-06-24T13:40:00.000+00:00
Duration: 8 min 47 s
Description: Leer microglia en infiltrerende macrofagen identificeren in beschadigd ruggenmerg met geoptimaliseerde flowcytometrie. Deze methode onderscheidt M1- en M2-fenotypen voor inzicht in neuro-inflammatie.

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<table><tbody><tr><td>1&times;PBS</td><td>&nbsp;Solarbio</td><td>P1020</td><td></td></tr><tr><td>10&times;PBS</td><td>&nbsp;Solarbio</td><td>P1022</td><td></td></tr><tr><td>18 acht weken oude vrouwelijke C57/BL6 muizen&nbsp;</td><td>Cavens Laboratory Animal Ltd, Chang Zhou, China</td><td></td><td></td></tr><tr><td>APC-eFluor 780-gelabelde Rat anti-muis CCR7 (IgG2b kappa)</td><td>Invitrogen</td><td>47-1971-82</td><td>0.25&mu;g/test</td></tr><tr><td>APC-eFluor 780-gelabelde Rat IgG2b kappa&nbsp; isotype control</td><td>Invitrogen</td><td>47-4321-82</td><td>0.25&mu;g/test</td></tr><tr><td>APC-gelabelde Rat anti-muis CD45 (IgG2b kappa)</td><td>Invitrogen</td><td>17-0451-82</td><td>0.125&mu;g/test</td></tr><tr><td>APC-gelabelde Rat IgG2b kappa&nbsp; isotype control</td><td>Invitrogen</td><td>17-4031-82</td><td>0.125&mu;g/test</td></tr><tr><td>DMEM</td><td>Gibco</td><td>11965092</td><td></td></tr><tr><td>Fetaal bovien serum</td><td>Lonsera</td><td>S711-001</td><td></td></tr><tr><td>FITC-gelabelde Rat anti-muis CD68 (IgG2b kappa)</td><td>Invitrogen</td><td>&nbsp;MA5-16676</td><td>0.25&mu;g/test</td></tr><tr><td>FITC-gelabelde Rat IgG2b kappa&nbsp; isotype control</td><td>Invitrogen</td><td>11-4031-82</td><td>0.25&mu;g/test</td></tr><tr><td>Paraformaldehyde</td><td>biosharp</td><td>23319084</td><td></td></tr><tr><td>PE-gelabelde Rat anti-muis CD11b (IgG2b kappa)</td><td>Invitrogen</td><td>12-0112-81</td><td>0.25&mu;g/test</td></tr><tr><td>PE-gelabelde Rat IgG2b kappa&nbsp; isotype control</td><td>Invitrogen</td><td>12-4031-82</td><td>0.25&mu;g/test</td></tr><tr><td>Percoll</td><td>&nbsp;Solarbio</td><td>P8370</td><td></td></tr><tr><td>Trypsine oplossing</td><td>biosharp</td><td>BL526A</td><td></td></tr></tbody></table>

identificatie van microglia en perifere infiltrerende macrofagen in het beschadigde ruggenmerg met behulp van flowcytometrie

Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry

Identificatie van microglia en perifere infiltrerende macrofagen in het beschadigde ruggenmerg met behulp van flowcytometrie

In a healthy spinal cord, peripheral infiltrating macrophages are almost undetectable, and microglia (MG) participate in maintaining the stability of the ...

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Detectie van microRNA&#39;s in Microglia door Real-time PCR in Normal CNS en tijdens ontstekingsprocessen

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Stereological en flowcytometrie Karakterisering van leukocytsubpopulaties in Modellen van voorbijgaande of blijvende cerebrale ischemie

Twee-foton intravital beeldvorming kan worden gebruikt om interacties tussen verschillende celtypen in het ruggenmerg in hun oorspronkelijke weefselomgeving in een botmerg chimerisch dier met een traumatische ruggenmergverplettering in de dorsale kolom te onderzoeken.

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Analyse van Microglia en Monocyt-afgeleide Macrofagen uit het Centrale Zenuwstelsel door Flow Cytometry

Kwantitatieve analyse van de inhoud van de cel binnen de nervus ischiadicus lymfkliertest is moeilijk te wijten aan de schaarste van het weefsel. Dit protocol beschrijft een methode voor de vertering van het weefsel en voorbereiding waarmee voldoende cellen voor stroom cytometry analyse van individuele muizen immuun cel populaties van zenuwen.

Analyse van immuuncellen in één ischiadicus zenuwen en achterwortelganglia Ganglion uit een enkele muis met behulp van Stroom Cytometry

Hier presenteren we een protocol om primaire microglia te isoleren en te zuiveren in diermodellen van demyeliniserende ziekten, met behulp van kolomvormige magnetisch geactiveerde celsortering.

Isolatie van primaire microglia van muizen door magnetisch geactiveerde celsortering in diermodellen van demyelinisatie

Hier presenteren we een protocol om de immuuncelfunctie na een ischemische beroerte te beoordelen met behulp van flowcytometrische analyse in een mini-beroertemodel. Deze methode maakt gedetailleerd onderzoek van neuro-immuuninteracties mogelijk en kan worden aangepast voor het bestuderen van andere neurodegeneratieve ziekten, waardoor ons begrip van immuunresponsen bij beroertepathologie wordt vergroot.

Isolatie en flowcytometrische beoordeling van neuro-immuuninteracties in een mini-beroerte muizenmodel

Dit protocol combineert flowcytometrie en eencellige RNA-sequencing om microgliale celtoestanden in het cerebellum van vroege postnatale muizenhersenen te isoleren en te karakteriseren. Het maakt gebruik van enzymatische dissociatie, Percoll-centrifugatie en immunokleuring om de heterogeniteit van microglia's te onthullen en het begrip van hun rol in de ontwikkeling en ziekte van cerebellaire patiënten te verbeteren.

Flowcytometrie en eencellige analyse voor het karakteriseren van microglia-activering bij vroege postnatale hersenontwikkeling bij muizen

Leer microglia en infiltrerende macrofagen identificeren in beschadigd ruggenmerg met geoptimaliseerde flowcytometrie. Deze methode onderscheidt M1- en M2-fenotypen voor inzicht in neuro-inflammatie.

Identificatie van microglia en perifere infiltrerende macrofagen in het beschadigde ruggenmerg met behulp van flowcytometrie

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