Name: Isolatie en kweek van primaire retinale Müller-cellen van Sprague-Dawley (SD) ratten (Video) | JoVE Journal
Uploaded: 2025-06-17T14:20:00.000+00:00
Duration: 7 min 41 s
Description: Leer de isolatie en kweek van primaire retinale Müller-cellen uit neonatale Sprague-Dawley ratten. Deze methode biedt een gestandaardiseerd model voor retinale ziekteonderzoek en geneesmiddelstudies.

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<li>Liu, X., Tang, L., Liu, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mouse+M%C3%BCller+cell+isolation+and+culture%5BTitle%5D)+AND+%22Bio+Protoc%22%5BJournal%5D)">Mouse Müller cell isolation and culture.</a> Bio Protoc. 7 (15), e2429(2017).</li>
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<li>Wang, M., Ma, W., Zhao, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adaptive+M%C3%BCller+cell+responses+to+microglial+activation+mediate+neuroprotection+and+coordinate+inflammation+in+the+retina%5BTitle%5D)+AND+%22J+Neuroinflammation%22%5BJournal%5D)">Adaptive Müller cell responses to microglial activation mediate neuroprotection and coordinate inflammation in the retina.</a> J Neuroinflammation. 8, 173(2011).</li>
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<li>Li, Q., Cheng, Y., Zhang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((TRPV4-induced+M%C3%BCller+cell+gliosis+and+TNF-%CE%B1+elevation-mediated+retinal+ganglion+cell+apoptosis+in+glaucomatous+rats+via+JAK2%2FSTAT3%2FNF-%CE%BAB+pathway%5BTitle%5D)+AND+%22J+Neuroinflammation%22%5BJournal%5D)">TRPV4-induced Müller cell gliosis and TNF-α elevation-mediated retinal ganglion cell apoptosis in glaucomatous rats via JAK2/STAT3/NF-κB pathway.</a> J Neuroinflammation. 18 (1), 271(2021).</li>
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<li>Harstad, H. K., Ringvold, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Scanning+and+transmission+electron+microscopy+of+M%C3%BCller+cells+isolated+from+rabbit+retina%5BTitle%5D)+AND+%22Graefes+Arch+Clin+Exp+Ophthalmol%22%5BJournal%5D)">Scanning and transmission electron microscopy of Müller cells isolated from rabbit retina.</a> Graefes Arch Clin Exp Ophthalmol. 223 (1), 29-34 (1985).</li>
<li>Guidry, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+and+characterization+of+porcine+M%C3%BCller+cells.+Myofibroblastic+dedifferentiation+in+culture%5BTitle%5D)+AND+%22Invest+Ophthalmol+Vis+Sci%22%5BJournal%5D)">Isolation and characterization of porcine Müller cells. Myofibroblastic dedifferentiation in culture.</a> Invest Ophthalmol Vis Sci. 37 (5), 740-752 (1996).</li>
<li>Qiu, A. W., Wang, N. Y., Yin, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Retinal+M%C3%BCller+cell-released+exosomal+miR-92a-3p+delivers+interleukin-17A+signal+by+targeting+Notch-1+to+promote+diabetic+retinopathy%5BTitle%5D)+AND+%22Invest+Ophthalmol+Vis+Sci%22%5BJournal%5D)">Retinal Müller cell-released exosomal miR-92a-3p delivers interleukin-17A signal by targeting Notch-1 to promote diabetic retinopathy.</a> Invest Ophthalmol Vis Sci. 66 (1), 1(2025).</li>
</ol>

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isolatie en kweek van primaire retinale müller-cellen van sprague-dawley (sd) ratten

Isolation and Culture of Primary Retinal M&#252;ller Cells from Sprague-Dawley (SD) Rats

Isolatie en kweek van primaire retinale Müller-cellen van Sprague-Dawley (SD) ratten

Retinal M&#252;ller cells (RMCs) play a crucial role in providing structural support and regulating various functions within the retina. As a core ...

isolation-culture-primary-retinal-mller-cells-from-sprague-dawley-sd

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De retinale pigment epitheel (RPE) is een multi-functionele epitheel van het oog. Hier presenteren we een protocol om primaire celculturen afgeleid van de lymfkliertest RPE.

Primaire celculturen van de muis retinale Pigment epitheel

Dit protocol, dat oorspronkelijk werd gerapporteerd door Fernandez-Godino et al. in 20161, beschrijft een methode om RPE-cellen van muizen efficiënt te isoleren en te cultiseren, die binnen een week een functionele en gepolariseerde RPE-monolaag vormen op Transwell-platen. De procedure duurt ongeveer 3 uur.

Efficiënte dissectie en cultuur van primaire muis retinale pigment epitheelcellen

Dit manuscript beschrijft een vereenvoudigd protocol voor de isolatie van retinale gepigmenteerde epitheelcellen (RPE) uit muizenogen op een stapsgewijze manier. Het protocol omvat de enucleatie en dissectie van muizenogen, gevolgd door de isolatie, zaaiing en kweek van RPE-cellen.

Isolatie van primaire retinale gepigmenteerde epitheelcellen van muizen

Dit protocol schetst de procedure voor het verkrijgen en kweken van primaire retinale pigmentepitheelcellen (RPE) uit lokaal geproduceerde varkensogen. Deze cellen dienen als hoogwaardig alternatief voor stamcellen en zijn geschikt voor in vitro netvliesonderzoek.

Isolatie van primaire retinale pigmentepitheelcellen van varkens voor in vitro modellering

Dit manuscript beschrijft een gedetailleerd protocol voor het isoleren van retinale gliacellen Müller uit muizenogen. Het protocol begint met enucleatie en dissectie van muizenogen, gevolgd door isolatie, zaaien en kweken van Müller-cellen.

Isolatie van primaire müllercellen van de retinale glia van muizen

Retinaal pigmentepitheel (RPE) fungeert als een cruciale barrière tussen het vaatvlies en het netvlies en bevordert de gezondheid en functie van retinale celtypen, zoals fotoreceptoren. Hierin beschrijven we een eenvoudig en effectief protocol voor het isoleren en kweken van RPE van volwassen muizen.

Een vereenvoudigde methode voor isolatie en kweek van retinale pigmentepitheelcellen van volwassen muizen

De dissectie en de groei van cellen van een persoon hersengebied vergemakkelijkt onderzoek van cellulaire en fysiologische parameters. We beschrijven een methode voor primaire celcultuur dat neuron verrijkte culturen produceert in een serum-vrije omgeving.

Primaire Cultuur van hippocampale neuronen van P0 pasgeboren ratten

Primaire hepatocyten een waardevol hulpmiddel om biochemische, moleculaire en metabolische functies in een fysiologisch relevante experimentele systeem te evalueren. We beschrijven een betrouwbaar protocol voor rat in situ leverperfusie, die consequent levensvatbare hepatocyten genereert tot to1.0 ×...

Isolatie en Primaire Cultuur van Rat levercellen

Leer de isolatie en kweek van primaire retinale Müller-cellen uit neonatale Sprague-Dawley ratten. Deze methode biedt een gestandaardiseerd model voor retinale ziekteonderzoek en geneesmiddelstudies.

Isolatie en kweek van primaire retinale Müller-cellen van Sprague-Dawley (SD) ratten

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