Name: Anesthesievrije hartslagmetingen bij vrij bewegende zebravissen (Video) | JoVE Journal
Uploaded: 2025-04-18T14:40:00.000+00:00
Duration: 3 min 57 s
Description: Leer een niet-invasieve methode voor het meten van hartslag bij vrij bewegende zebravissen zonder verdoving. Deze techniek maakt gebruik van shortwave infrared imaging en neurale netwerken voor nauwkeurige cardiale monitoring.

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<li>Chen, X., Li, Y., Yao, T., Jia, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Benefits+of+zebrafish+xenograft+models+in+cancer+research%5BTitle%5D)+AND+%22Front+Cell+Dev+Biol%22%5BJournal%5D)">Benefits of zebrafish xenograft models in cancer research.</a> Front Cell Dev Biol. 9, 616551(2021).</li>
<li>Lai, K. P., Gong, Z., Tse, W. K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Zebrafish+as+the+toxicant+screening+model%3A+Transgenic+and+omics+approaches%5BTitle%5D)+AND+%22Aquat+Toxicol%22%5BJournal%5D)">Zebrafish as the toxicant screening model: Transgenic and omics approaches.</a> Aquat Toxicol. 234, 105813(2021).</li>
<li>Pylatiuk, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Automatic+zebrafish+heartbeat+detection+and+analysis+for+zebrafish+embryos%5BTitle%5D)+AND+%22Zebrafish%22%5BJournal%5D)">Automatic zebrafish heartbeat detection and analysis for zebrafish embryos.</a> Zebrafish. 11 (4), 379-383 (2014).</li>
<li>Krishna, S., Chatti, K., Galigekere, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Automatic+and+robust+estimation+of+heart+rate+in+zebrafish+larvae%5BTitle%5D)+AND+%22IEEE+Trans+Auto+Sci+Eng%22%5BJournal%5D)">Automatic and robust estimation of heart rate in zebrafish larvae.</a> IEEE Trans Auto Sci Eng. 15 (3), 1041-1052 (2018).</li>
<li>Gierten, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Automated+high-throughput+heartbeat+quantification+in+medaka+and+zebrafish+embryos+under+physiological+conditions%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Automated high-throughput heartbeat quantification in medaka and zebrafish embryos under physiological conditions.</a> Sci Rep. 10 (1), 2046(2020).</li>
<li>Meng, H., Liang, J., Zheng, X., Zhang, K., Zhao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+a+high-throughput+zebrafish+embryo+screening+approach+to+support+environmental+hazard+ranking+for+cardiovascular+agents%5BTitle%5D)+AND+%22Sci+Total+Environ%22%5BJournal%5D)">Using a high-throughput zebrafish embryo screening approach to support environmental hazard ranking for cardiovascular agents.</a> Sci Total Environ. 702, 134703(2020).</li>
<li>Krylov, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+hypomagnetic+field+on+the+heartbeat+in+zebrafish+embryos%5BTitle%5D)+AND+%22Front+Physiol%22%5BJournal%5D)">Influence of hypomagnetic field on the heartbeat in zebrafish embryos.</a> Front Physiol. 13, 1040083(2022).</li>
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<li>Dhillon, S. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optimisation+of+embryonic+and+larval+ECG+measurement+in+Zebrafish+for+quantifying+the+effect+of+QT+prolonging+drugs%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Optimisation of embryonic and larval ECG measurement in Zebrafish for quantifying the effect of QT prolonging drugs.</a> PLoS One. 8 (4), e60552(2013).</li>
<li>Bedrossiantz, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Heart+rate+and+behavioral+responses+in+three+phylogenetically+distant+aquatic+model+organisms+exposed+to+environmental+concentrations+of+carbaryl+and+fenitrothion%5BTitle%5D)+AND+%22Sci+Total+Environ%22%5BJournal%5D)">Heart rate and behavioral responses in three phylogenetically distant aquatic model organisms exposed to environmental concentrations of carbaryl and fenitrothion.</a> Sci Total Environ. 865, 161268(2023).</li>
<li>Santoso, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((An+overview+of+methods+for+cardiac+rhythm+detection+in+zebrafish%5BTitle%5D)+AND+%22Biomedicines%22%5BJournal%5D)">An overview of methods for cardiac rhythm detection in zebrafish.</a> Biomedicines. 8 (9), 329(2020).</li>
<li>Ling, D., Chen, H., Chan, G., Lee, S. M. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+measurements+of+zebrafish+heartrate+and+heart+rate+variability%3A+A+survey+between+1990-2020%5BTitle%5D)+AND+%22Comp+Biol+Med%22%5BJournal%5D)">Quantitative measurements of zebrafish heartrate and heart rate variability: A survey between 1990-2020.</a> Comp Biol Med. 142, 105045(2022).</li>
<li>Ferreira, M. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((FEHAT%3A+Efficient%2C+large+scale+and+automated+heartbeat+detection+in+medaka+fish+embryos%5BTitle%5D)+AND+%22Bioinformatics%22%5BJournal%5D)">FEHAT: Efficient, large scale and automated heartbeat detection in medaka fish embryos.</a> Bioinformatics. 40 (12), btae664(2024).</li>
<li>Volkov, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Optical+transparency+and+label-free+vessel+imaging+of+zebrafish+larvae+in+shortwave+infrared+range+as+a+tool+for+prolonged+studying+of+cardiovascular+system+development%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Optical transparency and label-free vessel imaging of zebrafish larvae in shortwave infrared range as a tool for prolonged studying of cardiovascular system development.</a> Sci Rep. 12 (1), 20884(2022).</li>
<li>Machikhin, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microscopic+photoplethysmography-based+evaluation+of+cardiotoxicity+in+whitefish+larvae+induced+by+acute+exposure+to+cadmium+and+phenol%5BTitle%5D)+AND+%22J+Biophoton%22%5BJournal%5D)">Microscopic photoplethysmography-based evaluation of cardiotoxicity in whitefish larvae induced by acute exposure to cadmium and phenol.</a> J Biophoton. 17 (9), e202400111(2024).</li>
<li>Machikhin, A. S., Burlakov, A. B., Volkov, M. V., Khokhlov, D. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Imaging+photoplethysmography+and+videocapillaroscopy+enable+noninvasive+study+of+zebrafish+cardiovascular+system+functioning%5BTitle%5D)+AND+%22J+Biophoton%22%5BJournal%5D)">Imaging photoplethysmography and videocapillaroscopy enable noninvasive study of zebrafish cardiovascular system functioning.</a> J Biophoton. 13 (7), e202000061(2020).</li>
<li>Denvir, M. A., Tucker, C. S., Mullins, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Systolic+and+diastolic+ventricular+function+in+zebrafish+embryos%3A+Influence+of+norepenephrine%2C+MS-222+and+temperature%5BTitle%5D)+AND+%22BMC+Biotechnol%22%5BJournal%5D)">Systolic and diastolic ventricular function in zebrafish embryos: Influence of norepenephrine, MS-222 and temperature.</a> BMC Biotechnol. 8 (1), 1-8 (2008).</li>
<li>Santoso, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+simple+ImageJ-based+method+for+dynamic+blood+flow+tracking+in+zebrafish+embryos+and+its+application+in+drug+toxicity+evaluation%5BTitle%5D)+AND+%22Inventions%22%5BJournal%5D)">Development of a simple ImageJ-based method for dynamic blood flow tracking in zebrafish embryos and its application in drug toxicity evaluation.</a> Inventions. 4 (4), 65(2019).</li>
<li>Vieira, R. S. F., Sousa, D., Félix, L. M., Venâncio, C. A. S. Anaesthetic profile of thymol and menthol in zebrafish larvae model. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aVieira%2C+RSF+%22Aquaculture+and+Fisheries%22">Aquaculture and Fisheries</a>. , (2024).</li>
<li>Westerfield, M. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aWesterfield%2C+M+%22The+zebrafish+book.+A+guide+for+the+laboratory+use+of+zebrafish+%28Danio+rerio%29%22">The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio)</a>. , 4th ed, Univ. of Oregon Press. Eugene. (2000).</li>
<li>Azad, R., et al. Loss functions in the era of semantic segmentation: A survey and outlook. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aAzad%2C+R+%22ArXiv%22">ArXiv</a>. , (2023).</li>
<li>Machikhin, A. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Combined+optical+and+acoustic+microscopy+for+non-invasive+cardiovascular+studies+using+zebrafish+model%5BTitle%5D)+AND+%22IEEE+Trans+Instrument+Measurement%22%5BJournal%5D)">Combined optical and acoustic microscopy for non-invasive cardiovascular studies using zebrafish model.</a> IEEE Trans Instrument Measurement. 74, 1-13 (2025).</li>
<li>Wavelet analysis of Photoplethysmogram in zebrafish larvaе cardiovascular system Study. Slavin, A. E., Guryleva, A. V., Bukova, V. I., Shuman, L. A., Burlakov, A. B. 2024 26th Int Conf Digital Signal Proc Appl DSPA 2024, , 1-5 (2024).</li>
<li>Kumari, S., Singh, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Phenylthiourea-mediated+experimental+depigmentation+reduces+seizurogenic+response+of+pentylenetetrazol+in+zebrafish+larva%5BTitle%5D)+AND+%22J+Pharm+Toxicol+Methods%22%5BJournal%5D)">Phenylthiourea-mediated experimental depigmentation reduces seizurogenic response of pentylenetetrazol in zebrafish larva.</a> J Pharm Toxicol Methods. 128, 107532(2024).</li>
<li>Xu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Highly+sensitive+response+to+the+toxicity+of+environmental+chemicals+in+transparent+casper+zebrafish%5BTitle%5D)+AND+%22Sci+Total+Environ%22%5BJournal%5D)">Highly sensitive response to the toxicity of environmental chemicals in transparent casper zebrafish.</a> Sci Total Environ. 948, 174865(2024).</li>
<li>Vedder, V. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((pyHeart4Fish%3A+Chamber-specific+heart+phenotype+quantification+of+zebrafish+in+high-content+screens%5BTitle%5D)+AND+%22Front+Cell+Dev+Biol%22%5BJournal%5D)">pyHeart4Fish: Chamber-specific heart phenotype quantification of zebrafish in high-content screens.</a> Front Cell Dev Biol. 11, 1143852(2023).</li>
<li>Lane, S., More, L. A., Asnani, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Zebrafish+models+of+cancer+therapy-induced+cardiovascular+toxicity%5BTitle%5D)+AND+%22J+Cardiovas+Dev+Dis%22%5BJournal%5D)">Zebrafish models of cancer therapy-induced cardiovascular toxicity.</a> J Cardiovas Dev Dis. (2), 8(2021).</li>
<li>Maciag, M., Wnorowski, A., Mierzejewska, M., Plazinska, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pharmacological+assessment+of+zebrafish-based+cardiotoxicity+models%5BTitle%5D)+AND+%22Biomed+pharmacother%22%5BJournal%5D)">Pharmacological assessment of zebrafish-based cardiotoxicity models.</a> Biomed pharmacother. 148, 112695(2022).</li>
<li>Krylov, V. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-invasive+recording+of+heartbeats+in+Danio+rerio+and+Daphnia+magna+to+assess+the+toxicity+of+imidacloprid+and+glyphosate%5BTitle%5D)+AND+%22Comp+Biochem+Physiol+C+Toxicol+Pharmacol%22%5BJournal%5D)">Non-invasive recording of heartbeats in Danio rerio and Daphnia magna to assess the toxicity of imidacloprid and glyphosate.</a> Comp Biochem Physiol C Toxicol Pharmacol. 288, 110075(2025).</li>
<li>Meng, H., Liang, J., Zheng, X., Zhang, K., Zhao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+a+high-throughput+zebrafish+embryo+screening+approach+to+support+environmental+hazard+ranking+for+cardiovascular+agents%5BTitle%5D)+AND+%22Sci+Total+Environ%22%5BJournal%5D)">Using a high-throughput zebrafish embryo screening approach to support environmental hazard ranking for cardiovascular agents.</a> Sci Total Environ. 702, 134703(2020).</li>
</ol>

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```

anesthesievrije hartslagmetingen bij vrij bewegende zebravissen

Anesthesia-free Heartbeat Measurements in Freely Moving Zebrafish

Anesthesievrije hartslagmetingen bij vrij bewegende zebravissen

Zebrafish (Danio rerio) is a widely used model organism in physiological, pharmacological, and toxicological research due to its genetic similarity to ...

anesthesia-free-heartbeat-measurements-in-freely-moving-zebrafish

Research

JoVE Journal

Bioengineering

Bio-engineering

Hebben we een semi-automatische Optical Heartbeat Analysis-methode (Soha) voor het analyseren van snelle optische opnamen...

Semi-automatische Optical Heartbeat Analyse van kleine hart

This video shows how to perform electrocardiography (ECG or EKG) measurements, a test which measures the electrical activity of the heart, on a zebrafish.

Zebrafish Electrocardiography (ECG): A Minimally Invasive Assay to Evaluate Cardiac Function

Dit document beschrijft hoe een volwassen zebravissen kunnen worden geïmmobiliseerd, geïntubeerd, en gebruikt voor in vivo elektrofysiologische experimenten opnamen en manipulatie van neurale activiteit in een intact dier mogelijk maken.

Elektrofysiologische opname in de hersenen van Intact Adult zebravis

Het gebruik van zebravissen voor cardiovasculair onderzoek breidt zich uit naar onderzoek naar volwassen harten. Voor deze toepassingen is snelle en eenvoudige isolatie van hartweefsels essentieel om postmortale veranderingen te voorkomen en om een voldoende aantal monsters te verkrijgen. Hier beschrijven we een snelle en reproduceerbare methode voor het dissecteren van harten van volwassen zebravissen.

Herstel van de Adult zebravis Harten voor High-throughput Toepassingen

Hier presenteren we een betrouwbare, minimaal invasieve en kosteneffectieve methode om elektrocardiogrammen op te nemen en te interpreteren in levende verdoemde volwassen zebravis.

In vivo oppervlakte elektrocardiografie voor volwassen zebravis

Dit artikel beschrijft een methode voor het monteren van fragiele zebravis embryo's voor uitgebreide time-lapse confocale microscopie. Deze voordelige methode is eenvoudig uit te voeren met behulp van regelmatige glazen bodem microscopie gerechten voorbeeld vorming op elke omgekeerde Microscoop. De montage wordt uitgevoerd in lagen van agarose bij verschillende concentraties.

Een gelaagde montagemethode voor de uitgebreide time-lapse confocale microscopie van hele zebravis embryo's

We beschrijven een protocol om hartmorfologie en functie bij volwassen zebravissen te beoordelen met behulp van hoogfrequente echocardiografie. De methode maakt visualisatie van het hart en de daaropvolgende kwantificering van functionele parameters mogelijk, zoals hartslag (HR), hartoutput (CO), fractionele gebiedsverandering (FAC), uitwerpfractie (EF) en bloedinstroom en uitstroomsnelheden.

High-Frequency Ultrasound Echocardiography om zebravissen cardiale functie te beoordelen

We beschrijven geoptimaliseerde hulpmiddelen om het hart van de zebravis in vivo te bestuderen met fluorescentiemicroscopie van lichtplaten. In het bijzonder stellen we heldere cardiale transgene lijnen voor en presenteren we nieuwe zachte inbeddings- en immobilisatietechnieken die ontwikkelings- en hartafwijkingen voorkomen. Een mogelijke data-acquisitie- en analysepijplijn aangepast aan cardiale beeldvorming wordt ook verstrekt.

Light Sheet Microscopie van snelle hartdynamica in zebravisembryo's

Dit protocol maakt gebruik van light-sheet beeldvorming om de contractiele functie van het hart bij zebravislarven te onderzoeken en inzicht te krijgen in de hartmechanica door middel van celtracking en interactieve analyse.

4D Light-sheet beeldvorming van hartcontractie van zebravissen

De studie introduceert Zebra II, een geavanceerd systeem voor langdurige ECG-acquisitie en analyse bij zebravissen. Dit systeem beschikt over een onafhankelijk perfusiesysteem en meerpuntselektroden voor het behandelen van meerdere vissen in gecontroleerde omgevingen. De acute effecten van Amiodarone werden beoordeeld en geanalyseerd met wild-type zebravissen.

Zebra II als een nieuw systeem om elektrofysiologische signalen bij zebravissen op te nemen

Dit protocol beschrijft een goedkope lichtmicroscopiemethode om de cardiale morfologie en functie in zebravisembryo's te beoordelen, waardoor een reproduceerbare evaluatie van ontwikkelingscardiotoxiciteit mogelijk is zonder dat er geavanceerde beeldvormingssystemen nodig zijn.

Functionele cardiale beeldvorming in zebravisembryo's met behulp van standaardmicroscopie en videoanalyse: toepassingen in milieu- en biomedisch onderzoek

Leer een niet-invasieve methode voor het meten van hartslag bij vrij bewegende zebravissen zonder verdoving. Deze techniek maakt gebruik van shortwave infrared imaging en neurale netwerken voor nauwkeurige cardiale monitoring.

Anesthesievrije hartslagmetingen bij vrij bewegende zebravissen

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