Name: Frequentie en distributie van cross-overs in Caenorhabditis elegans Meiose door SNP-genotypering met behulp van real-time PCR (Video) | JoVE Journal
Uploaded: 2025-07-11T15:30:00.000+00:00
Duration: 6 min 18 s
Description: Leer SNP-genotypering met real-time PCR om crossoverfrequentie en -distributie te meten tijdens meiose in Caenorhabditis elegans. Deze methode verbetert de nauwkeurigheid van genetisch onderzoek.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Jones, G., Kleckner, N., Zickler, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Meiosis+through+three+centuries%5BTitle%5D)+AND+%22Chromosoma%22%5BJournal%5D)">Meiosis through three centuries.</a> Chromosoma. 133 (2), 93-115 (2024).</li>
<li>Hassold, T. J., Hunt, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Missed+connections%3A+Recombination+and+human+aneuploidy%5BTitle%5D)+AND+%22Prenat+Diagn%22%5BJournal%5D)">Missed connections: Recombination and human aneuploidy.</a> Prenat Diagn. 41 (5), 584-590 (2021).</li>
<li>Barnes, T. M., Kohara, Y., Coulson, A., Hekimi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Meiotic+recombination%2C+noncoding+DNA+and+genomic+organization+in+caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Meiotic recombination, noncoding DNA and genomic organization in caenorhabditis elegans.</a> Genetics. 141 (1), 159-179 (1995).</li>
<li>Lim, J. G., Stine, R. R., Yanowitz, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Domain-specific+regulation+of+recombination+in+Caenorhabditis+elegans+in+response+to+temperature%2C+age%2C+and+sex%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Domain-specific regulation of recombination in Caenorhabditis elegans in response to temperature, age, and sex.</a> Genetics. 180 (2), 715-726 (2008).</li>
<li>Rockman, M. V., Kruglyak, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recombinational+landscape+and+population+genomics+of+Caenorhabditis+elegans%5BTitle%5D)+AND+%22PLoS+Genet%22%5BJournal%5D)">Recombinational landscape and population genomics of Caenorhabditis elegans.</a> PLoS Genet. 5 (3), e1000419(2009).</li>
<li>Brenner, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+genetics+of+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">The genetics of Caenorhabditis elegans.</a> Genetics. 77 (1), 71-94 (1974).</li>
<li>Li, W., Yanowitz, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Atm+and+atr+influence+meiotic+crossover+formation+through+antagonistic+and+overlapping+functions+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Atm and atr influence meiotic crossover formation through antagonistic and overlapping functions in Caenorhabditis elegans.</a> Genetics. 212 (2), 431-443 (2019).</li>
<li>Rose, A. M., Baillie, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+effect+of+temperature+and+parental+age+on+recombination+and+nondisjunction+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">The effect of temperature and parental age on recombination and nondisjunction in Caenorhabditis elegans.</a> Genetics. 92 (2), 409-418 (1979).</li>
<li>Villeneuve, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+cis-acting+locus+that+promotes+crossing+over+between+X+chromosomes+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans.</a> Genetics. 136 (3), 887-902 (1994).</li>
<li>Youds, J. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rtel-1+enforces+meiotic+crossover+interference+and+homeostasis%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Rtel-1 enforces meiotic crossover interference and homeostasis.</a> Science. 327 (5970), 1254-1258 (2010).</li>
<li>Emmons, S. W., Yesner, L., Ruan, K. S., Katzenberg, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evidence+for+a+transposon+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Evidence for a transposon in Caenorhabditis elegans.</a> Cell. 32 (1), 55-65 (1983).</li>
<li>Williams, B. D., Schrank, B., Huynh, C., Shownkeen, R., Waterston, R. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+genetic+mapping+system+in+Caenorhabditis+elegans+based+on+polymorphic+sequence-tagged+sites%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites.</a> Genetics. 131 (3), 609-624 (1992).</li>
<li>Hillers, K. J., Villeneuve, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chromosome-wide+control+of+meiotic+crossing+over+in+C.+elegans%5BTitle%5D)+AND+%22Curr+Biol%22%5BJournal%5D)">Chromosome-wide control of meiotic crossing over in C. elegans.</a> Curr Biol. 13 (18), 1641-1647 (2003).</li>
<li>Wicks, S. R., Yeh, R. T., Gish, W. R., Waterston, R. H., Plasterk, R. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+gene+mapping+in+Caenorhabditis+elegans+using+a+high-density+polymorphism+map%5BTitle%5D)+AND+%22Nat+Genet%22%5BJournal%5D)">Rapid gene mapping in Caenorhabditis elegans using a high-density polymorphism map.</a> Nat Genet. 28 (2), 160-164 (2001).</li>
<li>Campos, T. L., Korhonen, P. K., Sternberg, P. W., Gasser, R. B., Young, N. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Predicting+gene+essentiality+in+Caenorhabditis+elegans+by+feature+engineering+and+machine-learning%5BTitle%5D)+AND+%22Comput+Struct+Biotechnol+J%22%5BJournal%5D)">Predicting gene essentiality in Caenorhabditis elegans by feature engineering and machine-learning.</a> Comput Struct Biotechnol J. 18, 1093-1102 (2020).</li>
<li>Fay, D., Bender, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Snps%3A+Introduction+and+two-point+mapping%5BTitle%5D)+AND+%22WormBook%22%5BJournal%5D)">Snps: Introduction and two-point mapping.</a> WormBook. , (2008).</li>
<li>Hwang, H. Y., Wang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fast+genetic+mapping+using+insertion-deletion+polymorphisms+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans.</a> Sci Rep. 11 (1), 11017(2021).</li>
<li>Davis, M. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+single+nucleotide+polymorphism+mapping+in+C.+elegans%5BTitle%5D)+AND+%22BMC+Genomics%22%5BJournal%5D)">Rapid single nucleotide polymorphism mapping in C. elegans.</a> BMC Genomics. 6, 118(2005).</li>
<li>Livak, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Allelic+discrimination+using+fluorogenic+probes+and+the+5%27+nuclease+assay%5BTitle%5D)+AND+%22Genet+Anal%22%5BJournal%5D)">Allelic discrimination using fluorogenic probes and the 5' nuclease assay.</a> Genet Anal. 14 (5-6), 143-149 (1999).</li>
<li>Li, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+tumor+suppressor+BRCA1-BARD1+complex+localizes+to+the+synaptonemal+complex+and+regulates+recombination+under+meiotic+dysfunction+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22PLoS+Genet%22%5BJournal%5D)">The tumor suppressor BRCA1-BARD1 complex localizes to the synaptonemal complex and regulates recombination under meiotic dysfunction in Caenorhabditis elegans.</a> PLoS Genet. 14 (11), e1007701(2018).</li>
<li>Saito, T. T., Lui, D. Y., Kim, H. M., Meyer, K., Colaiacovo, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Interplay+between+structure-specific+endonucleases+for+crossover+control+during+Caenorhabditis+elegans+meiosis%5BTitle%5D)+AND+%22PLoS+Genet%22%5BJournal%5D)">Interplay between structure-specific endonucleases for crossover control during Caenorhabditis elegans meiosis.</a> PLoS Genet. 9 (7), e1003586(2013).</li>
<li>Saito, T. T., Youds, J. L., Boulton, S. J., Colaiacovo, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Caenorhabditis+elegans+him-18%2Fslx-4+interacts+with+slx-1+and+xpf-1+and+maintains+genomic+integrity+in+the+germline+by+processing+recombination+intermediates%5BTitle%5D)+AND+%22PLoS+Genet%22%5BJournal%5D)">Caenorhabditis elegans him-18/slx-4 interacts with slx-1 and xpf-1 and maintains genomic integrity in the germline by processing recombination intermediates.</a> PLoS Genet. 5 (11), e1000735(2009).</li>
<li>Longo, M. C., Berninger, M. S., Hartley, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Use+of+uracil+DNA+glycosylase+to+control+carry-over+contamination+in+polymerase+chain+reactions%5BTitle%5D)+AND+%22Gene%22%5BJournal%5D)">Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions.</a> Gene. 93 (1), 125-128 (1990).</li>
<li>Kutyavin, I. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3%27-minor+groove+binder-DNA+probes+increase+sequence+specificity+at+pcr+extension+temperatures%5BTitle%5D)+AND+%22Nucleic+Acids+Res%22%5BJournal%5D)">3'-minor groove binder-DNA probes increase sequence specificity at pcr extension temperatures.</a> Nucleic Acids Res. 28 (2), 655-661 (2000).</li>
<li>Hammarlund, M., Davis, M. W., Nguyen, H., Dayton, D., Jorgensen, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Heterozygous+insertions+alter+crossover+distribution+but+allow+crossover+interference+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Heterozygous insertions alter crossover distribution but allow crossover interference in Caenorhabditis elegans.</a> Genetics. 171 (3), 1047-1056 (2005).</li>
<li>Hodgkin, J., Horvitz, H. R., Brenner, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nondisjunction+mutants+of+the+nematode+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Nondisjunction mutants of the nematode Caenorhabditis elegans.</a> Genetics. 91 (1), 67-94 (1979).</li>
<li>Meneely, P. M., Farago, A. F., Kauffman, T. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Crossover+distribution+and+high+interference+for+both+the+X+chromosome+and+an+autosome+during+oogenesis+and+spermatogenesis+in+Caenorhabditis+elegans%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Crossover distribution and high interference for both the X chromosome and an autosome during oogenesis and spermatogenesis in Caenorhabditis elegans.</a> Genetics. 162 (3), 1169-1177 (2002).</li>
<li>Nabeshima, K., Villeneuve, A. M., Hillers, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chromosome-wide+regulation+of+meiotic+crossover+formation+in+Caenorhabditis+elegans+requires+properly+assembled+chromosome+axes%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Chromosome-wide regulation of meiotic crossover formation in Caenorhabditis elegans requires properly assembled chromosome axes.</a> Genetics. 168 (3), 1275-1292 (2004).</li>
<li>Phillips, C. M., Dernburg, A. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+family+of+zinc-finger+proteins+is+required+for+chromosome-specific+pairing+and+synapsis+during+meiosis+in+C.+elegans%5BTitle%5D)+AND+%22Dev+Cell%22%5BJournal%5D)">A family of zinc-finger proteins is required for chromosome-specific pairing and synapsis during meiosis in C. elegans.</a> Dev Cell. 11 (6), 817-829 (2006).</li>
<li>Mancera, E., Bourgon, R., Brozzi, A., Huber, W., Steinmetz, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-resolution+mapping+of+meiotic+crossovers+and+non-crossovers+in+yeast%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">High-resolution mapping of meiotic crossovers and non-crossovers in yeast.</a> Nature. 454 (7203), 479-485 (2008).</li>
<li>Qi, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+meiotic+crossovers+and+gene+conversion+by+whole-genome+sequencing+in+Saccharomyces+cerevisiae%5BTitle%5D)+AND+%22BMC+Genomics%22%5BJournal%5D)">Characterization of meiotic crossovers and gene conversion by whole-genome sequencing in Saccharomyces cerevisiae.</a> BMC Genomics. 10, 475(2009).</li>
<li>Miller, D. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+whole-chromosome+analysis+of+meiotic+recombination+in+Drosophila+melanogaster%5BTitle%5D)+AND+%22G3+%28Bethesda%29%22%5BJournal%5D)">A whole-chromosome analysis of meiotic recombination in Drosophila melanogaster.</a> G3 (Bethesda). 2 (2), 249-260 (2012).</li>
<li>Carlton, P. M., Farruggio, A. P., Dernburg, A. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+link+between+meiotic+prophase+progression+and+crossover+control%5BTitle%5D)+AND+%22PLoS+Genet%22%5BJournal%5D)">A link between meiotic prophase progression and crossover control.</a> PLoS Genet. 2 (2), e12(2006).</li>
<li>Tsai, C. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Meiotic+crossover+number+and+distribution+are+regulated+by+a+dosage+compensation+protein+that+resembles+a+condensin+subunit%5BTitle%5D)+AND+%22Genes+Dev%22%5BJournal%5D)">Meiotic crossover number and distribution are regulated by a dosage compensation protein that resembles a condensin subunit.</a> Genes Dev. 22 (2), 194-211 (2008).</li>
<li>Bhalla, N., Wynne, D. J., Jantsch, V., Dernburg, A. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Zhp-3+acts+at+crossovers+to+couple+meiotic+recombination+with+synaptonemal+complex+disassembly+and+bivalent+formation+in+C.+elegans%5BTitle%5D)+AND+%22PLoS+Genet%22%5BJournal%5D)">Zhp-3 acts at crossovers to couple meiotic recombination with synaptonemal complex disassembly and bivalent formation in C. elegans.</a> PLoS Genet. 4 (10), e1000235(2008).</li>
<li>Yokoo, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cosa-1+reveals+robust+homeostasis+and+separable+licensing+and+reinforcement+steps+governing+meiotic+crossovers%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Cosa-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers.</a> Cell. 149 (1), 75-87 (2012).</li>
<li>Almanzar, D. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Meiotic+DNA+exchanges+in+C.+elegans+are+promoted+by+proximity+to+the+synaptonemal+complex%5BTitle%5D)+AND+%22Life+Sci+Alliance%22%5BJournal%5D)">Meiotic DNA exchanges in C. elegans are promoted by proximity to the synaptonemal complex.</a> Life Sci Alliance. 6 (4), e202301906(2023).</li>
<li>De Los Santos, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+mus81%2Fmms4+endonuclease+acts+independently+of+double-holliday+junction+resolution+to+promote+a+distinct+subset+of+crossovers+during+meiosis+in+budding+yeast%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">The mus81/mms4 endonuclease acts independently of double-holliday junction resolution to promote a distinct subset of crossovers during meiosis in budding yeast.</a> Genetics. 164 (1), 81-94 (2003).</li>
<li>Saito, T. T., Colaiacovo, M. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Regulation+of+crossover+frequency+and+distribution+during+meiotic+recombination%5BTitle%5D)+AND+%22Cold+Spring+Harb+Symp+Quant+Biol%22%5BJournal%5D)">Regulation of crossover frequency and distribution during meiotic recombination.</a> Cold Spring Harb Symp Quant Biol. 82, 223-234 (2017).</li>
<li>Zalevsky, J., Macqueen, A. J., Duffy, J. B., Kemphues, K. J., Villeneuve, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Crossing+over+during+Caenorhabditis+elegans+meiosis+requires+a+conserved+mutS-based+pathway+that+is+partially+dispensable+in+budding+yeast%5BTitle%5D)+AND+%22Genetics%22%5BJournal%5D)">Crossing over during Caenorhabditis elegans meiosis requires a conserved mutS-based pathway that is partially dispensable in budding yeast.</a> Genetics. 153 (3), 1271-1283 (1999).</li>
</ol>

<table><tbody><tr><td>101-1000&mu;l Pipette Tip</td><td>QSP</td><td>111-N-Q</td><td></td></tr><tr><td>0.5mol/I-EDTA Solution (pH 8.0)</td><td>Nacalai tesque</td><td>14347-21</td><td></td></tr><tr><td>10mol/I-Sodium Hydroxide Solution</td><td>Nacalai tesque</td><td>94611-45</td><td></td></tr><tr><td>10X DreamTaq Green Buffer</td><td>Thermo Fisher Scientific&nbsp;</td><td>EP0713</td><td></td></tr><tr><td>10&mu;L, Long Tip, Granduated</td><td>FUKAEKASEI, WATSON</td><td>110-207C</td><td></td></tr><tr><td>200&mu;L, Standard Tip, Graduated</td><td>FUKAEKASEI, WATSON</td><td>110-705C</td><td></td></tr><tr><td>Agar Powder</td><td>Nacalai tesque</td><td>01028-14</td><td></td></tr><tr><td>Agarose</td><td>PH Japan</td><td>PH108</td><td></td></tr><tr><td>Autoclave TOMY model ES-215 High Pressure Steam Sterilizer</td><td>TOMY SEIKO</td><td>ES-215</td><td></td></tr><tr><td>Bacto Peptone Enzymatic Digest of Protein</td><td>Thermo Fisher Scientific&nbsp;</td><td>211677</td><td></td></tr><tr><td>Boric Acid</td><td>Nacalai tesque</td><td>05215-05</td><td></td></tr><tr><td>Caenorhabditis elegans Bristol wild-type&nbsp;</td><td>Caenorhabditis Genetics Center</td><td>N2</td><td></td></tr><tr><td>Caenorhabditis elegans Hawaiian wild-type</td><td>Caenorhabditis Genetics Center</td><td>CB4856</td><td></td></tr><tr><td>Calcium Chloride</td><td>Nacalai tesque</td><td>06729-55</td><td></td></tr><tr><td>ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I</td><td>Saito lab</td><td>TTS24</td><td>originally from PD4251</td></tr><tr><td>Cholesterol</td><td>Nacalai tesque</td><td>08721-62</td><td></td></tr><tr><td>Custom TaqMan SNP Genotyping Assays</td><td>Thermo Fisher Scientific&nbsp;</td><td>4332077</td><td></td></tr><tr><td>Deoxynucleotide (dNTP) Solution Mix</td><td>New England Biolabs</td><td>N04470</td><td></td></tr><tr><td>DigPrint Doc Tablet DP-T130z</td><td>Bio Tools</td><td>DP-T130z</td><td></td></tr><tr><td>di-Potassium Hydrogenphosphate</td><td>Nacalai tesque</td><td>28726-05</td><td></td></tr><tr><td>Dissecting Microscope</td><td>Nikon</td><td>SMZ-745 C-DS</td><td></td></tr><tr><td>Dream Taq DNA Polymerase</td><td>Thermo Fisher Scientific&nbsp;</td><td>EP0713</td><td></td></tr><tr><td>Ethidium Bromide Solution</td><td>Nacalai tesque</td><td>14631-94</td><td></td></tr><tr><td>Forward Primer for WBVar01973670</td><td>IDT</td><td>103988736</td><td>atgtctcacccaaggttgaa</td></tr><tr><td>gelatin</td><td>Nacalai tesque</td><td>166-31</td><td></td></tr><tr><td>Gilson PIPETMAN Classic Pipet</td><td>Thermo Fisher Scientific&nbsp;</td><td></td><td></td></tr><tr><td>IGEPAL CA-630</td><td>Sigma-Aldrich</td><td>18896</td><td></td></tr><tr><td>Magnesium Sulfate Heptahydrate</td><td>Nacalai tesque</td><td>21003-75</td><td></td></tr><tr><td>MicroAmp Optical 96-Well Reacton Plate</td><td>Thermo Fisher Scientific&nbsp;</td><td>N8010560</td><td></td></tr><tr><td>MicroAmp Optical Adhedive Film</td><td>Thermo Fisher Scientific&nbsp;</td><td>4311971</td><td></td></tr><tr><td>MiniAmp&nbsp; thermal cycler</td><td>Thermo Fisher Scientific&nbsp;</td><td>MiniAmp</td><td></td></tr><tr><td>Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray</td><td>Bio-Rad Laboratories</td><td>1704466</td><td></td></tr><tr><td>Nichipet EXII</td><td>Nichiryo</td><td>00-NPX2-2</td><td></td></tr><tr><td>Nichipet EXII MULTI</td><td>Nichiryo</td><td>00-NPM-12VP</td><td></td></tr><tr><td>Pippete Mate NEO</td><td>Nichiryo</td><td>00-PMNEO</td><td></td></tr><tr><td>Potassium Chloride</td><td>Nacalai tesque</td><td>285-38</td><td></td></tr><tr><td>Potassium Dihydrogenphosphate</td><td>Nacalai tesque</td><td>28720-65</td><td></td></tr><tr><td>Proteinase K</td><td>Thermo Fisher Scientific&nbsp;</td><td>EO0491</td><td></td></tr><tr><td>QuantStudio 1 Real-Time PCR System, 96-well, 0.2 mL</td><td>Thermo Fisher Scientific&nbsp;</td><td>A40426</td><td></td></tr><tr><td>Quick-Load Purple 1kb Plus DNA Ladder</td><td>New England Biolabs</td><td>N0550S</td><td></td></tr><tr><td>Reverse Primer for WBVar01973670</td><td>IDT</td><td>103988737</td><td>tggctactcctagtcactga</td></tr><tr><td>Sodium Chlorids</td><td>Nacalai tesque</td><td>31319-45</td><td></td></tr><tr><td>TaqPath ProAmp Master Mix</td><td>Thermo Fisher Scientific&nbsp;</td><td>A30866</td><td></td></tr><tr><td>TC Dish 35, Standard</td><td>SARSTEDT</td><td>83.3900</td><td></td></tr><tr><td>Tris (hydroxymethyl) aminomethane</td><td>Nacalai tesque</td><td>35406-75</td><td></td></tr><tr><td>Trizma base</td><td>Sigma-Aldrich</td><td>&nbsp;T1503-1KG</td><td></td></tr><tr><td>Tween 20</td><td>Yoneyama Reagent</td><td>03706</td><td></td></tr><tr><td>WormStuff Worm Pick</td><td>Genesee Scientific</td><td>59-AWP</td><td></td></tr><tr><td>WormStuff Worm Pick Tips(90/10% Pt/Ir, 30 Gauge)</td><td>Genesee Scientific</td><td>59-30PI36</td><td></td></tr></tbody></table>

frequentie en distributie van cross-overs in caenorhabditis elegans meiose door snp-genotypering met behulp van real-time pcr

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Frequentie en distributie van cross-overs in Caenorhabditis elegans Meiose door SNP-genotypering met behulp van real-time PCR

Caenorhabditis elegans is an excellent model organism for studying meiosis. In addition to general advantages, such as a short reproductive cycle, many ...

frequency-distribution-crossovers-caenorhabditis-elegans-meiosis-snp

Research

JoVE Journal

Genetics

Genetica

Deze video beschrijft een techniek om een enkel nematode snel te screenen op een genetische marker door PCR-screening. In het voorbeeldprotocol screenen we op CRISPR-gebaseerde genoombewerking.

Single Worm PCR: een methode om genomisch DNA te extraheren en te versterken

Dit protocol beschrijft een snelle en materiaalbesparende procedure voor de integratie van transgene extrachromosomale arrays in het Caenorhabditis elegans genoom met behulp van ultraviolette (UV) bestraling. Bovendien is dit protocol bijzonder geschikt voor transgene lijnen die extrachromosomale arrays met een hoge snelheid overdragen.

Een snelle protocol voor de integratie Extrachromosomaal Arrays Met High Transmission Rate in de<em&gt; C. elegans</em&gt; Genome

Genetisch gecodeerde histon-miniSOG induceert genoom-brede erfelijke mutaties in een blauw licht-afhankelijke manier. Dit mutagenese methode is eenvoudig, snel, vrij van giftige chemicaliën, en zeer geschikt voor forward genetische screening en transgene integratie.

Optogenetische willekeurige mutagenese met behulp van histon-miniSOG in<em&gt; C. elegans</em

Deze methode zorgt voor de generatie van tetraploïde en Triploïde Caenorhabditis nematoden van een diploïde stam. Polyploïde stammen die zijn gegenereerd door deze methode zijn gebruikt voor het bestuderen van chromosoom interacties in Meiotische profase, en deze methode is nuttig voor de behandeling van belangrijke fundamentele vragen in de cel, ontwikkelingsstoornissen, evolutionaire, en Kankerbiologie.

Manipulatie van ploïdie in Caenorhabditis elegans

Hier presenteren we een methode om het genoom van C. elegans ingenieur met behulp van CRISPR-Cas9 ribonucleoproteins en homologie afhankelijke reparatie sjablonen.

Een snelle en Facile pijpleiding voor het genereren van Genomic punt mutanten in C. elegans met behulp van CRISPR/Cas9 Ribonucleoproteins

Hier presenteren we een gedetailleerd protocol voor het identificeren van homologe recombinatie gebeurtenissen die hebben plaatsgevonden in muis embryonale stamcellen met behulp van Southern blotting en/of PCR. Deze methode wordt geïllustreerd door de generatie van nonmuscle myosin II genetische vervanging Muismodellen met behulp van traditionele embryonale stamcel gebaseerde homologe recombinatie-gemedieerde targeting technologie.

Identificatie van homologe recombinatie gebeurtenissen in muis embryonale stamcellen met behulp van Southern Blotting en de Kettingreactie van de Polymerase

In dit artikel wordt een protocol met hoge doorvoer beschreven voor een snelle en betrouwbare bepaling van de genexpressieniveaus in enkele of bulk C. elegansmonsters. Dit protocol vereist geen RNA-isolatie en produceert cDNA rechtstreeks uit monsters. Het kan worden gebruikt samen met high-throughput multiplexed nanofluidic real-time qPCR platforms.

Kwantitatieve RT-PCR met hoge doorvoer in enkele en bulk C. elegans-monsters met behulp van nanofluïdische technologie

Dit protocol kan worden gebruikt om grootschalige ecologische onderzoeken uit te voeren van zelfrijdende Caenorhabditis-nematoden . Het belangrijkste voordeel van deze methode is de efficiënte organisatie en analyse van ecologische en moleculaire gegevens die verband houden met de nematoden die uit de natuur zijn verzameld.

Een zeer schaalbare aanpak om ecologische onderzoeken van zelfrijdende Caenorhabditis-nematoden uit te voeren

Er wordt een protocol beschreven dat lasermicrodissectie gebruikt om individuele nematodenweefsels te isoleren voor RNA-sequencing. Het protocol vereist geen soortspecifieke genetische toolkits, waardoor genexpressieprofielen kunnen worden vergeleken tussen verschillende soorten op het niveau van single-tissue monsters.

Laser microdissectie voor species-agnostische single-tissue toepassingen

Dit is een veelgebruikte methode voor C. elegans gonad-dissectie gevolgd door vriesscheur, die kiembaanmonsters produceert voor immunofluorescentie via antilichaamkleuring, of voor eenvoudige DAPI-kleuring om DNA te visualiseren. Dit protocol is succesvol geweest voor studenten in een onderzoekslaboratorium en in een op cursussen gebaseerde niet-gegradueerde onderzoekservaring.

C. elegans Gonad dissectie en freeze crack voor immunofluorescentie en DAPI-kleuring

Hier presenteren we een snel en eenvoudig ATAC-seq-protocol dat geïmplementeerd kan worden in L4-fase Caenorhabditis elegans whole-worm met slechts 30 μL wormpellet.

Chromatine toegankelijkheidsprofilering bij hele Caenorhabditis elegans L4-larven

Leer SNP-genotypering met real-time PCR om crossoverfrequentie en -distributie te meten tijdens meiose in Caenorhabditis elegans. Deze methode verbetert de nauwkeurigheid van genetisch onderzoek.

Frequentie en distributie van cross-overs in Caenorhabditis elegans Meiose door SNP-genotypering met behulp van real-time PCR

Meer video's verkennen