Name: In vivo Beeldvorming van neurale activiteit bij onverdoofde Drosophila-volwassen vliegen (Video) | JoVE Journal
Uploaded: 2025-06-20T13:30:00.000+00:00
Duration: 9 min 15 s
Description: Leer in vivo beeldvorming van neurale activiteit bij onverdoofde volwassen Drosophila-vliegen. Deze methode onthult hoe specifieke dopaminerge neuronen geheugenvergeten reguleren en cognitieve flexibiliteit ondersteunen.

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</ol>

<table><tbody><tr><td>0.102 mm Platinumdraad</td><td>Surepure Chemetals</td><td>2690</td><td></td></tr><tr><td>0.25 OD, 0.125 ID, platina-geharde siliconen slang</td><td>VWR</td><td>89068-474</td><td></td></tr><tr><td>0.3 mm ID Siliconen slang&nbsp;</td><td>Fisher Scientific</td><td>11-189-14</td><td></td></tr><tr><td>Banaanstekkers</td><td>Amazon</td><td></td><td></td></tr><tr><td>Zwarte acrylplaat voor kamer 0,0125 inch</td><td>Precision Brand</td><td>44250</td><td></td></tr><tr><td>Calcium Chloride</td><td>Sigma-Aldrich</td><td>746495</td><td></td></tr><tr><td>Koperen schokraster</td><td>Op maat gemaakt</td><td></td><td></td></tr><tr><td>DC voeding voor platina weerstand</td><td>Fisher Scientific</td><td>S35740</td><td></td></tr><tr><td>Dremel gereedschap 3000-1</td><td>Dremel</td><td>3000-N/18</td><td></td></tr><tr><td>Drosophila melanogaster / ;;uas-tdtom</td><td>BDSC</td><td>36328</td><td></td></tr><tr><td>Drosophila melanogaster / ;uas-gcamp6f;</td><td>BDSC</td><td>42747</td><td>Deze uas-lijn werd gecombineerd met ;;uas-td-tom om een stock te genereren die beide inserts bevat</td></tr><tr><td>Drosophila melanogaster/ ;;MB077C-gal4</td><td>BDSC</td><td>68287</td><td></td></tr><tr><td>Epoxylijm&nbsp;</td><td>Gorilla</td><td>4200102</td><td></td></tr><tr><td>Gas flowmeter 1,5 LPM</td><td>Amazon</td><td>B01N0UWZ2T</td><td></td></tr><tr><td>Glas 1 mL serologische pipette&nbsp;</td><td>Fisher Scientific</td><td>50-232-9516</td><td></td></tr><tr><td>Glijplaten</td><td>Fisher Scientific&nbsp;</td><td>12-550-A3</td><td></td></tr><tr><td>Glucose</td><td>Sigma-Aldrich</td><td>G7528</td><td></td></tr><tr><td>HEPES</td><td>Sigma-Aldrich</td><td>H4034</td><td></td></tr><tr><td>Hypodermisch 12TW gauge slang</td><td>Microgroup</td><td>316H12TW</td><td></td></tr><tr><td>Hypodermisch 22X gauge slang</td><td>Microgroup</td><td>304H22X</td><td></td></tr><tr><td>Magnesium Chloride Hexahydraat</td><td>Sigma-Aldrich</td><td>M9272</td><td></td></tr><tr><td>Myristinezuur</td><td>Acros Organics</td><td>156961000</td><td></td></tr><tr><td>Neodymium magneten 3X2 mm</td><td>Amazon</td><td>B0CXSQ387M</td><td></td></tr><tr><td>P1000 tips</td><td>Genesee Scientific&nbsp;</td><td>23-165RL</td><td></td></tr><tr><td>P200 tips</td><td>Genesee Scientific&nbsp;</td><td>23-150RL</td><td></td></tr><tr><td>Pneumatisch push-connector 6mm OD</td><td>Amazon</td><td>B07ZHG8Y1Y</td><td></td></tr><tr><td>Kalium Chloride</td><td>Sigma-Aldrich</td><td>P3911</td><td></td></tr><tr><td>PTFE slang 6mm OD</td><td>Amazon</td><td></td><td></td></tr><tr><td>Veiligheidsmesjes&nbsp;</td><td>Amazon</td><td>B08LYD6645</td><td></td></tr><tr><td>Natrium Bicarbonaat</td><td>Sigma-Aldrich</td><td>S5761</td><td></td></tr><tr><td>Natrium Chloride</td><td>Fisher Scientific</td><td>S271-500</td><td></td></tr><tr><td>Natriumfosfaat dibasisch</td><td>Sigma-Aldrich</td><td>S0876</td><td></td></tr><tr><td>Sucrose</td><td>Sigma-Aldrich</td><td>S9378</td><td></td></tr><tr><td>Trehalose</td><td>Sigma-Aldrich</td><td>T0167</td><td></td></tr></tbody></table>

in vivo beeldvorming van neurale activiteit bij onverdoofde drosophila-volwassen vliegen

In Vivo Imaging of Neural Activity in Unanesthetized Drosophila Adult Flies

In vivo Beeldvorming van neurale activiteit bij onverdoofde Drosophila-volwassen vliegen

In vivo imaging is crucial for studying neurobiology in Drosophila as it allows real-time visualization of neuronal activity, development, and plasticity ...

in-vivo-imaging-neural-activity-unanesthetized-drosophila-adult

Research

JoVE Journal

Neuroscience

Neurowetenschap

Dit protocol beschrijft een betrouwbare methode voor verdoving en beeldvorming van...

In vivo beeldvorming van Intact Drosophila larven aan Subcellulaire resolutie

Deze video beschrijft in vivo calcium beeldvorming in Drosophila neuronen met behulp van GCaMP. De aanbevolen protocolclip laat zien hoe u veranderingen in GCaMP-fluorescentie van paddestoellichaamneuronen kunt registreren tijdens een olfactorisch conditioneringsexperiment.

Drosophila In Vivo Calcium imaging: een methode voor functionele beeldvorming van neuronale activiteit

This video demonstrates a method for in vivo calcium imaging using synaptically localized calcium sensors in flies. It outlines the steps for preparing the flies for microscopy, conducting olfactory conditioning, and visualizing odor-induced calcium influx.

In Vivo Calcium Imaging Using Synaptically Localized Calcium Sensors in Drosophila melanogaster

Dit protocol Gegevens een gestroomlijnde methode voor live cell imaging uitvoeren in het kader van een intact larvale hersenen. Live cell imaging benaderingen zijn van onschatbare waarde voor de studie van asymmetrische neurale stamcellen divisies evenals andere neurogene en ontwikkelingsprocessen, consequent blootleggen van mechanismen die eerder over het hoofd gezien.

Live-Imaging van<em&gt; Drosophila</em&gt; Larvale neuroblasts

Hier presenteren we een nieuwe Ca 2+ -Imaging aanpak met behulp van een bioluminescente reporter. Deze benadering maakt gebruik van een gefuseerde construct GFP-aequorine dat bindt aan Ca2 + en licht emitteert, waardoor de noodzaak voor excitatie licht. Aanzienlijk deze methode maakt het mogelijk lange doorlopende beeldvorming, de toegang tot diepe hersenstructuren en hoge tijdsresolutie.

In vivo Functional Brain Imaging aanpak op basis van Bioluminescent Calcium Indicator GFP-aequorine

Hier we illustreren hoe enkel molecuul foto-geactiveerde lokalisatie microscopie kan worden uitgevoerd op de motorische zenuw-terminal van een levende Drosophila melanogaster derde instar larve.

In Vivo Single-molecuul volgen bij de Terminal van de presynaptische motorische zenuw Drosophila

Dit witboek beschrijft een protocol voor ex vivo calcium beeldvorming van de hersenen van Drosophila . Bij deze methode, kunnen natuurlijke of synthetische verbindingen worden toegepast op de buffer voor het testen van hun vermogen om bepaalde neuronen in de hersenen activeren.

Ex Vivo Calcium Imaging voor het visualiseren van hersenen reacties op endocriene signalering in Drosophila

Hier presenteren we een protocol waarmee pre-en/of postsynaptisch calcium kan worden gevisualiseerd in de context van Drosophila leren en geheugen. In vivo calcium beeldvorming met behulp van Synaptisch gelokaliseerde calcium sensoren wordt gecombineerd met een klassieke olfactorische conditionerings paradigma zodanig dat de Synaptische plasticiteit onderliggende dit type associatief leren kan worden bepaald.

In vivo optische calcium beeldvorming van leren-geïnduceerde synaptische plasticiteit in Drosophila melanogaster

We presenteren een methode die specifiek is afgestemd op het beeld van de hele hersenen van volwassen Drosophila tijdens gedrag en als reactie op stimuli. Het hoofd is gepositioneerd om optische toegang tot de hele hersenen mogelijk te maken, terwijl de vlieg zijn benen en de antennes kan bewegen, de punt van de proboscis en de ogen zintuiglijke stimuli kunnen ontvangen.

Volwassen Drosophila melanogaster voorbereiden op whole brain imaging tijdens gedrags- en stimulireacties

Hier presenteren we een protocol voor ex vivo calciumbeeldvorming bij volwassen Drosophila met GCaMP6-expressie om epileptiforme activiteiten te monitoren. Het protocol biedt een waardevol hulpmiddel voor het onderzoeken van ictale gebeurtenissen bij volwassen Drosophila door middel van ex vivo calciumbeeldvorming, waardoor de mogelijke mechanismen van epilepsie op cellulair niveau kunnen worden onderzocht.

Ex Vivo Calciumbeeldvorming voor Drosophila-model van epilepsie

We presenteren calciumbeeldvormingsprotocollen voor een Drosophila-larvale olfactorische neuron, met behulp van een topische weefsellijm voor immobilisatie. Deze methode verbetert de stabiliteit en maakt betrouwbare in- en ex-vivo-experimenten mogelijk. Aangepaste R-scripts analyseren calciumsignalen en bieden een efficiënt platform voor gedetailleerd neurofysiologisch onderzoek.

In-vivo en ex-vivo calciumbeeldvorming van een reukcircuitneuron in de larve van de Drosophila melanogaster met een derde instar

Leer in vivo beeldvorming van neurale activiteit bij onverdoofde volwassen Drosophila-vliegen. Deze methode onthult hoe specifieke dopaminerge neuronen geheugenvergeten reguleren en cognitieve flexibiliteit ondersteunen.

In vivo Beeldvorming van neurale activiteit bij onverdoofde Drosophila-volwassen vliegen

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