Name: Amplicon Sequencing met behulp van de Long-Read Sequencing-technologieën (Video) | JoVE Journal
Uploaded: 2025-08-29T13:40:00.000+00:00
Duration: 8 min 57 s
Description: Leer amplicon-sequencing met long-read technologieën voor tuberculose-diagnostiek. Deze methode biedt snellere resultaten en vereenvoudigde workflows voor resistentiebepaling in klinische samples.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Tuberculosis report. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Baltimore+Health+News%22">Baltimore Health News</a>. , WHO. (2022).</li>
<li>WHO Malaria Department report. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Report+20-23%22">Report 20-23</a>. , WHO. (2023).</li>
<li><a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22The+End+Strategy+TB%22">The End Strategy TB</a>. 53 (1), WHO. 1689-1699 (2015).</li>
<li>Cabibbe, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Application+of+targeted+nextgeneration+sequencing+assay+on+a+portable+sequencing+platform+for+culturefree+detection+of+drugresistant+tuberculosis+from+clinical+samples%5BTitle%5D)+AND+%22J+Clin+Microbiol%22%5BJournal%5D)">Application of targeted nextgeneration sequencing assay on a portable sequencing platform for culturefree detection of drugresistant tuberculosis from clinical samples.</a> J Clin Microbiol. 58 (10), e0063220(2020).</li>
<li>Jouet, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deep+amplicon+sequencing+for+culturefree+prediction+of+susceptibility+or+resistance+to+13+antituberculous+drugs%5BTitle%5D)+AND+%22Eur+Respir+J%22%5BJournal%5D)">Deep amplicon sequencing for culturefree prediction of susceptibility or resistance to 13 antituberculous drugs.</a> Eur Respir J. 57 (3), 2002338(2021).</li>
<li>NG, K. C. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((How+well+do+routine+molecular+diagnostics+detect+Rifampin%5BTitle%5D)+AND+%22J+Clin+Microbiol%22%5BJournal%5D)">How well do routine molecular diagnostics detect Rifampin.</a> J Clin Microbiol. 57 (1), e0128723(2019).</li>
<li>MacLean, E., Kohli, M., Weber, S. F., Suresh, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advances+in+molecular+diagnosis+of+tuberculosis%5BTitle%5D)+AND+%22J+Clin+Microbiol%22%5BJournal%5D)">Advances in molecular diagnosis of tuberculosis.</a> J Clin Microbiol. 58 (10), e0158219(2020).</li>
<li>Sibandze, D. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+molecular+diagnostics+of+tuberculosis+resistance+by+targeted+stool+sequencing%5BTitle%5D)+AND+%22Genome+Med%22%5BJournal%5D)">Rapid molecular diagnostics of tuberculosis resistance by targeted stool sequencing.</a> Genome Med. 14 (1), 42(2022).</li>
<li>Shah, M., Chihota, V., Coetzee, G., Churchyard, G., Dorman, S. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+laboratory+costs+of+rapid+molecular+tests+and+conventional+diagnostics+for+detection+of+tuberculosis+and+drugresistant+tuberculosis+in+South+Africa%5BTitle%5D)+AND+%22BMC+Infect.+Dis%22%5BJournal%5D)">Comparison of laboratory costs of rapid molecular tests and conventional diagnostics for detection of tuberculosis and drugresistant tuberculosis in South Africa.</a> BMC Infect. Dis. 13 (1), 352(2013).</li>
<li>Hu, T., Chitnis, N., Monos, D., Dinh, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nextgeneration+sequencing+technologies%3A+An+overview%5BTitle%5D)+AND+%22Hum+Immunol%22%5BJournal%5D)">Nextgeneration sequencing technologies: An overview.</a> Hum Immunol. 82 (10), 801-811 (2021).</li>
<li>Pervez, M. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+comprehensive+review+of+performance+of+nextgeneration+sequencing+platforms%5BTitle%5D)+AND+%22Biomed+Res+Int%22%5BJournal%5D)">A comprehensive review of performance of nextgeneration sequencing platforms.</a> Biomed Res Int. 2022, 3457806(2022).</li>
<li>Tafess, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targetedsequencing+workflows+for+comprehensive+drug+resistance+profiling+of+Mycobacterium+tuberculosis+cultures+using+two+commercial+sequencing+platforms%3A+Comparison+of+analytical+and+diagnostic+performance%2C+turnaround+time%2C+and+cost%5BTitle%5D)+AND+%22Clin+Chem%22%5BJournal%5D)">Targetedsequencing workflows for comprehensive drug resistance profiling of Mycobacterium tuberculosis cultures using two commercial sequencing platforms: Comparison of analytical and diagnostic performance, turnaround time, and cost.</a> Clin Chem. 66 (6), 809-820 (2020).</li>
<li>Use of targeted nextgeneration sequencing to detect drugresistant tuberculosis. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22WHO+rapid+communication%22">WHO rapid communication</a>. , WHO. https://www.who.int/publications/i/item/9789240076372 (2023).</li>
<li>Hazra, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+wholegenome+sequencing+of+Mycobacterium+tuberculosis+on+treatment+outcomes+for+MDRTB%2FXDRTB%3A+A+systematic+review%5BTitle%5D)+AND+%22Pharmaceutics%22%5BJournal%5D)">Impact of wholegenome sequencing of Mycobacterium tuberculosis on treatment outcomes for MDRTB/XDRTB: A systematic review.</a> Pharmaceutics. 15 (6), 1408(2023).</li>
<li>Shea, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comprehensive+wholegenome+sequencing+and+reporting+of+drug+resistance+profiles+on+clinical+cases+of+Mycobacterium+tuberculosis+in+New+York+State%5BTitle%5D)+AND+%22J+Clin+Microbiol%22%5BJournal%5D)">Comprehensive wholegenome sequencing and reporting of drug resistance profiles on clinical cases of Mycobacterium tuberculosis in New York State.</a> J Clin Microbiol. 55 (6), 1871-1882 (2017).</li>
<li>Meehan, C. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Whole+genome+sequencing+of+Mycobacterium+tuberculosis%3A+Current+standards+and+open+issues%5BTitle%5D)+AND+%22Nat+Rev+Microbiol%22%5BJournal%5D)">Whole genome sequencing of Mycobacterium tuberculosis: Current standards and open issues.</a> Nat Rev Microbiol. 17 (10), 533-545 (2019).</li>
<li>Heupink, T. H., Verboven, L., Warren, R. M., Van Rie, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comprehensive+and+accurate+genetic+variant+identification+from+contaminated+and+lowcoverage+Mycobacterium+tuberculosis+whole+genome+sequencing+data%5BTitle%5D)+AND+%22Microb+Genom%22%5BJournal%5D)">Comprehensive and accurate genetic variant identification from contaminated and lowcoverage Mycobacterium tuberculosis whole genome sequencing data.</a> Microb Genom. 7 (1), e000555(2021).</li>
<li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Interpretation+of+the+third+edition+of+WHO+consolidated+guidelines+on+tuberculosis%3A+Module+3%3A+Diagnosis%3A+Rapid+diagnostics+for+tuberculosis+detection%5BTitle%5D)+AND+%22Chin+J+Antituberc%22%5BJournal%5D)">Interpretation of the third edition of WHO consolidated guidelines on tuberculosis: Module 3: Diagnosis: Rapid diagnostics for tuberculosis detection.</a> Chin J Antituberc. 46 (2), 120-130 (2024).</li>
<li>Kambli, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeted+nextgeneration+sequencing+directly+from+sputum+for+comprehensive+genetic+information+on+drugresistant+Mycobacterium+tuberculosis%5BTitle%5D)+AND+%22Tuberculosis%22%5BJournal%5D)">Targeted nextgeneration sequencing directly from sputum for comprehensive genetic information on drugresistant Mycobacterium tuberculosis.</a> Tuberculosis. 127 (1), 102051(2021).</li>
<li>Use of targeted nextgeneration sequencing to detect drugresistant tuberculosis. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Rapid+Communication%22">Rapid Communication</a>. , WHO. 1-8 (2023).</li>
<li>Nandlal, L., Perumal, R., Naidoo, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+molecular+assays+for+the+diagnosis+of+drugresistant+tuberculosis%5BTitle%5D)+AND+%22Infect+Drug+Resist%22%5BJournal%5D)">Rapid molecular assays for the diagnosis of drugresistant tuberculosis.</a> Infect Drug Resist. 15 (1), 4971-4984 (2022).</li>
<li>Dippenaar, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nanopore+sequencing+for+Mycobacterium+tuberculosis%3A+A+critical+review+of+the+literature%2C+new+developments%2C+and+future+opportunities%5BTitle%5D)+AND+%22J+Clin+Microbiol%22%5BJournal%5D)">Nanopore sequencing for Mycobacterium tuberculosis: A critical review of the literature, new developments, and future opportunities.</a> J Clin Microbiol. 60 (5), e0123422(2022).</li>
<li>Chan, W. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+economical+drug+resistance+profiling+with+nanopore+MinION+for+clinical+specimens+with+low+bacillary+burden+of+Mycobacterium+tuberculosis%5BTitle%5D)+AND+%22BMC+Res+Notes%22%5BJournal%5D)">Rapid and economical drug resistance profiling with nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis.</a> BMC Res Notes. 13 (1), 123(2020).</li>
<li>Feuerriegel, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+genomic+first+and+secondline+drug+resistance+prediction+from+clinical+Mycobacterium+tuberculosis+specimens+using+DeeplexMycTB%5BTitle%5D)+AND+%22Eur+Respir+J%22%5BJournal%5D)">Rapid genomic first and secondline drug resistance prediction from clinical Mycobacterium tuberculosis specimens using DeeplexMycTB.</a> Eur Respir J. 57 (4), 2004784(2021).</li>
<li>GenoScreen product brochure. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Deeplex+MycTB%22">Deeplex MycTB</a>. , GenoScreen. (2023).</li>
<li>Mtafya, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+practical+approach+to+render+tuberculosis+samples+safe+for+application+of+tuberculosis+molecular+bacterial+load+assay+in+clinical+settings+without+a+biosafety+level+3+laboratory%5BTitle%5D)+AND+%22Tuberculosis%22%5BJournal%5D)">A practical approach to render tuberculosis samples safe for application of tuberculosis molecular bacterial load assay in clinical settings without a biosafety level 3 laboratory.</a> Tuberculosis. 138 (1), 102194(2023).</li>
<li>Suganthi, P., Usharani, B., Venkateswari, R., Muthuraj, M. Biosafety practices in mycobacteriology laboratory. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSuganthi%2C+P+%22Research+Advances+in+Microbiology+and+Biotechnology%22">Research Advances in Microbiology and Biotechnology</a>. 3, B P International (a part of SCIENCEDOMAIN International). Chennai. 1-39 (2023).</li>
<li>consolidated guidelines on tuberculosis. Module 4: Treatment and care. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22WHO+guideline%22">WHO guideline</a>. , WHO. (2022).</li>
<li>Williams, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Matrixbased+DNA+extraction+for+targeted+nextgeneration+sequencing+on+decontaminated+sputum+samples%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Matrixbased DNA extraction for targeted nextgeneration sequencing on decontaminated sputum samples.</a> J Vis Exp. (181), e68147(2025).</li>
<li>Delahaye, C., Nicolas, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sequencing+DNA+with+nanopores%3A+Troubles+and+biases%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Sequencing DNA with nanopores: Troubles and biases.</a> PLoS One. 16 (2), e0245534(2021).</li>
<li>Zhao, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+identification+of+drugresistant+tuberculosis+genes+using+direct+PCR+amplification+and+Oxford+Nanopore+Technology+sequencing%5BTitle%5D)+AND+%22Can+J+Infect+Dis+Med+Microbiol%22%5BJournal%5D)">Rapid identification of drugresistant tuberculosis genes using direct PCR amplification and Oxford Nanopore Technology sequencing.</a> Can J Infect Dis Med Microbiol. 2022, 8845672(2022).</li>
<li>Tagliani, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Culture+and+nextgeneration+sequencingbased+drug+susceptibility+testing+unveil+high+levels+of+drugresistantTB+in+Djibouti%3A+Results+from+the+first+national+survey%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Culture and nextgeneration sequencingbased drug susceptibility testing unveil high levels of drugresistantTB in Djibouti: Results from the first national survey.</a> Sci Rep. 7 (1), 15076(2017).</li>
<li>Smith, C., Halse, T. A., Shea, J., Modestil, H., Fowler, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+nanopore+sequencing+for+clinical+diagnostics%3A+For+Mycobacterium+tuberculosis%5BTitle%5D)+AND+%22J+Clin+Microbiol%22%5BJournal%5D)">Assessing nanopore sequencing for clinical diagnostics: For Mycobacterium tuberculosis.</a> J Clin Microbiol. 59 (7), e0123421(2021).</li>
</ol>

<table><tbody><tr><td>15 mL &amp; 1.5 mL or 2 mL Lo-binding Tubes</td><td>Eppendorf</td><td>EP0030108051-250EA</td><td></td></tr><tr><td>1kb ladder</td><td>Thermo Fisher Scientific</td><td>SM0311</td><td></td></tr><tr><td>2 mL screw cap Tubes</td><td>Scientific Specialties Inc</td><td>P2TUB056C-0001.5ST</td><td></td></tr><tr><td>2 mm sterile glass beads</td><td>Sigma-Aldrich</td><td>Z273627-1EA</td><td></td></tr><tr><td>AMPure XP Bead</td><td>Beckman Colter</td><td>A63881</td><td></td></tr><tr><td>Deeplex Myc-TB&nbsp;</td><td>GenoScreen</td><td>20090205</td><td></td></tr><tr><td>Electrophoresis chamber</td><td>Thermo Fisher Scientific</td><td>A25977</td><td></td></tr><tr><td>Ethyl alcohol, Pure</td><td>Sigma-Aldrich</td><td>E7023-500ML</td><td></td></tr><tr><td>FastPrep Homogenizer</td><td>MP Biomedicals</td><td>116005500</td><td></td></tr><tr><td>Filter pipette tips (10 &micro;L)&nbsp;</td><td>Bio-Smart Scientific</td><td>FT-10-R</td><td></td></tr><tr><td>Filter pipette tips (1000 &micro;L)&nbsp;</td><td>Bio-Smart Scientific</td><td>FT-1000-R</td><td></td></tr><tr><td>Filter pipette tips (20 &micro;L)&nbsp;</td><td>Bio-Smart Scientific</td><td>FT-20-R</td><td></td></tr><tr><td>Filter pipette tips (200 &micro;L)&nbsp;</td><td>Bio-Smart Scientific</td><td>FT-200-R</td><td></td></tr><tr><td>Flow-cell&nbsp;</td><td>Oxford Nanopore Technologies</td><td>FLO-MIN114</td><td></td></tr><tr><td>Heating-block</td><td>Eppendorf</td><td>5382000031</td><td></td></tr><tr><td>InstaGene Matrix</td><td>Bio-Rad</td><td>BBRD7326030</td><td></td></tr><tr><td>Magnetic Rack&nbsp;</td><td>Thermo Fisher Scientific&nbsp;</td><td>12321D</td><td></td></tr><tr><td>Microcentrifuge</td><td>Eppendorf&nbsp;</td><td>5406000046</td><td></td></tr><tr><td>MinION</td><td>Oxford Nanopore Technologies</td><td>MK1B</td><td></td></tr><tr><td>Native-barcoding kit V14&nbsp;</td><td>Oxford Nanopore Technologies</td><td>SQK-NBD114.24</td><td></td></tr><tr><td>NEB Blunt/TA Ligase Master Mix</td><td>NEB</td><td>M0367</td><td></td></tr><tr><td>NEBNext Quick Ligation Module</td><td>NEB</td><td>E6056</td><td></td></tr><tr><td>NEBNext Ultra II End repair/dA-tailing Module</td><td>NEB</td><td>E7546</td><td></td></tr><tr><td>Nuclease-free water (NFW)</td><td>Thermo Fisher Scientific</td><td>AM9937</td><td></td></tr><tr><td>Pipettes (P10, P20, P200 and P1000)</td><td>Eppendorf</td><td>EP3123000918-1EA</td><td></td></tr><tr><td>Qubit Assay Tubes</td><td>Thermo Fisher Scientific</td><td>Q32856</td><td></td></tr><tr><td>Qubit dsDNA HS Assay Kit</td><td>Thermo Fisher Scientific</td><td>Q33231</td><td></td></tr><tr><td>Qubit fluorometer</td><td>Thermo Fisher Scientific</td><td>Q33226</td><td></td></tr><tr><td>SYBR Safe DNA gel Strain</td><td>Thermo Fisher Scientific</td><td>S33102</td><td></td></tr><tr><td>UltraPure Agarose</td><td>Thermo Fisher Scientific</td><td>17852</td><td></td></tr><tr><td>Universal Hood III transilluminator (Molecular Imager Gel Doc XR System)</td><td>Bio-Rad</td><td>170-8170</td><td></td></tr><tr><td>Vortex mixer</td><td>Lasec</td><td>WMBB0L0E0216</td><td></td></tr></tbody></table>

amplicon sequencing met behulp van de long-read sequencing-technologieën

Amplicon Sequencing using the Long-Read Sequencing Technologies

Amplicon Sequencing met behulp van de Long-Read Sequencing-technologieën

The World Health Organization (WHO) continues to emphasize the urgent need for a rapid, cost-effective, and user-friendly diagnostic method for ...

amplicon-sequencing-using-the-long-read-sequencing-technologies

Research

JoVE Journal

Biochemistry

Biochemie

Hier beschrijven we een methode voor de bereiding van zowel de single lezen en gekoppeld end Illumina mRNA-Seq sequencing bibliotheken voor genexpressie analyse op basis van T7-RNA lineaire versterking. Dit protocol vereist slechts 10 nanogram van het starten van totaal RNA en genereert een uiterst consistente bibliotheken vertegenwoordigt hele transcripten.

Single Lees en Gepaarde End mRNA-Seq Illumina Bibliotheken van 10 nanogram Totaal RNA

Het karakteriseren van microbiële gemeenschappen is een al lang bestaand doel in de milieumicrobiologie. Methoden voor next-generation sequencing maken nu de karakterisering van microbiële gemeenschappen mogelijk op een ongekende diepte met minimale kosten en arbeid. We beschrijven hier onze aanpak om bacteriële 16S ribosomale RNA-genen te sequencen met behulp van een tafelsequencer.

Next-generation sequencing van het 16S ribosomaal RNA Gene Amplicons

Dit protocol beschrijft een methode voor de toewijzing pre-mRNA 3' eind verwerking van sites.

3' eind Sequencing bibliotheek voorbereiding met A-seq2

Full-length individuele proviraal sequencing (FLIPS) een efficiënte en hoog-productie methode voorziet in de versterking en sequentiebepaling van één, in de buurt van full-length (intact en defecte) HIV-1 proviruses en zorgt voor de bepaling van hun potentieel replicatie-competentie. FLIPS overwint beperkingen van eerdere tests ontworpen om de volgorde van het latente reservoir van HIV-1.

Versterking van in de buurt van Full-length HIV-1 Proviruses voor Next-Generation Sequencing

Lange-lezen sequenties vergemakkelijken aanzienlijk de assemblage van complexe genomen en karakterisering van structurele variatie. Beschrijven we een methode voor het genereren van ultra lange reeksen door sequentiebepaling nanopore gebaseerde platformen. De benadering neemt een geoptimaliseerde DNA-extractie, gevolgd door de gewijzigde bibliotheek preparaten voor het genereren van honderden kilobase leest met gematigde dekking van menselijke cellen.

Ultra lange lezen Sequencing voor hele Genomic DNA-analyse

Nanogaatje sequencing is een nieuwe technologie die het mogelijk maakt kosteneffectieve sequencing in afgelegen locaties en resource-arme instellingen. Hier, presenteren wij een protocol voor het rangschikken van mRNAs van geheel bloed dat met dergelijke voorwaarden compatibel is.

Het rangschikken van mRNA van geheel bloed gebruikend Nanogaatje het rangschikken

Dit protocol beschrijft een workflow van ex vivo of in vitro celculturen tot transcriptomische gegevensvoorverwerking voor kosteneffectieve transcriptoomgebaseerde geneesmiddelenscreening.

Kostenefficiënte transcriptomische screening van geneesmiddelen

De huidige studie beschrijft een workflow voor nanoporesequencing van plasmiden wanneer de chromosomale sequentie al bekend is of niet van belang is. Een initiële plasmide-DNA-verrijkingsstap zorgt voor een optimale plasmidesequentiediepte en maximaliseert het aantal monsters dat per flowcel kan worden gesequenced.

Long-read plasmidvolgordebepaling en assemblage met behulp van op nanopore sequencing gebaseerde workflows

De huidige studie beschrijft een eenvoudige methode voor het opsporen van endogene niveaus van Rab10 fosforylering door leucine-rijke herhalen kinase 2.

Rab10 fosforylering detectie door LRRK2 activiteit met behulp van SDS-pagina met een fosfaat-bindende-Tag

Een van de meest uitdagende stress voorwaarden die organismen tijdens hun leven tegenkomen gaat de accumulatie van oxidanten. Tijdens oxidatieve stress afhankelijk cellen sterk zijn van moleculaire chaperones. Hier presenteren we methoden voor het onderzoeken van de redox-gereglementeerde anti-aggregatie activiteit, evenals het monitoren van de structurele veranderingen in het bestuur van de Chaperon-functie met behulp van HDX-MS.

Hsp33 de Redox-gereglementeerde Chaperone activiteit definiëren en in kaart brengen van de wijzigingen van de conformationele op Hsp33 met behulp van spectrometrie van de massa van de Exchange waterstof-deuterium

Leer amplicon-sequencing met long-read technologieën voor tuberculose-diagnostiek. Deze methode biedt snellere resultaten en vereenvoudigde workflows voor resistentiebepaling in klinische samples.

Amplicon Sequencing met behulp van de Long-Read Sequencing-technologieën

Meer video's verkennen