Name: Milieubemonstering van fotosynthetische microben en hun virussen: van veld tot laboratorium (Video) | JoVE Journal
Uploaded: 2025-07-03T14:30:00.000+00:00
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Description: Ontdek methoden voor milieubemonstering van fotosynthetische microben en hun virussen met behulp van elektronenmicroscopie, kwantitatieve PCR en next-generation sequencing. Deze technieken helpen onderzoekers virus-gastheer dynamiek te karakteriseren en voorspellende modellen te ontwikkelen.

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<li>Douterelo, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Methodological+approaches+for+studying+the+microbial+ecology+of+drinking+water+distribution+systems%5BTitle%5D)+AND+%22Water+Res%22%5BJournal%5D)">Methodological approaches for studying the microbial ecology of drinking water distribution systems.</a> Water Res. 65, 134-156 (2014).</li>
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<li>Mojica, K. D., Brussaard, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Factors+affecting+virus+dynamics+and+microbial+host-virus+interactions+in+marine+environments%5BTitle%5D)+AND+%22FEMS+Microbiol+Ecol%22%5BJournal%5D)">Factors affecting virus dynamics and microbial host-virus interactions in marine environments.</a> FEMS Microbiol Ecol. 89 (3), 495-515 (2014).</li>
<li>Graham, E. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbes+as+engines+of+ecosystem+function%3A+when+does+community+structure+enhance+predictions+of+ecosystem+processes%5BTitle%5D)+AND+%22Front+Microbiol%22%5BJournal%5D)">Microbes as engines of ecosystem function: when does community structure enhance predictions of ecosystem processes.</a> Front Microbiol. 7, 214(2016).</li>
<li>Ceballos, R. M., Stacy, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantifying+relative+virulence%3A+when+%C2%B5+max+fails+and+AUC+alone+just+is+not+enough%5BTitle%5D)+AND+%22J+General+Virol%22%5BJournal%5D)">Quantifying relative virulence: when µ max fails and AUC alone just is not enough.</a> J General Virol. 102 (1), 001515(2021).</li>
<li>Hour, S., Pierce, A. J., Heng, S. Y., Plymale, R., Ceballos, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantifying+Resilience+in+Single-Host%2FSingle-Virus+Infections%5BTitle%5D)+AND+%22Appl+Microbiol%22%5BJournal%5D)">Quantifying Resilience in Single-Host/Single-Virus Infections.</a> Appl Microbiol. 5 (1), 18(2025).</li>
<li>Ceballos, R. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+virus+host-ranges+of+the+Fuselloviridae+of+hyperthermophilic+Archaea%3A+implications+for+evolution+in+extreme+environments%5BTitle%5D)+AND+%22Front+Microbiol%22%5BJournal%5D)">Differential virus host-ranges of the Fuselloviridae of hyperthermophilic Archaea: implications for evolution in extreme environments.</a> Front Microbiol. 3, 295(2012).</li>
<li>Ceballos, R. M., Drummond, C. G., Stacy, C. L., Padilla-Crespo, E., Stedman, K. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Host-dependent+differences+in+replication+strategy+of+the+Sulfolobus+spindle-shaped+virus+strain+SSV9+%28aka%2C+SSVK1%29%3A+infection+profiles+in+hosts+of+the+family+Sulfolobaceae%5BTitle%5D)+AND+%22Front+Microbiol%22%5BJournal%5D)">Host-dependent differences in replication strategy of the Sulfolobus spindle-shaped virus strain SSV9 (aka, SSVK1): infection profiles in hosts of the family Sulfolobaceae.</a> Front Microbiol. 11, 1218(2020).</li>
<li>Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., Stanier, R. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Generic+assignments%2C+strain+histories+and+properties+of+pure+cultures+of+cyanobacteria%5BTitle%5D)+AND+%22Microbiology%22%5BJournal%5D)">Generic assignments, strain histories and properties of pure cultures of cyanobacteria.</a> Microbiology. 111 (1), 1-61 (1979).</li>
<li>Kliphuis, A. M., Janssen, M., van den End, E. J., Martens, D. E., Wijffels, R. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Light+respiration+in+Chlorella+sorokiniana%5BTitle%5D)+AND+%22J+Appl+Phycol%22%5BJournal%5D)">Light respiration in Chlorella sorokiniana.</a> J Appl Phycol. 23, 935-947 (2011).</li>
<li>Lea-Smith, D. J., Vasudevan, R., Howe, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Generation+of+marked+and+markerless+mutants+in+model+cyanobacterial+species%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Generation of marked and markerless mutants in model cyanobacterial species.</a> J Vis Exp. (111), e54001(2016).</li>
<li>Noroozi, M., Amozegar, M. A., Rahimi, R., Shahzadeh Fazeli, S. A., Bakhshi Khaniki, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+isolation+and+preliminary+characterization+of+native+cyanobacterial+and+microalgal+strains+from+lagoons+contaminated+with+petroleum+oil+in+Khark+Island%5BTitle%5D)+AND+%22Biol+J+Microorg%22%5BJournal%5D)">The isolation and preliminary characterization of native cyanobacterial and microalgal strains from lagoons contaminated with petroleum oil in Khark Island.</a> Biol J Microorg. 5 (20), 33-41 (2017).</li>
<li>Nübel, U., Garcia-Pichel, F., Muyzer, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((PCR+primers+to+amplify+16S+rRNA+genes+from+cyanobacteria%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">PCR primers to amplify 16S rRNA genes from cyanobacteria.</a> Appl Environ Microbiol. 63 (8), 3327-3332 (1997).</li>
<li>Tragin, M., Lopes dos Santos, A., Christen, R., Vaulot, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Diversity+and+ecology+of+green+microalgae+in+marine+systems%3A+an+overview+based+on+18S+rRNA+gene+sequences%5BTitle%5D)+AND+%22Perspect.+Phycol%22%5BJournal%5D)">Diversity and ecology of green microalgae in marine systems: an overview based on 18S rRNA gene sequences.</a> Perspect. Phycol. 3 (3), 141-154 (2016).</li>
<li>Uchida, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+coupled+assay+system+for+the+lysis+of+cyanobacteria%5BTitle%5D)+AND+%22Japanese+J+Water+Treatment+Biol%22%5BJournal%5D)">A coupled assay system for the lysis of cyanobacteria.</a> Japanese J Water Treatment Biol. 34 (1), 67-75 (1998).</li>
<li>Drummond, C. Characterization of Sulfolobus. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aDrummond%2C+C+%22Spindle-shaped+Virus+replication%22">Spindle-shaped Virus replication</a>. , Portland State University. (2010).</li>
<li>Flores, E., Wolk, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Production%2C+by+filamentous%2C+nitrogen-fixing+cyanobacteria%2C+of+a+bacteriocin+and+of+other+antibiotics+that+kill+related+strains%5BTitle%5D)+AND+%22Arch+Microbiol%22%5BJournal%5D)">Production, by filamentous, nitrogen-fixing cyanobacteria, of a bacteriocin and of other antibiotics that kill related strains.</a> Arch Microbiol. 145, 215-219 (1986).</li>
<li>Wick, C., McCubbin, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterization+of+purified+MS2+bacteriophage+by+the+physical+counting+methodology+used+in+the+integrated+virus+detection+system+%28IVDS%29%5BTitle%5D)+AND+%22Toxicol+Methods%22%5BJournal%5D)">Characterization of purified MS2 bacteriophage by the physical counting methodology used in the integrated virus detection system (IVDS).</a> Toxicol Methods. 9 (4), 245-252 (1999).</li>
<li>Marei, E. M., Elbaz, R., Hammad, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Induction+of+temperate+cyanophages+using+heavy+metal-copper%5BTitle%5D)+AND+%22Int+J+Microbiol+Res+Rev%22%5BJournal%5D)">Induction of temperate cyanophages using heavy metal-copper.</a> Int J Microbiol Res Rev. 5 (5), 472(2013).</li>
<li>Bratchkova, A., Kroumov, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microalgae+as+producers+of+biologically+active+compounds+with+antibacterial%2C+antiviral%2C+antifungal%2C+antialgal%2C+antiprotozoal%2C+antiparasitic+and+anticancer+activity%5BTitle%5D)+AND+%22Acta+Microbiol+Bulgarica%22%5BJournal%5D)">Microalgae as producers of biologically active compounds with antibacterial, antiviral, antifungal, antialgal, antiprotozoal, antiparasitic and anticancer activity.</a> Acta Microbiol Bulgarica. 36 (3), 79-89 (2020).</li>
<li>Storms, Z. J., Teel, M. R., Mercurio, K., Sauvageau, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+virulence+index%3A+a+metric+for+quantitative+analysis+of+phage+virulence%5BTitle%5D)+AND+%22Phage%22%5BJournal%5D)">The virulence index: a metric for quantitative analysis of phage virulence.</a> Phage. 1 (1), 27-36 (2020).</li>
<li>Omata, T., Murata, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+and+characterization+of+three+types+of+membranes+from+the+cyanobacterium+%28blue-green+alga%29+Synechocystis+PCC+6714%5BTitle%5D)+AND+%22Arch+Microbiol%22%5BJournal%5D)">Isolation and characterization of three types of membranes from the cyanobacterium (blue-green alga) Synechocystis PCC 6714.</a> Arch Microbiol. 139, 113-116 (1984).</li>
<li>Wilson, W. H., Joint, I. R., Carr, N. G., Mann, N. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isolation+and+molecular+characterization+of+five+marine+cyanophages+propagated+on+Synechococcus+sp.+strain+WH7803%5BTitle%5D)+AND+%22Appl+Environ+Microbiol%22%5BJournal%5D)">Isolation and molecular characterization of five marine cyanophages propagated on Synechococcus sp. strain WH7803.</a> Appl Environ Microbiol. 59 (11), 3736-3743 (1993).</li>
<li>Grasso, C. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+review+of+cyanophage-host+relationships%3A+Highlighting+cyanophages+as+a+potential+cyanobacteria+control+strategy%5BTitle%5D)+AND+%22Toxins%22%5BJournal%5D)">A review of cyanophage-host relationships: Highlighting cyanophages as a potential cyanobacteria control strategy.</a> Toxins. 14 (6), 385(2022).</li>
<li>Srivastava, A., Gupta, N., Mishra, A. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cyanophages%3A+interacting+mechanism+and+evolutionary+significance%5BTitle%5D)+AND+%22Cyanobacteria%22%5BJournal%5D)">Cyanophages: interacting mechanism and evolutionary significance.</a> Cyanobacteria. , 255-282 (2024).</li>
<li>Cai, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Abundant+and+cosmopolitan+lineage+of+cyanopodoviruses+lacking+a+DNA+polymerase+gene%5BTitle%5D)+AND+%22ISME+J%22%5BJournal%5D)">Abundant and cosmopolitan lineage of cyanopodoviruses lacking a DNA polymerase gene.</a> ISME J. 17 (2), 252-262 (2023).</li>
<li>Komárek, J., Kaštovský, J., Mareš, J., Johansen, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Taxonomic+classification+of+cyanoprokaryotes+%28cyanobacterial+genera%29+2014%2C+using+a+polyphasic+approach%5BTitle%5D)+AND+%22Preslia%22%5BJournal%5D)">Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach.</a> Preslia. 86 (4), 295-335 (2014).</li>
</ol>

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milieubemonstering van fotosynthetische microben en hun virussen: van veld tot laboratorium

Environmental Sampling of Photosynthetic Microbes and Their Viruses: From Field to Lab

Milieubemonstering van fotosynthetische microben en hun virussen: van veld tot laboratorium

Environmental sampling of photosynthetic microorganisms and their viruses plays a critical role in understanding contemporary marine and freshwater ...

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Research

JoVE Journal

Biology

Biologie

De omloopsnelheid van virussen in zee-en zoetwater-systemen kan worden geschat door een reductie en herhaling techniek. De gegevens kunnen de onderzoekers om de tarieven van de virus-gemedieerde microbiële sterfte in aquatische systemen af ​​te leiden.

Het schatten van Virus Productie tarieven in aquatische systemen

Hier presenteren we een uitgebreid protocol om de beschikbaarheid van organische en anorganische voedingsstoffen en de abundantie en structuur van microbiële en virale gemeenschappen in afgelegen mariene omgevingen te beoordelen.

Het ontrafelen van de Unseen Spelers in de Oceaan - A Field Guide to Water chemie en Mariene Microbiologie

Hier presenteren we fenomisch aanpakken voor de functionele karakterisering van putatieve faaggenen. Technieken omvatten een ontwikkelde test die in staat is om het anabole metabolisme van de gastheer te monitoren, de Multi-fenotype Assay Plates (MAPs), naast de gevestigde methode van metabolomics, die in staat is om effecten op katabool metabolisme te meten.

Faag phenomics: Fysiologische Approaches te karakteriseren Novel virale eiwitten

Inzicht in de biologische samenstelling van milieu zwevende deeltjes is belangrijk voor de studie van de significante effecten op de volksgezondheid en verspreiding van de ziekte. Hier, we drie soorten bioaerosol bemonsteringsmethoden en een biologische analyse van airborne microben gebruikt om beter verkennen vanuit de lucht microbiële gemeenschappen onder verschillende omgevingsomstandigheden.

Samenstelling en analyse van de verdeling van Bioaerosols onder verschillende omgevingsomstandigheden

Een citizen science project is ontworpen om inwoners van San Diego te werven om milieumonsters te verzamelen voor SARS-CoV-2. Er is een meertalig webgebaseerd platform gemaakt voor het indienen van gegevens met behulp van een gebruiksvriendelijke interface voor mobiele apparaten. Een laboratoriuminformatiebeheersysteem vergemakkelijkte het verzamelen van duizenden geografisch diverse monsters met realtime resultaattracking.

Swabbing the Urban Environment - Een pijpleiding voor bemonstering en detectie van SARS-CoV-2 uit milieureservoirs

Deze publicatie beschrijft het ontwerp van laboratoriumfotobioreactoren (PBR's) met aanpasbare lichtregimes. De groei van cyanobacteriën of microalgen, met bicarbonaat als koolstofbron, wordt continu bewaakt door de volumetrische zuurstofproductie te meten. Deze PBR's maken snelle, gerepliceerde laboratoriumgroeivergelijkingen mogelijk met weinig tussenkomst van de gebruiker tijdens experimenten.

Werking van laboratoriumfotobioreactoren met online groeimetingen en aanpasbare lichtregimes

Virusconcentratie uit milieuwater- en afvalwatermonsters is een uitdagende taak, voornamelijk uitgevoerd voor de identificatie en kwantificering van virussen. Hoewel er verschillende virusconcentratiemethoden zijn ontwikkeld en getest, tonen we hier de effectiviteit aan van ultrafiltratie en magere melkvlokking voor RNA-virussen met verschillende monstertypen.

Concentratie van virusdeeltjes uit milieuwater- en afvalwatermonsters met behulp van magere melkflocculatie en ultrafiltratie

Betrouwbare en nauwkeurige vormen van veldinzetbare DNA-analyse zijn cruciaal voor veel industrieën. Dit artikel biedt twee methoden voor milieumicrobiële analyse in het veld: 1) de isolatie van microbieel DNA uit omgevingsmonsters met behulp van een biologische extractie-veldkit, en 2) de analyse van DNA via de field qPCR-eenheid.

Microbieel DNA-onderzoek in het veld met behulp van een biologische extractie-veldkit en een veld-qPCR-eenheid

Deze video illustreert de techniek voor de extractie van DNA van de soorten van microben woonachtig zijn in de termiet einddarm. De voorbereiding van een natte glijbaan berg, wat handig is voor het visualiseren van de darm microbiële gemeenschap wordt ook geïllustreerd, en een rondleiding door het soortenrijke darm-omgeving wordt gegeven.

Extraheren van DNA uit de Gut Microben van de Termite (Zootermopsis Angusticollis) en visualiseren Gut microben

Deze video documenten methoden voor het verzamelen van kust-zee watermonsters en de behandeling daarvan voor verschillende downstream-toepassingen, waaronder biomassa-concentratie, nucleïnezuur zuivering, cel overvloed, nutriënten-en trace gas analyses.

Zeewater Bemonstering en Collection

Ontdek methoden voor milieubemonstering van fotosynthetische microben en hun virussen met behulp van elektronenmicroscopie, kwantitatieve PCR en next-generation sequencing. Deze technieken helpen onderzoekers virus-gastheer dynamiek te karakteriseren en voorspellende modellen te ontwikkelen.

Milieubemonstering van fotosynthetische microben en hun virussen: van veld tot laboratorium

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