Name: Bioprinting van hydrogeltumorplakjes als 3D-model voor mantelcellymfoom (Video) | JoVE Journal
Uploaded: 2025-09-12T13:20:00.000+00:00
Duration: 8 min 31 s
Description: Leer bioprinting van hydrogeltumorplakjes voor mantelcellymfoomonderzoek met Jeko-1-cellen. Dit 3D-model bootst de tumor-microomgeving na en verbetert het begrip van medicijnresistentie.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Jain, P., Wang, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mantle+cell+lymphoma+in+2022-a+comprehensive+update+on+molecular+pathogenesis%2C+risk+stratification%2C+clinical+approach%2C+and+current+and+novel+treatments%5BTitle%5D)+AND+%22Am+J+Hematol%22%5BJournal%5D)">Mantle cell lymphoma in 2022-a comprehensive update on molecular pathogenesis, risk stratification, clinical approach, and current and novel treatments.</a> Am J Hematol. 97 (5), 638-656 (2022).</li>
<li>Lee, J., Cuddihy, M. J., Kotov, N. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+cell+culture+matrices%3A+State+of+the+art%5BTitle%5D)+AND+%22Tissue+Eng+Part+B+Rev%22%5BJournal%5D)">Three-dimensional cell culture matrices: State of the art.</a> Tissue Eng Part B Rev. 14 (1), 61-86 (2008).</li>
<li>Wen, T., Wang, J., Shi, Y., Qian, H., Liu, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhibitors+targeting+Bruton%27s+tyrosine+kinase+in+cancers%3A+Drug+development+advances%5BTitle%5D)+AND+%22Leukemia%22%5BJournal%5D)">Inhibitors targeting Bruton's tyrosine kinase in cancers: Drug development advances.</a> Leukemia. 35 (2), 312-332 (2021).</li>
<li>Lee, H. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Phase+1%2F2+study+of+zilovertamab+and+ibrutinib+in+mantle+cell+lymphoma+%28mcl%29+or+chronic+lymphocytic+leukemia+%28cll%29%5BTitle%5D)+AND+%22J+Clin+Oncol%22%5BJournal%5D)">Phase 1/2 study of zilovertamab and ibrutinib in mantle cell lymphoma (mcl) or chronic lymphocytic leukemia (cll).</a> J Clin Oncol. 40 (16_suppl), 7520(2022).</li>
<li>Roué, G., Sola, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Management+of+drug+resistance+in+mantle+cell+lymphoma%5BTitle%5D)+AND+%22Cancers%22%5BJournal%5D)">Management of drug resistance in mantle cell lymphoma.</a> Cancers. 12 (6), 1565(2020).</li>
<li>Araujo-Ayala, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+patient-derived+3d+model+recapitulates+mantle+cell+lymphoma+lymph+node+signaling%2C+immune+profile%2C+and+in+vivo+ibrutinib+responses%5BTitle%5D)+AND+%22Leukemia%22%5BJournal%5D)">A novel patient-derived 3d model recapitulates mantle cell lymphoma lymph node signaling, immune profile, and in vivo ibrutinib responses.</a> Leukemia. 37 (6), 1311-1323 (2023).</li>
<li>Bryant, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+intermediate-grade+%28mantle+cell%29+and+low-grade+%28small+lymphocytic+and+marginal+zone%29+human+non-Hodgkin%27s+lymphomas+xenotransplanted+in+severe+combined+immunodeficiency+mouse+models%5BTitle%5D)+AND+%22Lab+Invest%22%5BJournal%5D)">Development of intermediate-grade (mantle cell) and low-grade (small lymphocytic and marginal zone) human non-Hodgkin's lymphomas xenotransplanted in severe combined immunodeficiency mouse models.</a> Lab Invest. 80 (4), 557-573 (2000).</li>
<li>Andersen, T., Auk-Emblem, P., Dornish, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3d+cell+culture+in+alginate+hydrogels%5BTitle%5D)+AND+%22Microarrays%22%5BJournal%5D)">3d cell culture in alginate hydrogels.</a> Microarrays. 4 (2), 133-161 (2015).</li>
<li>Frantz, C., Stewart, K. M., Weaver, V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+extracellular+matrix+at+a+glance%5BTitle%5D)+AND+%22J+Cell+Sci%22%5BJournal%5D)">The extracellular matrix at a glance.</a> J Cell Sci. 123 (24), 4195-4200 (2010).</li>
<li>Jubelin, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+in+vitro+culture+models+in+oncology+research%5BTitle%5D)+AND+%22Cell+Biosci%22%5BJournal%5D)">Three-dimensional in vitro culture models in oncology research.</a> Cell Biosci. 12 (1), 155(2022).</li>
<li>Hickman, J. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+models+of+cancer+for+pharmacology+and+cancer+cell+biology%3A+Capturing+tumor+complexity+in+vitro%2Fex+vivo%5BTitle%5D)+AND+%22Biotechnol+J%22%5BJournal%5D)">Three-dimensional models of cancer for pharmacology and cancer cell biology: Capturing tumor complexity in vitro/ex vivo.</a> Biotechnol J. 9 (9), 1115-1128 (2014).</li>
<li>Rossi, M., Alviano, F., Righi, S., Sabattini, E., Agostinelli, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+models%3A+A+novel+approach+for+lymphoma+research%5BTitle%5D)+AND+%22J+Cancer+Res+Clin+Oncol%22%5BJournal%5D)">Three-dimensional models: A novel approach for lymphoma research.</a> J Cancer Res Clin Oncol. 148 (4), 753-765 (2022).</li>
<li>Saba, N. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pathogenic+role+of+B-cell+receptor+signaling+and+canonical+nf-%CE%BAb+activation+in+mantle+cell+lymphoma%5BTitle%5D)+AND+%22Blood%22%5BJournal%5D)">Pathogenic role of B-cell receptor signaling and canonical nf-κb activation in mantle cell lymphoma.</a> Blood. 128 (1), 82-92 (2016).</li>
<li>Chiron, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rational+targeted+therapies+to+overcome+microenvironment-dependent+expansion+of+mantle+cell+lymphoma%5BTitle%5D)+AND+%22Blood%22%5BJournal%5D)">Rational targeted therapies to overcome microenvironment-dependent expansion of mantle cell lymphoma.</a> Blood. 128 (24), 2808-2818 (2016).</li>
<li>Nylund, P., Nikkarinen, A., Ek, S., Glimelius, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Empowering+macrophages%3A+The+cancer+fighters+within+the+tumour+microenvironment+in+mantle+cell+lymphoma%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Empowering macrophages: The cancer fighters within the tumour microenvironment in mantle cell lymphoma.</a> Front Immunol. 15, 1373269(2024).</li>
<li>Nygren, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((T-cell+levels+are+prognostic+in+mantle+cell+lymphoma%5BTitle%5D)+AND+%22Clin+Cancer+Res%22%5BJournal%5D)">T-cell levels are prognostic in mantle cell lymphoma.</a> Clin Cancer Res. 20 (23), 6096-6104 (2014).</li>
<li>Medina, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mesenchymal+stromal+cells+protect+mantle+cell+lymphoma+cells+from+spontaneous+and+drug-induced+apoptosis+through+secretion+of+b-cell+activating+factor+and+activation+of+the+canonical+and+non-canonical+nuclear+factor+%CE%BAb+pathways%5BTitle%5D)+AND+%22Haematologica%22%5BJournal%5D)">Mesenchymal stromal cells protect mantle cell lymphoma cells from spontaneous and drug-induced apoptosis through secretion of b-cell activating factor and activation of the canonical and non-canonical nuclear factor κb pathways.</a> Haematologica. 97 (8), 1255-1263 (2012).</li>
<li>Jindal, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Targeting+cers6-as1%2Ffgfr1+axis+as+synthetic+vulnerability+to+constrain+stromal+cells+supported+proliferation+in+mantle+cell+lymphoma%5BTitle%5D)+AND+%22Leukemia%22%5BJournal%5D)">Targeting cers6-as1/fgfr1 axis as synthetic vulnerability to constrain stromal cells supported proliferation in mantle cell lymphoma.</a> Leukemia. 38 (10), 2196-2209 (2024).</li>
<li>Nie, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Establishment+of+a+patient-derived+organoid+culture+platform+for+mantle+cell+lymphoma+drug+sensitivity+and+car+t+cell+anti-tumor+activity+assay%5BTitle%5D)+AND+%22Blood%22%5BJournal%5D)">Establishment of a patient-derived organoid culture platform for mantle cell lymphoma drug sensitivity and car t cell anti-tumor activity assay.</a> Blood. 140 (Supplement 1), 9219-9220 (2022).</li>
<li>Hinton, T. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Three-dimensional+printing+of+complex+biological+structures+by+freeform+reversible+embedding+of+suspended+hydrogels%5BTitle%5D)+AND+%22Sci+Adv%22%5BJournal%5D)">Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels.</a> Sci Adv. 1 (9), e1500758(2015).</li>
<li>Van Der Kuip, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Short-term+culture+of+breast+cancer+tissues+to+study+the+activity+of+the+anticancer+drug+Taxol+in+an+intact+tumor+environment%5BTitle%5D)+AND+%22BMC+Cancer%22%5BJournal%5D)">Short-term culture of breast cancer tissues to study the activity of the anticancer drug Taxol in an intact tumor environment.</a> BMC Cancer. 6 (1), 86(2006).</li>
<li>Scaduto, R. C., Grotyohann, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Measurement+of+mitochondrial+membrane+potential+using+fluorescent+rhodamine+derivatives%5BTitle%5D)+AND+%22Biophys+J%22%5BJournal%5D)">Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives.</a> Biophys J. 76 (1), 469-477 (1999).</li>
<li>Porter, A. G., Jänicke, R. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Emerging+roles+of+caspase-3+in+apoptosis%5BTitle%5D)+AND+%22Cell+Death+Differentiat%22%5BJournal%5D)">Emerging roles of caspase-3 in apoptosis.</a> Cell Death Differentiat. 6 (2), 99-104 (1999).</li>
<li>Huseynov, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Treatment+of+mantle+cell+lymphoma+in+transplant+non-eligible+patients%5BTitle%5D)+AND+%22Hematol+Transfus+Cell+Ther%22%5BJournal%5D)">Treatment of mantle cell lymphoma in transplant non-eligible patients.</a> Hematol Transfus Cell Ther. 44, S2-S3 (2022).</li>
<li>Augustine, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3d+bioprinted+cancer+models%3A+Revolutionizing+personalized+cancer+therapy%5BTitle%5D)+AND+%22Transl+Oncol%22%5BJournal%5D)">3d bioprinted cancer models: Revolutionizing personalized cancer therapy.</a> Transl Oncol. 14 (4), 101015(2021).</li>
<li>Budharaju, H., Sundaramurthi, D., Sethuraman, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Embedded+3d+bioprinting+%E2%80%93+an+emerging+strategy+to+fabricate+biomimetic+%26+large+vascularized+tissue+constructs%5BTitle%5D)+AND+%22Bioact+Mater%22%5BJournal%5D)">Embedded 3d bioprinting – an emerging strategy to fabricate biomimetic & large vascularized tissue constructs.</a> Bioact Mater. 32, 356-384 (2024).</li>
<li>Seaman, K., Sun, Y., You, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Recent+advances+in+cancer-on-a-chip+tissue+models+to+dissect+the+tumour+microenvironment%5BTitle%5D)+AND+%22Med-X%22%5BJournal%5D)">Recent advances in cancer-on-a-chip tissue models to dissect the tumour microenvironment.</a> Med-X. 1 (1), 11(2023).</li>
<li>Davies, E. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Capturing+complex+tumour+biology+in+vitro%3A+Histological+and+molecular+characterisation+of+precision+cut+slices%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Capturing complex tumour biology in vitro: Histological and molecular characterisation of precision cut slices.</a> Sci Rep. 5 (1), 17187(2015).</li>
<li><a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Millipore+PICM03050%3A+Millicell+Standing+Cell+Culture+Inserts+Data+Sheet%22">Millipore PICM03050: Millicell Standing Cell Culture Inserts Data Sheet</a>. , Merck KGaA. Darmstadt, Germany. (2025).</li>
<li>Lamaison, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+novel+3d+culture+model+recapitulates+primary+fl+b-cell+features+and+promotes+their+survival%5BTitle%5D)+AND+%22Blood+Adv%22%5BJournal%5D)">A novel 3d culture model recapitulates primary fl b-cell features and promotes their survival.</a> Blood Adv. 5 (23), 5372-5386 (2021).</li>
<li>Kramer, R. H., Rosen, S. D., McDonald, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Basement-membrane+components+associated+with+the+extracellular+matrix+of+the+lymph+node%5BTitle%5D)+AND+%22Cell+Tissue+Res%22%5BJournal%5D)">Basement-membrane components associated with the extracellular matrix of the lymph node.</a> Cell Tissue Res. 252 (2), 367-375 (1988).</li>
<li>Kaldjian, E. P., Gretz, J. E., Anderson, A. O., Shi, Y., Shaw, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Spatial+and+molecular+organization+of+lymph+node+T+cell+cortex%3A+A+labyrinthine+cavity+bounded+by+an+epithelium-like+monolayer+of+fibroblastic+reticular+cells+anchored+to+basement+membrane-like+extracellular+matrix%5BTitle%5D)+AND+%22Int+Immunol%22%5BJournal%5D)">Spatial and molecular organization of lymph node T cell cortex: A labyrinthine cavity bounded by an epithelium-like monolayer of fibroblastic reticular cells anchored to basement membrane-like extracellular matrix.</a> Int Immunol. 13 (10), 1243-1253 (2001).</li>
<li>German, S. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Plug-and-play+lymph+node-on-chip%3A+Secondary+tumor+modeling+by+the+combination+of+cell+spheroid%2C+collagen+sponge%2C+and+T-cells%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Plug-and-play lymph node-on-chip: Secondary tumor modeling by the combination of cell spheroid, collagen sponge, and T-cells.</a> Int J Mol Sci. 24 (4), 3183(2023).</li>
<li>Benton, G., Arnaoutova, I., George, J., Kleinman, H. K., Koblinski, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Matrigel%3A+From+discovery+and+ecm+mimicry+to+assays+and+models+for+cancer+research%5BTitle%5D)+AND+%22Adv+Drug+Deliver+Rev%22%5BJournal%5D)">Matrigel: From discovery and ecm mimicry to assays and models for cancer research.</a> Adv Drug Deliver Rev. 79-80, 3-18 (2014).</li>
<li>Ogassavara, B., Tucunduva Neto, R. R., De Souza, R. R., Tucunduva, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ultrasound+evaluation+of+the+morphometric+patterns+of+lymph+nodes+of+the+head+and+neck+in+young+and+middle-aged+individuals%5BTitle%5D)+AND+%22Radiol+Brasileira%22%5BJournal%5D)">Ultrasound evaluation of the morphometric patterns of lymph nodes of the head and neck in young and middle-aged individuals.</a> Radiol Brasileira. 49 (4), 225-228 (2016).</li>
<li>Huang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Extracellular+matrix+and+its+therapeutic+potential+for+cancer+treatment%5BTitle%5D)+AND+%22Signal+Transduct+Target+Ther%22%5BJournal%5D)">Extracellular matrix and its therapeutic potential for cancer treatment.</a> Signal Transduct Target Ther. 6 (1), 1-24 (2021).</li>
<li>Subrahmanyam, N., Ghandehari, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Harnessing+extracellular+matrix+biology+for+tumor+drug+delivery%5BTitle%5D)+AND+%22J+Personalized+Med%22%5BJournal%5D)">Harnessing extracellular matrix biology for tumor drug delivery.</a> J Personalized Med. 11 (2), 88(2021).</li>
<li>Ahmad, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Prospective+of+extracellular+matrix+and+drug+correlations+in+disease+management%5BTitle%5D)+AND+%22Asian+J+Pharma+Sci%22%5BJournal%5D)">Prospective of extracellular matrix and drug correlations in disease management.</a> Asian J Pharma Sci. 16 (2), 147-160 (2021).</li>
</ol>

```html
<table><tbody><tr><td>&micro;-slide 8 Well Glass Bottom</td><td>ibidi GmbH</td><td>80827</td><td>Glass bottom slides used for live imaging of hydrogel slices</td></tr><tr><td>10x PBS</td><td>Carl Roth GmbH + Co.KG</td><td>9150.1</td><td>Bioink component</td></tr><tr><td>10X RPMI-1640 Medium</td><td>Sigma-Aldrich</td><td>R1145</td><td>Bioink component</td></tr><tr><td>2.5 ml Gastight Syringe Model 1002 TLL</td><td>Hamilton</td><td>81420</td><td>Syringe for bioprinting</td></tr><tr><td>3D Extrusion printer, PrintrBot Simple</td><td>Printrbot</td><td></td><td>Self-modified to allow for printing of bioinks</td></tr><tr><td>6-well plate</td><td>Th. Geyer GmbH &amp; Co.KG</td><td>7696790</td><td>Culture of the hydrogel slices</td></tr><tr><td>Bottle top vacuum filtration systems, PES membrane, 500 ml, 0.22 &micro;m pore size, 70&nbsp;mm diameter membrane</td><td>Corning Inc.</td><td>513-3355</td><td>Sterile filtration of buffers</td></tr><tr><td>Calcium chloride dehydrate</td><td>Carl Roth GmbH + Co.KG</td><td>T885.2</td><td>Component of wash buffer and gelatin slurry</td></tr><tr><td>Collagen I from rat</td><td>Corning Inc.</td><td>CLS354249</td><td>Bioink component</td></tr><tr><td>Cura Version 15.04.5 software for 3D printing</td><td>Ultimaker</td><td></td><td>Software used for 3D printing</td></tr><tr><td>DRAQ5</td><td>Biostatus Ltd.</td><td>DR50050</td><td>Stain for live imaging of hydrogel slices</td></tr><tr><td>Ethylenediaminetetraacetic acid (EDTA) disodium salt dihydrate</td><td>Sigma-Aldrich</td><td>E5134</td><td>Component of alginate dissolving buffer</td></tr><tr><td>EVE Automatic Cell Counter</td><td>Nano EnTek Inc.</td><td>10027-452</td><td>Cell Counter</td></tr><tr><td>Falcon Bacteriological Petri Dishes with Lid, 35 mm diameter</td><td>Falcon</td><td>351008</td><td>Filled with gelatin slurry to form the support bath for bioprinting</td></tr><tr><td>Falcon Tubes, 50 mL</td><td>Sarstedt AG &amp; Co.KG</td><td>62.547.254</td><td></td></tr><tr><td>Fetal calf serum</td><td>Sigma-Aldrich</td><td>S0615</td><td>Cell culture medium supplement</td></tr><tr><td>Gelatin from porcine skin</td><td>Sigma-Aldrich</td><td>G1890</td><td>Main component of the gelatin slurry</td></tr><tr><td>HEPES, Free Acid, Molecular Biology Grade</td><td>Merck KGaA</td><td>CAS 7365-45-9</td><td>Component of wash buffer and gelatin slurry</td></tr><tr><td>Jeko-1 MCL cell line</td><td>ATCC</td><td>CRL-3006</td><td>MCL cell line</td></tr><tr><td>KIMTECH Science Precision wipes</td><td>Kimberly Clark Corporation</td><td>7551</td><td>Used to draw excess water from the gelatin slurry and to remove droplets from the 3D-printer nozzle</td></tr><tr><td>LAS X software Version 3.5.7.23225</td><td>Leica</td><td></td><td>Software used for 3D reconstructions</td></tr><tr><td>Leica TCS SP8 Confocal laser scanning microscope</td><td>Leica</td><td>TCS SP8</td><td>Confocal microscope for live imaging</td></tr><tr><td>Mason Jar Regular Pint 16 oz</td><td>Ball Corporation</td><td>G001</td><td>Jar in which the gelatin slurry is prepared</td></tr><tr><td>Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix</td><td>Corning Inc.</td><td>356231</td><td>Bioink component</td></tr><tr><td>Millicell cell culture insert, 0.4 &micro;m pore size</td><td>Merck KGaA</td><td>PICMORG 50</td><td>Filter support for hydrogel slices</td></tr><tr><td>NucView 405 Caspase-3 Substrate, 1 mM in 1x PBS</td><td>Biotium</td><td>10405</td><td>Stain for live imaging of hydrogel slices</td></tr><tr><td>Oster Professional Series Blender</td><td>Oster</td><td>BPST02-B00</td><td>Blender to generate gelatin slurry</td></tr><tr><td>Penicillin/Streptamycin</td><td>Gibco</td><td>15140163</td><td>Supplement for MCL cell culture medium</td></tr><tr><td>Quant-iT PicoGreen dsDNA Assay-Kits und dsDNA-Reagenzien</td><td>Invitrogen</td><td>P7589</td><td>Stain for live imaging of hydrogel slices</td></tr><tr><td>Rotina 35 R</td><td>Hettich</td><td>24573</td><td>Centrifugation of cells</td></tr><tr><td>ROTINA 38/38R centrifuge</td><td>Hettich</td><td>Z720109</td><td>Centrifugtion of gelatin slurry</td></tr><tr><td>RPMI-1640+GlutaMAX</td><td>Gibco</td><td>61870036</td><td>Base of culture medium for MCL cells</td></tr><tr><td>Sodium alginate</td><td>FMC Cooperation</td><td>GQ9109901</td><td>Bioink component</td></tr><tr><td>Sodium chloride</td><td>Merck KGaA</td><td>1.06404</td><td>Component of alginate dissolving buffer</td></tr><tr><td>Sodium citrate</td><td>Carl Roth GmbH + Co.KG</td><td>3580.1</td><td>Component of alginate dissolving buffer</td></tr><tr><td>Sterican blunt application cannulas 21G 0.8x22 mm</td><td>B.Braun</td><td>9180109</td><td>Nozzle for 3D-printing</td></tr><tr><td>Tetramethylrhodamin-methylester-perchlorat (TMRM)</td><td>Sigma-Aldrich</td><td>T5428</td><td>Stain for live imaging of hydrogel slices</td></tr><tr><td>Trypan blue, 0.4%</td><td>Sigma-Aldrich</td><td>T8154</td><td>Stain for viability analysis</td></tr><tr><td>Waterproof thermometer</td><td>VWR</td><td>620-2099</td><td>Thermometer to monitor the temperature of the gelatin slurry</td></tr></tbody></table>
```

bioprinting van hydrogeltumorplakjes als 3d-model voor mantelcellymfoom

Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma

Bioprinting van hydrogeltumorplakjes als 3D-model voor mantelcellymfoom

Mantle cell lymphoma (MCL) is a rare, aggressive B-cell neoplasm that frequently relapses and only shows a limited response to conventional chemotherapy. ...

bioprinting-hydrogel-tumor-slices-as-3d-model-for-mantle-cell

Research

JoVE Journal

Cancer Research

Kankeronderzoek

Van patiënten afgeleide xenografts van glioblastoma multiforme kunnen worden geminiaturiseerd tot levende microtumoren met behulp van een 3D-menselijke biogel-kweeksysteem. Deze in vivo-achtige 3D-tumortest is geschikt voor het testen van de reactie op geneesmiddelen en moleculair profileren, inclusief kinoomanalyse.

Genereren van Microtumors Met behulp van 3D Human Biogel Cultuur System en Patient afgeleide glioblastoma Cellen voor Kinomic Profiling and Drug Response Testen

Mantel cel lymfoom (MCL) is een moeilijk te behandelen stoornis van de B-cel en het is even moeilijk vast te stellen een muismodel van xenograft van primaire MCL te bestuderen en ontwikkelen van therapeutics. Hier beschrijven we de succesvolle invoering van MCL xenografts bij muizen om te helpen begrijpen van de onderliggende biologie.

Engraftment van de tumor in een muismodel van Xenograft van menselijke mantel cel lymfoom

Hier, wordt een methode beschreven voor de bereiding van 3-dimensionale (3D) sferoïde co cultuur van pancreatic kankercellen en fibroblasten, gevolgd door meting van metabolische functies met behulp van een extracellulaire flux-analyzer.

Voorbereiding en metabole kwantitatieve analyse van 3-dimensionale sferoïde co culturen van Pancreatic kankercellen en fibroblasten

Wij ontwikkeld een heterogene borst kanker model bestaande uit vereeuwigd tumor en fibroblast cellen ingebed in een bioprintable alginaat/gelatine bioink. Het model recapituleert de tumor in vivo communicatie en vergemakkelijkt de vorming van meercellige tumor spheroïden, inzicht in de mechanismen van tumorvorming rijden oplevert.

Bioprintable alginaat/gelatine Hydrogel 3D In Vitro modelsystemen induceren sferoïde celvorming

Hier gepresenteerd is een methode voor de 3D bioprinten van gelatine methacryloyl.

Protocollen van 3D Bioprinting van gelatine Methacryloyl hydrogel gebaseerde Bioinks

Dit protocol toont methoden om een uitgebreide in vitro cultuur van patiënt-afgeleide xenografts (PDX) mogelijk te maken. Een stap verbetert de algehele levensvatbaarheid van meercellige clusterculturen in 3D hydrogels, door eenvoudige verwijdering van niet-levensvatbare enkele cellen. Een secundaire stap toont best practices voor PDX-cultuur in een geperfundeerd microfluïdisch platform.

Verbeterde levensvatbaarheid voor Ex vivo 3D Hydrogel Culturen van patiënt-afgeleide Xenografts in een perfused microfluïdisch platform

Dit protocol presenteert een 3D biomimetisch model met bijbehorend fibrotisch stromaal compartiment. Bereid met fysiologisch relevante hydrogels in verhoudingen die de biofysische eigenschappen van de stromale extracellulaire matrix nabootsen, een actieve bemiddelaar van cellulaire interacties, tumorgroei en uitzaaiingen.

Een biomimetisch model voor leverkanker om tumor-stroma-interacties te bestuderen in een 3D-omgeving met afstembare bio-fysische eigenschappen

Het doel van dit protocol is om borsteptheelcellen rechtstreeks bioprinten als meercellige sferoïden op voorgevormde endotheelnetwerken om snel 3D-borst-endotheel co-cultuurmodellen te creëren die kunnen worden gebruikt voor geneesmiddelenscreeningsstudies.

Directe bioprinting van 3D-meercellige borstsferoïden op endotheelnetwerken

Hypoxie is een kenmerk van tumormicromilieu en speelt een cruciale rol bij de progressie van kanker. Dit artikel beschrijft het fabricageproces van een hypoxische kanker-op-een-chip op basis van 3D-celprinttechnologie om een hypoxiegerelateerde pathologie van kanker samen te vatten.

3D-celgeprinte hypoxische kanker-op-een-chip voor het samenvatten van pathologische progressie van solide kanker

Het huidige protocol beschrijft een experimenteel platform om de effecten van mechanische en biochemische signalen op chemotherapeutische reacties van patiënt-afgeleide glioblastoomcellen in 3D matrix-mimetische culturen te beoordelen met behulp van een op maat gemaakt UV-verlichtingsapparaat dat high-throughput photocrosslinking van hydrogels met instelbare mechanische kenmerken mogelijk maakt.

Hydrogel-arrays maken een verhoogde doorvoer mogelijk voor screeningseffecten van matrixcomponenten en therapeutica in 3D-tumormodellen

We presenteren een methode voor het repliceren van de micro-omgeving van de glioomtumor aan het invasieve front die interstitiële vloeistofstroom omvat. Dit model is een hyaluronzuur-collageen I hydrogel in een weefselkweekinzetstuk waar een vloeistofdrukkop op kan worden aangebracht. Invasie kan worden gekwantificeerd en cellen kunnen worden geïsoleerd of gelyseerd.

Een fotopolymeriseerbaar hyaluronzuur-collageenmodel van de invasieve glioommicro-omgeving met interstitiële stroom

Leer bioprinting van hydrogeltumorplakjes voor mantelcellymfoomonderzoek met Jeko-1-cellen. Dit 3D-model bootst de tumor-microomgeving na en verbetert het begrip van medicijnresistentie.

Bioprinting van hydrogeltumorplakjes als 3D-model voor mantelcellymfoom

Meer video's verkennen