Name: Snelle dissectie en dissociatie van het reukepitheel van de muis voor suspensies met één kern (Video) | JoVE Journal
Uploaded: 2025-08-01T14:00:00.000+00:00
Duration: 3 min 42 s
Description: Leer snelle dissectie en dissociatie van het reukepitheel van de muis voor suspensies met één kern. Deze methode ondersteunt single-nucleus RNA-sequencing en versnelt genetisch onderzoek naar het reukzintuig.

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snelle dissectie en dissociatie van het reukepitheel van de muis voor suspensies met één kern

Rapid Dissection and Dissociation of the Mouse Olfactory Epithelium for Single-Nucleus Suspensions

Snelle dissectie en dissociatie van het reukepitheel van de muis voor suspensies met één kern

The murine olfactory epithelium is the initial entry point of the olfactory system, housing various cell types that include olfactory sensory neurons, ...

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Fotolyse van gekooide verbindingen maakt de productie van snelle en gelokaliseerde toename van de concentratie van diverse fysiologisch actieve verbindingen. Hier laten we zien hoe de patch-clamp opnames gecombineerd met fotolyse van gekooide cAMP of gekooid Ca voor de studie van olfactorische transductie in gedissocieerde muis olfactorische sensorische neuronen te verkrijgen.

Flash fotolyse van Gekooide verbindingen in de Cilia van olfactorische sensorische neuronen

The video demonstrates the isolation of oculomotor, trochlear, and spinal motor neurons from a transgenic mouse embryo. The embryo expresses motor neurons labeled with a green fluorescent protein (GFP) for easy visualization and identification. The motor neurons are surgically isolated under a fluorescence microscope and maintained in a culture medium.

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from a Mouse Embryo

This video demonstrates a method to isolate primary oligodendrocyte cells from a neural cell suspension obtained from a mouse pup using magnetic separation. The technique employs magnetic beads coated with antibodies specific to oligodendrocyte antigens, enabling selective isolation of these cells.

Isolating Murine Primary Oligodendrocytes Using Immunomagnetic Beads

This video demonstrates electrophysiological recording of olfactory sensory neurons (OSNs) in mice using a perforated patch-clamp technique. The olfactory epithelium, containing OSNs with fluorophore-tagged odor receptors, is isolated from a mouse and secured in a recording chamber. A recording pipette is clamped to the dendritic knob of an OSN, and nystatin within the pipette perforates the membrane to form electrical connectivity with the cell. Finally, another pipette is used to deliver an...

Perforated Patch-Clamp Recording of Olfactory Sensory Neurons in Mice

Cilia van olfactorische sensorische neuronen bevatten eiwitten van de signaaltransductiecascade, maar een gedetailleerde ruimtelijke analyse van hun verdeling is moeilijk in cryosecties. We beschrijven hier een geoptimaliseerde aanpak voor hele montage-etikettering en en face visualisatie van ciliaire eiwitten.

Hele Mount Labeling van Cilia in het Main Olfactorische Systeem van Muizen

Het analyseren van de fysiologische eigenschappen van olfactorische sensorische neuronen kent nog steeds technische beperkingen. Hier registreren we ze door middel van perforated patch-clamp in een intacte voorbereiding van het olfactorische epitheel in gene-targeted muizen. Deze techniek maakt de karakterisering mogelijk van membraaneigenschappen en reacties op specifieke liganden van neuronen die gedefinieerde olfactorische receptoren tot expressie brengen.

Geperforeerde Patch-clamp-opname van de muis olfactorische sensorische neuronen in Intact neuroepithelium: functionele analyse van neuronen uiten van een geïdentificeerd Odorant Receptor

Oligodendrocyten zijn de myeliniserende cellen van het centrale zenuwstelsel. Dit protocol beschrijft een methode voor het isoleren en kweken van hun precursoren, oligodendrocyten-voorlopercellen, uit rattencortex, evenals een snelle en betrouwbare kwantitatieve methode om oligodendrogenese in vitro te evalueren als reactie op experimentele factoren.

Voorbereiding van de Rat Oligodendrocyte Progenitor culturen en kwantificering van Oligodendrogenesis gebruik van Dual-infrarood fluorescentie Scanning

We beschrijven de immunomagnetic isolatie van primaire muisknop oligodendrocyten, waarmee het snelle en specifieke isolement van de cellen voor in vitro cultuur.

Snelle en specifieke Immunomagnetic isolatie van de primaire oligodendrocyten muis

Een methode voor microdissectie van de eileider van de muis die het mogelijk maakt om de afzonderlijke segmenten te verzamelen met behoud van RNA-integriteit wordt gepresenteerd. Bovendien wordt een niet-enzymatische ovductale celdissociatieprocedure beschreven. De methoden zijn geschikt voor latere gen- en eiwitanalyse van de functioneel verschillende eileidersegmenten en gedissocieerde eileidercellen.

Microdissectie en dissociatie van de murine-eileider: individuele segmentidentificatie en eencellige isolatie

Het succes van single-cell/single-nuclei transcriptomics en multi-omics hangt grotendeels af van de kwaliteit van cellen/kernen. Daarom moet het isoleren van cellen/kernen uit weefsel en hun zuivering sterk gestandaardiseerd zijn. Dit protocol beschrijft de bereiding van kernen uit de hersenen en het beenmerg voor stroomafwaartse single-nuclei multioomanalyse.

Hoogwaardige voorbereiding van hersen- en beenmergkernen voor multioomtesten met één kern

Leer snelle dissectie en dissociatie van het reukepitheel van de muis voor suspensies met één kern. Deze methode ondersteunt single-nucleus RNA-sequencing en versnelt genetisch onderzoek naar het reukzintuig.

Snelle dissectie en dissociatie van het reukepitheel van de muis voor suspensies met één kern

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