Name: Beeldgeleide in vivo tracking van getransplanteerde distale longepitheliale voorlopercellen voor longfibrose met behulp van magnetische beeldvorming (Video) | JoVE Journal
Uploaded: 2025-06-27T14:40:00.000+00:00
Duration: 7 min 14 s
Description: Leer beeldgeleide in vivo tracking van distale longepitheliale voorlopercellen voor longfibrose met magnetische beeldvorming. Deze methode optimaliseert celtherapie door niet-invasieve monitoring van celretentie en -distributie.

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<table><tbody><tr><td>1.7 mL microcentrifuge tube</td><td>DOT Scientific</td><td>RN1700-GMT</td><td>Voor celcollectie en het bereiden van BLM-verdunningen</td></tr><tr><td>Anti-Dextran antilichaam</td><td>STEMCELL Technologies</td><td>60026</td><td>Primair antilichaam</td></tr><tr><td>BD Luer-Lok Spuit</td><td>Becton, Dickinson</td><td>309628</td><td>Voor luchtinjecties tijdens intratracheale instillatie</td></tr><tr><td>Cytospin 3</td><td>Thermo Shandon</td><td>74010121GB</td><td></td></tr><tr><td>DNase I</td><td>Millipore-Sigma</td><td>10104159001</td><td></td></tr><tr><td>Dulbecco's fosfaatgebufferde zoutoplossing (DPBS -/-) </td><td>Gibco</td><td>14190250</td><td></td></tr><tr><td>Fetaal runder serum </td><td>ThermoFisher Scientific</td><td>A5670701</td><td></td></tr><tr><td>Gentamicine </td><td>Gibco</td><td>15750060</td><td></td></tr><tr><td>Geit anti-Muis IgG (H+L) Cross-Adsorbed Secundair Antilichaam, Alexa Fluor™ 568</td><td>ThermoFisher Scientific</td><td>A-11004</td><td>Secundair antilichaam</td></tr><tr><td>Geit serum</td><td>Millipore-Sigma</td><td>G9023</td><td></td></tr><tr><td>Isofluraan</td><td>Covetrus</td><td>11695067772</td><td>Gasanesthesie</td></tr><tr><td>Isofluraanverdamper</td><td>SOMNI Scientific</td><td>VS6002</td><td>Anesthesie apparaat</td></tr><tr><td>Momentum</td><td>Magnetic Insight Inc</td><td></td><td>Preklinische Magnetische Deeltjes Beeldvorming (MPI) scanner</td></tr><tr><td>Muis intubatiekit</td><td>OHAN</td><td>Ohan-201</td><td>Complete intubatiekit met alle benodigde accessoires</td></tr><tr><td>NOD/SCID Muizen</td><td>Jackson Laboratory</td><td>RRID:IMSR_JAX:001303</td><td>Immunodeficiënte muismodel</td></tr><tr><td>Penicilline/Streptomycine </td><td>Gibco</td><td>15140122</td><td></td></tr><tr><td>PneumaCult-Ex Plus Medium </td><td>STEMCELL Technologies</td><td>5040</td><td></td></tr><tr><td>ProLong Diamond Antifade Mountant met DAPI </td><td>Invitrogen</td><td>P36962</td><td>Montage medium voor immunogeverfde slides</td></tr><tr><td>Protease uit Streptomyces griseus </td><td>Millipore-Sigma</td><td>P5147</td><td></td></tr><tr><td>Chirurgische kit</td><td></td><td></td><td>Blunt-end pincetten gebruikt voor het vasthouden van de tong van de muis</td></tr><tr><td>Tris-Buffered Saline met Tween20 (TBST)</td><td>ThermoFisher Scientific</td><td>28360</td><td></td></tr><tr><td>TrypLE Express Enzym </td><td>ThermoFisher Scientific</td><td>12604021</td><td></td></tr><tr><td>VivoTrax Plus</td><td>Magnetic Insight</td><td>MIVTP01</td><td>IJzeroxide tracer voor cellabeling in vitro en daaropvolgende MPI</td></tr></tbody></table>

beeldgeleide in vivo tracking van getransplanteerde distale longepitheliale voorlopercellen voor longfibrose met behulp van magnetische beeldvorming

Image-guided In Vivo Tracking of Transplanted Distal Lung Epithelial Progenitor Cells for Pulmonary Fibrosis Using Magnetic Particle Imaging

Beeldgeleide in vivo tracking van getransplanteerde distale longepitheliale voorlopercellen voor longfibrose met behulp van magnetische beeldvorming

Pulmonary fibrosis (PF) is a progressive and chronic lung disease characterized by repeated alveolar epithelial injury that leads to excessive ...

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Het volgen van cellen met behulp van MRI heeft opgedaan opmerkelijke aandacht in de afgelopen jaren. Dit protocol beschrijft de etikettering van dendritische cellen met fluor...

Monitoring Dendritische cel-migratie met behulp van<sup&gt; 19</sup&gt; F /<sup&gt; 1</sup&gt; H Magnetic Resonance Imaging

Combinatorische 5 fluorescerende eiwitten markering van hematopoietische stamcellen en voorlopercellen maakt in vivo klonale volgen via confocale en twee-foton microscopie, het verstrekken van inzicht in het beenmerg hematopoietische architectuur tijdens de regeneratie. Deze methode maakt het mogelijk niet-invasieve lot in kaart brengen van spectraal-gecodeerde HSPCs-afgeleide cellen in intacte weefsels voor langere perioden na de transplantatie.

In vivo Klonale Tracking van hematopoietische stamcellen en progenitorcellen Gekenmerkt door Five fluorescerende eiwitten met behulp van confocale microscopie en Multiphoton

Beschrijven we een niet-invasieve multimodale aanpak op basis van Micro-CT en fluorescentie moleculaire tomografie voor longitudinale beoordelingvan de muismodel van lung fibrosis geïnduceerd door dubbele intratracheale instillatietest van bleomycine imaging.

Een multimodale Imaging aanpak op basis van Micro-CT en fluorescentie moleculaire tomografie voor longitudinale beoordeling van bleomycine-geïnduceerde Lung Fibrosis in muizen

Dit artikel beschrijft de generatie van een orthotopic muismodel van menselijke pleura mesothelioom door implantatie van H2052/484 mesothelioom cellen in de pleuraholte van immuungecompromitteerde athymische muizen. De longitudinale monitoring van de ontwikkeling van intrapleurale tumoren werd beoordeeld door niet-invasieve multimodale [18F] -2-Fluoro-2-deoxy-D-glucose positron emissie tomografie en computertomografie beeldvorming.

Implantatie en monitoring door PET/CT van een Orthothematisch model van menselijk pleura mesothelioom in Athymic muizen

Gepresenteerd hier is een protocol voor niet-invasieve mesenchymale stamcel (MSC) levering en tracking in een muismodel van traumatisch hersenletsel. Superparamagnetische ijzeroxide nanodeeltjes worden gebruikt als een magnetische resonantie imaging (MRI) sonde voor MSC labeling en niet-invasieve in vivo tracking na intranasale levering met behulp van real-time MRI.

Het volgen van superparamagnetische ijzer oxide-gelabelde mesenchymale stamcellen met behulp van MRI na intranasale levering in een traumatisch hersenletsel Murine model

Hier presenteren we een protocol om de getransplanteerde linkerlong van de muis intravitaal in beeld te brengen met behulp van twee-fotonmicroscopie. Dit is een waardevol hulpmiddel voor het bestuderen van cellulaire dynamiek en interacties in real-time na longtransplantatie bij muizen.

Intravitale twee-fotonmicroscopie van de getransplanteerde muizenlong

Deze studie presenteert een orthotopisch model voor niet-kleincellige longkanker (NSCLC) op basis van intrapulmonale inoculatie van meercellige sferoïden van fluorescerende A549-iRFP-cellen. Het model recapituleert klinische NSCLC-stadia en reageert op cisplatine, volgens dynamische in vivo monitoring van fluorescentie met lange golflengten.

Constructie van een orthotopisch xenotransplantaatmodel van niet-kleincellige longkanker dat ziekteprogressie nabootst en geneesmiddelactiviteiten voorspelt

Leer beeldgeleide in vivo tracking van distale longepitheliale voorlopercellen voor longfibrose met magnetische beeldvorming. Deze methode optimaliseert celtherapie door niet-invasieve monitoring van celretentie en -distributie.

Beeldgeleide in vivo tracking van getransplanteerde distale longepitheliale voorlopercellen voor longfibrose met behulp van magnetische beeldvorming (Video) | JoVE Journal

Beeldgeleide in vivo tracking van getransplanteerde distale longepitheliale voorlopercellen voor longfibrose met behulp van magnetische beeldvorming

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