Name: Een muismodel om de structurele en functionele resultaten op lange termijn te evalueren na de omkering van langdurige unilaterale ureterobstructie (Video) | JoVE Journal
Uploaded: 2025-07-18T14:30:00.000+00:00
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Description: Leer een gestandaardiseerd muismodel voor omkeerbare unilaterale ureterobstructie om langetermijnfunctionele en structurele nierherstel te evalueren. Deze methode vereenvoudigt chirurgische procedures voor bredere toegankelijkheid in onderzoekslaboratoria.

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</ol>

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een muismodel om de structurele en functionele resultaten op lange termijn te evalueren na de omkering van langdurige unilaterale ureterobstructie

A Mouse Model to Evaluate the Long-Term Structural and Functional Outcomes after the Reversal of Prolonged Unilateral Ureteric Obstruction

Een muismodel om de structurele en functionele resultaten op lange termijn te evalueren na de omkering van langdurige unilaterale ureterobstructie

Clinical management of patients with urinary tract obstruction (UTO) requires early intervention to reverse the cause of the obstruction, but despite ...

a-mouse-model-to-evaluate-long-term-structural-functional-outcomes

Research

JoVE Journal

Medicine

Geneeskunde

This video demonstrates the procedure to generate a unilateral ureteral obstruction mouse model. The procedure involves cutting one of the ureters of the mouse to obstruct urine flow.

Unilateral Ureteral Obstruction Murine Model: A Surgical Procedure to Obstruct the Urinary Flow from a Kidney in Mouse Models

Het muismodel van onomkeerbare eenzijdige ureterische obstructie (UUO) wordt gepresenteerd samen met het model van omkeerbare UUO waarbij de ureterische obstructie wordt verlicht door anastomose van de afgesneden ureter in de blaas. Deze modellen maken de studie van nierontsteking en littekenvorming mogelijk, evenals weefselremodellering.

Een muizenmodel van reversibele en irreversibele Eenzijdige Ureteric Obstructie

We beschrijven een methode voor het evalueren van de microRNA-expressie in de nieren van muizen met eenzijdige ureterale obstructie (UUO) door kwantitatieve reverse-transcriptie polymerase kettingreactie. Dit protocol is geschikt voor het bestuderen van niermicroRNA expressieprofielen bij muizen met UUO en in de context van andere pathologische aandoeningen.

Kwantitatieve real-time PCR-evaluatie van microRNA-expressies in muisnier met eenzijdige ureterale obstructie

Hier presenteren we een protocol met behulp van 2-fotonmicroscopie in München Wistar Fromter-ratten met oppervlakteglomeruli om de effecten van langdurige ureterale obstructie op glomerulaire dynamiek en functie te kwantificeren.

2-fotonenmicroscopie gebruiken om de effecten van chronische unilaterale ureterale obstructie op glomerulaire processen te kwantificeren

Het huidige protocol beschrijft een stapsgewijs, reproduceerbaar model van eenzijdige ureterobstructie.

Een onrijp muizenmodel van omkeerbare unilaterale ureterobstructie

Dit protocol beschrijft een methode voor het induceren van unilaterale ureterobstructie (UUO) bij muizen om de progressie van weefselfibrose bij obstructieve nefropathie te bestuderen. Het omvat chirurgische ingrepen, postoperatieve zorg en methoden voor de beoordeling van fibrose.

Unilateraal ureterobstructiemodel voor het onderzoeken van interstitiële fibrose van de nier

Overlevenden van acute respiratory distress syndrome (ARDS) en kritieke ziekte ontwikkelen vaak langdurige spierzwakte. Manuele spiertesten (MMT) is een gestandaardiseerd klinisch onderzoek vaak gebruikt om de kracht van de perifere skeletspieren groepen te meten. Deze video demonstreert MMT met de 6-punts Medical Research Council schaal.

Manuele spiertesten: een methode van meten Extremity spierkracht Toegepast op kritisch zieke patiënten

Sepsis verwijst naar een systemic inflammatory response syndrome als gevolg van een bacteriële infectie, en kan worden gesimuleerd door een chirurgische techniek genoemd cecal ligatie en punctie (CLP). Hier beschrijven we een methode om CLP-geïnduceerde diermodel te gebruiken om geneeskrachtige kruiden te screenen op therapeutische middelen.

Het gebruik van dierlijk model van Sepsis op nieuwe kruidentherapieën Evalueer

Een muis tumor model van chirurgische spanning wordt gebruikt om te onderzoeken hoe postoperatieve onderdrukking van het immuunsysteem bevordert gemetastaseerde ziekte en immuunstimulerende perioperatieve therapie te evalueren.

Een muis Tumor Model of Surgical Stress aan Ontdek de Mechanismen van Postoperatieve Immuunsuppressie en evalueren Novel Perioperative Immuuntherapie

De draad myograph techniek wordt gebruikt om vasculaire vlotte spier functies en de scherm nieuwe drugs te onderzoeken. Wij rapporteren een gedetailleerd protocol voor het meten van de isometrische contractility van de muis mesenterische slagader en voor het screenen van de nieuwe skeletspierrelaxantia van vasculaire glad spierweefsel.

Isometrische Contractility meting van de muis mesenterische slagader met behulp van draad Myography

Leer een gestandaardiseerd muismodel voor omkeerbare unilaterale ureterobstructie om langetermijnfunctionele en structurele nierherstel te evalueren. Deze methode vereenvoudigt chirurgische procedures voor bredere toegankelijkheid in onderzoekslaboratoria.

Een muismodel om de structurele en functionele resultaten op lange termijn te evalueren na de omkering van langdurige unilaterale ureterobstructie (Video) | JoVE Journal

Een muismodel om de structurele en functionele resultaten op lange termijn te evalueren na de omkering van langdurige unilaterale ureterobstructie

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