Name: Kwantificering van violaceïne in Chromobacterium violaceum en de remming ervan door bioactieve stoffen (Video) | JoVE Journal
Uploaded: 2025-08-08T14:10:00.000+00:00
Duration: 7 min 13 s
Description: Ontdek hoe je violaceïne kwantificeert in Chromobacterium violaceum om quorum sensing-remming door bioactieve stoffen te onderzoeken. Deze methode identificeert nieuwe anti-virulentieverbindingen tegen antibiotica-resistente bacteriën.

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<table><tbody><tr><td>DMSO&nbsp;</td><td>Synth</td><td></td><td></td></tr><tr><td>Farnesol</td><td>Sigma-Aldrich</td><td>F203</td><td>95%</td></tr><tr><td>Linalool</td><td>Sigma-Aldrich</td><td>L2602</td><td>97%</td></tr><tr><td>Luria-Bertani Medium</td><td>Difco</td><td>244620</td><td></td></tr><tr><td>Optical Adhesive Film</td><td>Thermo Fisher</td><td>4311971</td><td>MicroAmp</td></tr><tr><td>Resveratrol</td><td>Sigma-Aldrich</td><td>R5010</td><td>&ge;99% (HPLC)</td></tr><tr><td>Synergy HTX multi-mode reader</td><td>Biotek</td><td></td><td></td></tr><tr><td>Tissue Culture Plates 96 well</td><td>Kasvi</td><td>K12-096</td><td></td></tr></tbody></table>

kwantificering van violaceïne in chromobacterium violaceum en de remming ervan door bioactieve stoffen

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Kwantificering van violaceïne in Chromobacterium violaceum en de remming ervan door bioactieve stoffen

Bacteria communicate through a system known as quorum sensing (QS), which allows them to coordinate their behavior in response to changes in cell density. ...

quantification-violacein-chromobacterium-violaceum-its-inhibition

Research

JoVE Journal

Biology

Biologie

Source: Tay, S. B., et al. Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases. J. Vis. Exp. (2016)The video demonstrates a method to quantify biofilm biomass in a pathogenic bacterium, comparing untreated controls with samples treated with a quorum-quenching enzyme. The enzyme disrupts bacterial signaling during early incubation, reducing cell adhesion. After washing and further incubation, control wells form dense biofilms, while treated wells show...

Quantifying Enzyme-Induced Biofilm Suppression in Pathogenic Bacteria

Source: Bucher, T., et al. Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors. J. Vis. Exp. (2016)This video demonstrates a pellicle formation assay using Bacillus subtilis to evaluate small-molecule inhibitors that disrupt matrix synthesis and biofilm development, providing a model to screen compounds that selectively inhibit surface-associated growth without affecting free-floating cells.

Evaluating Inhibitor Effects on Pellicle Formation in Bacillus subtilis

De meeste bacteriële infecties produceren een biofilm. Vanwege hun omgeving zijn biofilm-geassocieerde bacteriën vaak fenotypisch resistent tegen geneesmiddelen. Nieuwe antibacteriële moleculen die bacteriën in biofilms doden, hebben dus een hoge prioriteit. We stellen een test op om snel te screenen op antimicrobiële verbindingen die effectief zijn bij het uitroeien van biofilms.

Gericht op biofilm geassocieerde<em&gt; Staphylococcus aureus</em&gt; Met behulp van Resazurin Gebaseerd Drug-gevoeligheid Assay

We beschrijven een aangepaste methode op basis van agar die is ontworpen om de antischimmeleffecten van plantaardige producten te kwantificeren. Zowel volatiele als niet-volatiele bijdragen aan de antischimmelactiviteit kunnen via dit protocol worden beoordeeld. Bovendien kan de werkzaamheid tegen schimmels worden gemeten in belangrijke ontwikkelingsstadia in één experimentele opstelling.

Meten van vluchtige en niet-vluchtige antischimmelactiviteit van Biocontrol-producten

Dit manuscript beschrijft een methode voor het gebruik van eencellige fluorescentiemicroscopie om bacteriële concurrentie in cocultuur te visualiseren en te kwantificeren.

Kwantificering van interbacteriële concurrentie met behulp van single-cell fluorescentiebeeldvorming

Dit protocol beschrijft de bereiding van het inoculum, de kwantificering van de biofilm op microtiterplaten met behulp van kristalviolette kleurstof, het aantal levensvatbare stoffen in biofilms en de visualisatie van biofilms van Acinetobacter.

Kwantificerings-, levensvatbaarheidsbeoordeling en visualisatiestrategieën voor Acinetobacter-biofilms

Thiofeensulfonamideverbindingen zijn krachtige en specifieke remmers van Vibrio quorum sensing regulatoren LuxR/HapR die hun activiteit in vivo blokkeren, waardoor transcriptie van genen voor virulentie, beweeglijkheid en biofilms wordt voorkomen. Dit protocol beschrijft hoe deze verbindingen worden gesynthetiseerd, gemodelleerd in silico en in vivo worden getest op activiteit tegen LuxR/HapR.

Synthese en bepaling van Vibrio quorum sensing-remmers

Dit protocol beschrijft een eenvoudige en economische methode voor het evalueren van de effectiviteit van potentiële fotosensibilisatoren bij antibacteriële fotodynamische inactivatie (aPDI), met behulp van een plaatformaat met 96 putjes in combinatie met een lichtbron van een LED-paneel. Deze benadering maakt het mogelijk om meerdere experimentele omstandigheden gelijktijdig te testen, waaronder verschillende concentraties, verbindingen en bacteriestammen.

LED-gebaseerde in-vitroscreening voor het beoordelen van fotoactiveerbare moleculen bij bacteriële fotodynamische inactivatie

Dit protocol biedt een systematisch kader om te beoordelen hoe fytochemicaliën de bactericide effecten van traditionele biociden kunnen versterken met behulp van bactericide activiteitsmodellering en combinatorische interactie-assays, die gericht zijn op het identificeren van synergetische mengsels die de microbiële controle verbeteren en het totale biocidegebruik verminderen.

In vitro Evaluatie van de versterkende effecten van plantaardige moleculen op conventionele biociden

Antibioticaresistentie bij adherent-invasieve Escherichia coli-stammen vormt uitdagingen bij het beoordelen van bacteriële invasie met de traditionele gentamicine-beschermingsassay. Er wordt een verbeterde methode beschreven om bacteriële invasie kwantitatief te evalueren door kolonievormende eenheden op te sommen en kwalitatief door geïnfecteerde cellen te visualiseren met immunofluorescentie-confocale microscopie.

Amikacine Beschermingsassay voor Kwantificering en Visualisatie van Escherichia coli-celinvasie

Ontdek hoe je violaceïne kwantificeert in Chromobacterium violaceum om quorum sensing-remming door bioactieve stoffen te onderzoeken. Deze methode identificeert nieuwe anti-virulentieverbindingen tegen antibiotica-resistente bacteriën.

Kwantificering van violaceïne in Chromobacterium violaceum en de remming ervan door bioactieve stoffen

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